1,721,037 research outputs found

    Authenticating ancient human mitochondrial DNA

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    The use of ancient DNA techniques in human studies has been hampered by problems of contamination with modern human DNA. The main problem has been that the object of study belongs to the same species as the observer, and the complete elimination of the contamination risk is seemingly unlikely. Contamination has even been detected in the most specialized laboratories in this field. In these kinds of studies it is therefore very important to detect contamination and to distinguish contaminants from authentic results. Here, we report the use of a strategy to authenticate the identity of ancient mitochondrial DNA (mtDNA), based on the previously established relationship between D-loop sequence substitutions and haplogroup-specific restriction site changes. Forty-four individuals from a 16th-century necropolis were analyzed, from which 28 control region sequences were obtained. These sequences were preclassified into haplogroups, according to the observed motifs. Subsequently, the DNA extracts from which the sequences were obtained, along with independent extracts of subsets of the same individuals, were subjected to restriction fragment length polymorphism (RFLP,) analysis to compare and corroborate the results. Using this approach, 24 sequences were authenticated, while two were discarded because of result mismatches. The final distribution of the haplogroups in the sample, and the differences in the sequences, are two additional criteria of authentication

    A congenital sternum Anomaly from a late Roman burial from Mallorca, Spain.

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    A curious case of a congenital non fusion of the caudal portion of the sternum was found in the skeletal remains of a juvenile male recovered from a Late Roman burial from the Necropolis of Pollentia, Mallorca, Spain. No other significant changes in the skeleton or pathological disorders were observed and potentially health problems, due to this anomaly, can not be determined. This kind of congenital defect is unusual and there are a few cases reported in paleopathological literature. Despite the fact that the sternum is one of the bones of the body with a wider variety of congenital variants, generally the described cases focus on the most frequent anomalies, such as pectus excavatum or sternal aperture, whereas the rarest variations, such as sternal bifid/cleft or failure of fusion of the caudal portion are not reported

    Etude préliminaire des systèmes sanguins Rh et Kell dans un échantillon de population de la Cerdagne catalane

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    PRELIMINARY STUDY OF Rh AND KELL BLOOD GROUPS IN A CATALONIAN - CERDANYA COUNTRY Summary. — This work reports the results obtained in the study of Rh and Kell systems in the Catalonian — Cerdanya Country. Our data were compared with those of Spanish and East French Pyrenees populations.Résumé. — Dans ce travail, nous avons étudié les systèmes sanguins Rh et Kell dans un échantillon représentatif de la Cerdagne Catalane. Les résultats sont comparés avec ceux qui ont été trouvés dans d'autres endroits de l'Espagne et des Pyrénées françaises.Aluja , Malgosa A., Font A. Etude préliminaire des systèmes sanguins Rh et Kell dans un échantillon de population de la Cerdagne catalane. In: Bulletins et Mémoires de la Société d'anthropologie de Paris, XIV° Série. Tome 2 fascicule 4, 1985. pp. 237-246

    XRF investigation on skeletal remains from King Peter III of Aragon (1239-1285 A.D.) and Queen Blanche of Anjou (1280-1310 A.D.)

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    We conducted an X-Ray Fluorescence investigation on bone fragments belonging to King Peter III of Aragon and Queen Blanche of Anjou. The spectroscopic analysis was carried out in selected points of the bone fragments. Several transitional elements normally unexpected in the bone composition have been found at varying level of concentration. The presence of these elements was interpreted in relation to chemical treatments for mummification of bodies as well as to dietary habits, including tools used for cooking and for the consumption of food

    The study of dental occlusion in ancient skeletal remains from Mallorca (Spain): a new approach based on dental clinical practice

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    Occlusal characteristics, fundamental to assess the presence of malocclusion, have been often unexplored in bioarchaeological analyses. This is largely due to the fragmented condition of the skeletal remains. By applying a method that considers dental and maxillary features useful to evaluate occlusion in ancient fragmentary material, the purpose of this work is to define the occlusal features and explore the causes of malocclusion in a mediaeval population from Mallorca. The findings of this study suggest that normocclusion was present in ca. 60% of the individuals (N = 31), and that some characteristics, such as molar relationship, were slightly different from those of modern populations. The analysis of the occlusal features revealed for example that open-bite was absent in 85% of the sample, posterior open-bite was completely absent and overbite and overjet were normal in around 90% of the individuals. Statistically significant correlations between canine and molar relationships and between molar relationship and dental wear of the superior and inferior canines and incisors were observed. In addition, wear could affect the curve of Spee. All these findings strengthen the hypothesis that in ancient times malocclusion was not as generalized as in modern times. Although the factors that lead to malocclusion throughout centuries could have several causes, we suggest that in this population dental wear, which is strongly associated with the diet, was the fundamental causing factor

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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