41 research outputs found
Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture
No full textHakki Oguz Kazancioglu,1 Sertac Aksakalli,2 Seref Ezirganli,1 Muhammet Birlik,2 Mukaddes Esrefoglu,3 Ahmet Hüseyin Acar1 1Department of Oral and Maxillofacial Surgery, 2Department of Orthodontics, Faculty of Dentistry, 3Department of Histology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey Background: Narrow maxilla is a common problem in orthodontics and dentofacial orthopedics. To solve this problem, a procedure called rapid maxillary expansion (RME) has been used. However, relapse tendency is a major problem of RME. Although relapse tendency is not clearly understood, various treatment procedures and new application has been investigated. The present study aimed to investigate the possible effectiveness of caffeic acid phenethyl ester (CAPE) on new bone formation in rat midpalatal suture after RME.Materials and methods: Twenty male Sprague Dawley rats were used in this study. The animals were randomly divided into two groups as control and CAPE group. In CAPE group, CAPE was administered systemically via intraperitoneal injection. RME procedure was performed on all animals. For this purpose, the springs were placed on the maxillary incisors of rats and activated for 5 days. After then, the springs were removed and replaced with short lengths of rectangular retaining wire for consolidation period of 15 days. At the end of the study, histomorphometric analysis was carried out to assess of new bone formation.Results: New bone formation was significantly greater in CAPE group than the control group (P<0.05). CAPE enhances new bone formation in midpalatal suture after RME.Conclusion: These results show that CAPE may decrease the time needed for retention. Keywords: rapid maxillary expansion, bone formation, caffeic acid phenethyl ester, midpalatal suture, histopatholog
Ultrastructural clues for glutamate-induced necrosis in parietal and cerebellar neurons
No full textGlutamate excitotoxicity has been postulated to underlie the neuronal death that occurs after ischemia. The most sensitive tissues to ischemic injury are hippocampus and cerebellum, whereas cerebrum is more resistant. We studied the glutamate-induced ultrastructural alterations in rat parietal and cerebellar neurons comparatively. We observed that glutamate (45 min, 10(-7) M) causes considerable nuclear, mitochondrial and cytoplasmic changes in both the neuron types. Mitochondrial and nuclear changes were particularly more severe in cerebellar granular, than the ones in parietal neurons. It has been concluded that glutamate induces necrotic changes in both parietal and cerebellar neurons. But cerebellar cortex was found to be more sensitive to glutamate excitotoxicity than cerebral cortex. We suggest that mitochondrial damage is, probably, an important factor in neuron necrosis, which is mediated by glutamate excitotoxicity
Modeling of mosques with fotogrametrik method
No full texturl: http://sutod.selcuk.edu.tr/sutod/article/view/297Kültürel miraslar atalarımız tarafından bizlere bırakılmış en değerli hazinelerdir. Bu nadide eserlerin gelecek nesillere taşınması, kıymetini bilen her bilinçli kişinin görevidir. Ancak bu kültür hazineleri doğal ve doğal olmayan birçok etkilerden zarar gördüğü için, gelecek nesillere aktarılmasında belgeleme çalışmalarının yapılması gerekmektedir. Belgeleme çalışmaları hem eserin zarar gördüğünde yeniden yapımı için bir plan altlığını oluşturur hem de eserde meydana gelen değişikliklerin incelenmesine, analiz edilmesine, durumunun takip edilmesine imkan tanır. Günümüzde genellikle mimarlar tarafından yapılan bu belgeleme çalışmalarında artık çağdaş teknolojilerin kullanılması bir zorunluluk olmuştur. Klasik mimari belgelemede yani röleve çalışmalarında ve restorasyon projelerinin hazırlanmasında çoğu zaman basit ölçme araç ve teknikleri kullanılmakta ve sonuç ürün olarak da tamamen kağıt baskılar olarak eserlerin çizimleri sunulmaktadır. Bu çalışmada, Eşrefoğlu Camii araştırılmıştır. Konya’nın Beyşehir İlçesinde, Beyşehir Gölü’nün 100m. kuzeyinde ağaç direkleriyle, ahşap tavanı, minberi ve yüzyılların sükûneti ile görkemli bir ahşap camii olan Eşrefoğlu Camii; 31,80x46,55m ebadındadır. Beyşehir’in önemli tarihi