37 research outputs found
Onychiurus gevi Arbea, 2012, sp. nov.
No full text<i>Onychiurus gevi</i> sp. nov. <p>Figs. 1–12</p> <p> <b>Type locality.</b> Spain, Sima Gesm, Coordinates 36º41’32’’N, 5º00’22’’W, 1752 m a.s.l. in the Sierra de las Nieves karst of Tólox (Province of Málaga).</p> <p> <b>Type material.</b> Holotype: female (mounted on slide labelled EML0017): Spain, Málaga, Tólox, Sierra de las Nieves karst, Sima Gesm, 27.VIII.2006, G.E.V. (Speleological Club of Villacarrillo) leg. Paratypes: one reproductive male, seven females (mounted on slides labelled EML0017). Holotype and paratypes deposited in The Museum of Zoology at Pamplona, University of Navarra.</p> <p> <b>Other material.</b> One female (mounted on slide labelled EML0016): Spain, Málaga, Tólox, Sierra de las Nieves karst, Sima Gesm, 15.VII.2006, G.E.V. leg. Two males and three females (mounted on slides labelled EML0034): Spain, Málaga, Tólox, Sierra de las Nieves karst, Sima de Raja Helada (TO-8), Coordinates 36º41’35’’N, 5º00’27’’W, 1747 m a.s.l., 12.X.2009, Patricia Carrasco leg. All specimens deposited in the private collection of the author.</p> <p> <b>Etymology.</b> The species is dedicated to the members of the Speleological group of Villacarrillo (G.E.V.), who collected specimens of this species from Málaga caves.</p> <p> <b>Description.</b> Colour white in alcohol. Body length (excluding antennae) of adults: holotype 2.8 mm, reproductive males 1.77–2.29 mm, reproductive females 2.27–3.02 mm. Cuticle granulation more or less uniform and fine, somewhat coarser on terga, head capsule, and around anal spines.</p> <p>Antennal bases well marked. Antennae slightly shorter than head; ratio antennae/head diagonal = 0.86. Ant. IV with subapical organite; microsensillum in latero-external position, approximately two-fifths length from the base; sensilla not well distinguishable from ordinary chaetae; invaginated apical bulb small. Ant. I, II and III with 8, 14 and 17 chaetae respectively. AIIIO as in Fig. 5, with five papillae, two small sensory rods, two bent and smooth sensory clubs, four guard chaetae, and lateral microsensillum.</p> <p>PAO consists of 16–17 finely granulated vesicles (Fig. 6). Labrum as in Fig. 7 with 5,4,2 chaetae. Labial palp of type A with five proximal chaetae; labial papillae A, B, C, D and E with 1, 4, 0, 3 and 3 guard chaetae respectively (Fig. 8); chaetotaxy of the basomedian field (submentum) with 4+4 chaetae (Fig. 8), basolateral field (mentum) with 5 chaetae (Fig. 8); outer maxillary lobe with one basal chaeta and without sublobal hairs (Fig. 8). Mandible with strong molar plate and four (rarely five) apical teeth as in Fig. 9; maxilla bearing three teeth and six lamellae as in Fig. 10.</p> <p>Dorsal pseudocellar formula 32/033/44433, ventral 11/000/0101; all subcoxa I with one pseudocellus. Parapseudocelli not visible on the body.</p> <p>Dorsal chaetotaxy as in Fig. 1 and Tables 2–4; nearly symmetrical, poorly differentiated into meso-, and microchaetae, sometimes extra chaetae and asymmetries have been observed; macrochaetae only differentiated on the two last Abd. tergites. Th. II and III with microsensilla located laterally. Body sensilla cylindrical, poorly differentiated. Head with unpaired dorsal chaeta d0. Th. I with 10–11+10–11 chaetae. Th. II to Abd. III with 4,4,3,3,3 chaetae respectively on both sides of axial line and without unpaired axial chaetae. Abd. IV and V without unpaired axial chaetae; rarely chaeta a0 present on Abd. IV. Ratio chaetae M/s = 1.4–1.5 on Abd. V. Abd. VI with 2–3 unpaired axial chaetae: (a0), m0 and p0 (Fig.11). Subcoxa I of I, II, III pairs of legs with 2,3,3 chaetae respectively. Anal spines rather short, about 0,6 of inner edge of the claw III length.