9 research outputs found
Antimicrobial and anti-lipase activity of quercetin and its C2-C16 3-O-acyl-esters
No full textQuercetin (Q) and 3-O-acylquercetines, up to 100 microg/mL, did not show significant activity on selected gram-positive strains, gram-negative strains and yeasts. Conversely an interesting inhibitory activity on Candida rugosa lipase by Q and its esters (3-O-acylquercetines) which, in vivo, could play an important role against lipase producing microorganisms, was detected. In particular, 3-O-acyl-quercetines, being more active (IC(16)=10(-4)-10(-6) M) and more lipophilic, could be more effective than Q when applied to the skin or mucosae
Ibogaine reduces organ colonization in murine systemic and gastrointestinal Candida albicans infections
No full textIn the present study the effect of the indole alkaloid ibogaine on the in vitro
lipolytic activity and adherence to epithelial cells of Candida albicans was
investigated. The substance was administered intraperitoneally at a dose of 5 mg
kg(-1) day(-1) in mice with disseminated and gastrointestinal C. albicans
infections. Ibogaine significantly decreased the rate of mortality and the number
of C. albicans c.f.u. recovered from the kidney, liver and spleen. Ibogaine
interfered with the early stages of both disseminated and gastrointestinal C.
albicans infections but did not reduce the number of C. albicans c.f.u. in the
organs at the late phase of infections. The development of a specific immune
response was not influenced by ibogaine, since the delayed-type hypersensitivity
reaction to C. albicans and the production of interferon (IFN)-gamma were similar
in control and ibogaine-treated mice. The combined use of amphotericin B plus
ibogaine in the treatment of mice with gastrointestinal infection reduced organ
colonization more strongly than each substance alone
Inhibition of Candida rugosa lipase by saponins, flavonoids and alkaloids
No full textLipase inhibitors have generated a great interest because they could help in the prevention or the therapy of lipase-related diseases. Therefore, the aim of the work was to evaluate by HPLC, and using Candida rugosa lipase as model, the inhibitory effect of several saponins: beta-aescin, digitonin, glycyrrhizic acid (GA) and Quillaja saponin (QS); flavonoids: 3-hydroxyflavone, 5-hydroxyflavone, (+)-catechin and kaempferol; and alkaloids: aspidospermine, papaverine, physostigmine, pilocarpine, raubasine, rescinnamine, reserpine and trigonelline. The inhibition produced by most of these compounds is described here for the first time. Saponins appeared very active, being beta-aescin and digitonin the most active compounds (IC50 = 0.8-2.4 x 10(-5) M). The inhibitory activity of flavonoids was lower than that of saponins (except GA), and (+)-catechin and kaempferol were the most active. Alkaloids was the most heterogeneous group assayed, varying from rescinnamine, with an IC16 similar to that of digitonin, to papaverine and others which showed almost no inhibition. In conclusion, beta-aescin, digitonin, kaempferol or (+)-catechin, strong lipase inhibitors with a low toxicity and present herbal drugs used for lipase-related diseases such as acne or ulcer, are promising candidates for the prevention or the treatment of these diseases. (c) 2006 Elsevier B.V. All rights reserved
TGF-BETA INTERFERES WITH INSULIN-LIKE GROWTH FACTOR SIGNALING PATHWAY IN FRTL-5 THYROID EPITHELIAL CELL LINE.
