56 research outputs found
Supplemental Material, Appendix_A_SAP - Seizure Action Plans for Pediatric Patients With Epilepsy: A Randomized Controlled Trial
No full textSupplemental Material, Appendix_A_SAP for Seizure Action Plans for Pediatric Patients With Epilepsy: A Randomized Controlled Trial by Dara V.F. Albert, Jennifer J. Moreland, Ann Salvator, Melissa Moore-Clingenpeel, Babitha Haridas, Justin W. Cole, Peter Glynn, Marci Fults, M. Zachary Dawson, Patty Moreland and Anup D. Patel in Journal of Child Neurology</p
Supplemental Material, Appendix_B_MIF - Seizure Action Plans for Pediatric Patients With Epilepsy: A Randomized Controlled Trial
No full textSupplemental Material, Appendix_B_MIF for Seizure Action Plans for Pediatric Patients With Epilepsy: A Randomized Controlled Trial by Dara V.F. Albert, Jennifer J. Moreland, Ann Salvator, Melissa Moore-Clingenpeel, Babitha Haridas, Justin W. Cole, Peter Glynn, Marci Fults, M. Zachary Dawson, Patty Moreland and Anup D. Patel in Journal of Child Neurology</p
Antioxidant and hepatoprotective effects of flower extract of Millingtonia hortensis Linn. on carbon tetrachloride induced hepatotoxicity
No full textObjective: Millingtonia hortensis Linn is an abundant resource of flavonoids, which might be beneficial in protecting liver tissue from injury. The hepatoprotective and antioxidant potential of ethanolic extract of M. hortensis on carbon tetrachloride (CCl 4 ) induced hepatotoxicity and the possible mechanism involved therein were investigated in rats. Materials and Methods: Preliminary phytochemical studies were carried out to determine the total phenol and flavonoid contents. 30 adult Wistar rats were allocated into 5 groups. Control group received vehicle, group-2 received CCl 4 alone (1 ml/kg body weight, intraperitonially), groups 3 - 5 received the ethanolic flower extract in 2 dose levels (200 and 400 mg/kg) and Curcumin (100 mg/kg) as a standard for 8 days orally, followed by CCl 4 as a single dose on the 8 th day. 48 hours later, blood was withdrawn, serum was subjected to biochemical assessments, and liver homogenate was examined for lipid peroxides, glutathione, superoxide dismutase, catalase and total protein levels. Furthermore, hepatic tissues were subjected to histopatological studies. Results: CCl 4 treatment produced a profound increase in the levels of malondialdehyde, hepatic marker enzymes and bilirubin content compared with the control (P < 0.05). Pre-treatment with the flower extract of M. hortensis significantly enhanced the levels of endogenous antioxidants and reduced the levels of hepatic marker enzymes in relation to the CCl 4 treated group (P < 0.05). Balloning degeneration and fatty changes in hepatocytes was prevented by pre-treatment with the flower extract. Conclusion: The antioxidant nature of the flower extract of M. hortensis could be responsible for averting damage to the liver
Not Available
No full textNot AvailableAn experimental trial was conducted for 90 days toevaluate the growth performance, immunophysio-logical response of GIFT strain of Tilapia in biofloc-based rearing system and to assess the relativepercentage survival in 3 days after challenging withthe virulent strain ofAeromonas hydrophila.Finger-lings with an average body weight 0.98 0.06 gwere stocked in triplicate at different stocking densi-ties of 200 (SD1), 250 (SD2), 300 (SD3) and 350(SD4) m 3in biofloc-based treatments and 150(C) m 3in control (clear water). Biofloc-based units(SD1 and SD2) obtained significantly better(P<0.05) growth performances at the end of theexperimental period. Mean body weight of fish in bio-floc-based units showed a decreasing trend withincrease in stocking density with 100% survival inall units including control. The stress parameterswere significantly lower in biofloc-based rearingunits especially in treatments SD1 and SD2 as com-pared to the control. The fish from the biofloc-basedunits (SD1 and SD2) possessed significantly(P<0.05) higher immune status as compared tocontrol and other biofloc treatments in terms of respi-ratory burst, serum lysozyme and myeloperoxidaseactivity. Relative survival percentages were signifi-cantly better in biofloc treatments with highest inSD1 and SD2 (83.33%) after challenge study.GIFTstrain of Tilapia at higher stocking densities 200–250 nos m 3can be taken as optimum stockingdensity whereas higher stocking densities up to350 nos m 3can be reared in the biofloc systemswithout compromising the growth and immunity.Not Availabl
Differential induction of Superoxide Dismutase in Downy Mildew-resistant and Susceptible Genotypes of Pearl Millet
No full textDifferential induction of superoxide dismutase (SOD) in downy mildew-resistant and -susceptible genotypes of pearl
millet (
Pennisetum glaucum
) was observed on inoculation with
Sclerospora graminicola
. SOD activity was studied in
resistant (IP18292) and susceptible (23B) pearl millet seedlings inoculated with
S. graminicola
. SOD activity increased by
2·3-fold in resistant seedlings upon inoculation. SOD activity was greatest in roots, with a specific activity of 3182 U per mg
protein, after inoculation. SOD activity increased in all the resistant genotypes upon inoculation with
S. graminicola
.
