206 research outputs found

    Reep, M.

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    A Combat Artist in World War II

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    Many artists have fought in wars, and renowned painters have recorded heroic scenes of great battles, but those works were usually done long after the battles were waged. Artists have also been commissioned to visit, briefly, war-torn areas and make notes of the devastation and horror. Yet few artists who were members of any armed services have drawn or painted daily while they fought alongside their comrades. Edward Reep, as an official combat artist in World War II, painted and sketched while the battles of the Italian campaign raged around him. He was shelled, mortared, and strafed. At Monte Cassino, the earth trembled as he attempted to paint the historic bombing of that magnificent abbey. Later, racing into Milan with armed partisans on the fenders of his Jeep, he saw the bodies of Mussolini and his beautiful mistress cut down from the gas station where they had been hanged by their heels. That same day he witnessed at first hand the spectacle of a large German army force holed up in a high-rise office tower, waiting for the chance to surrender to the proper American brass for fear of falling into the hands of the vengeful partisans. Reep\u27s recollections of such desperate days are made more memorable in Combat Artist by the many painfully vivid paintings and drawings that accompany the text. Reep\u27s battlefield drawings show us, with unrelenting honesty, the horrors and griefs—and the bitter comedy—of that war fought to end wars that only spawned more. Edward Reep is professor of painting emeritus at East Carolina University and the author of a number of books, including The Content of Watercolor.https://uknowledge.uky.edu/upk_military_history/1012/thumbnail.jp

    Representative FACS histograms and REEP gating strategy.

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    <p>HEK293A cells were co-transfected with HA-α2A or -α2C ARs and control vector, Flag-REEP1, -REEP2, or –REEP6 cDNAs. Forty-eight hrs post-transfection, relative expression levels of each receptor were determined in non-permeabilized (Surface) and permeabilized (Total) cells by using a FACS assay. α2 ARs and REEPs were labeled with FITC-conjugated anti-HA and Cy3-conjugated anti-Flag (M2) antibodies respectively. <b>A</b>. Representative α2A AR FACS fluorescence distributions under non-permeabilized (Top) and permeabilized (Bottom) conditions (UT = untransfected). <b>B</b>. Representative α2C AR FACS fluorescence distributions under non-permeabilized (Top) and permeabilized (Bottom) conditions (UT = untransfected). Note the shift to higher median fluorescence upon permeabilization, which is greater for α2C vs. α2A ARs due to the larger pool of intracellular α2C ARs [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076366#B25" target="_blank">25</a>]. <b>C</b>. Representative gating strategy for FACS analysis of co-expressed α2 ARs and REEPs. Background staining of non-transfected HEK293A cells with FITC-conjugated anti-HA and Cy3-conjugated anti-Flag (M2) antibodies was determined (Top) and used to set the FACS gating thresholds for background fluorescence (Q4). Representative α2A AR and REEP1 FACS data set demonstrating co-expression of both proteins is shown (Bottom). All cells contained in quadrants Q1-3 were analyzed for calculation of REEP effects on co-expressed α2 AR surface and intracellular expression (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076366#pone-0076366-t004" target="_blank">Table <b>4A</b></a>). Data summarized from between five and eight different transfections for each combination of α2 AR and REEP with a minimum of 1000 cells analyzed for each transfection.</p

    REEP : data-centric, energy-efficient and reliable routing protocol for wireless sensor networks

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    Owing to the growing demand for low-cost 'networkable' sensors in conjunction with recent developments of micro-electro mechanical system (MEMS) and radio frequency (RF) technology, new sensors come with advanced functionalities for processing and communication. Since these nodes are normally very small and powered with irreplaceable batteries, efficient use of energy is paramount and one of the most challenging tasks in designing wireless sensor networks (WSN). A new energy-aware WSN routing protocol, reliable and energy efficient protocol (REEP), which is proposed, makes sensor nodes establish more reliable and energy-efficient paths for data transmission. The performance of REEP has been evaluated under different scenarios, and has been found to be superior to the popular data-centric routing protocol, directed-diffusion (DD) (discussed by Intanagonwiwat et al. in 'Directed diffusion for wireless sensor networking' IEEE/ACM Trans. Netw., 2003, 11(1), pp. 2-16), used as the benchmark

    Identification of α2 AR and REEP expression in permeabilized and non-permeabilized cells.

