131 research outputs found
Optimal combinations of acute phase proteins for detecting infectious disease in pigs
No full textPeer reviewe
Translation regulation after taxol treatment in NIH3T3 cells involves the elongation factor (eEF)2
No full textChanges to the translational machinery that occur during apoptosis have been described in the last few years. The two principal ways in which translational factors are modified during apoptosis are: (i) changes in protein phosphorylation and (ii) specific proteolytic cleavages. Taxol, a member of a new class of anti-tubulin drugs, is currently used in chemotherapeutic treatments of different types of cancers. We have previously demonstrated that taxol induces calpain-mediated apoptosis in NIH3T3 cells [Piñeiro et al., Exp. Cell Res., 2007, 313:369–379]. In this study we found that translation was significantly inhibited during taxol-induced apoptosis in these cells. We have studied the phosphorylation status and expression levels of eIF2a, eIF4E, eIF4G and the regulatory protein 4E-BP1, all of which are implicated in translation regulation. We found that taxol treatment did not induce changes in eIF2α phosphorylation, but strongly decreased eIF4G, eIF4E and 4E-BP1 expression levels. MDL28170, a specific inhibitor of calpain, prevented reduction of eIF4G, but not of eIF4E or 4E-BP1 levels. Moreover, the calpain inhibitor did not block taxol-induced translation inhibition. All together hese findings demonstrated that none of these factors are responsible for the taxol-induced protein synthesis inhibition. On the contrary, taxol treatment increased elongation factor eEF2 phosphorylation in a calpain-independent manner, supporting a role for eEF2 in taxol-induced translation inhibition.Depto. de Farmacología y ToxicologíaFac. de MedicinaTRUEpu
La colección de obras de arte de Juan Piñeiro, fundador del Colegio de la Compañía de Jesús de Pamplona (1580)
No full textEl presente estudio da a conocer la colección de obras de arte de Juan Piñeiro, señor de Eriete y militar navarro al servicio de Carlos V en Italia, formada por escultura devocional, cuadros de temática religiosa, histórica y mitológica, estatuaria clásica, con imágenes de Augusto, Mucio Escévola, Alejandro Magno y los trabajos de Hércules, además de joyas, entre otros objetos. Parte de estas piezas las legó al colegio jesuita de la Anunciada de Pamplona, del que fue fundador y principal benefactor (1580), como seis lienzos religiosos, cuatro de ellos de autoría italiana y una serie de tapices flamencos con la historia de Diana.
The current study lets us know the collection of works of art that belonged to Juan Piñeiro, lord of Eriete and military from Navarre that served Charles V in Italy, formed by devotional sculpture, religious, historical and mythological paintings, classical statuary, with images of Augusto, Mucius Scaevola, Alexander the Great and the labors of Hercules, as well as jewelry, among other items. Piñeiro bequeathed some of these pieces to the Jesuit College of the Annunciation of Pamplona that he founded (1580) and of which he was the main benefactor such as six religious paintings, four of them of Italian author and a series of Flemish tapestries with history of Diana
A Reassessment of the Taxonomic Position of Mesosaurs, and a Surprising Phylogeny of Early Amniotes
No full textWe reassess the phylogenetic position of mesosaurs by using a data matrix that is updated and slightly expanded from a matrix that the first author published in 1995 with his former thesis advisor. The revised matrix, which incorporates anatomical information published in the last 20 years and observations on several mesosaur specimens (mostly from Uruguay) includes 17 terminal taxa and 129 characters (four more taxa and five more characters than the original matrix from 1995). The new matrix also differs by incorporating more ordered characters (all morphoclines were ordered). Parsimony analyses in PAUP 4 using the branch and bound algorithm show that the new matrix supports a position of mesosaurs at the very base of Sauropsida, as suggested by the first author in 1995. The exclusion of mesosaurs from a less inclusive clade of sauropsids is supported by a Bremer (Decay) index of 4 and a bootstrap frequency of 66%, both of which suggest that this result is moderately robust. The most parsimonious trees include some unexpected results, such as placing the anapsid reptile Paleothyris near the base of diapsids, and all of parareptiles as the sister-group of younginiforms (the most crownward diapsids included in the analyses). Turtles are placed among parareptiles, as the sister-group of pareiasaurs (and in diapsids, given that parareptiles are nested within diapsids). This unexpected result offers a potential solution to the long-lasting controversy about the position of turtles because previous studies viewed a position among diapsids and among parareptiles as mutually exclusive alternatives
Flat tax reforms in the U.S.: A boon for the income poor
No full textIn this article we quantify the aggregate, distributional and welfare consequences of
two revenue neutral flat-tax reforms using a model economy that replicates the U.S.