Native PAGE analysis showed four isozymes of SOD, three of which (SOD-1, -2 and -4) were Cu/Zn-SOD, whereas
isozyme SOD-3 was Mn-SOD. This study also revealed increased intensity of all four isozymes of SOD in the resistant
genotype upon inoculation. The involvement of SOD in pearl millet (host)–downy mildew pathogen interaction is
discussed
Not Available
No full textNot AvailableAn experimental trial was conducted for 90 days to evaluate the growth performance, immunophysiological response of GIFT strain of Tilapia in biofloc‐based rearing system and to assess the relative percentage survival in 3 days after challenging with the virulent strain of Aeromonas hydrophila. Fingerlings with an average body weight 0.98 ± 0.06 g were stocked in triplicate at different stocking densities of 200 (SD1), 250 (SD2), 300 (SD3) and 350 (SD4) m−3 in biofloc‐based treatments and 150 (C) m−3 in control (clear water). Biofloc‐based units (SD1 and SD2) obtained significantly better (P < 0.05) growth performances at the end of the experimental period. Mean body weight of fish in biofloc‐based units showed a decreasing trend with increase in stocking density with 100% survival in all units including control. The stress parameters were significantly lower in biofloc‐based rearing units especially in treatments SD1 and SD2 as compared to the control. The fish from the biofloc‐based units (SD1 and SD2) possessed significantly (P < 0.05) higher immune status as compared to control and other biofloc treatments in terms of respiratory burst, serum lysozyme and myeloperoxidase activity. Relative survival percentages were significantly better in biofloc treatments with highest in SD1 and SD2 (83.33%) after challenge study. GIFT strain of Tilapia at higher stocking densities 200–250 nos m−3 can be taken as optimum stocking density whereas higher stocking densities up to 350 nos m−3 can be reared in the biofloc systems without compromising the growth and immunity.Not Availabl
Not Available
No full textNot AvailableDynamics of claw development in Macrobrachium rosenbergii (de Man, 1879) was evaluated through monosex culture. The segregated males and females were stocked separately in two earthen ponds of 200 m2 area, at a density of 2.5 m-2 and reared for
3 months. Percentage contribution of claw weight to body weight (PCB) increased from 8.96 to 14.4% in the first month, but the change was minimal (14.4 to 17.19%) during the rest of the culture period. In order to delineate the relationship further, the data obtained was pooled together and classified into different classes based on the body weight (class interval
10 g). Interestingly, PCB in males decreased gradually upto 30 - 40 g weight class and then increased considerably for higher weight classes. But for females, the increase in PCB was marginal.Not Availabl
Evaluation of claw development in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879)
No full textDynamics of claw development in Macrobrachium rosenbergii (de Man, 1879) was evaluated through monosex culture. The segregated males and females were stocked separately in two earthen ponds of 200 m2 area, at a density of 2.5 m-2 and reared for
3 months. Percentage contribution of claw weight to body weight (PCB) increased from 8.96 to 14.4% in the first month, but the change was minimal (14.4 to 17.19%) during the rest of the culture period. In order to delineate the relationship further, the data obtained was pooled together and classified into different classes based on the body weight (class interval
10 g). Interestingly, PCB in males decreased gradually upto 30 - 40 g weight class and then increased considerably for higher weight classes. But for females, the increase in PCB was marginal
Induction of Lipoxygenase in downy mildew resistant seedlings of pearl millet in response to inoculation with Sclerospora graminicola
No full textLipoxygenase (LOX) activity was studied in seedlings of pearl millet genotypes resistant and susceptible to downy mildew pathogen Sclerospora graminicola. An increase in LOX activity was observed during the incompatible host-pathogen interaction whereas the activity decreased in compatible ones. Resistant pearl millet seedlings exhibited a 2.4-fold increase in LOX activity after inoculation with the pathogen. The enzyme activity was maximum at 18 h after inoculation. The enzyme activity was maximum in shoot portion of resistant genotype after inoculation. The enzyme activity correlated well with the degree of host-resistance to the pathogen. Substrate-based gel assay revealed five isozymes (LOX-1, LOX-2, LOX-3, LOX-4 & LOX-5) in both susceptible and resistant genotype. An additional isozyme (LOX-6) was unique to only resistant genotype after inoculation
Purification and partial characterization of manganese superoxide dismutase from downy mildew resistant pearl millet seedlings inoculated with Sclerospora graminicola
No full textThe Mn-SOD was purified from pearl millet by ammonium sulfate precipitation followed by column chromatography using DEAE-cellulose and Sephadex G-100. The isozyme has a molecular weight of 35 kDa. Electrophoresis revealed a single band of SOD activity corresponding to the purified enzyme. The purified enzyme was stable over a pH range of 7.0–9.0 for 24 h, and a temperature range of 20–35°C. The purified pearl millet SOD exhibited insensitivity to hydrogen peroxide and cyanide, which is typical of Mn-containing SOD. SOD was purified 73-fold from pearl millet
- …
