85 research outputs found
The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis
No full textAlshaymaa M Alhabibi,1 Maisa A Wahab,2 Ahmed Khairy Sakr,2 Samar M Abd El-Hamid,1 Mohamed Yahia Zakaria,3 Azza Ali Althoqapy,4 Hanaa Mohammed Eid El Sayed,5 Sammar Ahmed Kasim,5 Hanan F Ibrahim,4 Ola I Saleh,6 Howida A Ahmed,6 Alaa H Sayed,7 Mahmoud Lotfy8 1Department of Clinical Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 2Department of Vascular Surgery, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 3Department of Vascular and EndoVascular Surgery, Faculty of Medicine for Boys, Al-Azhar University, Cairo, Egypt; 4Medical Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 5Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 6Department of Radio-Diagnosis, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 7Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt; 8Molecular Biology Department, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya, EgyptCorrespondence: Alshaymaa M Alhabibi, Tel +201002894075, Email [email protected]: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used as markers of venous thrombosis and to identify whether there was a link between NK cell proportion and citrullinated histone (H3) levels.Patients and Methods: This study included 100 participants divided into Group I (n=50, patients with deep venous thrombosis (DVT)) and Group II (n=50, age- and sex-matched healthy controls). Group I was further categorized into Group Ia (n=25, patients with acute DVT) and Group Ib (n=25, patients with chronic DVT). The proportions of NK cells and their subsets were evaluated by flow cytometry using CD3/CD16/CD56. The levels of citrullinated histones (H3) were estimated using enzyme-linked immunosorbent assay (ELISA).Results: Compared to the control group, DVT patients had a significantly lower proportion of (CD56 dim/CD16+) NK cells, a significantly higher proportion of (CD56-/CD16+) NK cells and a high level of citrullinated histone (H3).Conclusion: NK cell subsets and citrullinated histone (H3) could be used as markers for DVT and as targets for therapeutic drugs to inhibit the formation or progression of thrombosis.Keywords: natural killer cell, natural killer cell subsets, citrullinated histone, deep venous thrombosi
Synthesis and spectroscopic properties of some pyrimido[4,5-b]quinoliiie derivatives
No full textAuthor: 1, Women Students Medical Studies & Sciences Sections, Chemistry Department,
College of Science, King Saud University
P.O. Box 22452, Riyadh 11495.
Author: 3, From Chemistry Department, College of Science, King Saud University
P.O. Box 2455, Riyadh 11451.Several substituted pyrimido[4,5-b]quinoline were synthesized from the intermediates: 2-chloro-3-quinolinecarbonitrile (1d), 2-amino-3-quinolinecarbonitrile (1h) and 2-oxo-3-quinolinecarboxylic acid (1g), via cyclization with urea, thiourea, guanidine, formamide and acetic anhydride. Structural elucidation of the prepared quinolines and pyrimido[4,5-b]quinolines was mainly based on the spectroscopic methods, and in particular, MS and NMR spectr
Who Or What Is Werethekau ‘Great of Magic’? A Problematic Inscription (UC 16639)
No full textIn this paper, I investigate the identity of Werethekau through a previously unpublished limestone block at the Petrie Museum (UC 16639). It is not recorded when or where this block was found; the context, a central and identifying feature for the archaeological discipline, is lost (Johnson 1999: 107). The Petrie Museum records do not include the method or date of acquisition. I will focus here on the following questions: What does this object represent? Who is Werethekau? Is it an epithet for a goddess, or a name for a material object, or for a goddess frequently associated with the coronation of the king? The discussion also introduces the approach on which I rely for the identification of the object and its chronology. A further aim in this publication is to bring the object to the attention of a wider readership of scholars of Egyptian archaeology in an attempt to find the missing parts
Assessment of circulating fibroblast growth factor 23 in type 2 diabetic patients with nephropathy
No full textEffect of Some Dozer Design Parameters on Dozer Productivity
No full textIn this paper, a study for the effect of design parameters of dozers on their productivity is done by using the productivity-related data given in manuals of three different tracked dozer brands. The major design parameters that have been chosen in the study are the dozer weight, the dozer blade type and blade capacity and they approximately have the same power to weight ratio. Empirical equations for calculating the productivity for each brand have also been developed by using data-fit program. Finally, it has been concluded that the blade capacity stands as the most significant parameter as the dozer productivity increases by sixty percent in average whenever the blade capacity increases by twenty five percent.