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    <p>HEK293A cells were transfected with HA-α2A AR, HA-α2C AR, Flag-REEP1, Flag-REEP2, or Flag–REEP6 cDNA. Forty-eight hrs post-transfections, cells were fixed with 4% PFA, under permeabilized and non-permeabilized conditions, and examined by wide field immunofluorescent microscopy. HA-α2 ARs were labeled with 16B12-conjugated FITC (anti-HA) antisera and Flag-REEPs were identified by M2-conjugated Alexa 594 (anti-Flag) antisera. <b>Permeabilized</b>: α2 AR staining of permeabilized cells demonstrated plasma membrane and intracellular fluorescence. Note predominant plasma membrane staining, compared to intracellular staining, consistent with efficient plasma membrane trafficking of α2A ARs. α2C AR staining revealed predominant intracellular staining with a perinuclear shadow, due their predominant localization within the ER when expressed in HEK293A cells (25). REEP staining of permeabilized cells showed strong intracellular localization, due to their ER localization. <b>Non-permeabilized</b>: α2A ARs demonstrated extensive staining of the plasma membrane, however, α2C ARs showed staining only of the plasma membrane, demonstrating that the extracellular HA epitope was not accessible by anti-HA antibody under non-permeabilized conditions. Intracellular REEP staining was similar when performed under permeabilized and non-permeabilized conditions, demonstrating that the carboxyl terminal Flag epitope was accessible by anti-Flag antibody under either condition, due to its cytoplasmic localization. REEP staining of non-permeabilized cells revealed intracellular staining, due to the ability of antibodies to gain intracellular entry following PFA fixation [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076366#B27" target="_blank">27</a>]. Representative of three separate transfections. Scale bars: 10 µm.</p

    KERAGAMAN HARD CORAL PADA HABITAT MANGROVE DAN REEP DI KAMPUNG SAWINGGRAI KABUPATEN RAJA AMPAT PAPUA BARAT

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    Penelitian ini bertujuan mengetahui keanekaragaman spesis terumbu karang khususnya Karang yang keras (Hard coral) dan mengetahui bagaimana keberadaan jenis-jenis coral reef khususnya Hard coral yang terjadi pada kedua habitat dekat hutan Manggrove dan habitat reep di kampung Sawinggrai. Metode yang digunakan adalah Transek yang dibuat sebanyak 2 transek yaitu 1 transek untuk habitat Manggrove (Hutan Bakau) dan 1 transek untuk habitat Reep, dengan ukuran transek masing-masing 5x50 m yang terdiri dari 10 plot pengamatan dengan ukuran plot 5mx5m selanjutnya Pengamatan terumbu karang per plot dengan cara snorkeling yang dikerjakan dari plot 1 ke plot 10 pada masing-masing habitat. Kegiatan yang dilakukan pada saat pengamatan antara lain: Pencatatan jumlah jenis karang keras (Hard coral) , Pemotretan, Pembuatan video. Pengambilan data ekologi berupa suhu air,pH air, Ketinggian air, Salinitas, Oksigen terlarut (Do disolved oksigen). Kegiatan Pemotretan dan pembuatan Video dibuat untuk dapat melakukan identifikasi berdasarkan foto dan yang nantinya akan dicocokan dengan gambar yang terdapat pada buku acuan yang mengacu pada: Allen dan Steene (1994) Indo-Pacific Coral Reef, Field Guide. Penerbit Tropical Reef Research, Singapore.Dari penelitian ini teridentifikasi 4 suku hard coral yang ditemukan di kawasan manggrove yang terdiri dari 8 jenis anggota Acroporidae (Acropora humilis, Acropora digitifera, Acropora Formosa, Acropora grandis, Acropora elegantula, Acropora gemmifera, Montipora tuberculosa, Montipora digitata; 1 jenis suku Agariciidae (Pavona Venos); 2 anggota Faviidae (Platygyra sinensisdan Cyphastrea microphthalma) dan 1 jenis dari anggota Pacillopolidae (Pocillopora woodjonesi). Pada Kawasan reef teridentifikasi 5 suku hard coral yang ditemukan yang terdiri dari 10 anggota Acroporidae (Acropora acuminate, Acropora hyacinthus, Acroporahumilis, Acropora palifera, Acropora digitifera, Acropora Formosa, Acropora grandis, Acropora robusta, Montifora aquituberculatadan Montipora danae); 1 anggota Dendrophylidae (Turbinaria reniformis); 6 anggota Faviidae (Favites pentagona, Favites complanata, Diplostrea heliopora, Montastrea magnistellatadan Goniastrea australiensis); 3 anggota Fungidae (Fungia repandaFungia fungitesdan Fungia moluccensis); anggota Mossidae (Symphyllia agarica) dan 1 anggota Poritidae (Alveopora spongiosa). Pada kedua kawasan baik manggrove maupun reef terlihat suku Acroporidae paling banyak ditemukan dengan kebergaman yangmpaling baaik terdapat pada kawsan reff dipengaruhi oleh beberapa faktor yaitu oksigen terlarut (DO), pH, keadaan substrat, keadaan arus serta aktivitas manusia. Kata Kunci : keanekaragaman hard coral, habitat maggrove dan reep, sawingrai raja ampa

