419 research outputs found
Sequence alignment of RecA family proteins from ( RadA), (RadA), (Rad51), (Rad51 and Dmc1), (Dmc1 and ScRad51) and (RecA)
No full text<p><b>Copyright information:</b></p><p>Taken from "Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins"</p><p></p><p>Nucleic Acids Research 2007;35(6):1787-1801.</p><p>Published online 28 Feb 2007</p><p>PMCID:PMC1874592.</p><p>© 2007 The Author(s)</p> All these RecA-like strand exchange proteins have similar N-terminal domains. The C-terminal RecA domains have been removed for clarity. Secondary structural features of the left-handed RadA helical filament are indicated in cyan (α helices) and red (β strands). Functional motifs are indicated under their corresponding amino acid sequences: the putative dsDNA binding HhH motif, the putative ssDNA binding L1 and L2 loops, the ATP binding Walker A and B motifs, the polymerization motif (PM), the subunit rotation motif (SRM), and others. Positions of the R–E–E triad are indicated using blue arrows
Evaluation of the referral, counter-referral and authorization system of the regional health insurance of an institution of the exceptional regime
No full textLa Regional de Aseguramiento en Salud No. 1, perteneciente a una entidad del régimen de excepción del sector salud en Colombia, presenta dificultades en el sistema de referencia y contrarreferencia, cuyo propósito es coadyuvar en la gestión administrativa para la adecuada prestación al usuario de las atenciones en salud siendo oportunas e integrales, conforme a cada nivel y grado de complejidad. Esto denota la necesidad de realizar un estudio de integración de métodos combinando la metodología cualitativa y cuantitativa obteniendo una convergencia en los resultados aplicados al proceso de referencia, contrarreferencia y autorizaciones. Los instrumentos usados sirvieron para evaluar las actividades que cumple el talento humano coordinador del proceso, la documentación de los procedimientos, el sistema de información utilizado, el análisis de las PQRS correspondiente a los periodos del IV trimestre del año 2023 y I trimestre del 2024. Los resultados obtenidos muestran deficiencias en el acceso y oportunidad en los servicios, situación que subraya la necesidad de recomendar acciones en la modernización de los sistemas de información, optimizar los procesos administrativos y fortalecer los programas de capacitación al personal responsable, dadas las barreras para garantizar satisfacción al usuario.Magíster en Administración en SaludMaestríaThe Regional Health Assurance Regional No. 1, belonging to an entity of the exceptional regime of the health sector in Colombia, presents difficulties in the referral and counter-referral system, whose purpose is to assist in the administrative management for the adequate provision of timely and comprehensive health care to the user, according to each level and degree of complexity. This shows the need to carry out a study of integration of methods combining qualitative and quantitative methodology, obtaining a convergence in the results applied to the process of referral, counter-referral and authorizations. The instruments used were used to evaluate the activities carried out by the human talent coordinating the process, the documentation of the procedures, the information system used, the analysis of the PQRS corresponding to the periods of the IV quarter of the year 2023 and I quarter of 2024. The results obtained show deficiencies in access and timeliness of services, a situation that underscores the need to recommend actions to modernize information systems, optimize administrative processes and strengthen training programs for the responsible personnel, given the barriers to guarantee user satisfaction
Pyridinecarboxylic acid derivative stimulates pro-angiogenic mediators by PI3K/AKT/mTOR and inhibits reactive nitrogen and oxygen species and NF-κB activation through PPARγ-dependent pathway in T. cruzi-infected macrophages
