24 research outputs found

    GROWTH IMPROVEMENT OF TOMATO WITH THE APPLICATION OF BACTERIAL ISOLATES PRODUCING INDOLE ACETIC ACID (IAA) AND PHOSPHATE SOLUBILIZER

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    Soil bacteria have important roles in biogeochemical cycle for soil fertility and have been manipulated for ecologically-friendly crop production.  The search for beneficial association between microbes and plants for promoting growth and health should be studied for tomato growth improvement. The study aimed to  evaluate 19 microbial isolates which produced indole acetic acid (IAA) affecting growth and development of tomato (Palupi variety), and  molecularly identify the most effective isolates in improving tomato growth based on 16s rDNA sequences. The experiment was conducted in pots using a complete randomized design with three replications. The parameters observed included plant height, plant dry weight, root length, root dry weight, and fruit fresh weight.  The isolates that significantly improved tomato growth were molecularly identified using 16s rRNA sequence. The phenotypic properties such as IAA content and phosphate solubilizing index (PI) of the superior isolates were determined. Results showed that the application of bacterial isolates on tomato significantly increased plant dry weight and fruit yield. From 19 isolates tested, Aj 3.7.1.14 significantly increased plant dry weight, root length, and fruit yield. This isolate produced IAA of about 14.77 ppm and PI of 1.86.  Molecular analysis on Aj 3.7.1.14 demonstrated that the isolate had 89% similarity to Pseudomonas fragi. The identified P. fragi was found to be the most effective isolate for improving tomato growth and fruit yield. Another isolate, Bacillus amyloliquefaciens was found to promote root length, root dry weight, and fruit yield. These isolates are potential to be further investigated for field trial

    Penyebaran TRICHODERMA SPP. dan PENICILLIUM SPP. dan Sifat Antagonismenya Terhadap PHYTOPHTORA PALMIVORA

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    Distribution of Trichoderma spp. and Penicillium spp. and their antagonism to Phytophthora palmivora.The distribution of Trichoderma harzianum, T. viridae and Penicillium spp. and their antagonism to Phytophthora palmivora were studied by isolating the fungi and in vitro test of antagonist. Soil samples were collected from Sukamulya (West Java) and Petaling (Bangka) Exp. Garden, farmer’s garden in the distric of Cahayanegeri (North Lampung) and Sukadana (Central lampung). The distribution of the fungi varied from one place to another. The highest population was found at Sukamulya Exp. Garden and the lowest was at the farmer’s garden in Sukadana. The population difference was assumed to correlate with cultural practices, vegetation and the climate. Trichoderma spp. developed lysis on mycelium and zoospore germination P. palmivora. Penicillium spp. caused mal-formation of mycelium, aborted sporangium and inhibited zoospore germination of the fungus

    WARTA BIOGEN

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    12 hal.; 20c

    Efikasi in Vitro Pelet Erwinia sp BST4 dan Trichoderma harzianum Blt1 Dalam Menekan Infeksi Phytophthora capsici Pada Lada

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    Foot rot disease pf peppercaused by Phyrophthora capsici is a limiting factor in Pepper cultivation since initial symptom of the Disease was difficult to be observed, it was Commonly fail on application curative Treatment. This study was aimed to test the Ability of a mixtuse biocontroll agents Formulated as pellet. Antagonistic activity was Tested on dual culture. Bacterial antagonists Were identified based on physiological and Biochemical characters. Pellet formulation for Trichodema harzianum Blrl was used as basic Formulation Results showed that Erwinia sp. BST4 Viable in pellet formulation without Affected the growth of T. Harzianum Bltl.In in Viva test. At one month after application of Pellet. T. Harzianum pellet or Erwina sp. At the Rate of 10 g/200 g inoculum inhibited the Percentage of colonization of P. Capsici will Decrease when T. Harzianum was mixed with Erwina sp. In the same pellet

    Pencemaran Hasil Panen Tanaman Obat Oleh Mikroorganisma

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    Contamination of medicinal herbs by microorganismPost harvest processing of medicinal herb in handled tradisionally in long periods. The conditions of processing might be trend to contaminated by microorganism. The contaminant is harmful to consumer health and cousing a claim on export. Some microorganism which known to contaminated medicinal herbs material are bacterial group Pseudomonas solanacearum, Xanthomonas sp.; Staphylococcus albus, S. aureus; S. alpha; Bacillus, Proteus, and Coliform and fungae group i.e. Aspergillus flavus; A. niger; rhizopus sp., Sclerotium sp., Rhizoctonia sp.; Penicillium sp.; Fusarium axysporum; F. moniliforme; Fusarium spp. and Gliocladium sp.Once of the some alternative to control the medicinal herbs contamination in investation control including field control and improve storage processing
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