510 research outputs found
sj-docx-1-pie-10.1177_09544089211037443 - Supplemental material for Multi-response modeling for sliding wear behavior of AA5052/TiC composites by stir casting: A comparative analysis using response surface methodology and fuzzy logic system
No full textSupplemental material, sj-docx-1-pie-10.1177_09544089211037443 for Multi-response modeling for sliding wear behavior of AA5052/TiC composites by stir casting: A comparative analysis using response surface methodology and fuzzy logic system by Priyaranjan Samal, Pandu R Vundavilli, Arabinda Meher and Manas M Mahapatra in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering</p
Biochemical and genetic studies of wild type and attenuated vaccine strains of bluetongue virus
No full textTypescript (photocopy).A biochemical, and genetic examination of the tetravalent, attenuated vaccine for bluetongue virus (BTV) developed at Texas A&M was undertaken. These studies were aimed at (i) identifying reliable vaccine-associated markers, (ii) determining the genetic stability of the vaccine, and (iii) examining the molecular basis of attenuation in BTV. Comparative growth studies showed that maximal yields of all strains were obtained when cell monolayers were harvested early after the onset of cytopathic effects. One-step growth curves showed an altered growth kinetics for type 13 V. The four V strains were temperature-sensitive (ts) for growth at 39�� and for plaque formation at 37��. Recombination tests indicated that the ts lesions of the vaccine strains were on different reassorting genome segments. Electrophoretic comparisons of the genome RNAs, proteins, and protease digests of individual proteins species from homotypic W:V pairs revealed numerous differences. Only changes in segment 2 and its product were seen in all four W:V pairs. Changes in genome segment 2 of 10V, 11V, and 13V were detected by both direct electrophoretic analysis of genomic RNAs and partial protease analyses of the segment 2 gene product. Changes in genome segment 2 of 17V could only be inferred from the heat lability of the segment 2 product. Analyses of mixed infections between heterotypic BTV strains revealed that reassortment of genome segments between V strains and W strains, occurred at high frequency. These analyses also allowed the tentative assignment of the ts lesions of 10V and 17V to genome segments 4 and 5, respectively. This study has yielded significant results relative to the original aims. Several differences in the biological and biochemical properties of the vaccine strains that may serve as reliable vaccine markers were identified. The results of this study have been used to construct the hypothesis that mutations in genome segment 2 of the vaccine strains are responsible for the attenuated phenotype of the vaccine. If the hypothesis that attenuating mutations are located in genome segment 2 of each vaccine strain is correct, the tetravalent attenuated vaccine will be genetically stable. Finally, these results suggest that genome segment 2 of BTV encodes a product that is important in determining the virulence phenotype of the virus
Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins
No full textBACKGROUND: CtBP1 and CtBP2 are transcriptional co-repressors that modulate the activity of a large number of transcriptional repressors via the recruitment of chromatin modifiers. Many CtBP-regulated proteins are involved in pathways associated with tumorigenesis, including TGF-beta and Wnt signalling pathways and cell cycle regulators such as RB/p130 and HDM2, as well as adenovirus E1A. CtBP1 and CtBP2 are highly similar proteins, although evidence is emerging that their activity can be differentially regulated, particularly through the control of their subcellular localisation. CtBP2s from diverse species contain a unique N-terminus, absent in CtBP1 that plays a key role in controlling the nuclear-cytoplasmic distribution of the protein.RESULTS: Here we show that amino acids (a.a.) 4-14 of CtBP2 direct CtBP2 into an almost exclusively nuclear distribution in cell lines of diverse origins. Whilst this sequence contains similarity to known nuclear localisation motifs, it cannot drive nuclear localisation of a heterologous protein, but rather has been shown to function as a p300 acetyltransferase-dependent nuclear retention sequence. Here we define the region of CtBP2 required to co-operate with a.a. 4-14 to promote CtBP2 nuclear accumulation as being within a.a. 1-119. In addition, we show that a.a. 120-445 of CtBP2 can also promote CtBP2 nuclear accumulation, independently of a.a. 4-14. Finally, CtBP1 and CtBP2 can form heterodimers, and we show that the interaction with CtBP2 is one mechanism whereby CtBP1 can be recruited to the nucleus.CONCLUSION: Together, these findings represent key distinctions in the regulation of the functions of CtBP family members that may have important implications as to their roles in development, and cell differentiation and survival.<br/
Low‐mass young stellar population and star formation history of the cluster IC 1805 in theW4 HII region
No full textInternational audienceW4 is a giant HII region ionized by the OB stars of the cluster IC 1805. The HII region/cluster complex has been a subject of numerous investigations as it is an excellent laboratory for studying the feedback effect of massive stars on the surrounding region. However, the low‐mass stellar content of the cluster IC 1805 remains poorly studied till now. With the aim to unravel the low‐mass stellar population of the cluster, we present the results of amultiwavelength study based on deep optical data obtained with the Canada‐France‐Hawaii Telescope, infrared data from Two Micron All Sky Survey and Spitzer Space Telescope and X‐ray data from Chandra Space Telescope. The present optical data set is complete enough to detect stars down to 0.2 M‐circle dot, which is the deepest optical observation so far for the cluster. We identified 384 candidate young stellar objects (YSOs; 101 Class I/II and 283 Class III) within the cluster using various colour‐colour and colour‐magnitude diagrams. We inferred the mean age of the identified YSOs to be similar to 2.5 Myr and mass in the range 0.3‐2.5 M‐circle dot. The mass function of our YSO sample has a power‐law index of ‐1.23 +/‐ 0.23, close to the Salpeter value (‐1.35), and consistent with those of other star‐forming complexes. We explored the disc evolution of the cluster members and found that the disc‐less sources are relatively older compared to the disc bearing YSO candidates. We examined the effect of high‐mass stars on the circumstellar discs and within uncertainties, the influence of massive stars on the disc fraction seems to be insignificant. We also studied the spatial correlation of the YSOs with the distribution of gas and dust of the complex to conclude that IC 1805 would have formed in a large filamentary cloud
