3,116 research outputs found
Suggestive evidence that the highly metastatic variant ESb of the T-cell lymphoma Eb is derived from spontaneous fusion with a host macrophage
No full textTwo lines of evidence are reported which suggest that the highly metastatic variant ESb of the T-cell lymphoma Eb is derived from spontaneous fusion with a host macrophage. Firstly, ESb cells are shown to express the macrophage differentiation antigen Mac-1 which was not found on Eb cells or on any other tumor cells tested except the macrophage tumor line Pu5. Secondly, the progression from low to high metastatic capacity could be reproduced in vitro following hybridization of thioguanine-resistant Eb cells (EbTGR) with syngeneic bone-marrow-derived macrophages. Two HAT medium-selected hybrid tumor lines (Eb-F1 and Eb-F2) could be established. They were found to express cell surface markers of both parental lines: T lymphoid differentiation antigens from T-lymphoma and macrophage antigens (Mac-1, class II MHC antigens) from the normal cell fusion partner. The antigens were identified on the hybrids and subclones thereof by means of monoclonal antibodies and 3 different detection assays: cytofluorography, complement-dependent cytotoxicity and immunoprecipitation followed by gel electrophoresis. Animals inoculated s.c. with the parental line EbTGR developed local tumors but not metastases and survived for more than 40 days. In contrast, animals inoculated similarly with Eb-F1 or Eb-F2 cells quickly developed metastases in visceral organs and died as early as 10-14 days following inoculation. In many but not all respects, the in vitro-derived T-lymphoma-macrophage hybrids resembled the spontaneous in vivo-derived variant ESb. These findings, together with the presence of Mac-1 antigen on ESb cells, suggest (1) that ESb variant cells may be derived from spontaneous fusion with a host cell, most likely a macrophage and (2) that somatic cell fusion may be an important mechanism of genetic rearrangements leading to metastatic variants. The new highly metastatic tumor lines which were developed under well-defined in vitro conditions, and their subclones, may become very useful tools for studying the contribution of specific genetic traits and of membrane-related structures to various steps of the metastatic process
The psychological functioning of children with Epidermolysis Bullosa (EB) and its relationship with specific aspects of disease
No full textEpidermolysis Bullosa (EB) is a group of genetic conditions resulting in skin and mucosal membrane fragility. EB is characterised by chronic wounds and scarring, consequent functional limitations and high levels of pain. In its most severe forms, life expectancy is significantly foreshortened
AUTHOR CONTRIBUTION
No full textConception or design of the study: Silva AC, Silva LG, Souza ARS, Martins, AKL, Gomes EB. Data collection: Silva AC, Silva LG, Souza ARS. Analysis and interpretation of the data: Silva AC, Silva LG, Souza ARS. Writing of the article or critical review: Silva AC, Gomes EB. Final approval of the version to be published: Silva AC, Martins, AKL, Oliveira CJ, Alencar AMPG, Gomes EB
Supporting safe motherhood : a review of financial trends : summary
No full textAn estimated 500,000 women, 99 percent of them from the developing world, die each year from pregnancy-related causes. About three quarters of these deaths are the direct result of obstetrical complications -- hemorrhage, infection, toxemia, obstructed labor, and abortion (under primitive and illegal conditions). An estimated equivalent number of infants do not survive their mother's death. For surviving mothers, the consequences of pregnancy have a severe impact on health and family economics. The strategy for safe motherhood is based on two approaches. First, the encouragement of activities that indirectly improve maternal health. These include education, policies to improve women's rights and working conditions, health care and nutrition, transportation and communication systems, water and sanitation facilities, and increases in family income and food production. The second approach targets activities to reduce maternal deaths. These activities include reducing unwanted pregnancies through the provision of family planning services, and through national policies that recognize the importance of this issue. A second objective is to reduce the risks of pregnancy through providing community-based family planning and prenatal services to identify high-risk cases'adequate referral services for the complications of pregnancy, and communication and transport systems to support patient referral procedures.Health Monitoring&Evaluation,Health Systems Development&Reform,Gender and Health,Early Child and Children's Health,Agricultural Knowledge&Information Systems
AUTHOR CONTRIBUTION
No full textConception or design of the study: Silva AC, Silva LG, Souza ARS, Martins, AKL, Gomes EB. Data collection: Silva AC, Silva LG, Souza ARS. Analysis and interpretation of the data: Silva AC, Silva LG, Souza ARS. Writing of the article or critical review: Silva AC, Gomes EB. Final approval of the version to be published: Silva AC, Martins, AKL, Oliveira CJ, Alencar AMPG, Gomes EB
AUTHOR CONTRIBUTION
No full textConception or design of the study: Silva AC, Silva LG, Souza ARS, Martins, AKL, Gomes EB. Data collection: Silva AC, Silva LG, Souza ARS. Analysis and interpretation of the data: Silva AC, Silva LG, Souza ARS. Writing of the article or critical review: Silva AC, Gomes EB. Final approval of the version to be published: Silva AC, Martins, AKL, Oliveira CJ, Alencar AMPG, Gomes EB
Endogenous Technology Adoption and R&D as Sources of Business Cycle Persistence
No full textWe examine the hypothesis that the slowdown in productivity following the Great Recession was in significant part an endogenous response to the contraction in demand that induced the downturn. We motivate, develop and estimate a model with an endogenous TFP mechanism that allows for costly development and adoption of technologies. Our main finding is that a significant fraction of the post-Great Recession fall in productivity was an endogenous phenomenon, suggesting that demand factors played an important role in the post-crisis slowdown of capacity growth. More generally, we provide insight into why recoveries from financial crises may be so slow
EB-KG: Knowledge Graph of the first 8 eiditions Encyclopaedia Brittanica (1768-1860)
No full textThis Knowlege Graph represents the information of the first eight editions of Encyclopaedia Brittanica (years: 1768 to 1860) in RDF (ttl format).
