177,605 research outputs found
I Problemi di Cassio Iatrosofista. Testo critico, introduzione, apparato critico, traduzione e note
No full textEdizione critica, con traduzione e note esegetiche. R. Masullo ha curato del testo la traduzione, l'apparato delle fonti e gli indic
AUGUST BOECKH. LA FILOLOGIA COME SCIENZA STORICA. ENCICLOPEDIA E METODOLOGIA DELLE SCIENZE FILOLOGICHE.
No full textNAPOLI: Guida (ITALY
AUGUST BOECKH. LA FILOLOGIA COME SCIENZA STORICA. ENCICLOPEDIA E METODOLOGIA DELLE SCIENZE FILOLOGICHE.
No full textNAPOLI: Guida (ITALY
45. Marino di Neapoli, Vita di Proclo (Testo critico, introduzione, traduzione et commentario, a cura di R. Masullo)
No full textMotte Laurent. 45. Marino di Neapoli, Vita di Proclo (Testo critico, introduzione, traduzione et commentario, a cura di R. Masullo). In: Revue des Études Grecques, tome 100, fascicule 475-476, Janvier-juin 1987. pp. 182-183
The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon sulfolobus solfataricus
No full textA recombinant form of the elongation factor 2 from the archaeon Sulfolobus solfataricus (SsEF-2), carrying the A26G substitution, has been produced and characterized. The amino acid replacement converted the guanine nucleotide binding consensus sequences A-X-X-X-X-G-K-[T,S] of the elongation factors EF-G or EF-2 into the corresponding G-X-X-X-X-G-K-[T,S] motif which is present in all the other GTP-binding proteins. The rate of poly(U)-directed poly(Phe) synthesis and the ribosome-dependent GTPase activity of A26GSsEF-2 were decreased compared to SsEF-2, thus indicating that the A26G replacement partially affected the function of SsEF-2 during translocation. In contrast, the A26G substitution enhanced the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol [Raimo, G., Masullo, M., Scarano, G., & Bocchini, V. (1997) Biochimie 78, 832±837]. Surprisingly, A26GSsEF-2 was able to hydrolyse GTP even in the absence of ethylene glycol; furthermore, the alcohol increased the affinity for GTP without modifying the catalytic constant of A26GSsEF-2 GTPase. Compared to SsEF-2, the affinity of A26GSsEF-2 for [3H]GDP was significantly reduced. These findings suggest that A26 is a regulator of the biochemical functions of SsEF-2. The involvement of this alanine residue in the guanine nucleotide- binding pocket of EF-2 or EF-G is discussed
Estudiar las intersecciones. Género, sexo y raza en las obras de Gloria Anzaldúa
No full textEl artículo analiza la forma en que se cruzan los conceptos de
género, sexualidad y migración en la obra de la escritora y activista
LGBT, Gloria Anzaldúa. Su trabajo nos sirve para poner
de manifiesto los temas analizados en la investigación ‘interseccional’,
un enfoque reciente que se está imponiendo en
la teoría feminista y de las migraciones. En la primera parte,
describimos las características de esta corriente, sus orígenes,
los campos de aplicación y las cuestiones metodológicas. La
segunda parte profundiza en las obras de la escritora chicana,
sobre todo en su concepto de identidad mestiza, para considerar
las dificultades de quien vive la intersección de diferentes
factores de opresión y desigualdad relacionados con el género,
la raza y las preferencias sexuales
Expression in Escherichia coli of thermostable elongation factor I alpha from the archaeon Sulfolobus solfataricus
No full textThe elongation factor 1 alpha from the archaeon Sulfolobus solfataricus (SsEF-1 alpha) was expressed in Escherichia coli and purified. The SsEF-1 alpha gene was amplified by PCR and cloned in the Ndel site of the pT7-7 expression vector, under the control of the promoter of T7 RNA polymerase. Upon induction with isopropyl beta-D-thiogalactopyranoside, the recombinant SsEF-1 alpha (recSsEF-1 alpha) was purified from the E. coli S-100 extract by a two-step procedure. From 1 litre of cell culture, about 2 mg of purified recSsEF-1 alpha was obtained, The N-terminal sequence of the first 30 amino acid residues of recSsEF-1 alpha was identical with that translated from the nucleotide sequence of the corresponding gene, except for the initial residue, which in recSsEF-1 alpha was Ser instead of Met. The M(r) of recSsEF-1 alpha (determined by electrospray MS) was almost coincident with that of the naturally occurring SsEF-1 alpha (SsEF-1 alpha). The thermal-inactivation and thermophilicity profiles of SsEF-1 alpha and recSsEF-1 alpha were identical. Concerning the functional properties, recSsEF-1 alpha was able to support poly(Phe) synthesis in vitro, to bind GDP and GTP and to elicit an NaCl-dependent GTPase activity [Masullo, De Vendittis and Bocchini (1994) J. Biol. Chem. 269, 20376-20379] with the same efficiency as that displayed by SsEF-1 alpha
