980 research outputs found
The Role of Anisakis sp. in α-Gal Sensitization: Implications for Parasitic-Induced Meat Allergy
No full textAuthor Contributions:
Conceptualization, M.R., A.S.O., F.V., A.D. and C.C.; methodology, M.R., S.R., Á.V. and J.G.-F.; validation, M.R., J.G.-F., A.D. and C.C.; formal analysis, M.R., J.G.-F., A.D. and C.C.; investigation, M.R., S.R., Á.V., J.G.-F., A.S.O., F.V., A.D. and C.C.; resources, A.S.O., F.V. and C.C.; data curation, M.R., J.G.-F., A.D. and C.C.; writing—original draft preparation, M.R. and C.C.; writing—review and editing, M.R., J.G.-F., A.D. and C.C.; funding acquisition, A.S.O., F.V. and C.C. All authors have read and agreed to the published version of the manuscriptBackground/Objectives:
This study investigates the potential of Anisakis sp. as a novel source of α-Gal (Galα1-3Galβ1-4GlcNAc-R) epitopes capable of inducing allergic sensitization in humans. While α-Gal is classically associated with delayed IgE-mediated hypersensitivity following tick bites, emerging evidence suggests that parasitic helminths such as Anisakis sp. may also express α-Gal-containing glycoconjugates, offering an alternative sensitization pathway.
Methods:
Protein extracts from Anisakis sp. third-stage larvae and mammalian tissues (beef, pork) were analyzed by SDS-PAGE and Western blot using a monoclonal anti-α-Gal antibody (clone M86), and α-Gal epitopes were detected by ELISA. Sera from urticaria patients, stratified by Anisakis sp. sensitization status, were evaluated for anti-α-Gal IgG, IgE, and IgG4 antibodies. Inhibition assays assessed cross-reactivity
Results:
Results confirmed the presence of α-Gal epitopes on Anisakis sp. proteins, with prominent bands at ~250 kDa and 65 kDa. Urticaria patients sensitized to Anisakis sp. exhibited significantly elevated anti-α-Gal antibody levels compared to controls. Inhibition ELISA demonstrated substantial reduction in antibody binding with Anisakis sp. extracts, indicating shared antigenic determinants with mammalian α-Gal.
Conclusions:
These findings establish Anisakis sp. as a source of α-Gal-containing glycoproteins capable of eliciting specific antibody responses in humans, highlighting a potential parasitic route for α-Gal sensitizationDepto. de Microbiología y ParasitologíaDepto. de Sanidad AnimalFac. de FarmaciaFac. de VeterinariaTRUEpu
Genetic Algorithm-Driven Protein Engineering: Enhancing PT8 Poly morphic Toxins for Biotechnology
No full text"Polymorphic toxins, such as PT8 from the Leptospiraceae family, play a significant role in mi crobial competition and have potential applications in biotechnology. However, their toxicity
often limits their utility in practical applications. This thesis presents a novel approach to opti mizing the PT8 polymorphic toxin for biotechnological use through the use of genetic algo rithms (GAs) and machine learning models to reduce its inherent toxicity while maintaining
functional integrity.
