1,141 research outputs found
Women and independence in the nineteenth century novel : a study of Austen, Trollope and James
No full text'Women
and
independence in the nineteenth century novel : a
study
of
Austen, Trollope
and
James', begins
with the
concept of
independence
and works through the three
most common usages of
the
word.
The first, financial independence (not
needing to
earn one's
livelihood)
appears to be
a necessary prerequisite
for the
second
and third forms
of
independence,
although it is by
no means an
unequivocal good
in
any of
the
novels.
The
second,
intellectual
independence (not
depending
on others
for
one's opinion or conduct;
unwilling
to be
under obligation
to
others),
is
a matter of asserting independence
while employing
terms
which society recognizes.
The third,
of
being independent, is
exemplified
by
an
inward
struggle
for
a
knowledge
of self.
In
order
to trace the development
of
the idea
of self
during the
nineteenth century,
I have
chosen a group of novels which seem
to be
representative of
the beginning, the
middle, and the
end of
the period.
Particular
attention
is
given
to the
characterizations of
Emma
Woodhouse, Glencora Palliser, Isabel Archer, Milly Theale and
Maggie
Verver. Whereas in Jane Austen's
novels
the self
has a
definite shape
which the heroine
must
discover, and
in Anthony Trollope's
novels
the
self
(reflecting
the idea
of socially-determined man) must
learn to
accommodate social and political changes,
in Henry James's
novels
the
self
determined by
external manifestations
(hollow
man)
is
posed
against
the exercise of
the free
spirit or soul.
Jane Austen's
novels
look backward,
as she reacts against
late
eighteenth century romanticism, and
forward,
with
the development
of
the heroine
who exemplifies
intellectual independence. Anthony
Trollope's
women characters are creatures of social and political
adaptation; although
they do
not
derive their
reason
for being
from
men,
they
must accommodate
themselves to
men's wishes.
And
Henry James looks backward,
wistfully, at
Austen's
solid, comforting,
innocent
self and
forward, despairingly, to the dark,
unknowable self
of
the twentieth
century
Handheld-Impedance-Measurement System with seven-decade capability and potentiostatic function
No full textThis paper describes design and test of a new impedance-measurement system for nonlinear devices that exhibits a seven-decade range and works down to a frequency of 0.01 Hz. The system is specifically designed for electrochemical measurements, but the proposed architecture can be employed in many other fields where flexible signal generation and analysis are required. The system employs an unconventional signal generator based on two pulsewidth modulation (PWM) oscillators and an autocalibration system that allows uncertainties of less than 3% to be obtained over a range of 1 kΩ to 100 GΩ. A synchronous demodulation processing allows the noise superimposed to the low-amplitude input signals to be made negligibl
Effect of plasmid design and type of integration event on recombinant protein expression in Pichia pastoris
No full text(syn. ) is one of the most common eukaryotic expression systems for heterologous protein production. Expression cassettes are typically integrated in the genome to obtain stable expression strains. In contrast to , where short overhangs are sufficient to target highly specific integration, long overhangs are more efficient in and ectopic integration of foreign DNA can occur. Here, we aimed to elucidate the influence of ectopic integration by high-throughput screening of >700 transformants and whole-genome sequencing of 27 transformants. Different vector designs and linearization approaches were used to mimic the most common integration events targeted in Fluorescence of an enhanced green fluorescent protein (eGFP) reporter protein was highly uniform among transformants when the expression cassettes were correctly integrated in the targeted locus. Surprisingly, most nonspecifically integrated transformants showed highly uniform expression that was comparable to specific integration, suggesting that nonspecific integration does not necessarily influence expression. However, a few clones
Predictors of long-distance race performance in master runners
No full textPeak aerobic power (V ̇O2peak) and parameters related to training are associated with long-distance running performance in master athletes. Running economy (RE) predicts performance in younger runners, but its relationship to racing ability in older athletes is unclear. Allometrically scaled RE (alloV ̇O2; ml kg-0.66 min-1), energy cost (EC; kcal kg-1 km-1), and percent of V ̇O2peak (%V ̇O2peak) required in a submaximal bout represent RE more accurately than V ̇O2 does. The VDOT score, estimating V ̇O2peak and RE, can be used to compare races of different distances. Purpose: To determine predictors of temperature-converted VDOT in master runners training for a long-distance race (10-26.2 mi). Methods: Twenty-three master runners (age 57±9 years; eight females) performed treadmill marathon-intensity-effort (MIE) and V ̇O2peak tests within four weeks of their goal race. The MIE occurred at 88% of predicted maximum heart rate, which corresponds to estimated marathon intensity. Participants completed online training-history surveys. Forward stepwise multiple linear regression was used to find key predictors of VDOT. The alpha level for significance was 0.05. Results: Converted VDOT was significantly associated with 3-year peak weekly training distance (3YP) (r = 0.454, p = 0.039), V ̇O2peak (r = 0.845, p = 0.000), alloV ̇O2 (r = 0.623, p = 0.005), and EC (r = -0.528, p = 0.018). The best-fitting model included V ̇O2peak and 3YP (r = 0.898). Conclusion: Physiological and training factors are related to race performance in master runners. The best predictors of VDOT are V ̇O2peak and 3YP. Training to enhance these variables may improve distance-running performance in masters.This study was funded by NIH grant R01 HL208962-05
Construction of FcHyd5p Variants to Assess the Potential of These Class II Hydrophobins as Molecular Tools for Surface Modification