Otherness and the other sex within the frame story of the thousand and one nights: texts and criticism
No full textThe Arabian Nights is the most famous Medieval Arabic collection of tales that brings together stories from different literary traditions, mainly Indian and Persian. It is a masterpiece of world literature, and there is an extensive body of scholarly work on it. Following its “rediscovery” by the French orientalist A. Galland in the 1700s and the publication of the printed Arabic editions in the 1800s, the collection became subject to prolific criticism. The best-known narrative of the Arabian Nights is the frame story of the collection, and since the late 1970s, a new line of critical scholarship has emerged, providing multiple interpretations of this element of the text. Perhaps inspired by the rewritings of the frame story in modern and contemporary fiction, this new criticism has proposed topical readings which have been inspired by feminist and gender approaches (Grossman, 1980; Malti-Douglas, 1991; Sallis, 1990), psychoanalytic theories (Beaumont, 2002; Clinton, 1985), and sociocultural and postcolonial perspectives (Mernissi, 2001; Shamma 2017). The main subject of these readings falls within the realm of otherness, and specifically of “sexual and gendered otherness”, i.e., the representation of the other and the relationship with the other sex. This research project identifies in the question of otherness the starting point from which to undertake the scrutiny of the academic literature on the frame story and offer a documented and critical overview of the main trends in modern and contemporary scholarship. In order to achieve this goal, this study proposes an innovative methodology which first compares the various versions and translations of the opening narrative of the Arabian Nights, and then investigates the critical readings, comparing them with the version(s) and/or translation(s) of the frame story they have taken into consideration. This preliminary phase of research sees its continuation in a more substantial discussion about how the readings have presented the question of the relationship with the other sex in the opening narrative of the AN. This study is also the first attempt to offer an annotated outline of the modern and contemporary criticism of the frame story concentrating on both English (i.e., written in English) and Arabic (i.e., written in Arabic) scholarship
Expression in Escherichia coli of thermostable elongation factor 1 alpha from the archaeon Sulfolobus solfataricus
No full textThe elongation factor 1 alpha from the archaeon Sulfolobus solfataricus (SsEF-1 alpha) was expressed in Escherichia coli and purified. The SsEF-1 alpha gene was amplified by PCR and cloned in the Ndel site of the pT7-7 expression vector, under the control of the promoter of T7 RNA polymerase. Upon induction with isopropyl beta-D-thiogalactopyranoside, the recombinant SsEF-1 alpha (recSsEF-1 alpha) was purified from the E. coli S-100 extract by a two-step procedure. From 1 litre of cell culture, about 2 mg of purified recSsEF-1 alpha was obtained. The N-terminal sequence of the first 30 amino acid residues of recSsEF-1 alpha was identical with that translated from the nucleotide sequence of the corresponding gene, except for the initial residue, which in recSsEF-1 alpha was Ser instead of Met. The M(r) of recSsEF-1 alpha (determined by electrospray MS) was almost coincident with that of the naturally occurring SsEF-1 alpha (SsEF-1 alpha). The thermal-inactivation and thermophilicity profiles of SsEF-1 alpha and recSsEF-1 alpha were identical. Concerning the functional properties, recSsEF-1 alpha was able to support poly(Phe) synthesis in vitro, to bind GDP and GTP and to elicit an NaCl-dependent GTPase activity [Masullo, De Vendittis and Bocchini (1994) J. Biol. Chem. 269, 20376-20379] with the same efficiency as that displayed by SsEF-1 alpha
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