</p> <p>g 9 chaetae; g4 absent</p> <p>Chaeta number/serie 1 2 3 4 5 6 7 Th I</p> <p>m - - m - m - m Ventral body chaetotaxy as in Fig. 2, with 0+0, 1+1 and 1+1 ventral chaetae on pro-, meso- and metathorax respectively. VT with 7–8+7–8 apical chaetae and 1+1 basal chaetae. Furca reduced to a finely granulated area, with 2+2 posterior setulae arranged in one row (Fig. 12). Male ventral organ absent.</p> <p>Tibiotarsi I, II, and III with 15–16(7,7–8,1), 15–16(7,7–8,1) and 15(7,7,1) chaetae respectively; distal whorl with seven chaetae (Fig. 4). Claws without teeth. Empodial appendage slender, as long as inner edge of a claw, without basal lamella (Fig. 4) (appendage length 1.0–1.1 inner edge of claw).</p> <p> <b>Ecology.</b> <i>O. gevi</i> <b>sp. nov.</b> is bisexual species. It has been collected in cold and wet cave habitats. It has been found together with <i>Deuteraphorura cebennaria</i> (Gisin, 1956) (Arbea <i>et al.</i> 2011).</p> <p> <b>Discussion.</b> <i>O. gevi</i> <b>sp. nov.</b> resembles the <i>Onychiurus obsiones</i> species group <i>sensu</i> Kaprus’ (2008). It clearly differs from all members of the group in size (1.7–3.0 in <i>O. gevi</i> <b>sp. nov.</b> versus 0.6–1.0 mm), number of pseudocelli on Abd. tergites I–III (4,4,4 in <i>O. gevi</i> <b>sp. nov.</b> versus 3,3,3), ventral pseudocellar formula (2/000/0101 in <i>O. gevi</i> <b>sp. nov.</b> versus 2/000/0001), and habitat preferences (caves in Málaga, S Spain in <i>O. gevi</i> <b>sp. nov.</b> versus soil in mountains beech forest in Poland, and sandy habitats in arid and semiarid regions of N Africa, SE Europe, Minor and Middle Asia) (see Kaprus’ 2008 and Smolis & Skarżyński 2009).</p> <p> Some <i>Onychiurus</i> species: <i>O. boldorii</i>, <i>O. nathanieli</i>, <i>O. paoletti</i>, <i>O. stillicidii</i> also share the same dorsal pseudocellar formula on head and Th. as the new species, but differ in dorsal pseudocellar formula on Abd. (4,4,4,3,3 in <i>O. gevi</i> <b>sp. nov.</b> versus 3,3,3,4, 3 in <i>O. boldorii</i> and <i>O. stillicidii</i>, 3,3,3,5,3– 4 in <i>O. nathanieli</i>, and 3,3,3,5, 3 in <i>O. paoletti</i>) and ventral pseudocellar formula (see Table 5).</p> <p>The new species is distinguished from all other species of the genus by the number of pseudocelli on Abd. I–III (see Table 5).</p>Published as part of <i>Arbea, Javier, 2012, Review of the genus Onychiurus Gervais, 1841 (Collembola: Onychiuridae) with description of a new cave species from Southern Spain, pp. 33-46 in Zootaxa 3564</i> on pages 36-43, DOI: <a href="http://zenodo.org/record/209507">10.5281/zenodo.209507</a>
Additional file 3: Figure S1. of Urinary metabolomics of young Italian autistic children supports abnormal tryptophan and purine metabolism
No full textAccurate mass and MS/MS fragmentation data for (a) kynurenine, (b) melatonin, and (c) tryptophan. (TIF 191 kb
Protonemura gevi Figueroa & López-Rodríguez, 2010, sp. n.