Stability Study and Clinical Evaluation of Lipid Injectable Emulsion in Parenteral Nutrition Admixtures Used for Preterm Neonates
No full text5-Aryl-1-Arylideneamino-1H-Imidazole-2(3H)-Thiones: Synthesis and In Vitro Anticancer Evaluation
No full textA novel series of N-1 arylidene amino imidazole-2-thiones were synthesized, identified using IR, 1H-NMR, and 13C-NMR spectral data. Cytotoxic effect of the prepared compounds was carried out utilizing three cancer cell lines; MCF-7 breast cancer, HepG2 liver cancer, and HCT-116 colon cancer cell lines. Imidazole derivative 5 was the most potent of all against three cell lines. DNA flow cytometric analysis showed that, imidazoles 4d and 5 exhibit pre-G1 apoptosis and cell cycle arrest at G2/M phase. The results of the VEGFR-2 and B-Raf kinase inhibition assay revealed that compounds 4d and 5 displayed good inhibitory activity compared with reference drug erlotinib
Biochemical and insecticidal efficacy of clove and basil essential oils and two photosensitizers and their combinations on Aphis gossypii glover (Hemiptera: Aphididae)
No full textThe present study investigates the insecticidal and biochemical effects of two essential oils (EOs) and two photosensitizers against cotton aphids in a laboratory setting. The EOs evaluated were clove (Syzygium aromaticum L.) and basil (Ocimum basilicum), while the photosensitizers were rose bengal and rhodamine B. The individual median lethal concentrations (LC50) revealed that clove was ~4.44 times more potent than basil, and rhodamine B was ~1.34 times more potent than rose bengal. The mortality rates increased using higher concentrations of the photosensitizers and prolonging exposure time to sunlight. The most effective combination against adult aphids was found to be a mixture of sub-lethal concentrations of clove and rhodamine B, resulting in a mortality rate of 92.31%. Conversely, the combination of basil and rose bengal exhibited the lowest efficacy with a mortality rate of 33.33%. Biochemical analyses indicate that Rhodamine B, basil, and the basil-rhodamine B mixture (mixture C) significantly reduced trehalase activity. However, the protease activity significantly increased in aphids treated with rose bengal, clove, and the clove-rose bengal mixtures (mixtures A and B). The lipase activity is notably decreased upon treatment with rhodamine B and clove. Glutathione S-transferase (GST) activity decreased in aphids treated with rose bengal and the basil-rhodamine B mixtures (mixtures C and D), suggesting that GST did not play a role in detoxifying these compounds, thereby explaining the susceptibility of A. gossypii. Overall, the combination of essential oils and photosensitizers has demonstrated a synergistic effect in controlling Aphis gossypii, offering great potential as an effective strategy for aphid management
Efficacy of Selected Chest Physical Therapy on Neonates with Respiratory Distress Syndrome
No full textAbstract: Chest Physiotherapy has been used to clear secretions, prevent accumulation of debris and improve mobilization or airways secretions and help lung ventilation in newborn for respiratory problems. Physical therapy modalities include positioning, postural drainage, percussion, vibration, and suction. The study was established to investigate efficacy of applying selected chest physiotherapy modalities on the neonates with Respiratory Distress Syndrome (RDS). The objective of chest physiotherapy was to increase the clearance of the lung secretions and to maintain lung expansion with the potential benefit of improving oxygenation, prevention of endotracheal tube obstruction and subsequent hypoxia, prevention of extubation failure and minimizing the need to ventilation or the duration of its use and avoiding complications resulting from its use or prolonged exposure to ventilation
Diagnostic Evaluation of Urinary Angiogenin (ANG)and Clusterin (CLU) as Biomarker for Bladder Cancer
No full textBladder carcinoma is an important worldwide health problem. Both cystoscopy and urine cytology used in detecting bladder cancer suffer from drawbacks where cystos- copy is an invasive method and urine cytology shows low sensitivity in low grade tumors. This study validates easier and less time-consuming techniques to evaluate the value of com- bined use of angiogenin and clusterin in comparison and combination with voided urine cytology in the detection of bladder cancer patients. This study includes malignant (blad- der cancer patients, n=50), benign (n=20) and healthy (n=20) groups. The studied groups were subjected to cystoscopic examination, detection of bilharzial antibodies, urine cytolo- gy, and estimation of urinary angiogenin and clusterin by ELISA. The overall sensitivity and specificity were 66 and 75 % for angiogenin, 70 and 82.5 % for clusterin and 46 and 80 % for voided urine cytology. Combined sensitivity of voided urine cytology with the two studied biomarkers was 88 % which is higher than the combined sensitivity of both markers alone (82 %) and that of the cytology with each marker (76 and 80 %) for angiogenin and clusterin respective- ly. In conclusion, combined use of the cytology with the studied biomarkers can improve the sensitivity for detecting bladder cancer, and may be very useful in monitoring the effectiveness of antiangiogenic and apoptotic therapies in bladder cancer
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