6 research outputs found

    CORRELATION STUDIES AND PATH COEFFICIENT ANALYSIS FOR SEED YIELD AND YIELD COMPONENTS IN ETHIOPIAN CORIANDER ACCESSIONS

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    Coriander ( Coriandrum sativum L.) is an annual spice herb that belongs to the family Umbelliferae. Even though Ethiopia is a centre of primary diversity for the crop, the current knowledge about its biology, variety development and agronomy is neither complete nor conclusive under Ethiopian conditions. To contribute to filling some of the existing gaps, a field experiment was conducted during the main rainy season of 2007-2008 at Wondo Genet and Kokate, southern Ethiopia. Data for 15 agronomic and quality traits were measured and statistically tested. More of the traits were found having high correlation coefficients at genotypic level than the phenotypic level, demonstrating intrinsic associations among the traits. Seeds plant-1 and thousand seeds weight were associated significantly and positively with seed yield plant-1 at phenotypic and genotypic levels. Essential oil and fatty oil contents were negatively associated with most of the trait studied. Path analysis revealed that days to end 50% flowering, longest basal leaf length, plant height, days to 50% maturity and seeds umbellet-1 exerted positive direct effect on seed yield plant-1, indicating that selection using these traits would be effective in improving seed yield in coriander.Le Coriander ( Coriandrum sativum L.) est une herbe d’épice annuelle qui appartient à la famille Umbelliferae. Même si l’Ethiope est un centre de sa diversité primaire, la connaissance actuelle de sa biologie, son développement variétal et son agronomie ne sont jamais exhaustif ni conclusive en conditions ethiopiennes. Pour contribuer à combler cette brèche, un essai en champ était effectué durant la principale saison de pluie 2007-2008 à Wondo Genet et Kokate, Sud Ethiopie. Les données de 15 traits agronomiques et traits de qualité étaient mesurées et statistiquement testées. Laplupart des traits ont manifesté des coefficients de corrélation élevée au niveau génotypique qu’ au niveau phénotypique, témoignant des associations intrinsèques parmi les traits. Le nombre de grains par plante et le poids de mille grains étaient significativement associés et positivement avec le rendement en grains par plante aux niveaux phénotypiques et génotypiques. Les teneurs en huile essentielle et matière grasse étaient négativement associées à laplupart des traits étudiés. L’analyse du passage a révélé que les jours à 50% de la floraison, la plus longue base de la longueur des fueilles, la hauteur de plants, les jours à 50% de la maturité et le nombre de grains par umbellet ont excercé un effet positif direct sur le rendement en grains par plant, indicant que cette sélection par l’utilisation de ces traits pourrait être efficace en amelioration du rendement en grains coriander

    Performance of African marigold (Tagetes errecta) cultivars for vegetative, flower and chemical traits at different locations of Ethiopia

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    Despite the African marigold (Tagetes erecta L.) is one of the most commercially exploited flower and medicinal crops in the world and have received great attention in scientific research in the recent past, the plant has never been tested under Ethiopian condition for its agronomic and chemical traits. Thus, the present study was conducted with the main objective to evaluate the growth, yield and quality attributes of three introduced African marigold cultivars. The experiments were conducted at six locations in Ethiopia using a randomized complete block design with three replications. Data on vegetative growth, flower characteristics, inflorescence yield, and xanthophyll content were collected and statistically tested. Significant variation (P < 0.01) was observed among cultivars across the tested locations for all the studied parameters. Overall higher values of plant height (65.48 cm), branch number plant-1 (89.09), inflorescence number plant-1 (76.89), inflorescence weight (25.48 g), inflorescence yield plant-1 (560.77 g) and inflorescence yield ha-1 (18 t) were obtained in AVT001 cultivar and flower xanthophyll content (27.6 mg g-1) in AVT540 cultivar; while lowest values were rerecorded for all characters in Hewoyde cultivar. Inflorescence yield ha-1 was found positively and significantly correlated with all parameters except xanthophyll content. Xanthophyll content was found positively and significantly correlated with flower weight. As the cultivars AVT001 and AVT540 are adapted very well and gave comparable and/or higher yields with the other marigold growing countries, the two cultivars can be recommended for commercial cultivation of their inflorescence and extracted pigments in Ethiopia

