77 research outputs found
“The colour of words. An analysis of the Gospel of John. From ‘social death’ to freedom, in the household”
No full textIn the text of John, the deep social reality of slavery, especially of the domestic one, comes to the surface. It is a widespread and influential reality that the Gospel of John seems to know very well. Its terminology demonstrates a knowledge of the actual functions of the slaves and of the forms of relationships that bind them to their masters. The author shows capacity in the symbolic use of the slave terminology and is able to adapt it to his project
Identification and functional characterization of malignant hyperthermia mutation T1354S in the outer pore of the Cav alpha1S-subunit
No full textTo identify the genetic locus responsible for malignant hyperthermia susceptibility (MHS) in an Italian family, we performed linkage analysis to recognized MHS loci. All MHS individuals showed cosegregation of informative markers close to the voltage-dependent Ca2+ channel (CaV) alpha1S-subunit gene (CACNA1S) with logarithm of odds (LOD)-score values that matched or approached the maximal possible value for this family. This is particularly interesting, because so far MHS was mapped to >178 different positions on the ryanodine receptor (RYR1) gene but only to two on CACNA1S. Sequence analysis of CACNA1S revealed a c.4060A>T transversion resulting in amino acid exchange T1354S in the IVS5-S6 extracellular pore-loop region of CaV alpha 1S in all MHS
subjects of the family but not in 268 control subjects. To investigate the impact of mutation T1354S on the assembly and function of the excitation-contraction coupling apparatus, we expressed GFP-tagged alpha1ST1354S in dysgenic (alpha1S-null) myotubes. Whole cell patch-clamp analysis revealed that alpha1ST1354S produced significantly faster activation of L-type Ca2+ currents upon 200-ms depolarizing test pulses compared with wild-type GFP-alpha1S (alpha1SWT). In addition, alpha1ST1354Sexpressing
myotubes showed a tendency to increased sensitivity for caffeine-induced Ca2+ release and to larger action-potential-induced
intracellular Ca2+ transients under low (< 2 mM) caffeine concentrations compared with alpha1SWT. Thus our data suggest that an additional influx of Ca2+ due to faster activation of the alpha1ST1354S L-type Ca2+ current, in concert with higher caffeine sensitivity of Ca2+ release, leads to elevated muscle contraction under pharmacological trigger, which might be sufficient to explain the MHS phenotype
Dual Current: Inseparable Elements in Painting and Architecture (Exhibition Catalogue)
No full textDual Current: Inseparable Elements in Painting and Architecture, curated by Gabriele Evertz, examines the relationship between painting and architecture in a contemporary context through color, shape, and theory.
The artists whose works are featured in this exhibition are: Josef Albers, Matthew Deleget , Peter Dudek, Cris Gianakos, Michelle Grabner, Lynne Harlow, Changha Hwang, Russell Maltz, Rossana Martinez, Kristine Marx, and Manfred Mohr. Their works link three-dimensional space and the picture plane to create radical new forms. Dual Current explores the relationship between painting and architecture, closely intertwined since the Renaissance
The distal C terminus of the dihydropyridine receptor β(1a) subunit is essential for tetrad formation in skeletal muscle
No full textThe skeletal muscle dihydropyridine receptor (DHPR) β(1a) subunit is indispensable for full trafficking of DHPRs into triadic junctions (i.e., the close apposition of transverse tubules and sarcoplasmic reticulum [SR]), facilitation of DHPRα(1S) voltage sensing, and arrangement of DHPRs into tetrads as a consequence of their interaction with ryanodine receptor (RyR1) homotetramers. These three features are obligatory for skeletal muscle excitation–contraction (EC) coupling. Previously, we showed that all four vertebrate β isoforms (β(1)–β(4)) facilitate α(1S) triad targeting and, except for β(3), fully enable DHPRα(1S) voltage sensing [Dayal et al., Proc. Natl. Acad. Sci. U.S.A. 110, 7488–7493 (2013)]. Consequently, β(3) failed to restore EC coupling despite the fact that both β(3) and β(1a) restore tetrads. Thus, all β-subunits are able to restore triad targeting, but only β(1a) restores both tetrads and proper DHPR–RyR1 coupling [Dayal et al., Proc. Natl. Acad. Sci. U.S.A. 110, 7488–7493 (2013)]. To investigate the molecular region(s) of β(1a) responsible for the tetradic arrangement of DHPRs and thus DHPR–RyR1 coupling, we expressed loss- and gain-of-function chimeras between β(1a) and β(4), with systematically swapped domains in zebrafish strain relaxed (β(1)-null) for patch clamp, cytoplasmic Ca(2+) transients, motility, and freeze-fracture electron microscopy. β(1a)/β(4) chimeras with either N terminus, SH3, HOOK, or GK domain derived from β(4) showed complete restoration of SR Ca(2+) release. However, chimera β(1a)/β(4)(C) with β(4) C terminus produced significantly reduced cytoplasmic Ca(2+) transients. Conversely, gain-of-function chimera β(4)/β(1a)(C) with β(1a) C terminus completely restored cytoplasmic Ca(2+) transients, DHPR tetrads, and motility. Furthermore, we found that the nonconserved, distal C terminus of β(1a) plays a pivotal role in reconstitution of DHPR tetrads and thus allosteric DHPR–RyR1 interaction, essential for skeletal muscle EC coupling
Author Correction: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
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Patterns of primes in arithmetic progressions
No full textAfter the proof of Zhang about the existence of infinitely many bounded gaps between consecutive primes the author showed the existence of a bounded d such that there are arbitrarily long arithmetic progressions of primes with the property that p′ = p + d is the prime following p for each element of the progression. This was a common generalization of the results of Zhang and Green-Tao. In the present work it is shown that for every m we have a bounded m-tuple of primes such that this configuration (i.e. the integer translates of this m-tuple) appear as arbitrarily long arithmetic progressions in the sequence of all primes. In fact we show that this is true for a positive proportion of all m-tuples. This is a common generalization of the celebrated works of Green-Tao and Maynard/Tao.
Dedicated to the 60th birthday of Robert F. Tich
Regions of the DHPRβ1A Subunit Responsible for DHPR Voltage-Sensing in Skeletal Muscle Excitation-Contraction Coupling
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