The raw dataset is provided by the NLS in this link , and it comprises of eight editions and a total of 195 volumes with a total size of 44GB. It uses two XMLs schemas: METS for descriptive, structural, technical and administrative metadata (Title, Author, Publisher, etc); and ALTO for encoding the OCR text of a page.
In this work, we have extracted the information from METS and ALTO XMLS using defoe tool and developed novel information extraction heuristics. With the extracted information, we created the EB-KG Knowlege Graph, which uses the EB Ontolgy, to represent such information. Furthermore, during the information extraction phase, we have employed several techniques to mitigate two common OCR errors: long-S and the line-break hyphenation.
The EB-KG contains 1,638,239 RDF triples. It has information from 8 editions. Each edition can have several Volumes, references to Books, Supplements; it also has an Editor and a Publisher, which can be a Person or an Organization. A Volume has several Pages, which can contain several Terms. And a Term can be either a Topic (a term described across several pages, often combining text, pictures, and tables.) or an Article (a description of the term in one- or two-paragraph long text (similar to an entry in a dictionary)). The data model of the EB-KG can be found here.
The original ALTO files do not indicate the start and end of each EB term, the first part of our work involved the
automated extraction of all terms (along with their metadata) across editions, so they can be analysed independently without the surrounding text.This work was performed during my 2021-2022 National Library of Scotland Digital Scholarship Fellowship
Effect of build location on microstructural characteristics and corrosion behavior of EB-PBF built Alloy 718
No full textElectron beam-powder bed fusion (EB-PBF), a high-temperature additive manufacturing (AM) technique, shows great promise in the production of high-quality metallic parts in different applications such as the aerospace industry. To achieve a higher build efficiency, it is ideal to build multiple parts together with as low spacing as possible between the respective parts. In the EB-PBF technique, there are many unknown variations in microstructural characteristics and functional performance that could be induced as a result of the location of the parts on the build plate, gaps between the parts and part geometry, etc. In the present study, the variations in the microstructure and corrosion performance as a function of the parts location on the build plate in the EB-PBF process were investigated. The microstructural features were correlated with the thermal history of the samples built in different locations on the build plate, including exterior (the outermost), middle (between the outermost and innermost), and interior (the innermost) regions. The cubic coupons located in the exterior regions showed increased level (~ 20 %) of defects (mainly in the form of shrinkage pores) and lower level (~ 30-35 %) of Nb-rich phase fraction due to their higher cooling rates compared to the interior and middle samples. Electrochemical investigations showed that the location indirectly had a substantial influence on the corrosion behavior, verified by a significant increase in polarization resistance (Rp) from the exterior (2.1 ± 0.3 kΩ.cm2) to interior regions (39.2 ± 4.1 kΩ.cm2). © 2020, The Author(s)
Comparison of culture methodology for the detection of methicillin-resistant Staphylococcus pseudintermedius in clinical specimens collected from dogs
No full textMethicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a major pathogen in dogs and has been implicated as a hospital-acquired pathogen in veterinary hospitals. We attempted to determine if selective culture methods will detect more MRSP when compared to the traditional culture methods in clinical samples from dogs in Atlantic Canada with a high risk for MRSP infection. Each sample was tested using 4 culture methods: traditional culture; mannitol salt agar with 2 μg/mL of oxacillin (MSAox); enrichment broth (EB) with MSAox; and EB with traditional culture. Detection of penicillin-binding protein 2', via latex agglutination, was used as a confirmatory test for oxacillin resistance. We analyzed 741 samples from 556 dogs between February 2013 and April 2014. The prevalence of MRSP in samples detected by any method was estimated at 13.4% (95% CI: 11.1-16.0%). When the prevalence of MRSP was determined according to culture method, EB with MSAox detected the highest prevalence (11.2% [9.1-13.7%]), followed by EB with traditional (10.8% [8.8-13.2%]), traditional (10.1% [8.1-12.5%]), and MSAox (8.9% [7.1-11.2%]). The prevalence using the traditional culture method did not differ significantly from any of the 3 selective culture methods. Culture with MSAox detected significantly fewer MRSP than either of the EB methods. The addition of EB to current methodology is recommended, particularly for patients considered at high risk for MRSP infection
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