distributions of earnings, income and wealth in very much detail. We find that the less
progressive reform brings about a 2.4 percent increase in steady-state output and a
more unequal distribution of after-tax income. In contrast, the more progressive reform brings about a -2.6 percent reduction in steady-state output and a distribution of aftertax income that is more egalitarian. We also find that in the less progressive flat-tax economy aggregate welfare falls by -0.17 percent of consumption, and in the more
progressive flat-tax economy it increases by 0.45 percent of consumption. In both flattax
reforms the income poor pay less income taxes and obtain sizeable welfare gains
Young-OGEMID Author Interview #6: Prof. Dr. Xandra Kramer and Prof. Dr. Laura Carballo Piñeiro (March 2024)
No full textOnline author interview as editors of the book: Research Methods in Private International Law - A Handbook on Regulation, Research and Teaching. It includes a discussion of the background of the book, fousing on research methods and the broader approach of regulation andteaching methods and an in-depth discussion of inter- and multidisciplinary approaches in private international law
Young-OGEMID Author Interview #6: Prof. Dr. Xandra Kramer and Prof. Dr. Laura Carballo Piñeiro (March 2024)
No full textOnline author interview as editors of the book: Research Methods in Private International Law - A Handbook on Regulation, Research and Teaching. It includes a discussion of the background of the book, fousing on research methods and the broader approach of regulation andteaching methods and an in-depth discussion of inter- and multidisciplinary approaches in private international law
Young-OGEMID Author Interview #6: Prof. Dr. Xandra Kramer and Prof. Dr. Laura Carballo Piñeiro (March 2024)
No full textOnline author interview as editors of the book: Research Methods in Private International Law - A Handbook on Regulation, Research and Teaching. It includes a discussion of the background of the book, fousing on research methods and the broader approach of regulation andteaching methods and an in-depth discussion of inter- and multidisciplinary approaches in private international law
Language and Languages in Contemporary Philosophy
No full textLanguage, one of the central themes of 20th-century philosophy, will be addressed by Galician thinker Ramón Piñeiro in several of his writings. This author, who moves in an atmosphere where existentialism reigned, presents us with a conception of language very close to that of other European authors, especially Heidegger
Pig Major Acute-Phase Protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease
No full textIn the present work, we studied the acute phase protein response after
experimental virus infection in pigs. The animals were experimentally
infected with African Swine Fever (ASF) or Aujeszky's disease (AD)
viruses. The clinical course of ASF infection correlated
with increasingly high levels of pig Major Acute-phase Protein (pig-MAP)
(mean value of 6 mg/mL on day 6 post infection (p.i.), from 6 to 9 times
higher than day 0) and sharp apolipoprotein A-I (apo A-I) decrease (mean
value of 0.5 mg/mL, from 4 to 10 times lower than day 0 on day 4 p.i.).
AD-clinical signs appeared at day 3 p.i., both in vaccinated (moderate
clinical signs) and non-vaccinated pigs (severe outcome within 48 h p.i.).
Pig-MAP and apo A-I profiles also followed clinical signs (changing from
0.70 mg/mL to around 3 mg/mL and from around 3 mg/mL to 0.96 mg/mL,
respectively in non-vaccinated animals), with minor changes in concentration
in the vaccinated group. Haptoglobin levels significantly increased in
ASF and AD infected animals (mean maximum values of 2.77 and 3.96 mg/mL,
respectively). Minor differences for the C-Reactive Protein in the case of
ASF were observed, whereas its concentration increased more than 7 times in
AD-infection. The albumin level was not modified in either case. The
correlation of clinical signs to our data suggests the potential use of
pig-MAP and apo A-I in monitoring infections in swine
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