No full textChagas disease is caused by T. cruzi infection and represents an important public health concern in Latin America. Macrophages are one of the main infiltrating leukocytes in response to infection. Parasite persistence could trigger a sustained activation of these cells, contributing to the damage observed in this pathology, particularly in the heart. HP24, a pyridinecarboxylic acid derivative, is a new PPARγ ligand which exerts anti-inflammatory and pro-angiogenic effects. The aim of this work was to deepen the study of the mechanisms involved in the pro-angiogenic and anti-inflammatory effects of HP24 in T. cruzi-infected macrophages, which have not yet been elucidated. We show for the first time that HP24 increases expression of VEGF-A and eNOS through PI3K/AKT/mTOR and PPARγ pathways, and that HP24 inhibits iNOS expression and NO release, a pro-inflammatory mediator, through PPARγ-dependent mechanisms. Furthermore, this study shows that HP24 modulates H2O2 production in a PPARγ-dependent manner. It is also demonstrated that this new PPARγ ligand inhibits the NF-κB pathway. HP24 inhibits IKK phosphorylation and IκB-α degradation, as well as p65 translocation to the nucleus in a PPARγ-dependent manner. In Chagas disease, both the sustained increment in pro-inflammatory mediators and microvascular abnormalities are crucial aspects for the generation of cardiac damage. Elucidating the mechanism of action of new PPARγ ligands is highly attractive, given the fact that it can be used as an adjuvant therapy, particularly in the case of Chagas disease in which inflammation and tissue remodeling play an important role in the pathophysiology of this diseas
Subunit rotation influences the quaternary structures of archaeal RadA and Rad51 proteins
No full text<p><b>Copyright information:</b></p><p>Taken from "Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins"</p><p></p><p>Nucleic Acids Research 2007;35(6):1787-1801.</p><p>Published online 28 Feb 2007</p><p>PMCID:PMC1874592.</p><p>© 2007 The Author(s)</p> The structures of the left-handed RadA helical filament (in cyan), the Rad51 closed ring (in red), the right-handed RadA-AMPPNP helical filament (in green) and the overwound right-handed RadA helical filament (in pink) are superimposed by fixing the N-terminal domain (NTD, in yellow) and polymerization motif (PM). The side chain of phenylalanine residue of PM is indicated in green. The Core ATPase domain (CAD) of neighboring protomers is indicated in gray. The subunit rotation motif (SRM) locates immediately after the α5 helix. AMP-PNP in the right-handed RadA-AMPPNP helical filament is also marked by arrow, respectively. The disposition of adjacent α11 and α12 helices are shown to illustrate subunit rotation
Does strict employment protection discourage job creation? Evidence from Croatia
No full textEmployment protection legislation in Croatia is among the most strict in Europe. Firing is difficult and costly, and flexible forms of employment are limited. Is this apparent rigidity reflected-as one would expect based on standard economic theory-in low labor market dynamics? Is job creation low and hiring limited? Is the job security of insiders achieved at the cost of outsiders not being able to enter thelabor market? The author attempts to answer these questions by examining job flows. If the employment protection legislation is binding, then job and worker turnover should be low. He shows that this is indeed the case. Hiring is limited and the average job tenure is very long in Croatia. Job destruction is low, however job creation is still lower. The result is accumulation of unemployment, in large part due to new labor market entrants not being able to find a job. The high degree of job protection also seems to strengthen the bargaining position of insiders and results in relatively high wages. So, wages in Croatia are higher than among its competitors, even