1,720,977 research outputs found
Resveratrol-poor red wines modulate SIRT1 in human renal cells.
No full textThe cardioprotective and anti-aging effects of red wine phenols, especially resveratrol (RSV), are well known. One of the most interesting biological properties of RSV and other naturally occurring phenols is the regulation of the expression and activity of SIRT1 (silent mating type information regulation 2 homolog). In view of the role of SIRT1 in acute and chronic renal diseases, we decided to study the effects of RSV-poor red wines on the expression of SIRT1 and HIF-2α (hypoxia-inducible factor 2α) to be compared with a nanomolar concentration of RSV or malvidin in proximal tubular cells of human kidneys (PTEC). Survival signaling systems activation (extracellular signal-regulated kinases, ERK and AMP-activated protein kinase, AMPK) was also investigated in PTEC incubated with wines. PTEC cells were incubated in the presence of RSV-poor wines diluted 1:1,000 for 30', 90', 120' and 24 h. Expression of SIRT1 and HIF-2α, and activation of ERK and AMPK were analyzed by Western Blot. The data obtained show that wine modulates the expression of anti-aging molecular systems even when RSV is present in very small amounts
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Sucralfate modulates uPAR and EGFR expression in an experimental rat model of cervicitis
No full textTreatment with 1,25-dihydroxyvitamin D3 preserves glomerular slit diaphragm-associated protein expression in experimental glomerulonephritis
No full textIn this study, we investigated the effect of 1,25(OH)(2)D-3 on proteinuria and on the alteration of slit diaphragm-associated proteins induced by anti-Thy 1.1 in Wistar rats. Four groups of animals were studied: group I, anti-Thy 1.1 treated rats; group II, anti-Thy1.1 treated group that at day 2, after the onset of overt proteinuria, started the treatment with 1,25(OH)(2)D-3; group III, normal control rats injected with vehicle alone; group IV, rats that received only 1,25(OH)(2)D-3. At day 2, in group I and II, before the administration of 1,25(OH)(2)D-3 protein excretion was significantly increased when compared to controls. Overt proteinuria was maintained until day 14 in group I whereas in group II protein excretion was significantly reduced from day 3 to day 14. Moreover, treatment with 1,25(OH)(2)D-3 abrogated podocytes injury, detected as desmin expression and loss of nephrin and zonula occludens-1 (ZO-1), two slit diaphragm-associated proteins, and glomerular polyanion staining, that were observed in group I. In conclusion, these results suggest that 1,25(OH)(2)D-3 administrated with a therapeutic regiment may revert proteinuria, counteracting glomerular podocyte injury
The influence of hemodynamic stress factors, VEGF and il 12 Plasma and CSF levels and circle of willis congenital abnormalities on intracranial aneurysm formation
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Attività antiradicalica e antiossidante dei vini rossi italiani
No full textL'attività antiossidante non è stata del tutto chiarita per molti vini e solo pochi studi hanno tentato di
individuare la relazione tra la composizione chimica del vino e la sua attività biologica. Inoltre vini
differenti hanno poche somiglianze chimiche e un differente contenuto in polifenoli, quindi questo
può essere associato con la discrepanza di risultati delle precedenti analisi.
In questo studio abbiamo indagato l'attività antiossidante e antiradicalica di alcuni vini rossi italiani
e dei componenti isolati per comprendere il meccanismo di azione dei complessi polifenolici del
vino rosso. Abbiamo eseguito delle analisi chimiche dei vini per determinare il contenuto totale di
polifenoli, antocianine, resveratrolo e catechine totali. I risultati mostrano che, nei vini rossi testati,
il range dei polifenoli totali varia da 1117 mg/L a 2821 mg/L, le antocianine da 46 mg/L a 178
mg/L, il resveratrolo da tracce a 12 mg/L e le catechine totali da < 50 mg/L a >1400 mg/L.
Per studiare l'attività antiradicalica e antiossidante abbiamo utilizzato metodi in vitro come il
DPPH* e l'elettrochimica.
Il test del DPPH* mostra che i vini con il più alto contenuto in polifenoli totali e antocianine sono i
più attivi e l'acido gallico, la quercitina, l'(-)-epicatechina e la malvidina hanno IC50 più basso di 10
mg/mL.
La voltammetria ciclica è un metodo utile per confermare che le antocianine hanno un potenziale di
ossidazione molto basso. I vini rossi mostrano un'ossidazione irreversibile a 420-460 mV.Abbiamo eseguito anche un test in vivo per valutare la protezione di cellule HeLa incubate con
perossido di idrogeno.In conclusione i polifenoli del vino rosso giocano un ruolo importante come antiossidanti e i
composti maggiormente distinguibili, come le antocianine e il resveratrolo, sono fondamentali
nell'esercitare effetti biologici. Buoni risultati sono stati ottenuti anche a concentrazioni molto basse
di tutti i campioni e dei composti isolati, escludendo l'effetto negativo dell'etanolo e sorpassando il
problema rappresentato dalla scarsa biodisponibilità dei polifenoli
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