No full textProtonemura gevi sp. n. Tierno de Figueroa & López-Rodríguez Figs. 1–4. Type Material: Holotype: Spain, Jaén, Siles, Cueva del Nacimiento del Arroyo de San Blas (cave of the San Blas stream source), 1000 m a.s.l., coordinates: 38 º 23 ’ 12.94 ’’ N, 2 º 32 ’06.10’’ W, 60 m deep in the cave, 19 - VII-2009, 1 male, G.E.V. leg. Paratypes: same locality, date and collectors, 5 males, 6 females, 10 nymphs (5 males and 5 females). The holotype and some paratypes (2 males, 3 females, 2 male nymphs and 2 female nymphs) are deposited in the Collection of the Museo Nacional de Ciencias Naturales (Madrid, Spain) with the identification codes: Nº Cat. TIPOS MNCN 1937. The remaining paratypes (3 males, 3 females, 3 male nymphs and 3 female nymphs) are deposited in the Tierno de Figueroa’s collection in the Departamento de Biología Animal, Universidad de Granada (Granada, Spain). Other material examined: same locality, date and collectors, 9 nymphs. Type locality: Cueva del Nacimiento del Arroyo de San Blas (cave of the San Blas stream source) (Siles, Jaén, Spain). Etymology: The specific name of this taxon is in honour of the speleological group of Villacarrillo (G.E.V., Grupo Espeleológico de Villacarillo) that collected the specimens. Diagnosis. Body length: males 6.1 –8.0 mm (holotype 8.0 mm), females 6.9–8.4 mm. Forewing length: males 4.2–4.7 mm (holotype 4.4 mm), females 5.0– 5.9 mm. Habitus. Head (Fig. 1 A) brown and yellow: yellowish background, with a dark brown triangular zone among the ocelli, a central dark brown zone in the occiput, two light brown rounded areas between the occiput and the posterior ocelli, dark brown areas rounding the antennae bases and extending along the clypeuslabrum area with a small yellow zone at the frons. Compound eyes scarcely prominent. Antennae very dark except in the basal area (yellow) and very long, longer than the body length (Fig. 4). Prosternal gill remnants white, slender and short, without narrowing. Pronotum dark brown, with a yellow triangle posteriorly and light brown anterolaterally (Fig. 1 A). Meso- and metanotum yellowish at the prescutum, and brown at the scutum and scutellum. Legs yellow with brown transversal bands in the femur. Wings brachypterous in both sexes. Abdomen yellowish with the last segments brown, from the eighth segment in males and from the ninth segment in females. Cerci cylindrical with the apex narrower than the base, particularly long in some males in comparison with others. Male: Eighth abdominal tergum with one or two small black setae at each side of the central line. Ninth abdominal tergum with two posteriorly protruding lobes covered with many black setae. Hypoproct subpentagonal with its posterior apex long, finger-shaped (Fig. 2 A). Vesicle long, as long as the length of the ninth sternite. Epiproct: laterally unique, with a pronounced white hump midway on the dorsal sclerite (Fig. 2 C), apex pointed and slightly curved upwards, ventral sclerites with a rounded, spine-bearing projection; dorsally, the epiproct is long and rounded distally (Fig. 2 B); apical tubelike projection absent. Paraproct (Fig. 2 D): inner lobes not hidden by the hypoproct (Fig. 2 A); sclerotized base of median lobes subtriangular, inner branch darkly sclerotized, short and enlarged in its apex, membranous portion bulbous and covered with small hairs; outer lobes sclerotized, hammer-shaped (notably enlarged apically), slightly curved across the top of the median lobe and bearing black thick setae in the apex. Female: Pregenital plate (seventh sternum) slightly protruding over the eighth sternum and slightly sclerotized in its central part (Fig. 2 E). Subgenital plate trapezoidal, widest anteriorly, the posterior margin having a shallow emargination; the vaginal lobes well visible, extending from the corners of the subgenital plate. Paraproct rounded, slightly wider than long. Mature nymph: Body length: males 5.9 –7.0 mm, females 8.2 –10.0 mm. General colour