250 research outputs found
Development of a Sandwich ELISA to Measure Exposure to Occupational Cow Hair Allergens
No full textBackground: Cow hair and dander are important inducers of occupational allergies in cattle-exposed farmers. To estimate allergen exposure in farming environments, a sensitive enzyme immunoassay was developed to measure cow hair allergens. Methods: A sandwich ELISA was developed using polyclonal rabbit antibodies against a mixture of hair extracts from different cattle breeds. To assess the specificity of the assay, extracts from other mammalian epithelia, mites, molds and grains were tested. To validate the new assay, cow hair allergens were measured in passive airborne dust samples from the stables and homes of farmers. Dust was collected with electrostatic dust fall collectors (EDCs). Results: The sandwich ELISA was found to be very sensitive (detection limit: 0.1 ng/ml) and highly reproducible, demonstrating intra-and interassay coefficients of variation of 4 and 10%, respectively. The assay showed no reactivity with mites, molds and grains, but some cross-reactivity with other mammalian epithelia, with the strongest reaction with goat. Using EDCs for dust sampling, high concentrations of bovine allergens were measured in cow stables (4,760-559,400 mu g/m(2)). In addition, bovine allergens were detected in all areas of cattle farmer dwellings. A large variation was found between individual samples (0.3-900 mu g/m(2)) and significantly higher values were discovered in changing rooms. Conclusion: The ELISA developed for the detection of cow hair proteins is a useful tool for allergen quantification in occupational and home environments. Based on its low detection limit, this test is sensitive enough to detect allergens in passive airborne dust. Copyright (C) 2011 S. Karger AG, Base
Contact urticaria to giraffe hair
No full textBackground: Immediate-type hypersensitivity to animal proteins is a common problem in people occupationally exposed to animals. Methods: A 19-year-old female working as a voluntary zookeeper in her off-time suffered from hives on her forearms following contact to the fur of a giraffe. For diagnostic evaluation, skin prick tests, assessment of specific serum IgE antibodies, and basophil activation tests were performed. Results: Skin prick tests with a standard series of common aeroallergens were positive for various pollens. Prick testing with native materials was positive for extracts of hair from two different giraffe subspecies in the patient, but not in control subjects. By CAP-FEIA, no specific serum IgE antibodies to dander of a large variety of animals were found in the patient. In the basophil activation test, expression of the activation marker CD63 was induced by extract of giraffe hair on the cells from the patient, but not on those from unaffected controls. Conclusions: This patient suffers from an `exotic' immediate-type contact allergy to giraffe hair. Copyright (C) 2005 S. Karger AG, Basel
Ricerca, documentazione, salvaguardia, valorizzazione e gestione nell'ambito del costituendo parco paleolitico di Isernia La Pineta (Molise, Italia)
No full textGeneration YouTube: The Demolition of Politics or the Continuation of Politics with Different Means
No full textValentin Dander analysiert in seinem Beitrag die aktuelle Diskussionskultur im soziodigitalen Raum anhand der Rezeption der Videos des 26-jährigen YouTubers Rezo und konstatiert dabei eine Verschiebung in der Wahrnehmung des Politischen. Ausgehend von Rezos YouTube-Video „Die Zerstörung der CDU“ geht Dander der Frage nach jugendlichen Praktiken der politischen Informationsbeschaffung und Meinungsbildung nach. Er begreift daher YouTube als einen Ort politischer Information und Bildung Jugendlicher, die jenseits der Schriftkultur vor allem das Visuelle bedient. Hierbei wird insbesondere danach gefragt, welche Rolle Online-Videos für das politische Informationsverhalten Jugendlicher spielen, welche Differenz sich hierbei zu älteren Generationen öffnet sowie danach, inwieweit von einem generellen Wandel der politischen Sphäre gesprochen werden kann. Der Autor betont deshalb, dass Politik und Medienkultur in der Wissens- und Informationsgesellschaft des 21. Jahrhunderts in und jenseits von Generationenverhältnissen zu denken ist. Deshalb steht uns als Prosumerinnen und Prosumern eine neuartige Form der politischen Digitalisierung und der digitalen Politik vor Augen.In his contribution, Valentin Dander analyzes the current culture of discussion in the socio-digital space through the reception of videos by the 26-year-old YouTube creator Rezo, stating a shift in the perception of the political. He therefore understands YouTube as a place of political information and education for young people who, beyond the written culture, primarily serve the visual. The author therefore emphasizes that politics and media culture can be considered in the knowledge and information society of the 21st century in and beyond generational relationships. Therefore, as a prosumer, we see a new kind of political digitization and digital politics in mind
Allergy to human seminal fluid: Cross-reactivity with dog dander
No full textBackground: Human seminal plasma (HSP) allergy is uncommon, with symptoms ranging from vulvovaginal pruritus to life-threatening anaphylaxis. Although several seminal plasma allergens have been reported and their molecular masses have been estimated to range between 12 and 75 kd, the prostate-specific antigen (PSA) has recently been identified as a causative allergen. Given that in a large number of cases symptoms appeared during or after the first intercourse, a cross-reactivity phenomenon might be implicated.