Age, size, and position of H ii ii regions in the Galaxy: Expansion of ionized gas in turbulent molecular clouds
No full textAims. This work aims to improve the current understanding of the interaction between Hii ii regions and turbulent molecular clouds. We propose a new method to determine the age of a large sample of OB associations by investigating the development of their associated Hii ii regions in the surrounding turbulent medium. Methods. Using analytical solutions, one-dimensional (1D), and three-dimensional (3D) simulations, we constrained the expansion of the ionized bubble depending on the turbulence level of the parent molecular cloud. A grid of 1D simulations was then computed in order to build isochrone curves for Hii ii regions in a pressure-size diagram. This grid of models allowed us to date a large sample of OB associations that we obtained from the Hii ii Region Discovery Survey (HRDS). Results. Analytical solutions and numerical simulations showed that the expansion of Hii ii regions is slowed down by the turbulence up to the point where the pressure of the ionized gas is in a quasi-equilibrium with the turbulent ram pressure. Based on this result, we built a grid of 1D models of the expansion of Hii ii regions in a profile based on Larson's laws. We take the 3D turbulence into account with an effective 1D temperature profile. The ages estimated by the isochrones of this grid agree well with literature values of well known regions such as Rosette, RCW 36, RCW 79, and Mii 16. We thus propose that this method can be used to find ages of young OB associations through the Galaxy and also in nearby extra-galactic sources. © 2014 ESO
Local Narratives on the Adoption of Mango Integrated Crop Management Technologies in Samal Island, Philippines
No full textThe adoption of agricultural technologies is imperative in improving the performance of the local agriculture sector in the Philippines. Numerous technology-based development projects have been implemented across the country. The Mango ICM project was one of these interventions. It was carried out in the Island Garden of Samal, Davao del Norte (IGaCoS) with the objective of supporting the local mango industry through the promotion of environmentally sustainable technologies. Years since project completion, challenges have been raised in relation to technology adoption. To understand the adoption dynamics in IGaCoS, this study dissects the complex nature of the Mango ICM technologies and the perception and reception of the local producers towards them. This study is grounded on the narratives gathered through a series of in-depth interviews with key informants and local mango producers. Results show that although the potential benefits of the extended technologies were recognized, the existing local labor and market arrangements created barriers to technology adoption. The nature of technology, market arrangement, and financial constraints were the identified reasons for the non-adoption of the local mango producer
Local Narratives on the Adoption of Mango Integrated Crop Management Technologies in Samal Island, Philippines
No full textThe adoption of agricultural technologies is imperative in improving the performance of the local agriculture sector in the Philippines. Numerous technology-based development projects have been implemented across the country. The Mango ICM project was one of these interventions. It was carried out in the Island Garden of Samal, Davao del Norte (IGaCoS) with the objective of supporting the local mango industry through the promotion of environmentally sustainable technologies. Years since project completion, challenges have been raised in relation to technology adoption. To understand the adoption dynamics in IGaCoS, this study dissects the complex nature of the Mango ICM technologies and the perception and reception of the local producers towards them. This study is grounded on the narratives gathered through a series of in-depth interviews with key informants and local mango producers. Results show that although the potential benefits of the extended technologies were recognized, the existing local labor and market arrangements created barriers to technology adoption. The nature of technology, market arrangement, and financial constraints were the identified reasons for the non-adoption of the local mango producer
When Censorship Produces. Exemplified on Zakázané prózy by Vladimír Mináč
No full textThe article uses a collection of stories by Vladimír Mináč titled Z nedávnych čias /Of the Recent Times/ banned in 1957 by Head Office of Print Media Supervision as an example so as to show productive effects of censorship. The author develops the ideas of the methodological movement New Censorship (Richard R. Burt) and shows different kinds of meaning that the censorship case produced. The author claims that the censorship interventions made in 1957 are associated with various forms of fetishism, which followed the recent publishing of the book by Vladimír Mináč Zakázané prózy (Banned proses, Ed. Pavel Matejovič, Bratislava : Slovenské informačné centrum 2015) and engaged the work of art that would be otherwise left forgotten in literary communication. Special attention is paid to the legitimizing and cannonizing effects that were prompted by the censorship
Dynamic Behavior of Fibre Reinforced Sand
No full textFibre reinforcement has more or less established itself as a composite civil engineering material having significant effect in improving the static strength characteristics of granular soil like sand. However the dynamic behavior of fibre reinforced sand as far as large scale model tests are concerned is rarely discussed in the literature. The present study illustrates the effect of randomly distributed polypropylene fibrillated fibre reinforcement in modifying the dynamic characteristics of locally available sand (Solani River, Roorkee, India). Block resonance tests (BRT) for low-medium strain levels and Cyclic plate load tests (CPLT) for high strain level have been conducted both on un-reinforced and fiber reinforced sand in the laboratory to study its dynamic characteristics. In BRT, the magnitude of resonant frequency and maximum amplitude of vibration of the test block have been recorded at different excitation levels in vertical mode of vibration at a particular percentage of fiber content, thus establishing the effect of dynamic loading or in turn the effect of strain level. The dynamic response was evaluated in terms of coefficient of elastic uniform compression and damping ratio. Where as, in CPLT, settlement and pressure values have been recorded to calculate coefficient of elastic uniform compression at two different strain levels. It was observed that there was no significant effect of fibre reinforcement on the dynamic behavior of sand contrary to the static loading condition
- …
