136,372 research outputs found
Praeconium pro P.M. di Emilio Villa
No full textIl saggio – presentato al convegno dedicato a Piero Manzoni il 25 novembre 2014 – si concentra sui rapporti tra Piero Manzoni e l’ambiente romano e in particolare su quelli con il poeta e critico d’arte Emilio Villa. Uno scambio non sempre facile (fino alla rottura definitiva), ma molto significativo e denso.
Nel 1959 Piero Manzoni strinse rapporti con l’ambiente artistico e culturale romano, non solo attraverso la crescente collaborazione con il “Pensiero Nazionale” dell’amico Antonio Caputo, ma anche grazie alle esposizioni presso la Galleria Appia Antica, diretta da Villa. Del rapporto dei due vengono ricostruite e contestualizzate varie tappe: la mostra “Bonalumi, Castellani, Manzoni” tenutasi alla Galleria Appia Antica dal 3 al 15 aprile 1959 con una presentazione di Leo Paolazzi in catalogo, e la tappa italiana della mostra Zero, versante olandese, dal 24 ottobre al 4 novembre 1959; il fascicolo-libro donato da Manzoni a Villa, intitolato “PMP Piero Manzoni Pirla”, sempre del 1959; il testo dedicato a Manzoni, Castellani, Angeli, Festa, Schifano, Uncini e Lo Savio, pubblicato su “Aujourd’hui” nel settembre 1960; il lungo testo di Villa su Manzoni, scritto e rivisto a più fasi tra il 1960 e il 1961; la mostra “Castellani e Manzoni” alla Galleria La Tartaruga inaugurata il 22 aprile 1961, quando Manzoni firmò le proprie Sculture viventi, tra cui lo stesso Villa.
In particolare il saggio si sofferma e analizza da un punto di vista contenutistico, stilistico e linguistico il testo di Villa su Manzoni, proponendo una lettura filologica che identifichi e recuperi specifici riferimenti al lavoro dell’artista e definisca le caratteristiche e il livello di rielaborazione da parte di Villa, il quale non descrive o spiega le opere di un artista, ma le analizza e rielabora all’interno di un discorso verbale di grande sperimentazione, che offre aperture significative per la comprensione del lavoro di Manzoni.The essay - proposed to the conference Piero Manzoni on November 25, 2014 - focuses on the relationship between Piero Manzoni and the poet and art critic Emilio Villa; a relationship non really easy, but so important. Piero Manzoni established direct inks with artistic panorama in Rome in 1959: with his frined Antonio Caputo, art critics for "Piero Nazionale" journal, with Appia Antica Gallery, directed by Villa. The essay highlights the steps of the realtionship between Manzoni and Villa: the show "Bonalumin, Castellani, Mnazoni" held at Appia Antica Gallery from Aril 3 to 15 april (foreword in catalogue by Leo Paolazzi), exhibition of Zero Group (October 24-November 4, 1959), the handbook "PMP. Piero Manzoni Pirla" donated by Manzoni to Villain 1959; the Villa's essay dedicated to Manzoni, Castellani, Angeli, Festa, Unicini, and Lo Savio, published in "Aujourd'hui" art magazine on september 1960; a long essay on Manzoni wrote by Villa on 1960-61; the show "Castellani and Manzoni" held at La Tartaruga Gallery on April 22 1961, when Manzoni signed the Living Sculptures, among Villa. The essay analyses Villa's text on Manzoni, pointing out the specific features of Manzoni's art works and the linguistic and Villa's point of wiew
Synthesis of dual action Smac/Zinc-Chelator conjugates as putative proapoptotic agents
No full textApoptosis, or programmed cell death, is a critical cell process in normal development and homeostasis of multicellular organisms. It is now recognized that dysfunction of the apoptosis machinery is a hallmark of cancer. Accordingly, targeting critical apoptosis regulators is an attractive approach for the development of new classes of therapies for the treatment of cancer and other human diseases.