Objective: We sought to assess the presence of IgE cross-reactivity among proteins from dog epithelium and HSP and to attempt to identify the allergens involved.
Methods: Forty-one patients with dog epithelium allergy were selected. One of them experienced anaphylaxis in contact with her husband's seminal plasma. Skin prick tests, serum specific IgE measurements, SDS-PAGE immunoblotting, and inhibition tests were performed to study the pattern of IgE-binding proteins and the potential cross-reactivity between HSP and dog epithelium. Mass spectrometry was carried out to identify the protein involved in allergy reactions.
Results: Twenty-four percent of the sera from patients with dog epithelium allergy recognized an IgE-binding band of 28 kd in HSP immunoblotting. Mass spectrometry identified this band as the PSA. SDS-PAGE immunoblotting-inhibition showed a complete IgE-binding inhibition when sera from these patients were preincubated with dog dander extract.
Conclusions: IgE cross-reactivity among proteins from dog dander and human PSA is demonstrated.Depto. de Bioquímica y Biología MolecularFac. de Ciencias QuímicasTRUEpu
Inklusion im Kontext medienpädagogischer Forschung und Lehre. Perspektiven der Medienpädagogik zum Diskussionspapier „Inklusion als unverzichtbarer Bestandteil erziehungswissenschaftlicher Lehre“ der AG Inklusionsforschung
No full textKamin A-M, Dander V. Inklusion im Kontext medienpädagogischer Forschung und Lehre. Perspektiven der Medienpädagogik zum Diskussionspapier „Inklusion als unverzichtbarer Bestandteil erziehungswissenschaftlicher Lehre“ der AG Inklusionsforschung. Erziehungswissenschaft. 2024;Heft 69, 35(2-2024):95-102
F. Dander, Summarium tractatus dogmatici De Fide Divina, 1950
No full textN. M. F. Dander, Summarium tractatus dogmatici De Fide Divina, 1950. In: Revue des Sciences Religieuses, tome 24, fascicule 3-4, 1950. p. 415
F. Dander, Summarium tractatus dogmatici De Fide Divina, 1950
No full textN. M. F. Dander, Summarium tractatus dogmatici De Fide Divina, 1950. In: Revue des Sciences Religieuses, tome 24, fascicule 3-4, 1950. p. 415
Sejarah perkembangan Pondok Pesantren Al Rosyid Desa Ngumpakdalem Kecamatan Dander Kabupaten Bojonegoro tahun 1959-2016 m
No full textSkripsi ini adalah hasil penelitian lapangan yang berjudul “Sejarah Perkembangan Pondok Pesantren Al Rosyid Desa Ngumpakdalem Kecamatan Dander Kabupaten Bojonegoro”. Adapun rumusan masalah sebagai berikut: 1. Bagaimana sejarah berdirinya Pondok Pesantren Al Rosyid Desa Ngumpakdalem Kecamatan Dander Kabupaten Bojonegoro dari tahun 1959-2016? 2. Bagaimana perkembangan Pondok Pesantren Al Rosyid Desa Ngumpakdalem Kecamatan Dander Kabupaten Bojonegoro dari tahun 1959-2016? 3. Bagaimana kontribusi Pondok Pesantren Al Rosyid Desa Ngumpakdalem Kecamatan Dander Kabupaten Bojonegoro terhadap perkembangan masyarakat sekitar? Penelitian ini menggunakan metode penelitian sejarah yang mencakup heuristik, kritik sumber, interpretasi, dan historiografi. Penulis menggunakan pendekatan sosiologi yang mencakup teori fungsionalisme struktural dan teori kepemimpinan karismatik. Hasil penelitian ini dapat disimpulkan bahwa: 1. Pondok Pesantren Al Rosyid didirikan oleh KH. Masyhur pada tahun 1959, yang bertujuan untuk memperbaiki dan membangun mental masyarakat sesuai dengan ajaran-ajaran islam dan dapat menanamkan rasa keagamaan pada sumua segi kehidupan dalam masyarakat. 2. Perkembangannya pun begitu pesat yaitu karena banyaknya santri yang berdatangan untuk mondok dan bersekolah di Pondok Pesantren Al Rosyid, sehingga mengalami berbagai perkembangan perubahan sistem pendidikan, namun tidak meninggalkan pendidikan klasiknya. Seperti meningkatkan model sistem pendidikan yang memadukan antara pendidikan klasik dengan sistem pendidikan modern. 3. Adapun pengaruh Pondok Pesantren Al Rosyid terhadap masyarakat yang menghasilkan dampak positif, seperti halnya keikutsertaan masyarakat dalam program kegiatan yang dilakukan Pondok Pesantren Al Rosyid untuk menanamkan rasa keagamaan pada masyarakat itu sendiri
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