1,774 research outputs found
Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilm development
No full textA current question in biofilm research is whether biofilm-specific genetic processes can lead to differentiation in physiology and function among biofilm cells. In Pseudomonas aeruginosa, phenotypic variants which exhibit a small-colony phenotype on agar media and a markedly accelerated pattern of biofilm development compared to that of the parental strain are often isolated from biofilms. We grew P. aeruginosa biofilms in glass flow cell reactors and observed that the emergence of small-colony variants (SCVs) in the effluent runoff from the biofilms correlated with the emergence of plaque-forming Pf1-like filamentous phage (designated Pf4) from the biofilm. Because several recent studies have shown that bacteriophage genes are among the most highly upregulated groups of genes during biofilm development, we investigated whether Pf4 plays a role in SCV formation during P. aeruginosa biofilm development. We carried out immunoelectron microscopy using anti-Pf4 antibodies and observed that SCV cells, but not parental-type cells, exhibited high densities of Pf4 filaments on the cell surface and that these filaments were often tightly interwoven into complex latticeworks surrounding the cells. Moreover, infection of P. aeruginosa planktonic cultures with Pf4 caused the emergenceof SCVs within the culture. These SCVs exhibited enhanced attachment, accelerated biofilm development, and large regions of dead and lysed cells inside microcolonies in a manner identical to that of SCVs obtained from biofilms. We concluded that Pf4 can mediate phenotypic variation in P. aeruginosa biofilms. We also performed partial sequencing and analysis of the Pf4 replicative form and identified a number of open reading frames not previously recognized in the genome of P. aeruginosa, including a putative postsegregational killing operon
A note on Belyi's theorem for Klein surfaces
No full textSingerman and the first named author have recently developed a real Belyi thoery, leaving open a particular case in the proof of Belyi's theorem for Klein surfaces. We answer their question affirmatively by a descent argument which turns out to extend to a much more general context
Archivist, Archaeologist, Author and the Tactile Window
No full textThe idea that the predominant way of engaging with architecture is through vision is not uncommon but also not always the most appropriate given that buildings are also experienced through tactile interventions. This consequence that emphasises visual aesthetics in order to appreciate and understand architecture probably has much to do with the assumed but rather vaguely defined role of the architect as designer in the practice of architectural design. A resulting misapprehension is that architects designing for visual appreciation think that they are actually designing physical space for embodied tactile engagement.
This prioritisation of vision in the way architects think about and approach design is questioned through the design project of the Tactile Window in which the position of the architect is redefined through inhabiting the roles of archivist, archaeologist and author during the design process.
A 16th century portrait of Queen Elizabeth I known as the Ditchley portrait, currently on display at the National Portrait Gallery is used as the source from which the design of the Tactile Window is derived from and refers back to. Questioning the validity of vision as the sole means of engaging with the work, information about the portrait and working methods gathered from the three carefully chosen positions mentioned above are drawn on and applied to the making of this Tactile Window that becomes an alternative Ditchley portrait. Through exploring the hidden historical and current narratives of and in the existing portrait, the presence of the portrait is alluded to on an alternative physical site. Key to this are the working methods of an invented archival system of design reasoning, the unearthing of archaeological texts and assuming of authorship within the individual frameworks of the roles of archivist, archaeologist and author.
The redefined role of the architect as archaeologist takes onboard the unearthing of associated drawings and writings as well as the methods of organising and applying the recovered information to the system set up by the archivist. This analysis of the graphic and text based information is used to formulate historical narratives that are woven into the design project. Whereas traditional archaeology stresses on the study of a site from a site with quantifiable limits to the physical context, the notion of archaeological sites in this instance refers to the places where the stored information is unearthed. Through the careful process of archiving and analysing this information, a new site that is located within both the physical and historical contexts of interest is discovered. The author then draws upon the elements in the archival system that includes the findings of the archaeologist to construct the alternative Ditchley portrait in this new site of the Echoing Cedar, the result of which bears no visual resemblance to the existing work.
The Tactile Window is a reading of the Ditchley portrait in which information about and in the painting is transformed into a design proposal for an inhabited structure. The intended method of interaction with this alternative portrait is not merely restricted to vision but relies on engagement with the other senses. This experience is enhanced by the interplay with certain site conditions such as wind and rain in order to allude to specific aspects of the Ditchley portrait that are not visually apparent in the existing work.
In the processes of excavating, finding and revealing the hidden information to create this alternative portrait, the effects of the visuals afforded by the existing portrait inadvertently begin to fade as the validity of a single means of visual expression is questioned
India’s women and the writing process: Interview with Manju Kapur.