after adjusting for productivity. These high labor costs are likely to contribute to limited job creation in existing firms, but also are likely to discourage the entry of-and thus job creation in-new firms. The author presents evidence that firm growth has been indeed limited in Croatia, contributing to the low employment level. The author examines other potential causes of high unemployment in Croatia (the unemployment benefit system, labor taxation, the wage structure, and skill and spatial mismatches). He argues that they do not play a substantial part in accounting for poor labor market outcomes in Croatia. The author concludes that the stringent employment protection legislation is the key labor market institution behind low job creation and high unemployment. Based on this he recommends specific measures aimed at liberalizing the labor market to foster job creation and employment.Labor Management and Relations,Labor Policies,Labor Markets,Environmental Economics&Policies,Trade Finance and Investment,Labor Markets,Labor Management and Relations,Labor Standards,Banks&Banking Reform,Environmental Economics&Policies
FIG. 6 in Evolution In The Genus Rhinella: A Total Evidence Phylogenetic Analysis Of Neotropical True Toads (Anura: Bufonidae)
No full textFIG. 6. Hyoid plate: A, Rhinella cristinae ICN 26233 (char. 25.0), B, R . manu MHNC 4404 (char. 25.1). Arrowheads indicate the occurrence of posterior lobes of the anterolateral processes in B. Panels redrawn from Vélez-R. and Ruiz-C., 2002 (A); Chaparro et al., 2007 (B).Published as part of Pereyra, Martín O., Blotto, Boris L., Baldo, Diego, Chaparro, Juan C., Ron, Santiago R., Elias-Costa, Agustín J., Iglesias, Patricia P., Venegas, Pablo J., C. Thomé, Maria Tereza, Ospina-Sarria, Jhon Jairo, Maciel, Natan M., Rada, Marco, Kolenc, Francisco, Borteiro, Claudio, Rivera-Correa, Mauricio, Rojas-Runjaic, Fernando J.M., Moravec, Jiří, De La Riva, Ignacio, Wheeler, Ward C., Castroviejo-Fisher, Santiago, Grant, Taran, Haddad, Célio F.B. & Faivovich, Julián, 2021, Evolution In The Genus Rhinella: A Total Evidence Phylogenetic Analysis Of Neotropical True Toads (Anura: Bufonidae), pp. 1-157 in Bulletin of the American Museum of Natural History 2021 (447) on page 26, DOI: 10.1206/0003-0090.447.1.1, http://zenodo.org/record/541275
Zborsko dirigiranje s posebnim osvrtom na dirigiranje dječjim i mješovitim zborom
No full textRad obrađuje probleme vođenja dječjeg i mješovitog zbora (organizacija zbora, vrste dječjeg zbora, kako provesti audiciju, tehnike disanja i uzimanja daha, artikulacija, intonacij, ritmički problemi, glazbena fraza, rad na partituri). Na kraju rada obrađeni su primjeri skladbi - Ave Maria Zoltana Kodalyja za dječji zbor, te Adoramus Te W. A. Mozarta i Nachtwache I. J. Brahmsa za mješoviti zbor
Trailblazing through a Knowledge Space of Science: Forward Citation Expansion in CiteSeer
No full textUnderstanding emerging trends and patterns in science and technology is essential not only to scientists and engineers in their own fast-advancing fields but also to a wide variety of individuals and organizations who are also interested in tracking the development of thematic topics. This is a challenging task because many existing tools are not particularly designed to deal with the dynamics of intellectual structures that transcend the boundaries of individual documents or isolated topics. In this article, we introduce a conceptual and operational platform that extends the traditional notion of traveling along individual citation pathways and defines operators for recursive and holistic theme expansion based on citation connectivity. We describe the implementation of a forward expansion operator and illustrate its potential with the CiteSeer metadata. In addition, we integrate the forward expansion operators with information visualization techniques
An initial exploration of the link relationship between UK university Web sites.