yellowish-brown. Legs, antennae and cerci also yellowish brown but lighter. Body nearly without setae, except in the legs and the cerci, where small setae are present. Head (including eyes) approximately as wide as the pronotum (Fig. 3 A). Prosternal gills (Fig. 3 B), as in adults, white, slender and short, without narrowing. Pronotum almost rectangular, widest anteriorly (Fig. 3 A). Pronotum marginal setae continuous and short, longest at corners. Mesonotum with hairs in the proximal corners longer than those of the pronotum. Legs with dorsolateral dark setae on the femur that are 1 / 3 femur width, shorter dark setae on the tibiae mainly found on distolateral margin (Fig. 3 A&B). Abdominal segments 1–6 divided into tergum and sternum. Abdominal terga sparsely populated by small, short setae throughout the tergum, a close set row of setae at the posterior margin of each segment, paired spines lacking (Fig. 3 C). Paraprocts with sparse pilosity, triangular in ventral view, proportionally longer in the female nymph, with tip rounded in the female, truncate in the male (Fig. 3 E&D). Cerci longer than the abdomen, with short hairs in the distal margin of each article, with the first articles wider than longer (approximately until the tenth), becoming progressively longer in relation to width distally. Affinities. Morphologically, P. gevi sp. n. is clearly different from the remaining Iberian Protonemura species, and cannot be included in currently recognized groups (Vincon & Muranyi, 2009). The most similar Iberian species is P. culmenis Zwick & Vinçon, 1993 which is isolated in the Pyrenees (Vincon & Muranyi, 2009). The female pregenital plate is similar in both species, but wider in P. gevi sp. n. The epiproct of P. gevi sp. n. has a characteristic hump formed by the distal third of the ventral sclerite, not so prominent in P. culmenis. The paraprocts of males of the two species differ mainly in the shape of the inner branch of the median lobes. In P. culmenis the branch is finger-shaped, while in P. g e v i it is enlarged in its apex. The general pilosity is much scarcer in P. g e v i sp. n. than in P. culmenis. Regarding additional Protonemura species, some similarities can be found in the shape of the male paraprocts and the female subgenital plate with the North African talboti subgroup of the corsicana group (especially to P. b e r b e r i c a Vinçon & Sánchez-Ortega, 1999), but the absence of the apical tubelike projection in the epiproct of P. g e v i sp. n. indicates that this species does not belong to the corsicana group. Particularly similar to P. g e v i sp. n. is P. hassankifi Aubert, 1964 from Iran, mainly by the paraproct shape, although the epiproct does not present a hump so prominent. The subgenital plate of the P. hassankifi female is similar but without the median depression. P. gevi has scarcely prominent compound eyes in comparison with the only two other Protonemura species from Southern Iberian Peninsula [P. alcazaba (Aubert, 1954) and P. meyeri (Pictet, 1841)] (Fig. 1) and also in comparison with other Palearctic Protonemura species that we could study [P. angelieri Berthélmy, 1963, P. aroania Tierno de Figueroa & Fochetti, 2001, P. auberti Illies, 1954, P. beatensis (Despax, 1929), P. berberica, P. canigolensis Zwick & Vinçon, 1993, P. caprai (Aubert, 1954), P. consiglioi (Aubert, 1953), P. culmenis, P. globosa Berthélemy & Whytton, 1980, P. hiberiaca Aubert, 1963, P. hispanica (Aubert, 1956), P. intricata (Ris, 1902), P. lateralis (Pictet, 1835), P. navacerrada (Aubert, 1954), P. praecox (Morton, 1894), P. pyrenaica Mosely, 1930, P. ruffoi Consiglio, 1961, P. salfii (Aubert, 1954), P. talboti (Navás, 1929), P. tuberculata (Despax, 1929) and P. vandeli Berthélemy, 1963]. The P. gevi sp. n. nymph does not present, as P. nimborum (Ris, 1902), paired spines in the abdomen terga frequently found in some other Protonemura species, but the lack of many Protonemura nymph descriptions does