No full textLisa Lau interviews Manju Kapur, author of five novels (Difficult Daughters, 1998; A Married Woman, 2003; Home, 2006; The Immigrant, 2008; Custody, 2011) and editor of Shaping the World (2014)
Measurements of heat treatment effects on bovine cortical bones by Nanoindentation and compression testing
No full textAuthor name used in this publication: Lau, Kin-Tak.Version of RecordPublishedC
Lau fish taxonomy
No full textThis thesis is a preliminary attempt to consolidate materials pertaining to Lau fish taxonomy (North Malaita, Solomon Islands). Data utilized come from two sources: those collected by Maranda and Maranda (1966-1968) and those collected by the author during a two-month field period (October-December 1975).
Two approaches to the analysis of terminological systems are explored first. A general description of the Lau Taxonomic Universe follows in which the major components are indicated.
The focus then shifts to a more detailed discussion of Lau Fish taxonomy. Material presented here takes three forms:
(1) A comprehensive list of fish identified according to biological classifications.
(2) A summary of data obtained from informants' Memory Lists of fish names. The problems of taxonomic inclusion and equivalence are considered.
(3) A discussion of those data traditionally regarded as "Non-Taxonomic Terminology".
Distinctive Features are then considered and some examples given. Upper Level Taxa are discussed first. Following this, the Features and Criteria for the classification of Lower Level Taxa are outlined.
Suggestions for further inquiry and propositions concerning analytical avenues constitute the final portion of this presentation.Arts, Faculty ofAnthropology, Department ofGraduat
Sistem Informasi Desa Lau Pakam Kecamatan Mardingding Kabupaten Karo Berbasi Web
No full textThe development of technology today is very rapid with the support of sophisticated
electronic equipment, namely Smartphones, which can access various information
easily. The people of Lau Pakam sometimes find it difficult to get accurate
information about government, especially in the village. And the government that
works in the village sector is difficult to provide information about the village or the
village directly for the Lau Pakam people who need information. Therefore, the
author created a "Web-Based Information System for Lau Pakam Village,
Mardingding District, Karo Regency" to make it easier for the Lau Pakam
community to get information and the village government to provide information.
This system is based on a website to make it easier because it can be accessed by
anyone.41 HalamanKertas Karya Diplom
Stereology of the thyroid gland in Indo-Pacific bottlenose dolphin (Tursiops aduncus) in comparison with human (Homo sapiens) : quantitative and functional implications
No full textAuthor name used in this publication: Kot, Brian Chin Wing.Author name used in this publication: Lau, Thomas Yue HuenVersion of RecordPublishedC
Submarine back-arc lava with arc signature : Fonualei Spreading Center, northeast Lau Basin, Tonga
No full textAuthor Posting. © American Geophysical Union, 2008. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 113 (2008): B08S07, doi:10.1029/2007JB005451.We present major, volatile, and trace elements for quenched glasses from the Fonualei Spreading Center, a nascent spreading system situated very close to the Tofua Volcanic Arc (20 km at the closest), in the northeast Lau Basin. The glasses are basalts and basaltic andesites and are inferred to have originated from a relatively hot and depleted mantle wedge. The Fonualei Spreading Center shows island arc basalt (IAB) affinities, indistinguishable from the Tofua Arc. Within the Fonualei Spreading Center no geochemical trends can be seen with depth to the slab and/or distance to the arc, despite a difference in depth to the slab of >50 km. Therefore we infer that all the subduction-related magmatism is captured by the back arc as the adjacent arc is shut off. There is a sharp contrast between the main spreading area of the Fonualei Spreading Center (FSC) and its northernmost termination, the Mangatolu Triple Junction (MTJ). The MTJ samples are characteristic back-arc basin basalts (BABB). We propose that the MTJ and FSC have different mantle sources, reflecting different mantle origins and/or different melting processes. We also document a decrease in mantle depletion from the south of the FSC to the MTJ, which is the opposite to what has been documented for the rest of the Lau Basin where depletion generally increases from south to north. We attribute this reverse trend to the influx of less depleted mantle through the tear between the Australian and the Pacific plates, at the northern boundary of the Lau Basin.NSK acknowledges the
support of an A.E. Ringwood Scholarship from the RSES
Tan tao du shi he nong cun xue ling er tong zhi jian xiao chuan fa bing lu de cha ju
No full textWong, Lau Yi.Thesis M.Phil. Chinese University of Hong Kong 2015.Includes bibliographical references (leaves 130-171).Abstracts and appendix 1 also in Chinese.Title from PDF title page (viewed on 31, October, 2016).Wong, Lau Yi
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