The X-linked inhibitor of apoptosis protein (XIAP) is a member of IAP proteins that potently inhibit apoptosis[1]. XIAP contains three baculovirus IAP repeat (BIR) domains. The mechanism of action of XIAP entails its binding with initiator and effector caspases through its BIR domains. In cells, the anti-apoptotic function of XIAP is antagonized by Smac/DIABLO (second mitochondria-derived activator of caspases or direct IAP binding protein with low pI). Despite the rather complex structure of Smac, its short N-terminal AVPI sequence is sufficient to trigger the inactivation of anti-apoptotic XIAP[2]. Our research group has shown how small monomeric, AVPI-inspired Smac mimics can bind XIAP on its BIR3 domain with sub-micromolar potency[3,4]. Executioner caspase-3, -6 and -7 exist within the cytosol as inactive zymogens (procaspases) activated by limited proteolysis within their inter-domain linker, carried out by an initiator caspaseThe essential executioner caspase-3 is proteolytically activated by either caspase-8 or -9. Zinc ions co-localize with procaspase-3/caspase-3 and inhibit its enzymatic activity in the cell by direct interaction with an Asp-Asp-Asp (DDD) “safety catch” region[5]. Thus, in this work we coupled a zinc chelator moiety based on di(picolylamide)amine (DPA) and its N,N-bis(pyridin-2-ylmethyl)ethane-1,2-diamine (BPEN) derivative to pro-apoptotic Smac mimetics, synthesized starting from known intermediates 1 and 2.
Dual action Smac mimetic-zinc chelators 3 and 4 were prepared from compounds 1 and 2 as shown in Scheme 1 and characterized in vitro, using cell-free and cellular assays[6,7]. Their ability to bind XIAP BIR3 domain, to process pro-caspase-3 to caspase-3 and their cytotoxicity have been experimentally determined, and favorably compared with those of a potent Smac mimic compound, especially for the most potent, tribasic dual action compound 4. Furthermore, the Zinc affinity for both compounds was confirmed by fluorescence measurements.
References
1. Q. L. Deveraux, J. C. Reed. Genes & Dev. 1999, 13, 239-252
2. J. Chai, C. Du, J. W. Wu, S. Kyin, X. Wang, Y.Shi. Nature 2000, 406, 855-862
3. P. Seneci, A. Bianchi, C. Battaglia, L. Belvisi, M. Bolognesi, A. Caprini, F. Cossu, E. de Franco, M. de, D.
Delia, C. Drago, A. Khaled, D. Lecis, L. Manzoni, M. Marizzoni, E. Mastrangelo, M. Milani,I. Motto, E.
Moroni, D. Potenza, V. Rizzo, F. Servida, E. Turlizzi, M. Varrone, F. Vasile, C. Scolastico. Bioorg. Med.
Chem. 2009, 17, 5834–5856.
4. L. Manzoni, D. Arosio, L. Belvisi, A. Bracci, M. Colombo, D. Invernizzi, C. Scolastico. J. Org. Chem. 2005, 70,
4124-4132.
5. K. S. Putt, G. W. Chen, J. M. Pearson, J. S Sandhorst, M. S. Hoagland, J. Kwon, S. Hwang, H. Jin, M. I.
Churchwell, M. Cho, D. R. Doerge, W. G. Helferich, P. J. Hergenrother. Nat. Chem. Biol. 2006, 2, 543, 550
6. Z. Nikolovska-Coleska, R. Wang, X. Fang, H. Pan, Y. Tomita, P. Li, P.P. Roller, K. Krajewski, N.G. Saito, J.A.
Stuckey, S. Wang, Anal. Biochem. 2004, 332, 261-273.
