104 research outputs found
Personal Papers (MS 80-0002)
No full textLetter from Sol Satinsky to Arthur Alpert discussing Mr. Alpert's nomination to the National Council of the Joint Distribution Committee
Internal Genomic DNA Standard for Quantitative Metagenome Analysis v3
No full textSatinsky, Brandon M., et al. 'Use of internal standards for quantitative metatranscriptome and metagenome analysis.' Methods in enzymology 531 (2012): 237-250. </p
Internal Genomic DNA Standard for Quantitative Metagenome Analysis v1
No full textSatinsky, Brandon M., et al. 'Use of internal standards for quantitative metatranscriptome and metagenome analysis.' Methods in enzymology 531 (2012): 237-250. </p
Preparation of Custom Synthesized RNA Transcript Standard v3
No full textThis protocol is from: Satinsky, Brandon M., et al. "Use of internal standards for quantitative metatranscriptome and metagenome analysis." Methods in enzymology 531 (2012): 237-250. Please see the full chapter for additional details. </p
Preparation of Custom Synthesized RNA Transcript Standard v1
No full textThis protocol is from: Satinsky, Brandon M., et al. "Use of internal standards for quantitative metatranscriptome and metagenome analysis." Methods in enzymology 531 (2012): 237-250. Please see the full chapter for additional details. </p
Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume
No full textThe Amazon River has the largest discharge of all rivers on Earth, and its complex plume system fuels a wide array of biogeochemical processes, across a large area of the western tropical North Atlantic. The plume thus stimulates microbial processes affecting carbon sequestration and nutrient cycles at a global scale. Chromosomal gene expression patterns of the 2.0 to 156 μm size-fraction eukaryotic microbial community were investigated in the Amazon River Plume, generating a robust dataset (more than 100 million mRNA sequences) that depicts the metabolic capabilities and interactions among the eukaryotic microbes. Combining classical oceanographic field measurements with metatranscriptomics yielded characterization of the hydrographic conditions simultaneous with a quantification of transcriptional activity and identity of the community. We highlight the patterns of eukaryotic gene expression for 31 biogeochemically significant gene targets hypothesized to be valuable within forecasting models. An advantage to this targeted approach is that the database of reference sequences used to identify the target genes was selectively constructed and highly curated optimizing taxonomic coverage, throughput, and the accuracy of annotations. A coastal diatom bloom highly expressed nitrate transporters and carbonic anhydrase presumably to support high growth rates and enhance uptake of low levels of dissolved nitrate and CO2. Diatom-diazotroph association (DDA: diatoms with nitrogen fixing symbionts) blooms were common when surface salinity was mesohaline and dissolved nitrate concentrations were below detection, and hence did not show evidence of nitrate utilization, suggesting they relied on ammonium transporters to aquire recently fixed nitrogen. These DDA blooms in the outer plume had rapid turnover of the photosystem D1 protein presumably caused by photodegradation under increased light penetration in clearer waters, and increased expression of silicon transporters as silicon became limiting. Expression of these genes, including carbonic anhydrase and transporters for nitrate and phosphate, were found to reflect the physiological status and biogeochemistry of river plume environments. These relatively stable patterns of eukaryotic transcript abundance occurred over modest spatiotemporal scales, with similarity observed in sample duplicates collected up to 2.45 km in space and 120 minutes in time. These results confirm the use of metatranscriptomics as a valuable tool to understand and predict microbial community function.Gordon and Betty Moore Foundation (River Ocean Continuum of the Amazon Project. Grants GBMF 2293 and 2928)National Science Foundation (U.S.) (Grant NSF-OCE 0934095
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Laboratory evaluation of laparoscopic vascular clamps using a load-cell device--are all clamps the same?
No full textThe use of effective vascular clamps is key to successful laparoscopic partial nephrectomy. Based on our clinical experience the occlusive capabilities of vascular clamps appeared to be quite variable. We compared the occlusive force of currently available laparoscopic vascular clamps.
The jaw force of 3 laparoscopic vascular clamps (Aesculap(R), Klein Surgical Systems, San Antonio, Texas and Karl Storztrade mark) were measured by clamping a 2.2 mm compression load cell (Interface Advanced Force Measurement, Scottsdale, Arizona) in pound-force. The variables tested were handheld Satinsky, DeBakey and Storz clamps vs bulldog clamps, proximal, middle and distal application position, new vs used bulldog clamps and new vs used Satinsky handheld clamps. In addition, handheld clamps were tested according to the force generated by the notches in the locking mechanism. Force retention was also determined for all instruments after clamping a 20Fr latex rubber catheter for an hour. Finally, leak pressure studies were performed using a harvested porcine artery to determine the relationship between jaw force and leak pressure in mm Hg of bulldog and Satinsky handheld clamps using a pressure gauge (Cole-Parmer(R)).
