408 research outputs found
Replication Data for: Constraint-aware neural networks for Riemann problems
No full textData sets of the article "Constraint-aware neural networks for Riemann problems", consisting of training and test data sets for Riemann solutions of the cubic flux model, an isothermal two-phase model, and the Euler equations for an ideal gas. You can find detailed information in the README.md
Maturity Model for the Digital Transformation of the Road and Bridge Construction Sectors in Poland. Prospective directions for the development of subsequent BIM-M
No full textW artykule omówiona jest całościowa wizja rozwoju standardów dotyczących cyfryzacji branży drogowej i mostowej, uwzględniająca perspektywy całego cyklu życia infrastruktury drogowej i mostowej, jak i ich różnych interesariuszy (projektantów, wykonawców, zarządców, operatorów oraz organa administracji publicznej, w szczególności rozwój systemów e-administracji/Inteligentnej Infrastruktury/Cyfrowego Bliźniaka Kraju). Materiał przedstawiany w niniejszej publikacji jest wynikiem pracy autora w Komitecie Technicznym Cyfryzacji (KTC) przy Ministrze Infrastruktury (MI) oraz Polskim Kongresie Drogowym (PKD) i jest propozycją nadania pewnej struktury i porządku dla opracowywania kolejnych – po BIM M-01 i BIM D-01 – opracowań z serii Wzorców i Standardów. Są one zaproponowane w odniesieniu i spójnie z opracowanym niezależnie Modelem Dojrzałości Transformacji Cyfrowej Polskiego Budownictwa, które zostało opracowane przez autora na rzecz Grupy Roboczej ds. BIM przy Ministrze Rozwoju i Technologii (GRdsBIM, MRiT).In this paper discussed is a broad vision of the development of standards for the digitization of the road and bridge infrastructure, taking into account the perspectives of their entire life cycle as well as their various stakeholders (designers, contractors, managers, operators and public administration bodies, in particular the development of systems for e-administration/Intelligent Infrastructure/National Digital Twin). The material presented in this publication resulted from the author’s work in the Technical Committee for Digitization (KTC), Ministry of Infrastructure (MI) and the Polish Road Congress (PKD). Its main objective is to propose a hierarchical structure and order for the development of subsequent - after the BIM M-01 and BIM D 01 – standards in the Wzorce i Standardy series of publications. They are proposed in relation to and consistently with the independently developed Digital Transformation Maturity Model for the Polish Construction Industry, which was developed by the author for the BIM Task Group at the Ministry of Development and Technology (GRdsBIM, MRiT)
DNA replication and spindle checkpoints cooperate during S phase to delay mitosis and preserve genome integrity
No full textDeoxyribonucleic acid (DNA) replication and chromosome segregation must occur in ordered sequence to maintain genome integrity during cell proliferation. Checkpoint mechanisms delay mitosis when DNA is damaged or upon replication stress, but little is known on the coupling of S and M phases in unperturbed conditions. To address this issue, we postponed replication onset in budding yeast so that DNA synthesis is still underway when cells should enter mitosis. This delayed mitotic entry and progression by transient activation of the S phase, G2/M, and spindle assembly checkpoints. Disabling both Mec1/ATR- and Mad2-dependent controls caused lethality in cells with deferred S phase, accompanied by Rad52 foci and chromosome missegregation. Thus, in contrast to acute replication stress that triggers a sustained Mec1/ATR response, multiple pathways cooperate to restrain mitosis transiently when replication forks progress unhindered. We suggest that these surveillance mechanisms arose when both S and M phases were coincidently set into motion by a unique ancestral cyclin-Cdk1 complex
Solubilizer Tag Effect on PD-L1/Inhibitor Binding Properties for m-Terphenyl Derivatives
No full textAlthough heavily studied, the subject of anti-PD-L1 small-molecule inhibitors is still elusive. Here we present a systematic overview of the principles behind successful anti-PD-L1 small-molecule inhibitor design on the example of the m-terphenyl scaffold, with a particular focus on the neglected influence of the solubilizer tag on the overall affinity toward PD-L1. The inhibitor developed according to the proposed guidelines was characterized through its potency in blocking PD-1/PD-L1 complex formation in homogeneous time-resolved fluorescence and cell-based assays. The affinity is also explained based on the crystal structure of the inhibitor itself and its costructure with PD-L1 as well as a molecular modeling study. Our results structuralize the knowledge related to the strong pharmacophore feature of the m-terphenyl scaffold preferential geometry and the more complex role of the solubilizer tag in PD-L1 homodimer stabilization
Can Satellite Remote Sensing be Applied in Geological Mapping in Tropics?