7. Z. Nikolovska-Coleska, J.L. Meagher, S. Jiang, S.A. Kawamoto, W. Gao, H. Yi, D. Qin, P.P. Roller, J.A.
Stuckey, S. Wang, Anal. Biochem. 2008, 374, 87-98
Phenomenological Method in Psychology: The Problem of Existence and the Phenomenology of Mood
No full textFenomenološka metoda v psihologiji se nanaša na določen pristop k psihološkemu razmišljanju, raziskovanju in praksi, ki črpa možnosti načina raziskovanja in teorijo iz kulturnih ved oz. humanistike. Takšna usmeritev temelji na fenomenologiji, filozofiji eksistencializma, hermenevtiki ter (socialnemu) konstrukcionizmu. Osrednji poudarek je na razumevanju ter osmišljanju temeljnih struktur zavesti in »življenjskega sveta« ter žive in celostne izkušnje subjekta raziskave ali pa klienta, ki je v zdravniški obravnavi. Razumevanje življenjskega sveta kot področja, kjer se rodi neka specifična psiho(pato)logija, s katero posameznik v svetu biva, nam ponudi konceptualno orodje za raziskovanje doživljanja v psihologiji. Takšne vrste metoda sistematičnega raziskovanja duševnosti (ki ni introspektivno), zaobjema konkretno izkušnjo vedno že utelešenega in situiranega subjekta v (zgodovinsko-kulturnem) svetu. Subjekt in svet nista ločeni entiteti, temveč sovpadata tako, da vedno že tvorita enotnost. Svet kot svet pa nam je dan le prek razpoloženja, in sicer skozi temeljno počutje. Fenomenološko preučevanje razpoloženjskih in drugih afektivnih stanj lahko tako signifikantno vpliva na razumevanje številnih psihiatričnih bolezni, saj nam razpoloženja primarno strukturirajo celotno bivanjsko resničnost. Ker pa se vsako raziskovanje zavesti začne s percepcijo – saj je neko védenje vedno že vzpostavljeno v področju in horizontih, ki jih odpira naša percepcija –, mora biti tudi v raziskovanje afektivnih stanj vključeno fenomenološko razumevanje percepcije in telesa.The phenomenological method in psychology refers to a particular approach to thinking, research, and practice in psychology, drawing on possibilities in the ways of inquiry and theory used in cultural studies and humanities. This orientation is based on phenomenology, the philosophy of existentialism, hermeneutics and (social) constructionism. The main focus is on understanding and conceptualizing the fundamental structures of consciousness – of the “lifeworld” – and the live and holistic experience of the research subject or client undergoing medical treatment. Understanding the lifeworld as an area where every psycho(patho)logy emerges with which one exists in the world, offers us a conceptual tool for exploring experiences in psychology. This kind of method of systematic research of psyche (which is not introspective) captures the concrete experience of an always embodied and situated subject in the (specific socio-historical) world. The subject and the world are not separate entities but coincide in that they always form a unity. The world as a world, however, is given to us only through (fundamental) mood. Phenomenological study of mood and other affective states can thus significantly contribute to understanding many psychiatric disorders, since moods primarily structure our entire (existential) reality. However, since every study of consciousness begins with perception – as knowledge in this field and horizons which our perception opens to us already exists – so must the phenomenological understanding of perception and the body be included in the research of affective states
Psychology and religion: the depth psychology and the phenomenology of existential feelings
No full textI CARATTERI SENSORIALI DEL MANZONI BIANCO
No full textRicerca dei descritttori del Manzoni bianco utilizzando 29 vini , addestramento dei giudici per definire il profilo sensoriale
Prophylactic Total Gastrectomy: Techniques
No full textProphylactic total gastrectomy (PTG) represents the treatment of choice for carriers of pathogenetic CDH1 mutations in the context of hereditary diffuse gastric cancer (HDGC) syndrome. PTG consists in a total gastrectomy: it could be performed both through open or minimally invasive surgery depending on the surgeon’s expertise, but the higher rate of postoperative complications reported after laparoscopic procedures should be taken into account especially in the specific context of a prophylactic surgery. The intra-operative control of resection margins with frozen section is mandatory to exclude residual of gastric mucosa. D1/D1+ is suggested but D2 dissection can be considered. Roux-en-Y reconstruction is preferred. Long-term follow-up shows a good quality of life after this type of surgery despite the weight loss
Ester fra tragedia e oratorio nella drammaturgia di Francesca Manzoni
No full textIl saggio ricostruisce affinità compositive e differenze nella scrittura drammaturgia di Francesca Manzoni relativa al personaggio di Este
Ester fra tragedia e oratorio nella drammaturgia di Francesca Manzoni
No full textIl saggio analizza la ricorrenza e trattazione del tema biblico della regina Ester nella drammaturgia della poetessa Francesca Manzoni
Il federalismo alla prova (Rosmini, Manzoni)
No full textLa proposta per un assetto federale concreto per l'Italia che provenne da Antonio Rosmini merita di essere studiata e capita. L'ideale federalistico rosminiano fu avversato dal suo grande amico, Alessandro Manzoni, il quale non riteneva possibile una coabitazione in Italia di tanti Principi sotto un'autorità federale. Manzoni non riteneva possibile affidare al papa Pio IX la presidenza della Confederazione italiana allo scopo di modificare l'assetto dello stesso potere temporale del papa
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