No full textHydrophobins represent an attractive surface modification technology that can be paired with a variety of fusion partners to increase their versatility. Surface modification is a technique widely used to influence the adhesion of molecules to surfaces. The aim of this project is to assess the potential of the class II HFB FcHyd5p from Fusarium culmorum as a surface modification technique. FcHyd5p variants bearing RGD with two different linker sequences were engineered. Proteins were expressed in Pichia pastoris and purified using fast protein liquid chromatography (FPLC) and affinity chromatography. Bis-Tris-SDS-PAGE confirmed that native and fusion proteins were successfully produced and purified, with yields of the fusion constructs exceeding that of the native. Water contact angle demonstrated that RGD-FcHyd5p constructs could convert surface wettability of siliconized glass. Quartz crystal microbalance with dissipation (QCM-D) quantified HFB adsorption and assessed the effect of the presentation of RGD through different linkers on cell adhesion.M.A.S
Construction of FcHyd5p Variants to Assess the Potential of These Class II Hydrophobins as Molecular Tools for Surface Modification
No full textHydrophobins represent an attractive surface modification technology that can be paired with a variety of fusion partners to increase their versatility. Surface modification is a technique widely used to influence the adhesion of molecules to surfaces. The aim of this project is to assess the potential of the class II HFB FcHyd5p from Fusarium culmorum as a surface modification technique. FcHyd5p variants bearing RGD with two different linker sequences were engineered. Proteins were expressed in Pichia pastoris and purified using fast protein liquid chromatography (FPLC) and affinity chromatography. Bis-Tris-SDS-PAGE confirmed that native and fusion proteins were successfully produced and purified, with yields of the fusion constructs exceeding that of the native. Water contact angle demonstrated that RGD-FcHyd5p constructs could convert surface wettability of siliconized glass. Quartz crystal microbalance with dissipation (QCM-D) quantified HFB adsorption and assessed the effect of the presentation of RGD through different linkers on cell adhesion.M.A.S
What A Waste: Redefining the Private-, Collective-, and Public Space Towards a Circular System for Food and Consumer Goods in the Domestic Domain
No full textThe scarcity of resources is increasing, resulting in the transformation of entire regions. People take a lot of the resources on Earth for granted. This calls for a transition from the current ‘throw-away’-economy to a ‘circular’ economy. The constant circulation of materials and products in this type of system preserves the value of materials. Academics and practitioners are addressing this transition, but they frequently ignore its social and spatial aspects. A socio-spatial framework for reorganizing a district towards a circular system is proposed in this thesis. The solution presented is fundamentally utopian, aiming to challenge established ideas about the current system. In this thesis, the public space’s redesign serves as an entry point to the private and communal spaces. The design location for this thesis is the Indische Buurt. This district is located in east of Amsterdam. With a large proportion of social housing, the area’s primary use is residential. Additionally, there are a few shopping streets nearby. The approach used in this thesis was context-specific. The three main approaches used were literature reviews, field research, and ‘research by design’. According to the analysis, residents are crucial in deciding whether and how to dispose of things. But at the moment, the area doesn’t have many resources supporting circular behavior. The issue of waste disposal in public areas is one of the effects. In addition, the district has a high proportion of minima-households, little greenery, and roadways that are mostly occupied by parked cars. The primary observation about governance analysis is the lack of emphasis on the spatial implications necessary for the shift to a circular system. The district is reorganized using a spatial framework that was constructed using the R-ladder. The R-ladder is a circularity model made up of ten R-strategies for reducing the amount of materials and resources used in product chains. The higher up the strategy is on the ladder, the more value is retained. Following that, a spatial design for a square and its surrounding streets is proposed. The visibility of circularity is prioritized in this design to enhance public awareness. Furthermore, the design aims to maximize the value retention of materials or products by selecting the solution that best retains value. In addition to the design, recommendations will be made to the governance of the Netherlands and Amsterdam. This will improve the existing circular strategy and policy.Architecture, Urbanism and Building Sciences | Urbanis
Enzymatic upgrading of heteroxylans for added-value chemicals and polymers
No full text| openaire: EC/H2020/648925/EU//BHIVE Funding Information: This work was supported by Genome Canada for the project ‘SYNBIOMICS - Functional genomics and techno-economic models for advanced biopolymer synthesis’ (LSARP, grant number 10405 ), and the European Research Council (ERC) Consolidator Grant ( BHIVE- 648925 ). Publisher Copyright: © 2021 The Author(s)Xylan is one of the most abundant, natural polysaccharides, and much recent interest focuses on upgrading heteroxylan to make use of its unique structures and chemistries. Significant progress has been made in the discovery and application of novel enzymes for debranching and modifying heteroxylans. Debranching enzymes include acetylxylan esterases, α-L-arabinofuranosidases and α-Dglucuronidases that release side groups from the xylan backbone to recover both biochemicals and less substituted xylans for polymer applications in food packaging or drug delivery systems. Besides esterases and hydrolases, many oxidoreductases including carbohydrate oxidases, lytic polysaccharide monooxygenases, laccases and peroxidases have been also applied to alter different types of xylans for improved physical and chemical properties. This review will highlight the recent discovery and application of enzymes for upgrading xylans for use as added-value chemicals and in functional polymers.Peer reviewe
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