not let us to discuss this character further. Ecological remarks. This species, both adults and nymphs, was collected at 60 m depth from the exit of the cave of the San Blas stream source (Fig. 5), an unusual habitat for a stonefly. The abiotic characteristics were: 67 % humidity, 7 ºC water temperature, 9 ºC air temperature, total absence of light, clear oligotrophic water, and substrate composed mainly by cobble and gravel, but also of sand and lime precipitate. Protonemura gevi sp. n. is abundant but probably localized in the study area. In 2002, M. Baena and A. Pérez Ruiz collected some stonefly individuals in the same cave. Unfortunately, this material was lost in the mail. After the collection of the typical series in July 2009, a new visit to this area in August 2009 by T. Pérez Fernández led to the observation of many nymphs and adults. A visit on 7 September, 2009 resulted in the observation of 4 adults and 2 mature nymphs in the cave, but these were not collected. No further collections were made because we did not know the status of the population and its restricted distribution area probably makes it threatened. The presence of mature nymphs and adults in July, August and September indicates a summer flight period for this species, but given the potentially stenothermic nature of the stream habitat, it is possible that emergence extends to other seasons. No individuals were found out of the cave and, although more samplings are needed to completely confirm it, it is possible that the distribution area of this species is limited to this cave and, perhaps, to some nearby cavities. Thus, considering the relatively good taxonomic knowledge of the stonefly fauna of the Southern Iberian Peninsula, this species could be considered a microendemic. It is usually accepted that aquatic insects inhabiting caves do not exhibit discernible modifications from a hypogean existence (Ward 1992). Nevertheless, P. g e v i sp. n. has relatively small eyes in comparison with other Protonemura species, antennae in adults are considerably long, longer than the body length, and both sexes present a notable degree of wing reduction. This last character is usually associated with populations living in high mountains or to only one of the sexes, not both. We think that this wing reduction in both sexes of P. g e v i sp. n., and small compound eyes and specially the long antennae, are probably a consequence of environmental pressures of this particular habitat.Published as part of Figueroa, José Manuel Tierno De & López-Rodríguez, Manuel Jesús, 2010, Protonemura gevi sp. n., a cavernicolous new species of stonefly (Insecta: Plecoptera), pp. 48-54 in Zootaxa 2365 on pages 48-53, DOI: 10.5281/zenodo.27576
Gene Expression Profiling as a New Real-Time Assay in Human Biomonitoring of Waste-to-Energy Plant Workers
No full textExposure to heavy metals represents one of the most important risk factors for the health of incinerator workers. Indeed, heavy metals can determine increased generation of reactive oxygen species (ROS). In this work, we introduced the use of transcription profiling of detoxifying genes, involved in redox balance and genome integrity, as a highly sensitive assay of heavy metal exposure and subsequent oxidative stress. For this purpose, blood mRNA levels of OGG1, ST13, NQO1 and MT1A genes, as well as urinary concentrations of nine heavy metals and the oxidized base 8-OHdG of 49 subjects (26 controls and 23 employees in the waste-to-energy plant of San Zeno, Arezzo, Italy) were determined. No significant difference between the two populations was observed, thus highlighting, as far as the biomarkers analysed are concerned, the absence of occupational exposure to heavy metals and systemic oxidative stress induction in the workers of the waste-to-energy plant of San Zeno. Correlation analyses underline a close association between heavy metals exposure and changes in expression levels of a number of genes, even at low exposure doses, thus remarking the greater capacity of detection of transcription profiling compared to other biomarkers and the importance of its introduction in future human biomonitoring programs