    Breda Smalle Reep e.o. Riool Gemeente Breda Archeologische Begeleiding (conform protocol Opgraven)

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    In opdracht van de gemeente Breda heeft team Archeologie en Archief van de gemeente Breda een Archeologische Begeleiding (conform protocol Opgraven) uitgevoerd binnen het trac&#233; Smalle Reep, Klaverweide, Koele Mei en Zusterveld te Breda. Aanleiding voor het onderzoek is de aanleg van een riool trac&#233;, waarbij bodemverstorende werkzaamheden zullen plaatsvinden. De vraagstelling van dit onderzoek betreft het toetsen van de archeologische verwachting zoals verwoord in het PvE. Het veldonderzoek richt zich op het documenteren van alle vindplaatsen en vondstcomplexen die in het plangebied aanwezig zijn. Uit het onderzoek is gebleken dat er een sloot binnen het plangebied aanwezig is. Het betreft hier een ontginningssloot van het Voortbos, daterend vanaf 1332 of later. In de periode van 9 maart t/m 19 oktober 2022 zijn de rioolwerkzaamheden in het trac&#233; van de Smalle Reep, Koele Mei, Klaverweide en Zusterveld te Breda archeologisch begeleid. Hierbij is regelmatig, ook in overleg met de uitvoerder, ter plaatse gekeken bij de werkzaamheden. Het ontgraven trac&#233; is gefotografeerd en onderzocht op de aanwezigheid van archeologische sporen. Er zijn verspreid in dit trac&#233; profielen gedocumenteerd om de bodemopbouw in het plangebied in kaart te brengen. In mei is er een spoor waargenomen, namelijk een sloot. Het spoor is gedocumenteerd in het profiel, gefotografeerd en getekend. De archeologische begeleiding is uitgevoerd conform BRL 4000, protocol BRL 4004 versie 4.1 en het PvE 2022-15. De profielen tonen dat de C-horizont (S950) binnen het gehele riooltrac&#233; direct wordt afgedekt door een moderne ophogingslaag, bestaande uit bruingrijs tot wit/ licht grijs zwak siltig bouwzand (S999). Hierin kwam fijn grind voor. De C-horizont bestaat uit lichtgrijs tot witgeel zwak tot matig siltig zand. Dit is slecht gesorteerd. In delen van het plangebied is deze afzetting verspoeld. Het dekzandniveau stijgt vanuit het oosten richting het (noord)westen. Het lager gelegen deel ligt ter hoogte van moerengebied de Hoge Vucht met in het zuiden het beekdal De Loop &#8211; Watervliet. Het dekzand ligt hoger richting de dekzandrug van de Bredase Aard. De natuurlijke bodem bevindt zich op 50 tot 100 cm beneden het maaiveld (0.50 - 1.20 m + NAP). Tijdens de archeologische begeleiding van de rioolwerkzaamheden zijn vijf spoornummers uitgedeeld. Het gaat hier om &#233;&#233;n sloot (S001) en bodemlagen (S950, S951, S991 en S999). De bodemlagen zijn hierboven reeds besproken. S001 betreft een sloot. Deze sloot is aangetroffen in het westen van de Smalle Reep en kent een noord-zuid ori&#235;ntering. Vermoedelijk betreft het een ontginningssloot. Deze sloot ligt ter hoogte van &#233;&#233;n van de ontginningsstroken behorend bij de ontginning van het Voortbos. Deze sloot is ook zichtbaar op de kadastrale minuut van 1824. Sloot S001 bestaat uit een viertal vullingen. Vulling 1 bestaat uit grijsgeel, matig siltig zand, waarin lichte gleyverschijnselen voorkomen. Hieronder bevindt zich een grijze zwak zandige kleilaag met onderin gelaagde zandbandjes en wortelresten (vulling 2). Vulling 3 bestaat uit lichtgrijs tot wit gelaagd matig siltig zand. De laatste vulling reikt tot aan de onderkant van de sloot en bestaat uit een donkerbruin zandig humeus pakket, waarin donkergrijs matig siltig zand en zwakzandige klei gelaagd aanwezig zijn. Ook komen er in dit pakket plantenresten en wortels voor