No full textAggregates of links are of interest to information scientists in the same way as citation counts are: as potential sources of data from which new knowledge can be mined. Builds on the recent discovery of a correlation between a Web link count measure and the research quality of British universities by applying a range of multivariate statistical techniques to counts of links between pairs of universities. This represents an initial attempt at developing an understanding of this phenomenon. Extracts plausible results. Also identifies outliers in the data by the techniques, some of which were verified by being tracked down to identifiable Web phenomena. This is an important outcome because successful anomaly identification is a precondition to more effective analysis of this kind of data. The identification of groupings is encouraging evidence that Web links between universities can be mined for significant results, although it is clear that more methodological development is needed, if any but the simplest patterns are to be extracted. Finally, based upon the types of patterns extracted, argues that none of the methods used are capable of fully analysing link structures on their own
The coastal marine mollusks from northern Macanao Peninsula, Margarita Island, Venezuela
No full textEn este estudio se analiza la composición y distribución de los moluscos marinos litorales del norte de la península de Macanao, Isla de Margarita, recolectados entre febrero y mayo de 2007 en trece localidades. Las colectas se hicieron manualmente y con equipo ligero de submarinismo. Se estudiaron 2270 ejemplares, distribuidos en 68 familias, 106 géneros y 152 especies, representantes de las clases Bivalvia Linnaeus, 1758, Gastropoda Cuvier, 1797 y Polyplacophora Gray, 1821. Los gasterópodos fueron la clase dominante con 89 especies, siendo las especies con mayor distribución Lottia antillarum (Sowerby, 1831) y Purpura patula (Linnaeus, 1758) con 84,6%. En bivalvos (55) las especies con mayor distribución fueron Isognomom bicolor (C.B. Adams, 1845) con una presencia de 76,9% y Arca zebra (Swainson, 1833) con 69,2%; y en los polyplacophoros Chiton marmoratus Gmelin, 1791 y Chiton tuberculatus Linné, 1758 con 69,2% y Acanthopleura granulata (Gmelin, 1791) con 53,8%. Los valores más altos de diversidad, equidad y riqueza se obtuvieron en la localidad VII de El Faro de Punta Tigre II con un H´ de 3,8 bits/ind, J´ 0,80 y una riqueza específica de 83; y los más bajos en la localidad VIII de La Auyama con un H´ de 2,17 bits/ind J` 0,95 y 10 especies. De los gasterópodos, 40,4% fueron carnívoros y el resto 59,6% herbívoros. En la clase Bivalvia, 49 especies (89,7%) fueron filtradores suspensívoros y solo 6 (11,3%) colectores de materia orgánica depositada. En general esta elevada riqueza es producto de la heterogeneidad de ambientes y sustratos, la elevada productividad de sus aguas y el bajo grado de intervención antrópica en la mayoría de sus localidades.The composition and distribution of littoral marine molluscs from northern Macanao Peninsula, Margarita’s Island are studied. The samples were collected in thirteen sites between February and May 2007. The captures were performed manually and with the aid of snorkeling. Overall, 2270 individuals were studied. They were distributed among 68 families, 106 genera and 152 species, belonging to Bivalvia Linnaeus, 1758, Gastropoda Cuvier, 1797 and Polyplacophora Gray, 1821 classes. The Gastropods were the dominant class with 89 species, being Lottia antillarum (Sowerby,1931) and Purpura patula (Linnaeus,1758 ) the two species with greater distribution, present in 84.6% of the samples. Among the 55 bivalve species identified the ones with higher distribution were Isognomom bicolor (C.B. Adams, 1845) present in 76.9% and Arca zebra in 69.2% of the samples. For the Polyplacophora, Chiton marmoratus Gmelin, 1791 and C. Tuberculatus Linne, 1758 were present in 69.2% of the samples and Acanthopleura granulata (Gmelin, 1791) in 53.8%. The highest value in diversity, evenness and richness were obtained at the locality VII of El Faro de Punta Tigre II with (H`) 3.80 bits/ind, (J`) 0.80 and specific richness of 83. The lowest values were found at the locality VIII of La Auyama with (H`) 2.2 bits/ind (J`) 0, 95 and 10 species. Among the gastropods, 40.4% were carnivores and the rest (59.6%) were herbivores. In the class Bivalvia, 49 species (89.7%) were suspension feeders and only 6 (11.3%) were surface deposit feeders. All the species of Polyplacophora were herbivores. In general, this high specific richness is the result of the heterogeneity of environments and substrates, the richness of macroalgae, the high productivity of the waters and the low degree of anthropic intervention in most of the sites.Fil: Capelo, Juan Carlos. Fundación La Salle de Ciencias Naturales; VenezuelaFil: Rada, Martin. Universidad de Oriente; VenezuelaFil: Sole, Maria. Fundación La Salle de Ciencias Naturales; VenezuelaFil: Buitrago, Joaquin. Fundación La Salle de Ciencias Naturales; VenezuelaFil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Narvaez, Jose. Fundación La Salle de Ciencias Naturales; Venezuel
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