Costume development for Jõhvi`s Folk Dance Group "Gevi"
No full textDiplomitöö käigus töötati välja Jõhvi rahvatantsurühma „Gevi“ kostüümi osad, mille kampsunite aluseks on Eesti Rahva Muuseumi kogudest pärit Simuna kampsunid ja kleidi inspiratsiooniks tellija poolt esitatud pilt. Töö käigus lähtuti kliendi soovidest; individuaaltöö ning kostüümi- ja rahvarõivaste valmistamise eripäradest. Autor konstrueeris toodete lõiked ja töötas välja tehnoloogia. Töö tulemusena õmbles autor valmis ühe rahvatantsija villase- ja ruudulise kampsuni ning kleidi suuruses 42. Ülejäänud tantsijate kleidid õmmeldakse rahvatantsuseltsi poolt palgatud õmbleja poolt. Lekaalikomplektid valmisid standardsuuruste tabeli mõõtude järgi ja kohandatakse proovide käigus iga tantsija personaalsete mõõtude järgi. Lekaalide põhjal on edaspidi võimalik unifitseerida ka teiste kihelkondade kampsuneid. Kostüümi kampsunite tehnilise töötlemise viis erineb oluliselt tänapäevasest, mistõttu töödeldi paljud lõikeservad ja ääred puhtaks käsitsi. Tantsijate liikumisvabaduse saavutamiseks lisati varrukatele kaenlalapid, mis võimaldavad paremini käsi tõsta. Lisaks pidi tehniliste lahenduste loomisel arvestama tantsijate vahetumisega rühmas, mille tõttu pidid tooted olema korrigeeritavad. Diplomitöö jagunes seitsmeks peatükiks, mis on järjestatud lähtudes individuaaltööna valminud toodete valmistamisest. Töös kirjeldatakse toodete ajaloolist tausta ja inspiratsiooni, lekaalide valmistamise põhimõtteid, põhi- ja moekohaste lõigete valmistamist M. Müller & Sohn süsteemist lähtudes ning unifitseerides, lekaalide paljundamist suurustele 38-52, materjalide ja furnituuride valikut, kangakulu arvestust ning tehnoloogiat. Seitsmendas peatükis kirjeldatakse tootmiskulude arvutamist, võttes aluseks ettevõttes juurutatud meetodi. Töös kajastuvad joonised on tehtud järgmiste programmidega: Optitex, Optitex Marker ja Corel DESIGNER. Autori jaoks oli diplomitöö koostamine huvitav ja uudne kogemus. Töö eelduseks oli Eesti Rahva Muuseumis originaalesemete uurimine ja ajaloolise materjaliga tutvumine, mis tagasid põhjalikud teadmised tehtavast. Kuigi autentsuse tagamiseks tuli välja mõelda erinevaid tehnoloogilisi lahendusi, on autor tulemusega rahul. Suur abi oli diplomitöö ettevõttepoolse juhendaja teadmistest ja kogemustest, mille tõttu jäid paljud vead tegemata. Kuigi valminud lekaalide juures on aspekte, mida saab edasise arendamisega parandada, võib esimesed tooted lugeda õnnestunuks.The aim of the final thesis was to create linen dress, woolen and checked long-sleeved jacket for folk dancers group. Jackets had to be based on Simuna`s long-sleeved jackets from the collections of the Estonian National Museum. The dress was inspired of picture, which was given by customer and had to fit to all of the dancers. The author was tasked with drafting the patterns, creating technical solutions and tailoring costumes. Jõhvi`s woman`s group Gevi has been engaged with folk dance in Ida-Virumaa for 53 years. In 2001. founded non profit organization Jõhvi`s Folk Dance Association Gevi, to manage and lead group`s activities. Association`s main goal is to develope and preserve Estonian national culture. [1] Silvi Allimann, the founder of Kaunis Rahvarõivas Oü, has been engaged making folk costumes for 40 years [2]. Earlier, her company has made folk costume set for „Gevi“. Set included woolen skirt with longitudinal stripes, embroidered blouse, cap, apron, undershirt, belt, long-coat and kerchief. Folk dance costumes are different from regular garments. Their seam allowances must be longer, because costumes are fitted to each dancer and many cutting edges are processed manually. Also there is added armpit patch to give dancers freedom for movement. This graduation thesis consists