    Amino acid comparison of REEP Families.

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    <div><p>The REEP family can be subdivided into two subfamilies, REEP1-4, and REEP5-6. Yeast Yop1 is most similar to the latter subfamily and has been included in the alignment. Residues completely conserved in each subfamily are highlighted in yellow, whereas partially similar residues are highlighted in blue (consensus residue derived from a block of similar residues at a given position) or green (consensus residue derived from the occurrence of greater than 50% of a single residue at a given position). Hydrophobic segments are boxed in black. The conserved 14-3-3 binding site (RSXpS) found in REEP1-4 is boxed in red. Conserved positively charged residues postulated to be involved with microtubulin binding in REEP1-4 are demarcated (*), whereas conserved negatively charged residues in REEP5-6/Yop1 are also shown (#) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076366#B34" target="_blank">34</a>]. Alignment performed using Vector NTI v.11 (Invitrogen). GenBank protein accession numbers utilized were:</p> <p>mouse REEP1 (Yip2a): NP_848723 </p> <p>mouse REEP2 (Yip2d): NP_659114 </p> <p>mouse REEP3 (Yip2b): NP_848721 </p> <p>mouse REEP4 (Yip2c): NP_850919 </p> <p>mouse REEP5 (Yip2e): NP_031900 </p> <p>mouse REEP6 (Yip2f): NP_647453</p> <p>Yop1: NP_0153</p></div

    Environmental Regulations and the Welfare Effects of Job Layoffs in the United States: A Spatial Approach

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    abstract: This article develops welfare-consistent measures of the employment effects of environmental regulation. Our analysis is based on a microeconomic model of how households with heterogeneous preferences and skills decide where to live and work. We use the model to examine how job loss and unemployment would affect workers in Northern California. Our stylized simulations produce earnings losses that are consistent with empirical evidence. They also produce two new insights. First, we find that earnings losses are sensitive to business cycle conditions. Second, we find that earnings losses may substantially understate welfare losses once we account for the fact that workers may have to commute further or live in a less desirable community after losing a job.This is a pre-copy-editing, author-produced PDF of an article accepted for publication in REVIEW OF ENVIRONMENTAL ECONOMICS AND POLICY following peer review. The definitive publisher-authenticated version Kuminoff, Nicolai V., Schoellman, Todd, & Timminsy, Christopher (2015). Environmental Regulations and the Welfare Effects of Job Layoffs in the United States: A Spatial Approach. REVIEW OF ENVIRONMENTAL ECONOMICS AND POLICY, 9(2), 198-218. http://dx.doi.org/10.1093/reep/rev006 is available online at: http://dx.doi.org/10.1093/reep/rev00
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