No full textSURPRISE PARTY N °15
No full textComprend : LE GRISBI : du film "Touchez pas au grisbi" / JEAN WIENER ; TONY MURENA et son Quintette - LE PETIT CORDONNIER : Fox trot / Adaptation de REVIL ; MARC TAYNOR et son Orchestre de danse - LA PETITE MULE : Boléro / FALCOCCHIO - JUDAS (Come Giuda) Boléro / FANCIULLU ; RAMON MENDIZABAL et son Orchestre - COCO COCONUT (Noix de coco) Mambo / P. ALENCAR - MON P'TIT FICHU : Baiao / HERVE CORDOVIL ; EDDIE WARNER et sa musique tropicale - DU SOLEIL PLEIN LA TETE : Fox trot / HENRI CROLLA ; JACQUES PASTORY et son Orchestre - LA GOUALANTE DU PAUVRE JEAN : Fox style 1925. / M. MONNOT ; EMILE PRUD'HOMME et son EnsembleBnF-Partenariats, Collection sonore - BelieveContient une table des matière
Surprise partie au Champ de Mars
No full textTitre uniforme : [Ay, mi sombrero !]Titre uniforme : [La Vague]Titre uniforme : [Caminito]Comprend : Le premier rendez-vous : fox-trot du film du même titre / René SYLVIANO ; Barbara MONROE et orchestre - Berger d'autrefois : fox-trot du film "La fausse maîtresse" / Maurice YVAIN ; Barbara MONROE et orchestre - La petite mule / ZOCCOLETTI boléro Eduardo FALCOCCHIO - La danse à la mode ("A dança da moda") baïao / Luiz GONZAGA ; Roger DAVID et son orchestre - La valse de juillet / M. PHILIPPE-GERARD arr. R. BROGIOTTI - La vague : valse / Olivier METRA ; BROGIOTTI et orch. - CAMINITO - tango / FILIBERTO - DONDE ESTAS CORAZON - tango / A. BERTO ; Manuel PIZARRO et son orchestre - Bonne chance ("Mazeltov") boléro / José del MONTERO - AY, MI SOMBRERO - paso-doble / MONREAL - Rose-Marie polka / Michaël HARDEN - Fanfan-la-tulipe : fox / Ludo LANGLOIS ; Jack VIGOUROUX et son orchestre - Le paradis - marchina / Daniel MONTORIO - Je-te-le-le : valse / José CANA ; Roger DAVID et son orchestre - Que reste-t-il de nos amours : slow-fox / Charles TRENET - MENILMONTANT - fox trot / Charles TRENET ; Barbara MONROE et orchestreBnF-Partenariats, Collection sonore - BelieveContient une table des matière
LE PETIT TRAIN / Marc FONTENOY. LA PETITE MULE / BONIFAY et FALCOCCHIO. UN HOMME EST UN HOMME / Jacques LARUE et O. BRAND. OH ! BOY / M. VANDAIR et A. FRAGNA ; Jacques HELIAN et son orchestre
No full textBnF-Partenariats, Collection sonore - BelieveContient une table des matière
Integrating best practice and filling knowledge gaps in remote Aboriginal diabetes detection and care: Improving case detection and service delivery
No full textThis pilot project will provide detailed feedback on the results of previous studies to participating services. During discussions with clinic and management staff, barriers and enablers to implementing a new screening protocol using HbA1c testing will be determined. The project will also conduct a clinical systems and software / data quality needs assessment at participating sites, documenting strategies to improve diabetes programs and monitor short term changes resulting from the audit.The research reported in this paper is a project of the Australian Primary Health Care Research Institute which is supported by a grant from the Australian Government Department of Health and Ageing under the Primary Health Care Research Evaluation and Development Strategy
Produção e caracterização parcial de lipases com atividade de hidrólise e de síntese por fermentação em estado sólido de farelo de soja
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2009Este trabalho buscou isolar e selecionar novos microrganismos capazes de produzir lipases com atividade de hidrólise e de esterificação, e avaliar a produção e a caracterização de lipases extracelulares por fermentação em estado sólido (FES) em farelo de soja com os microrganismos selecionados. Observouse que dentre os microrganismos isolados, os fungos filamentosos do gênero Penicillium apresentaram os melhores resultados como produtores de lipase com atividade hidrolítica. Por outro lado as cepas de leveduras foram as que apresentaram melhor desempenho na catálise da reação de produção de n-propil oleato, sendo somente duas cepas fúngicas selecionadas entre os 17 microrganismos hábeis para produção de lipase com atividade de esterificação. A fermentação em estado sólido para