eserlerinden olan Eşrefoğlu Camii, Konya’ya 72km mesafededir, Bu çalışma ile Eşrefoğlu Cami’nin fotogrametrik olarak ölçülmesi, 3B modellenmesi ve gerçek dokuları ile kaplanması amaçlanmıştır. Ayrıca caminin çatısının modellenmesi için insansız hava araçları da kullanılmış, çekilen fotoğraflar da değerlendirilmiştir.Cultural heritages are the most valuable treasure left to us by our ancestries. Moving of this precious work of arts to next generations is a dream of every concious person who know worths of this treasures. However, moving this treasure to the next generation, documentation works are needed for this cultural treasures due to damaging with natural and un-natural effects. Documentation process both its constitudes as a base plan when damaged for rebuilding and it is enable to analysis, monitoring, pursue of status, investigations of changes on work of art. Nowadays, modern technologies should be used in documentary works which made by architects. Many times, in classical architectural documentation that is roleve works and in the restoration project simple survey instruments and technics are used and drawings of work of arts on paper presented as a final production. This study investigated the Esrefoglu mosque. Esrefoglu mosque, which is a magnificent wooden mosque with wooden columns, timber ceiling and mimbar, and tranquility of the centuries, has dimensions of 31,80x46,55m in Beyşehir County of Konya, 100 m away from the Beyşehir Lake. Esrefoglu Mosque as one of the important historical artifacts of Beysehir is at a distance of 72 km to Konya. In this study, it is aimed to photogrammetric measurement, 3Dmodelingandcover with the real layers of Esrefoglu mosgue. In addition, unmanned aerial vehicles were being used for the modeling of the mosque's roof and the photos were evaluated
YERSEL FOTOGRAMETRIK YÖNTEM İLE İBADETHANELERİN MODELLENMESİ
No full textYERSEL FOTOGRAMETRIK YÖNTEM İLE İBADETHANELERİN MODELLENMESİÖzet Kültürel miraslar atalarımız tarafından bizlere bırakılmış en değerli hazinelerdir. Bu nadide eserlerin gelecek nesillere taşınması, kıymetini bilen her bilinçli kişinin görevidir. Ancak bu kültür hazineleri doğal ve doğal olmayan birçok etkilerden zarar gördüğü için, gelecek nesillere aktarılmasında belgeleme çalışmalarının yapılması gerekmektedir. Belgeleme çalışmaları hem eserin zarar gördüğünde yeniden yapımı için bir plan altlığını oluşturur hem de eserde meydana gelen değişikliklerin incelenmesine, analiz edilmesine, durumunun takip edilmesine imkan tanır. Günümüzde genellikle mimarlar tarafından yapılan bu belgeleme çalışmalarında artık çağdaş teknolojilerin kullanılması bir zorunluluk olmuştur. Klasik mimari belgelemede yani röleve çalışmalarında ve restorasyon projelerinin hazırlanmasında çoğu zaman basit ölçme araç ve teknikleri kullanılmakta ve sonuç ürün olarak da tamamen kağıt baskılar olarak eserlerin çizimleri sunulmaktadır. Bu çalışmada, Eşrefoğlu Camii araştırılmıştır. Konya’nın Beyşehir İlçesinde, Beyşehir Gölü’nün 100m. kuzeyinde ağaç direkleriyle, ahşap tavanı, minberi ve yüzyılların sükûneti ile görkemli bir ahşap camii olan Eşrefoğlu Camii; 31,80x46,55m ebadındadır. Beyşehir’in önemli tarihi eserlerinden olan Eşrefoğlu Camii, Konya’ya 72km mesafededir, Bu çalışma ile Eşrefoğlu Cami’nin fotogrametrik olarak ölçülmesi, 3B modellenmesi ve gerçek dokuları ile kaplanması amaçlanmıştır. Ayrıca caminin çatısının modellenmesi için insansız hava araçları da kullanılmış, çekilen fotoğraflar da değerlendirilmiştir.Anahtar Kelimeler: Kültürel Miras, 3B model, Yersel Fotogrametri.MODELİNG OF MOSQUES WITH FOTOGRAMETRİK METHODAbstractCultural heritages are the most valuable treasure left to us by our ancestries. Moving of this precious work of arts to next generations is a dream of every concious person who know worths of this treasures. However, moving this treasure to the next generation, documentation works are needed for this cultural treasures due to damaging with natural and un-natural effects. Documentation process both its constitudes as a base plan when damaged for rebuilding and it is enable to analysis, monitoring, pursue of status, investigations of changes on work of art. Nowadays, modern technologies should be used in documentary works which made by