171 research outputs found
Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function
No full textThe CCAAT box is one of the most common elements in eukaryotic promoters and is activated by NF-Y, a conserved trimeric transcription factor with histone-like subunits. Usually one CCAAT element is present in promoters at positions between -60 and -100, but an emerging class of promoters harbor multiple NF-Y sites. In the triple CCAAT-containing cyclin B2 cell-cycle promoter, all CCAAT boxes, independently from their NF-Y affinities, are important for function. We investigated the relationships between NF-Y and p300. Chromatin immunoprecipitation analysis found that NF-Y and p300 are bound to the cyclin B2 promoter in vivo and that their binding is regulated during the cell cycle, positively correlating with promoter function. Cotransfection experiments determined that the coactivator acts on all CCAAT boxes and requires a precise spacing between the three elements. We established the order of in vitro binding of the three NF-Y complexes and find decreasing affinities from the most distal Y1 to the proximal Y3 site. Binding of two or three NF-Y trimers with or without p300 is not cooperative, but association with the Y1 and Y2 sites is extremely stable. p300 favors the binding of NF-Y to the weak Y3 proximal site, provided that a correct distance between the three CCAAT is respected. Our data indicate that the precise spacing of multiple CCAAT boxes is crucial for coactivator function. Transient association to a weak site might be a point of regulation during the cell cycle and a general theme of multiple CCAAT box promoters
Cyclin B1 transcription is enhanced by the p300 coactivator and regulated during the cell cycle by a CHR-dependent repression mechanism
No full textAbstractCyclin B is a central regulator of transition from the G2 phase of the cell cycle to mitosis. In mammalian cells two B-type cyclins have been characterised, cyclin B1 and B2. Both are expressed with a maximum in G2 and their synthesis is mainly regulated on the transcriptional level. We show that a single cell cycle genes homology region, lacking a functional cell cycle-dependent element in tandem with it, contributes most of the cell cycle-dependent transcription from the cyclin B1 promoter. The coactivator p300 binds to the cyclin B1 promoter and synergises with the transcription factor NF-Y in activating transcription of cyclin B1
The cyclin B2 promoter depends on NF-Y, a trimer whose CCAAT-binding activity is cell-cycle regulated
No full textCyclin B2 is a regulator of p34cdc2 kinase, involved in G2/M progression of the cell cycle, whose gene is strictly regulated at the transcriptional level in cycling cells. The mouse promoter was cloned and three conserved CCAAT boxes were found. In this study, we analysed the mechanisms leading to activation of the cyclin B2 CCAAT boxes: a combination of (i) genomic footprinting, (ii) transfections with single, double and triple mutants, (iii) EMSAs with nuclear extracts, antibodies and NF-Y recombinant proteins and (iv) transfections with an NF-YA dominant negative mutant established the positive role of the three CCAAT sequences and proved that NF-Y plays a crucial role in their activation. NF-Y, an ubiquitous trimer containing histone fold subunits, activates several other promoters regulated during the cell cycle. To analyse the levels of NF-Y subunits in the different phases of the cycle we separated MEL cells by elutriation, obtaining fractions > 80% pure. The mRNA and protein levels of the histone-fold containing NF-YB and NF-YC were invariant, whereas the NF-YA protein, but not its mRNA, was maximal in mid-S and decreased in G2/M. EMSA confirmed that the CCAAT-binding activity followed the amount of NF-YA, indicating that this subunit is limiting within the NF-Y complex, and suggesting that post-transcriptional mechanisms regulate NF-YA levels. Our results support a model whereby fine tuning of this activator is important for phase-specific transcription of CCAAT-containing promoters
Wasner, Charles (Birth, 1905-10-28)
No full textAddress: Wheeler St.4838/Pg.112/1905/M W/Cinti,Ohio/Cinti,Ohio/B. Schuster, Mid.Original record filed in drawer labeled 'WARMAN- WEBENSTET'
The Bridge: A Yearbook of Judaeo-Christian Studies, Vol. 4
No full textIntroduction/John M. Oesterreicher
Surveys: The God of Israel, God of Love / Kathryn Sullivan, R.S.C.J. Israel in the Parables / Mirjam Prager, O.S.B. The Song of Songs: The Rhetoric of Love / Barry Ulanov Love of God in the Talmud / Joseph P. Brennan The Covenant of Husband and Wife / Edward A. Synan The Tragedy of the Spanish Inquisition / Paul van K. Thomson Franz Werfel\u27s Great Dilemma / Frederick C. Ellert
Perspectives: St. Paul: Liberty and Law / Stanislas Lyonnet, S.J. Baḥya Ibn Paḳuda, Tutor of Hearts / M. Raffaella de Sion and Edward A. Synan The Popes\u27 Veneration of the Torah / Franz Wasner St. Ignatius Loyola and the Jews / James J. Brodrick, S.J. The Face of Pasternak / Cornelia and Irving Süssman A Note on the Art of Ernst Fuchs / Barry Ulanov
Surveys: Three Voices at Basel / Herbert Haag The Swastika Reappears / John M. Oesterreicher Israeli Reactions / Josephine D. Casgrain
Book Review
Effects of spiritual care training for palliative care professionals
No full textLittle is known about the effects of spiritual care training for professionals in palliative medicine. We therefore investigated prospectively the effects of such training over a six-month period. All 63 participants of the three and a half-day training were asked to fill out three questionnaires: before and after the training, as well as six months later. The questionnaires included demographic data, numeric rating scales about general attitudes towards the work in palliative care, the Self-Transcendence Scale (STS), the spiritual subscale of the Functional Assessment of Chronic Illness Therapy (FACIT-Sp) and the Idler Index of Religiosity (IIR). Forty-eight participants (76) completed all three questionnaires (91 women, median age 49 years; 51 nurses, 16 hospice volunteers, 14 physicians).Significant and sustained improvements were found in self-perceived compassion for the dying (after the training: P =0.002; 6 months later: P=0.025), compassion for oneself (P < 0.001; P =0.013), attitude towards one's family (P =0.001; P =0.031), satisfaction with work (P < 0.001; P =0.039), reduction in work-related stress (P < 0.001; P =0.033), and attitude towards colleagues (P =0.039; P =0.040), as well as in the FACIT-Sp (P < 0.001; P =0.040). Our results suggest that the spiritual care training had a positive influence on the spiritual well-being and the attitudes of the participating palliative care professionals which was preserved over a six-month period
Bemerkungen zu Heinrich v. Kleists Hermannsschlacht, ein Beitrag zum Kapital der Schullekture.
No full textProgramm - Regensburg.Mode of access: Internet
- …