In this study, we first analyzed the structure and dynamics of PT8 using state-of-the-art protein
structure prediction tools like AlphaFold, RosettaFold, and ESMFold, coupled with molecular
dynamics simulations. These analyses revealed several key regions within PT8 that contribute
to its stability and function but also underlie its toxicity(...)"N/
The Gal family
No full textKnjiga "Porodica Gal" Magde Simin predstavlja dirljivu i dokumentaristički utemeljenu pripovest o jevrejskoj porodici iz Vojvodine, čija sudbina osvetljava širi kontekst stradanja, opstanka i identiteta tokom burnih istorijskih previranja 20. veka. Kroz priču o članovima porodice Gal, autorka pažljivo rekonstruiše svakodnevni život, porodične veze, kulturne običaje i tragediju Holokausta, oslanjajući se na arhivsku građu, fotografije, svedočanstva i lična sećanja. Ovo delo je i omaž izgubljenim životima, ali i čin sećanja koji doprinosi kulturi pamćenja i očuvanju jevrejskog nasleđa u Srbiji. "Porodica Gal" je važan doprinos lokalnoj istoriji, ali i univerzalna priča o ljubavi, gubitku i ljudskoj istrajnosti."The Gal Family" by Magda Simin is a touching, documentary-style narrative about a Jewish family from Vojvodina, reflecting the broader themes of suffering, survival, and identity amid the turbulent events of the 20th century. Through the story of the Gal family members, the author carefully reconstructs their daily lives, family bonds, cultural customs, and the tragedy of the Holocaust, drawing on archival materials, photographs, testimonies, and personal memories. This work serves as both a tribute to those who were lost and an act of remembrance that contributes to the culture of memory and the preservation of Jewish heritage in Serbia. "The Gal Family" makes a significant contribution to local history and remains a universal story of love, loss, and human resilience.Biblioteka Dokument
Comparative analysis of geographical names in the slovenian translations of j. r. r. tolkien`s the lord of the rings
No full textTema magistrske naloge je prevajanje in primerjava prevodov zemljepisnih imen, ki so vključena v fantazijsko delo Gospodar prstanov avtorja J. R. R. Tolkiena, ki je v pomoč prevajalcem izdal tudi navodila o prevajanju imen v Gospodarju prstanov. Roman so leta 1995 prvič prevedli Polona Mertelj, Primož Pečovnik in Zoran Obradović, drugi prevod pa je med letoma 2002 in 2004 opravil Branko Gradišnik. V sklopu analize naloga obravnava še teorijo posodabljanja prevodov. V romanu so prisotna številna edinstvena imena, ki na zanimiv in pretkan način opisujejo značilnosti različnih krajev. Velikokrat vključujejo tudi prvine staroangleškega in nordijskega besedišča ter so ukoreninjena z zgodovinskimi dejstvi iz romana. Zaradi tega so v prevodih uporabljene različne prevajalske strategije. Da bi bralci razumeli pomen in kulturno zasidranost teh imen, so v prevodih prisotne podomačitve in prilagoditve izvirnika ciljnemu jeziku. Primerjalna analiza izvirnika in obeh prevodov je pokazala, da so imena v prvotnem prevodu bolj podomačena kot v novejšem prevodu. Obenem pa je razvidno, da je prevajalec novejšega prevoda bolj dosledno upošteval Tolkienova navodila, kot pa so to storili prevajalci prvotnega prevoda.The thesis focuses on translations of geographical names in The Lord of the Rings, a fantasy work by J. R. R. Tolkien. The novel includes many unique names which describe the features of different landscapes in an interesting and exuberant manner. The names often include elements from the Old English and Nordic vocabulary and are rooted in the historical events of the novel. It is particularly interesting that to guide future translators the author published an essay Guide to the Names in The Lord of the Rings, in which he gave instructions on how to properly translate the names in his works. The novel was first translated into Slovene in 1995 by Polona Mertelj, Primož Pečovnik in Zoran Obradović. The novel was retranslated by Branko Gradišnik between 2002 and 2004. The thesis is based on the hypothesis of retranslation which claims that the initial translation is more domesticated and source-oriented than the subsequent. As we discovered, the translators often used the strategies of adaptation and substitution to help the readers understand all cultural connotations attached to the original meaning. Comparative analysis of the source text and both translations showed that in the first translation the names were more often translated by adaptation than in the second translation. On the other hand, the results of the research demonstrated that Gradišnik followed Tolkien\u27s rules more precisely than the translators of the first translation