No full textRemote sensing (RS) techniques are based on spectral data registered by RS scanners as energy reflected from the Earth’s surface or emitted by it. In “geological” RS the reflectance (or emittence) should come from rock or sediment. The problem in tropical and subtropical areas is a dense vegetation. Spectral response from the rocks and sediments is gathered only from the gaps among the trees and shrubs. Images of high resolution are appreciated here, therefore. New generation of satellites and scanners (Digital Globe WV2, WV3 and WV4) yield imagery of spatial resolution of 2 m and up to 16 spectral bands (WV3). Images acquired by Landsat (TM, ETM+, OLI) and Sentinel 2 have good spectral resolution too (6–12 bands in visible and infrared) and, despite lower spatial resolution (10–60 m of pixel size) are useful in extracting lithological information too. Lithological RS map may reveal good precision (down to a single rock or outcrop of a meter size). Supplemented with the analysis of Digital Elevation Model and high resolution ortophotomaps (Google Maps, Bing etc.) allows for quick and cheap mapping of unsurveyed areas
Trade Update Notes; Report of the Task Force on The Aggregate Measure of Support: Potential Use by GATT for Agriculture
No full textInternational Relations/Trade,
Trim17, novel E3 ubiquitin-ligase, initiates neuronal apoptosis
No full textAccumulating data indicate that the ubiquitin-proteasome system controls apoptosis by regulating the level and the function of key regulatory proteins. In this study, we identified Trim17, a member of the TRIM/RBCC protein family, as one of the critical E3 ubiquitin ligases involved in the control of neuronal apoptosis upstream of mitochondria. We show that expression of Trim17 is increased both at the mRNA and protein level in several in vitro models of transcription-dependent neuronal apoptosis. Expression of Trim17 is controlled by the PI3K/Akt/GSK3 pathway in cerebellar granule neurons (CGN). Moreover, the Trim17 protein is expressed in vivo, in apoptotic neurons that naturally die during post-natal cerebellar development. Overexpression of active Trim17 in primary CGN was sufficient to induce the intrinsic pathway of apoptosis in survival conditions. This pro-apoptotic effect was abolished in Bax(-/-) neurons and depended on the E3 activity of Trim17 conferred by its RING domain. Furthermore, knock-down of endogenous Trim17 and overexpression of dominant-negative mutants of Trim17 blocked trophic factor withdrawal-induced apoptosis both in CGN and in sympathetic neurons. Collectively, our data are the first to assign a cellular function to Trim17 by showing that its E3 activity is both necessary and sufficient for the initiation of neuronal apoptosis. Cell Death and Differentiation (2010) 17, 1928-1941; doi: 10.1038/cdd.2010.73; published online 18 June 201
A multi-level method of support for management of product flow through supply chains
No full textThe paper presents a three-level method used to support the management of product flow through supply chain links (e.g. production lines) and between these links: suppliers and recipients of products of various types.The supply chain includes both producers of components (suppliers) and recipients of these components, which are used for the production of complex goods. The method is dedicated to the development of schedules of product flows through particular production plants (links in supply chains) and between individual plants. Each module of the developed system refers to a separate production plant. The organization of product flow through production lines covers different types of production routes and different configurations of production systems. At the first level of the method, preliminary production schedules are developed for each plant within the supply chain. The second level of the method is dedicated to the development of delivery schedules of components and semi-finished products to these plants. The determined delivery times of components to the individual production plants constitute data for the third level of the method. At this lowest level, detailed schedules of product flow through production lines with the producers of complex goods are developed. Linear mathematical models have been built for each level of the method. In the developed method, optimization take place in the developed method in the scale of the entire supply chain (cost reduction), as well as in the scale of its links (production lines for which manufacturing schedules are built with various criteria taken into consideration). The computational experiments used for verification of the method have been included
Methods of planning deliveries of food products to a trade network with the selection of suppliers and transport companies
No full textThe paper refers to planning deliveries of food products (especially those available in certain
seasons) to the recipients: supermarket networks. The paper presents two approaches to
solving problems of simultaneous selection of suppliers and transportation modes and construction
of product flow schedules with these transportation modes. Linear mathematical models
have been built for the presented solution approaches. The cost criterion has been taken into
consideration in them. The following costs have been taken into account: purchase of products
by individual recipients, transport services, storing of products supplied before the planned
deadlines and penalties for delays in supply of products. Two solution approaches (used for
transportation planning and selection of suppliers and selection of transportation modes) have
been compared. The monolithic approach calls for simultaneous solutions for the problems of
supplier selection and selection of transportation modes. In the alternative (hierarchical) solution
approach, suppliers are selected first, and then transportation companies and their relevant
transportation modes are selected. The results of computational experiments are used for comparison
of the hierarchical and monolithic solution approaches
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