of seven chapters, each of them describes different part of costume development. The first chapter describes historical long-sleeved jackets and explains why Simuna`s jackets were chosen. Also there is pointed out the changes, which were made based on dancers needs. The chapter concludes description of dress designing and clients demands for the item. The following part describes models, their details, materials and attachment. There are also technical drawings and explanations for the choice of the materials. The third chapter contains the base bodice pattern drafting according to M. Müller & Sohn method. There are also described modifications which are made for patterns and measurements which are used for constructions. The fourth paragraph covers the technical solutions and technical sectional drawings. Resolutions are described in table of technical processing. The following part reviews the development of master patterns, seam allowances and grading rules. The sixth chapter describes lay planning and calculations in material usage effiency. There are compared combined and consolidated layout. The seventh paragraph contains calculation of producton costs using the company`s method. Working on this thesis has been interesting and fresh experience. The assumption of this work was investigation of original objects in Estonian National Museum and study of historical material. These methods gave good basic knowledge. Although different technological solutions had to be made to ensure authenticity, the end result is satisfying. Company`s tutor`s knowledges and experiences were a great help in making this thesis and though master patterns have aspects which can be improved during further work, the first products can be considered successful
Comparison among plasma-derived clotting factor VIII by using monodimensional gel electrophoresis and mass spectrometry
No full textBackground. Deficiency or dysfunction of coagulation factor VIII (FVIII) is the underlying cause of haemophilia A. Haemophilic patients are at present treated with plasma-derived FVIII (pdFVIII) or recombinant FVIII (rFVIII) in order to correct their clotting deficiency. pdFVIII concentrates are exclusively produced from human plasma upon pooling from multiple donors. It is not know whether the presence of excess of other plasma proteins, in addition to von Willebrand factor, could stimulate untoward immune responses in the recipient. Thus, information regarding the presence of contaminants in commercial products is of concern. Materials and Methods. Two commercially available pdFVIII concentrates were characterized through SDS-PAGE and mass spectrometry Emoclot® and Beriate®. Results. The components of two pdFVIII products considered in this study were well identified by mass spectrometry analysis, in both cases we found abundant components coming from blood plasma, and some other contaminants. Only in Beriate® we also found truncated form of pdFVIII. Conclusion. The two pdFVIII examined showed the presence of vWF, Fibrinogen in excess, and other substances that could be considered as contaminants or impurities. © SIMTI Servizi Srl
Peningkatan Hasil Pembelajaran Lari Sprint 60 m Melalui Metode Permainan pada Siswa Kelas Atas SD Negeri 009 Teluk Kabupaten Pelalawan Riau
No full textPenelitian ini bertujuan untuk meningkatkan minat siswa terhadap pembelajaran lari sprint dan hasil belajar keterampilan lari sprint 60 m melalui metode pendekatan permainan lari membawa gelang dan mangkok pada siswa kelas atas SD Negeri 009 Teluk Tahun Pelajaran 2013/2014 Kabupaten Pelalawan Riau.
Penelitian ini adalah penelitian tindakan kelas atau classroom action research yang berlangsung dua siklus, setiap siklus terdiri dari dua kali pertemuan. Subjek penelitian adalah siswa kelas V SD Negeri 009 Teluk Kabupaten Pelalawan Riau yang berjumlah 24 orang siswa. Adapun metode pengumpulan data yang digunakan adalah observasi, angket, tes unjuk kerja dan analisis data.