I frutti dello Spirito Santo - il commento di san Tommaso d\u27Aquino ai Gal 5,22ss
No full textIn the presentation of the subject – preceded by an Introduction, where the Aquinas is presented as Bible’s Commentator – the author examines the saint Thomas’ interpretations of saint Paul’s texts regarding the theme of this research, confronted with the contemporary exegesis, and presents three points: 1 – The vices (Gal 5,19-21); 2 – The fruits of the Holy Spirit (Gal 5,22-23a); 3 – Against such there is no Law (Gal 5,23b; whit the interpretation of Gal 5,24-25). All this permits to the author to conclude that the doctrine of the fruits of the Holy Spirit presented by Saint Thomas in this Commentary is very clear, rich and remains valid and topical and permits to remain in the faith, morality and spirituality of the Church.In the presentation of the subject – preceded by an Introduction, where the Aquinas is presented as Bible’s Commentator – the author examines the saint Thomas’ interpretations of saint Paul’s texts regarding the theme of this research, confronted with the contemporary exegesis, and presents three points: 1 – The vices (Gal 5,19-21); 2 – The fruits of the Holy Spirit (Gal 5,22-23a); 3 – Against such there is no Law (Gal 5,23b; whit the interpretation of Gal 5,24-25). All this permits to the author to conclude that the doctrine of the fruits of the Holy Spirit presented by Saint Thomas in this Commentary is very clear, rich and remains valid and topical and permits to remain in the faith, morality and spirituality of the Church
Pulsating catalytic combustion of gaseous fuels
No full textIssued as First quarterly report, Semi annual report, and Final report, Project no. E-16-618Final report has title: Pulsating catalytic combustion of gaseous fuelsFinal report has co-author: Reuven Gal-E
Small animal models of Gal-mediated and xenograft rejection
No full textDeposited with permission of the author. © 2004 Dr. Hilton GockXenotransplantation is the final frontier of using vascularised organs or cellular grafts to treat end-organ disease and offers a potential solution to the worldwide shortage of human tissue available for transplantation. The main immunological barrier to xenografting from pig-to-primate is the antigen, Galactose-α1,3-Galactose (Gal) which is found in all species except humans and other higher primates. Even with the major advancement of deleting Gal from the potential pig donor species with the aid of cloning technology, complete elimination may be elusive as alternative genes yet to be fully characterised, may still produce Gal at low levels. Thus, the human immune response against Gal may continue to be a barrier to successful xenotransplantation. The aim of this project was to develop small animal models of the important components of xenograft rejection that largely relate to the anti-Gal immune response. These include models of hyperacute, acute vascular and chronic xenograft-like rejection that in turn, provide new insights in the immune mechanisms of the rejection processes. The role of antibody and both innate and cognate cellular immunity are explored. Both vascularised heart grafts and non-vascularised skin graft models are examined as rejection of solid organs may differ from cellular transplantation. The project also provides a platform for future studies in testing genetic and pharmacotherapeutic strategies to overcome the rejection processes uncovered
Rendezvous on an interval and a search game on a star
No full textRendezvous problems and search games (with two players) are related problems. In a rendezvous problem the players like to meet as soon as possible, while in a search game one of them tries to avoid the meeting as long as possible. So in a rendezvous problem the players have a common interest and in a search game the players have conflicting interests. We consider in this thesis a rendezvous problem on a discrete labeled interval and a search game on a star with players having motion detection abilities. This thesis consists of two parts. The first part (Chapter 2) is about the rendezvous problem on a discrete labeled interval. This problem was first introduced by Alpern in [1]. Most results for this problem are summarized in [2], Chapter 13. This thesis contains new results extending the results in [2]. The work was done in Delft in February 2008 until May 2008 and in July 2008. Moreover a part of the results in Chapter 2 were presented at the 13th International Symposium on Dynamics Games and Applications in Wroclaw, Poland. The second part of this thesis (Chapter 3) is about a search game on a star with players having motion detection abilities. This problem was proposed by Prof. S. Gal (University of Haifa, Israel) and until now no results were available. The work on this problem was done in June 2008 in Haifa resulting in (numerical) solutions for the game and insight in its asymptotic behavior. [1] S. Alpern, The Rendezvous Search Problem, SIAM Journal on Control and Optimization 33 (1995), 673â683. [2] S. Alpern and S. Gal, The Theory of Search Games and Rendezvous, Kluwer Academic Publishers, 2003.Electrical Engineering, Mathematics and Computer Scienc