Handheld vascular clamps provided greater force than bulldog clamps. The proximal position closest to the hinge provided the greatest force across all instruments. Compared to new clamps the 2-year-old Klein Surgical Systems bulldog clamps showed a greater than 40% decrease in jaw force at all positions, whereas the 3-year-old Aesculap bulldog clamps decreased in jaw force by less than 9% at all positions. The 2-year-old Satinsky handheld clamps showed a decrease of 20%, 9% and 0% at the distal, middle and proximal jaw positions, respectively. Also, there was a positive correlation between force and the number of notches applied in handheld clamps. In addition, all instruments maintained jaw force after 1 hour of continuous clamping. Finally, leak pressure studies performed with used clamps showed that Klein Surgical Systems bulldog, Aesculap bulldog and Satinsky handheld clamps leaked at a pressure of 153 to 223, 465 to 795 and 1,500 to 2,600 mm Hg, respectively.
Vascular clamps have varying occlusive forces according to clamp type, manufacturer, jaw and teeth characteristics, jaw clamping position and duration of use. However, across all clamps the jaw force was greatest at the proximal position. This is most important when applying laparoscopic bulldog clamps. In contrast, all handheld vascular clamps generated higher force than intracorporeal bulldog clamps. At 1 notch the handheld vascular clamps provided supraphysiological occlusion force regardless of position or manufacturer
Multiculturalism and compassion: Responding to mental health needs among refugees and asylum seekers: Comment on “a crisis of humanitarianism: Refugees at the gates of Europe”
No full textAs Fotaki (2019) argues, the current political climate in Europe is threatening principles of humanitarianism, particularly among refugees and asylum seekers. This commentary builds on that argument, with a spotlight on mental health and culturally relevant service design. By addressing some of the barriers faced by refugees and asylum seekers in accessing mental healthcare, we can address inequalities and develop compassionate societies. © 2019 The Author(s)
Extension - surgical technique
No full textIntroduction: Surgical technique for left renal cell carcinoma with infradiaphragmatic
caval extension is presented.
Technique: A transperitoneal bilateral subcostal approach was used. After left colon reflection, the renal artery is ligated and divided, the left renal vein is dissected on. The
right colon reflection is perform ed, the right renal vein and vena cava is dissected. Satinsky
clamp is placed on infrarenal vena cava, a bulldog clamp is placed on the right renal vein
and a Satinsky clamp is placed on vena cava above the thrombus. Circular incision on
vena cava at the level of left renal vein is performed and en block perifascial nephrectomy
including the thrombus is done. The vena cava incision is repaired with a continuos 4-0 vascular suture. Extensive lymph node dissection is performed.
Conclusions: Out of more than 1305 RCC operated in our Department between 1975
- 2000, 142 cases have had caval extension. Using appropriate surgical technique, the patient's survival is almost similar to those without caval extension
MYTOLOGICKÉ ASPEKTY KLAMSTVA A PRAVDY V SLOVENSKEJ ĽUDOVEJ A AUTORSKEJ ROZPRÁVKE
No full textIn the study the author focuses on the legacy of folktales as represented in a wide scope of traditional and „artistic folktales“ originally aimed at children listeners/readers. In the texts by Pavol Dobsinsky and Samuel Czambel, the author attempted to identify the parallel ideas and also mythological-fairytale parallels related to the themes of selected fairytales and rich in the motifs of truth, justice and also lies and injustice. Characterwise, attention has also been paid to linguistic and stylistic means. Further attention has been paid to pre-set functions of characters in fantasy, heroic and family-social fairytales; the author compared them with more realistic fairytales which often reflect social-economic phenomena as well as attitudes to lies and truth. When characterising the phenomenon of Truth, the author gravely relied on the basic ethical principles of fairytales and these were applied on the well-known fairytale Či jesto pravda na svete (Is there any Truth in the world?) which later served as a prototext for an artistic poetic prose of Milan Rufus. The poet affirmatively processes the prototext, creatively using ethical and humane ideas. Other analysed texts include a modern parodic and nonsense artistic fairytale entitled Rozprávky uja Klobásu (Mr. Saussage‘s Fairytales) by Julius Satinsky, where Lie is a predominant narrator´s standpoint in order to purposely mystify the reader
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