Hasil penelitian menunjukkan bahwa minat siswa terhadap pembelajaran lari sprint dan hasil belajar keterampilan lari sprint 60 m siswa mengalami peningkatan. Hasil pretest tentang minat siswa terhadap pembelajaran lari sprint 60 m sebesar 29% dan pada post-test meningkat sebesar 75% kategori berminat. Sedangkan hasil belajar keterampilan lari sprint 60 m siswa pada pretest dengan nilai rata-rata 60, pada siklus pertama pertemuan kedua meningkat dengan nilai rata-rata sebesar 64,58 dan siswa yang mencapai kriteria ketuntasan minimal 37% atau sebanyak 9 orang siswa. Siklus kedua, pertemuan kedua meningkat dengan nilai rata-rata sebesar 74,58 dan siswa yang mencapai kriteria ketuntasan minimal 100% atau 24 orang siswa.
Dengan demikian dapat disimpulkan bahwa permainan lari membawa gelang dan mangkok dapat meningkatkan hasil belajar keterampilan lari sprint 60 m pada siswa kelas atas SD Negeri 009 Teluk Kabupaten Pelalawan Ria
Urinary Untargeted Metabolic Profile Differentiates Children with Autism from Their Unaffected Siblings
No full textAutism Spectrum Disorder (ASD) encompasses a clinical spectrum of neurodevelopmental conditions that display significant heterogeneity in etiology, symptomatology, and severity. We previously compared 30 young children with idiopathic ASD and 30 unrelated typically-developing controls, detecting an imbalance in several compounds belonging mainly to the metabolism of purines, tryptophan and other amino acids, as well as compounds derived from the intestinal flora, and reduced levels of vitamins B6, B12 and folic acid. The present study describes significant urinary metabolomic differences within 14 pairs, including one child with idiopathic ASD and his/her typically-developing sibling, tightly matched by sex and age to minimize confounding factors, allowing a more reliable identification of the metabolic fingerprint related to ASD. By using a highly sensitive, accurate and unbiased approach, suitable for ensuring broad metabolite detection coverage on human urine, and by applying multivariate statistical analysis, we largely replicate our previous results, demonstrating a significant perturbation of the purine and tryptophan pathways, and further highlight abnormalities in the “phenylalanine, tyrosine and tryptophan” pathway, essentially involving increased phenylalanine and decreased tyrosine levels, as well as enhanced concentrations of bacterial degradation products, including phenylpyruvic acid, phenylacetic acid and 4-ethylphenyl-sulfate. The outcome of these within-family contrasts consolidates and extends our previous results obtained from unrelated individuals, adding further evidence that these metabolic imbalances may be linked to ASD rather than to environmental differences between cases and controls. It further underscores the excess of some gut microbiota-derived compounds in ASD, which could have diagnostic value in a network model differentiating the metabolome of autistic and unaffected siblings. Finally, it points toward the existence of a “metabolic autism spectrum” distributed as an endophenotype, with unaffected siblings possibly displaying a metabolic profile intermediate between their autistic siblings and unrelated typically-developing controls
Studies on the novel anti-staphyloccal compound nematophin
No full textA number of analogues of the recently described compound nematophin were prepared and studied for antibacterial activity. The 2-phenyl derivative was found to exhibit exceptional activity against methicillin resistant Staphylococcus aureus (MRSA) whereas the isosteric benzimidazole analogue was much less active
Multidisciplinary characterization of melanin pigments from the black fungus Cryomyces antarcticus
No full textMelanin is a natural pigment present in almost all biological group, and is composed by indolic polymers and characterized by black-brown colorization. Furthermore, it is one of the pigments produced by extremophiles including those living in Antarctic desert, and are mainly involved in their protection from high UV radiation, desiccation, salinity and oxidation. Previous studies have shown that melanized species have an increase capability to survive high level of radiation compared to the non-melanized counterpart. Understanding the molecular composition of fungal melanin could help to understand this peculiar capability. Here, we aimed to characterize melanin pigment extracted from the Antarctic black fungus Cryomyces antarcticus, which is a good test model for radioprotection researches, by studying its chemical properties and spectral data. Our results demonstrated that, in spite having a specific type of melanin as the majority of fungi, the fungus possess the ability to produce both DHN and DOPA melanins, opening interesting scenario for the protection role against radiation. Researches on fungal melanin have a huge application in different field, including radioprotection, bioremediation and biomedical applications