    CORRELATION STUDIES AND PATH COEFFICIENT ANALYSIS FOR SEED YIELD AND YIELD COMPONENTS IN ETHIOPIAN CORIANDER ACCESSIONS

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    Coriander ( Coriandrum sativum L.) is an annual spice herb that belongs to the family Umbelliferae. Even though Ethiopia is a centre of primary diversity for the crop, the current knowledge about its biology, variety development and agronomy is neither complete nor conclusive under Ethiopian conditions. To contribute to filling some of the existing gaps, a field experiment was conducted during the main rainy season of 2007-2008 at Wondo Genet and Kokate, southern Ethiopia. Data for 15 agronomic and quality traits were measured and statistically tested. More of the traits were found having high correlation coefficients at genotypic level than the phenotypic level, demonstrating intrinsic associations among the traits. Seeds plant-1 and thousand seeds weight were associated significantly and positively with seed yield plant-1 at phenotypic and genotypic levels. Essential oil and fatty oil contents were negatively associated with most of the trait studied. Path analysis revealed that days to end 50% flowering, longest basal leaf length, plant height, days to 50% maturity and seeds umbellet-1 exerted positive direct effect on seed yield plant-1, indicating that selection using these traits would be effective in improving seed yield in coriander.Le Coriander ( Coriandrum sativum L.) est une herbe d’épice annuelle qui appartient à la famille Umbelliferae. Même si l’Ethiope est un centre de sa diversité primaire, la connaissance actuelle de sa biologie, son développement variétal et son agronomie ne sont jamais exhaustif ni conclusive en conditions ethiopiennes. Pour contribuer à combler cette brèche, un essai en champ était effectué durant la principale saison de pluie 2007-2008 à Wondo Genet et Kokate, Sud Ethiopie. Les données de 15 traits agronomiques et traits de qualité étaient mesurées et statistiquement testées. Laplupart des traits ont manifesté des coefficients de corrélation élevée au niveau génotypique qu’ au niveau phénotypique, témoignant des associations intrinsèques parmi les traits. Le nombre de grains par plante et le poids de mille grains étaient significativement associés et positivement avec le rendement en grains par plante aux niveaux phénotypiques et génotypiques. Les teneurs en huile essentielle et matière grasse étaient négativement associées à laplupart des traits étudiés. L’analyse du passage a révélé que les jours à 50% de la floraison, la plus longue base de la longueur des fueilles, la hauteur de plants, les jours à 50% de la maturité et le nombre de grains par umbellet ont excercé un effet positif direct sur le rendement en grains par plant, indicant que cette sélection par l’utilisation de ces traits pourrait être efficace en amelioration du rendement en grains coriander

    Rapid delivery of Cas9 gene into the tomato cv. 'Heinz 1706' through an optimized Agrobacterium-mediated transformation procedure

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    © 2021 Centro Regional de Invest. Cientif. y Tecn.. All rights reserved.Solanum lycopersicum 'Heinz 1706' is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in the transfection approach. Thus, this study was designed to generate Cas9-expressing 'Heinz 1706' lines by establishing an Agrobacterium tumefaciens-mediated transformation (ATMT) procedure. Here, we report a rapid and reproducible transformation procedure for 'Heinz 1706' by finetuning various factors: A. tumefaciens strain, pre-culture and co-culture durations, a proper combination of phytohormones at each step, supplementation of acetosyringone, and shooting/rooting method. Particularly, through eluding subculture and simultaneously inducing shoot elongation and rooting from leaf cluster, we achieved a short duration of three months for recovering the transgenic plants expressing Cas9. The presence of the Cas9 gene and its stable expression were confirmed by PCR and qRT-PCR analyses, and the Cas9 gene integrated into the T0 plant genome was stably transmitted to T1 progeny. Therefore, we anticipate that our procedure appears to ease the conventional ATMT in 'Heinz 1706', and the created Cas9-expressing 'Heinz 1706' lines are ultimately useful in gene editing via unilateral transfection of sgRNA into the protoplasts.11Nsciescopu

    Nhân giống cây Oải Hương (Lavandula angustifolia Mill.) bằng phương pháp giâm hom

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    Oải hương Anh (Lavandula angustifolia) là loài cho sản lượng và hàm lượng tinh dầu cao, có thể sinh trưởng và phát triển tốt ở vùng có khí hậu ôn đới.. Vì đây là loài mới được du nhập vào Việt Nam trong những năm gần đây, nên các nghiên cứu về nhân giống Oải hương Anh để đáp ứng nhu cầu nguồn giống sản xuất còn hạn chế. Nhóm nghiên cứu đã khảo sát các yếu tố ảnh hướng đến quá trình giâm hom Oải hương Anh. Kết quả nghiên cứu cho thấy ngọn, ngọn bánh tẻ hoặc phần thân non dưới ngọn của cây mẹ khỏe, không bị sâu bệnh đều phù hợp làm hom giâm. Các hom này được xử lý với 100 mg/l NAA trong 1 phút sau đó giâm vào vỉ xốp có chứa xơ dừa vào mùa mưa (tháng 3-10) cho tỷ lệ sống cao (100%); chất lượng rễ tốt với chiều dài rễ 4,8 cm và số lượng rễ 20,4 rễ/hom sau 3 tuần. Kết quả nghiên cứu có thể được áp dụng trên quy mô lớn hơn để tạo ra số lượng cây giống đồng đều chất lượng và khỏe mạnh, đáp ứng yêu cầu về nguồn giống để phát triển loài này.4056/202129-3

    Nhân giống cây Oải Hương (Lavandula angustifolia Mill.) bằng phương pháp giâm hom

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    Oải hương Anh (Lavandula angustifolia) là loài cho sản lượng và hàm lượng tinh dầu cao, có thể sinh trưởng và phát triển tốt ở vùng có khí hậu ôn đới.. Vì đây là loài mới được du nhập vào Việt Nam trong những năm gần đây, nên các nghiên cứu về nhân giống Oải hương Anh để đáp ứng nhu cầu nguồn giống sản xuất còn hạn chế. Nhóm nghiên cứu đã khảo sát các yếu tố ảnh hướng đến quá trình giâm hom Oải hương Anh. Kết quả nghiên cứu cho thấy ngọn, ngọn bánh tẻ hoặc phần thân non dưới ngọn của cây mẹ khỏe, không bị sâu bệnh đều phù hợp làm hom giâm. Các hom này được xử lý với 100 mg/l NAA trong 1 phút sau đó giâm vào vỉ xốp có chứa xơ dừa vào mùa mưa (tháng 3-10) cho tỷ lệ sống cao (100%); chất lượng rễ tốt với chiều dài rễ 4,8 cm và số lượng rễ 20,4 rễ/hom sau 3 tuần. Kết quả nghiên cứu có thể được áp dụng trên quy mô lớn hơn để tạo ra số lượng cây giống đồng đều chất lượng và khỏe mạnh, đáp ứng yêu cầu về nguồn giống để phát triển loài này.4056/202129-3
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