An adaptive agent for negotiating with people in different cultures
No full textThe rapid dissemination of technology such as the Internet across geographical and ethnic lines is opening up opportunities for computer agents to negotiate with people of diverse cultural and organizational affiliations. To negotiate proficiently with people in different cultures, agents need to be able to adapt to the way behavioral traits of other participants change over time. This article describes a new agent for repeated bilateral negotiation that was designed to model and adapt its behavior to the individual traits exhibited by its negotiation partner. The agent's decision-making model combined a social utility function that represented the behavioral traits of the other participant, as well as a rule-based mechanism that used the utility function to make decisions in the negotiation process. The agent was deployed in a strategic setting in which both participants needed to complete their individual tasks by reaching agreements and exchanging resources, the number of negotiation rounds was not fixed in advance and agreements were not binding. The agent negotiated with human subjects in the United States and Lebanon in situations that varied the dependency relationships between participants at the onset of negotiation. There was no prior data available about the way people would respond to different negotiation strategies in these two countries. Results showed that the agent was able to adopt a different negotiation strategy to each country. Its average performance across both countries was equal to that of people. However, the agent outperformed people in the United States, because it learned to make offers that were likely to be accepted by people, while being more beneficial to the agent than to people. In contrast, the agent was outperformed by people in Lebanon, because it adopted a high reliability measure which allowed people to take advantage of it. These results provide insight for human-computer agent designers in the types of multicultural settings that we considered, showing that adaptation is a viable approach towards the design of computer agents to negotiate with people when there is no prior data of their behavior. © 2011 ACM.Adair Wendi Lyn, 2004, HDB NEGOTIATION CULT, P158; Bohnet I, 2008, AM ECON REV, V98, P294, DOI 10.1257-aer.98.1.294; BUSINESWEEK, 2007, BLOOMBERG BUSINESSWE; BYDE A., 2003, P 4 ACM C EL COMM, P198; Camerer C, 2003, BEHAV GAME THEORY EX; Charness G, 2002, Q J ECON, V117, P817, DOI 10.1162-003355302760193904; DEDREU CKW, 1995, PERS SOC PSYCHOL B, V21, P1178, DOI 10.1177-01461672952111006; Ficici S. G., 2008, P 7 INT JOINT C AUT; GAL Y., 2007, P 6 INT INT C MOD US; GAL Y., 2007, P 22 NAT C ART INT A; Gal Y, 2010, ARTIF INTELL, V174, P1460, DOI 10.1016-j.artint.2010.09.002; Gelfand M. J., 2004, HDB NEGOTIATION CULT; Gelfand MJ, 1999, ORGAN BEHAV HUM DEC, V79, P248, DOI 10.1006-obhd.1999.2845; Gelfand MJ, 2002, J APPL PSYCHOL, V87, P833, DOI 10.1037--0021-9010.87.5.833; Gelfand MJ, 1999, J APPL PSYCHOL, V84, P721, DOI 10.1037--0021-9010.84.5.721; GROSZ B., 2004, P 3 INT JOINT C AUT; Henrich J, 2000, AM ECON REV, V90, P973, DOI 10.1257-aer.90.4.973; Hofstede G., 1980, CULTURES CONSEQUENCE; IMAI L., 2010, ORG BEHAV HUM DECIS; Jonker CM, 2007, AUTON AGENT MULTI-AG, V15, P221, DOI 10.1007-s10458-006-9009-y; KAMAR E., 2008, P INT C AUT AG MULT; Kashima Y, 2005, EUR J SOC PSYCHOL, V35, P147, DOI 10.1002-ejsp.237; KATZ R., 2006, P 5 INT C AUT AG MUL; Kraus S, 2008, ARTIF INTELL, V172, P1, DOI 10.1016-j.artint.2007.05.007; KRAUS S, 1995, COMPUT INTELL, V11, P132, DOI 10.1111-j.1467-8640.1995.tb00026.x; Lin R, 2010, COMMUN ACM, V53, P78, DOI 10.1145-1629175.1629199; Lin R., 2009, P 8 INT C AUT AG MUL, P345; Lount RB, 2008, PERS SOC PSYCHOL B, V34, P1601, DOI 10.1177-0146167208324512; MURPHY R., 2004, IEEE T SYST MAN CY A, V34, P2; Oshrat Y., 2009, P 8 INT C AUT AG MUL, P377; RAJARSHI D., 2001, P 17 INT JOINT C ART; ROTH AE, 1991, AM ECON REV, V81, P1068; SCHURR N., 2006, P 5 INT C AUT AG MUL; TALMAN S., 2005, P 4 INT JOINT C AUT; Weingart L., 2004, HDB NEGOTIATION CULT, P143; Zuckerman I, 2007, 20TH INTERNATIONAL JOINT CONFERENCE ON ARTIFICIAL INTELLIGENCE, P156340
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