1,721,198 research outputs found
Understand The Role of Calcium in Cardiac Arrhythmias, Studies of Two Mouse Models: FKBP12.6 and Cav1.3-null Mutant Mice.)
No full textsi tratta di una interessante valutazione del ruolo di alcuni canali del Calcio in aritmie cardiache in modelli murin
Analisi dei marcatori apoptotici nell ́ischemia cardiaca mediante tissue microarray
No full textSi tratta di un'analisi effettuata con tissue microarray dell'espressione della superossido dismutasi extracellulare in cuori umani normali ed ischemic
Cd117-Positive Cardiac Primitive Cells: Their Localization And Interaction With Extracellular Matrix In The Human Adult Normal And Pathological Heart
No full textil lavoro descrive la localizzazione di cellule primitive CD-117+ in miocardio umano e la migrazione di queste cellule in corso di patologia ischemic
Expression and intracellular localization of Pyk2 in normal and v-src transformed chicken epiphyseal chondrocytes.
No full textThe expression and localization of prolin-rich tyrosine kinase 2 (Pyk2) were studied in chick embryo epiphyseal chondrocytes. Two immunoreactive bands were detected in chondrocytes, a major band with an apparent Mr of 123 kDa and a minor band with an apparent Mr of 68 kDa. The major band appears to migrate as a doublet with apparent Mr of 116/123 kDa. Increased levels of the three forms of Pyk2 were observed in v-src transformed chondrocytes as compared to control uninfected chondrocytes. Immunofluorescent staining shows that Pyk2 isclearly visible in the cytosol and in the perinuclear region of control and v-src-chondrocytes and displays a pattern very similar to the distributionof the mitochondrial marker Mito Tracker. More, immunofluorescent staining shows that Pyk2 is nuclear in most chondrocytes. By subcellular fractionation, the p116/123 Pyk2 doublet, was found to be accumulated mainly in the cytoplasm while the p68 Pyk2 form, was found to be accumulated exclusively in the nucleus. The differential nuclear/cytoplasmic distribution of the Pyk2 forms remains unchanged after v-Src-induced transformation. The p68 Pyk2 form could no longer be detected by using a N-terminus domain-specific anti-Pyk2 antibody. Consistently, Pyk2 immunoreactivity was restricted to the cytoplasm of control and v-src transformed chondrocytes. Thus it appears that the p68 Pyk2 form that accumulates in the nucleus has a deletion in the N-terminus region
Introducing bioresorbable scaffolds into the show. A potential adjunct to resuscitate Ross procedure
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Different titanium surfaces modulate the bone phenotype of SaOS-2 osteoblast-like cells.
Get PDFCommercially pure titanium implants presenting a relatively smooth, machined surface or a roughned endosseous surface show a large percentage of clinical successes. Surface properties of dental implants seem to be important with respect to bone cells response. Implant topography appears to modulate cell growth and differentiation of osteoblasts affecting the bone healing around the titanium implant. The aim of the present study was to examine the effects of three different titanium surfaces on cell morphology, adhesion and bone phenotypic expression of human osteoblast-like cells, SaOS-2. SaOS-2 cells were cultured on commercially pure titanium disks of 1 cm in diameter with three different surface roughness: smooth (S), sandblasted (SB) and titanium plasma sprayed (TPS). Differences in the cell morphology were found on the three surfaces showing an uniform monolayer of shaped cells on the S surfaces, and clusters of multilayered cells with an irregular shape on the rough surfaces. The adhesion of SaOS-2 cells, as measured after 3h of culture, was not affected by surface roughness. ECM components such as collagen I (CoI), fibronectin (FN), vitronectin (VN) and tenascin (TN) were secreted and organized only on SB and TPS surfaces while on S surfaces they remained in the cytoplasm. Osteopontin and BSP-II were largely detected on SB and TPS surfaces, while only minimal production was observed on S surfaces. These data show that titanium surface roughness affects bone differentiation of osteoblast like-cells, SaOS-2, indicating that surface properties may be able to modulate the osteoblast phenotype. These observations also suggest that the bone healing response around dental implants can be affected by surface topography
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