306,797 research outputs found
Intervista a Dario Maccarrone
No full textIl contributo riporta gli esiti dell'intervista effettuata a Dario Maccarrone, curata dall'autore dell'articolo, in riferimento al ruolo delle startup nei territori interni
Duchesne e La Curia Romana
No full textMaccarrone Michèle. Duchesne e La Curia Romana. In: Mgr Duchesne et son temps. Rome : École Française de Rome, 1975. pp. 401-494. (Publications de l'École française de Rome, 23
MODULATION OF THE ENZYMATIC ACTIVITY AND MEMBRANE BINDING PROPERTIES OF SOYBEAN LIPOXYGENASE-1 THROUGH LIMITED PROTEOLYSIS AND METAL SUBSTITUTION
No full textLipoxygenases are non-heme, non-sulfur iron containing enzymes that catalyze the dioxygenation of polyunsatured fatty acids containing one or more pentadiene systems to the corresponding hydroperoxy derivatives. Structural studies in solution of the mammalian and plant enzyme revealed that the latter has a more stable and compact conformation1. As yet, metal atom extraction, reconstitution and substitution with vicariate metals have not been successfully applied to soybean lipoxygenase-1, because of the highly buried position of the iron atom within the active site. Tryptic digestion of lipoxygenase-1 and the subsequent isolation of the 60 kDa C-terminal region allowed to generate a “mini-lipoxygenase-1 (miniLOX)” that retains the catalytically active iron, but in a more accessible position2. In this study, we investigated by near-UV-circular dichroism and fluorescence spectroscopies the structural and functional effects of iron removal, reconstitution and vicariation in miniLOX. Moreover, we report the kinetic analysis and the membrane binding ability of the apo- and metal-substituted forms of miniLOX, using fluorescence resonance energy transfer and monolamellar vesicles. Taken together, these data demonstrate an unprecedented structural role of iron, which is involved not only in the catalytic activity but also in the membrane binding ability of lipoxygenase-1.1. Dainese E. Sabatucci A. van Zadelhoff G. Angelucci C. B. Vachette P., Veldink G. Finazzi Agrò A. and Maccarrone M. (2005). J. Mol. Biol. 349, 143-152.2. Maccarrone M. Salucci M. L. van Zadelhoff G. Malatesta F. Veldink, G. Vliegenthart J. F. and Finazzi Agrò A. (2001). Biochemistry 40, 6819-6827.[...
Natural Endocannabinoid Derivatives as Templates for the Development of FAAH Inhibitors
No full textThe endogenous cannabinoids (endocannabinoids) are amides, esters and ethers of long chainpolyunsaturated fatty acids. These lipids are bioactive signaling molecules that show diverse cellular andphysiological effects and play various roles both in the central nervous system and in the periphery. Thediscovery of N-arachidonoylethanolamine (anandamide, AEA) and of the enzyme that terminates its signaling,i.e. fatty acid amide hydrolase (FAAH), have inspired pharmacological strategies to augment endocannabinoidtone and biological activity through inhibition of FAAH. Here we discuss the role of natural endocannabinoidderivatives, like the hydroxy-anandamides (OH-AEAs) generated from AEA via lipoxygenase activity, aspowerful inhibitors of FAAH. We propose that these compounds, by reversibly inhibiting FAAH, may controlin vivo the endocannabinoid tone. We discuss also the potential value of OH-AEAs as templates for thedevelopment of next-generation therapeutics that act at specific sites of FAAH.[...
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
TRA ARTE E ATTIVISMO, ISTRUZIONI PER L\u27USO 1.0. Catalogo della mostra (Pisa, 2011): a cura di S. Vassallo e F. Maccarrone
No full textGiacomo Verde - Tra arte e attivismo. Istruzioni per l\u27uso 1.0
Catalogo della mostra a cura di Silvana Vassallo e Francesca Maccarrone (Pisa, Studio Gennai, 2011)
 
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