1,720,969 research outputs found
Estabilidade de cortes de lagarto bovino (M. semitendinosus) injetados e assados contendo diferentes sais de sodio e extrato de alecrim
Get PDFOrientador: Marise Aparecida Rodrigues PollonioTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de AlimentosDoutoradoDoutor em Tecnologia de Alimento
Desempenho da metodologia para isolomento e contagem de Escherichia coli 0157:H7 em leite e queijo e sua ocorrencia em queijo minas frescal produzido comercialmente
No full textOrientador: Mauro Faber de Freitas LeitãoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de AlimentosResumo: A etapa inicial deste trabalho consistiu na avaliação da resistência de uma cepa de Escherichia colí 0157:H7 e da microbiota contaminante de queijo Minas frescaI a antibióticos utilizados para conferir seletividade a meios de isolamento dessa bactéria. Observou-se que E colí 0157:H7 mostrou sensibilidade a concentrações de novobiocina superiores a 30 mg/L, sendo desaconselhável o uso de 100 mg/L, conforme preconizado em alguns meios de isolamento. Como o microrganismo mostrou-se menos tolerante à combinação de três antibióticos (cefixima a 0,05 mg/L, cefsuloqina a 10 mg/L e vancomicina a 8 mg/L) do que a microbiota contaminante do queijo frescal, tornou-se questionável a indicação do
uso desses antibióticos para conferir seletividade aos meios de isolamento da bactéria. Em outra etapa foram avaliados os meios seletivos MacConkey sorbitol modificado, HC de acordo com 8zabo et aI. (1986), Rainbow 0157,
EMB modificado e 8039 modificado, suplementados ou não com cefixima (0,05 mg/L) e telurito de potássio (2,5 mg/L), na contagem de Ecolí 0157:H7, inoculada experimentalmente em amostras de queijo Minas frescal e de leite, submetidas ou não a tratamento térmico. Os resultados revelaram a inadequação de todos esses meios nas contagens de amostras apresentando elevada contaminação (sem tratamento térmico). Nas amostras com contaminação baixa (tratadas termicamente), os meios HC e 8039 modificado, ambos suplementados, revelaram desempenho superior na análise de queijo (95,9 e 95,0 % de recuperação, respectivamente). Na análise de leite, o HC foi o mais eficiente, levando a 85,3% de recuperação. A técnica do Número Mais Provável revelou-se deficiente e pouco prática na contagem de E colí 0157:H7 em amostras de queijo, não representando, portanto, uma alternativa recomendável. Na avaliação qualitativa de Ecolí 0157:H7 foram avaliados os enriquecimentos em caldo BPW + 3 antibióticos, segundo Blanco et aI. (1996), em meio mEC+n segundo
Okrend & Rose (1989), em meio mT8B segundo Padhye & Ooyle (1991), além do método proposto pelo FOA (1998) e o método imunoenzimático TECRA@. Na análise de amostras de queijo Minas frescal inoculadas experimentalmente, nenhum dos sistemas revelou desempenho altamente satisfatório. No entanto, entre as metodologias clássicas testadas, a do FDA mostrou-se superior, enquanto o método TECRA@foi aquele que, comparativamente, possibilitou a maior porcentagem de isolamentos e menor índice de resultados falso-negativos. Na análise de 60 amostras de queijo Minas frescal, submetidas ou não a inspeção federal, nenhuma delas revelou a presença de E. coli 0157:H7. Os resultados obtidos indicaram a necessidade de se aperfeiç.oar a metodologia para a pesquisa
deste patógeno em alimentos, principalmente naqueles cuja microbiota
contaminante apresenta-se em números elevadosAbstract: In a first experiment, it was evaluated the tolerance of Escheríchía colí 0157:H7 and natural contaminants found in Minas frescaI cheese to antibiotics used in selective culture media. It was noticed that E. colí 0157:H7 exhibited low tolerance to novobiocin concentrations above 30 mg/L, although the use of 100 mg/L is indicated in some isolation media. The microorganism was less tolerant to antibiotic combination (cefixime at 0,05 mg/L, cefsulodin at 1O mg/L and vancomincin at 8 mg/L) when compared to the natural contaminant cheese microflora what could be a drawback concerning the use of these agents in selective culture media. In a second experiment, the selective media MacConkey sorbitol (modified), HC according to Szabo (1990), Rainbow 0157, modified EMB and modified S039, supplemented or not with cefixime (0,05 mg/L) and potassium
telurite (2,5 mg/L) were evaluated for E. colí 0157:H7 counting, with the bacterium inoculated in Minas frescal cheese and milk samples, both submitted or not to thermal treatment. Ali the tested media were inadequate when used for bacterial counts in the samples with high natural contamination (without thermal treatment). However, when the cheese samples with lower contamination were analyzed, the media HC and modified S039, both supplemented, showed superior performance
(95,9 and 95,0% recovery, respectively). In milk analysis, HC medium was the most efficient (85,3% recovery). The Most Probable Number technique showed an inadequate performance for E. colí 0157:H7 counts. For the qualitative evaluation of E. coli 0157:H7 it was evaluated BPW+3 antibiotics broth according to Blanco et a/. (1996), mEC+n (Okrend & Rose, 1989), mTSB (Padhye & Ooyle, 1991), FOA proposed method (1998) and the immunoenzimatic method [email protected] of the tested methods showed superior performance for E. calí 0157:H7 recovery in inoculated cheese samples in the presence of the natural microflora. However the
FDA method was the most efficient among the classical ones, while the TECRA@ assay was the best concerning E. calí 0157:H7 recovery and no false negative results. The results suggest the need for improvment in the methodology for E. calíMestradoMestre em Tecnologia de Alimento
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
- …