produção de lipase com atividade hidrolítica usando farelo de soja apresentou resultados promissores. O estudo da suplementação revelou o Penicillium 58F como o mais eficiente para produção de lipase hidrolítica, nos distintos valores de pH avaliados, sendo a suplementação com uréia acrescida de óleo de soja a que resultou nas melhores atividades. A técnica de planejamento de experimentos mostrou-se eficiente para maximização do processo fermentativo para produção de lipase hidrolítica. O processo foi maximizado com suplementação de 0,60% de uréia acrescida de óleo de soja para atingir relação de C/N 6,11, umidade 75%, granulometria farelo de soja de 1-2 mm, concentração de inóculo de 2x108 esporos/g e 20 °C. A atividade hidrolítica máxima obtida em meio ácido foi de 200 U/g farelo de soja seco após 120h de fermentação, em pH neutro de 317 U/g e em alcalino obteve-se atividade de 191 U/g farelo de soja seco, ambos em 96 h fermentação. Os resultados demonstram haver produção de altas concentrações de lipase extracelular, possivelmente um pool de lipases, com potencial hidrolítico em uma ampla faixa de pH. A relação C/N de 6,11 foi a que resultou nas melhores produtividades. A condição otimizada para extração da lipase foi usando tampão fosfato de sódio 100mM pH 7,0 e temperatura de 25ºC, 150 rpm, e razão sólido/líquido de 1:4, com incubação de 15 minutos. A caracterização parcial do extrato enzimático hidrolítico bruto e pré-purificado apresentou temperatura ótima de atividade de 37ºC, para ambas, e pH ótimo igual a 7 e na faixa de 9 a 10, respectivamente. A maior estabilidade dos extratos bruto e prépurificados foi a 25 ºC e pH 7. A exposição a CaCl2 levou à inativação do extrato enzimático hidrolítico pré-purificado. A especificidade do extrato enzimático hidrolítico pré-purificado indicou atividades em ésteres com uma faixa de comprimento de cadeia variando de C4 a C18.This work aimed to isolating and selecting new lipase-producing microorganisms, able to produce lipase both with synthesis and hydrolysis activities, and to assess the production and characterization of extracellular lipases, by solid state fermentation (SSF) using soybean meal as substrate, with the microorganisms isolated in the previous step. The filamentous fungi of the genera Penicillium presented the best production results in terms of hydrolytic activity. The best performance on production of lipases with synthesis activity (n-propyl oleate) was obtained with the yeast strains, while only two fungal strains produced lipases with a good potential for synthesis among the 17 selected strains. The use of soybean meal as substrate for SSF presented promising results. The study on the supplementation of this substrate showed that the Penicillium sp. 58F was the most potential lipase producer, considering three different pH values. The use of urea and soybean oil resulted in the highest lipase activities. The lipase production could be optimized using the experimental design technique. Maximum activity was obtained using soybean meal (75% moisture and particle size 1-2 mm) supplemented with 0.6% urea and soybean oil to reach a C/N ratio of 6.11, 2x108 spores/g and 20 °C. The resulting activity in acid medium was 200 U/g of dry substrate after 120 h of fermentation. In neutral and alkaline medium maximum activities were 317 U/g and 191 U/g after 96 h of fermentation, respectively. The results suggest that a pool of lipases is produced, with a high lipolytic activity in a wide pH range. The C/N ratio 6.11 maximized the lipase production. The optimum extraction condition was phosphate buffer (pH 7.0 100 mM), 25ºC, 150 rpm, solid to liquid ratio 1:4 and incubation for 15 min. Partial characterization of the crude and pre-purified extract showed that both presented optimum temperature for activity of 37ºC and optimum pH of 7 and 9-10, respectively. The crude and pre-purified extract were more stable at 25ºC and pH 7.0. The exposure of the extract to CaCl2 induced to a inactivation of the pre-purified extract. The specificity of the pre-purified extract showed that good activities could be obtained in long chain p-nitro esters and triacylglycerols