architects. Many times, in classical architectural documentation that is roleve works and in the restoration project simple survey instruments and technics are used and drawings of work of arts on paper presented as a final production. This study investigated the Esrefoglu mosque. Esrefoglu mosque, which is a magnificent wooden mosque with wooden columns, timber ceiling and mimbar, and tranquility of the centuries, has dimensions of 31,80x46,55m in Beyşehir County of Konya, 100 m away from the Beyşehir Lake. Esrefoglu Mosque as one of the important historical artifacts of Beysehir is at a distance of 72 km to Konya. In this study, it is aimed to photogrammetric measurement, 3Dmodelingandcover with the real layers of Esrefoglu mosgue. In addition, unmanned aerial vehicles were being used for the modeling of the mosque's roof and the photos were evaluated.Keywords: Cultural Heritages, 3D Model, Terrestrial Photogrammetry
Nimodipine improves kainic acid induced neurotoxicity in cerebellar granular cell culture: A double-blind dose-response study
No full textThe neuroprotective role of nimodipine was tested in kainic acid (50 and 100 mu M) induced neurotoxicity in cerebellar granular cell cultures of 4 to 7 day-old rat pups. Nimodipine was applied in 50, 100 and 200 mu M concentrations. Kainate, in either dose, induced cerebellar granular cell death in respect to controls and the results were statistically significant (P = 0.000 for both doses). However, kainic acid in 100 mu M concentration led to higher rates of cell death than 50 mu M (P = 0.017). The neuroprotective role of nimodipine in kainate induced neurotoxicity was dose dependent. Kainate toxicity in 50 mu M concentration was blocked by 50 and 100 mu M nimodipine concentrations (P = 0.006 and P = 0.002, respectively) while 200 mu M nimodipine was found ineffective. The most effective nimodipine dose for 100 mu M kainic acid neurotoxicity was 200 mu M (P = 0.000) while 50 and 100 mu M concentrations of nimodipine were found ineffective. In this study, we have proven the dose-dependent neuroprotective role of nimodipine in kainate induced neurotoxicity in cerebellar granular cell cultures of rat pups
Atypical clinical form of cutaneous leishmaniasis: erysipeloid form
No full textA 60-year-old woman was admitted to our clinic with a gradually enlarging red papule on her face. Her history revealed that, 9 months previously, a painless, red papule of 1-2 cm in diameter had occurred in the middle of her face and, with time, had enlarged to cover her nose, both cheeks, and eyelids. It was diagnosed as a superficial skin infection, and topical and systemic antibiotics were prescribed; however, no response was obtained. In the last 2 months, a sore had formed in the middle of her nose. The patient lives in the east of Eastern Anatolia, where no case of cutaneous leishmaniasis has been reported in the last 20 years. On dermatologic examination of the patient, an erythematous, indurated, slightly squamous, 10 cm x 5 cm, butterfly-shaped plaque with sharply defined borders was seen on both cheeks, lower eyelids, and the whole nose (Fig. 1). In smears taken from the lesion, a number of amastigotes belonging to Leishmania were determined in the intracellular and extracellular area (Fig. 2). Histopathologic examination of the cutaneous lesion showed scattered infiltration composed of mononuclear cells, histiocytes, plasma cells, and small epithelioid granulomas. Gram smear and anaerobe-aerobe culture prepared from the lesion were negative. The total blood count and sedimentation rate of the patient were within normal limits. Routine biochemical tests, urine analysis, chest radiography, and intradermal purified protein derivative (PPD) skin test were all normal. Antinuclear antibody and antistreptolysin antibody examinations were negative. The patient was treated intramuscularly with 10 mg/kg/day systemic meglumine antimoniate, divided into two doses, for 20 days, with good control
The effects of genistein supplementation on fructose induced insulin resistance, oxidative stress and inflammation
No full textAims: This experimental study was designed to investigate the effects of 10 weeks genistein administration on oxidative stress and inflammation in serum and liver of rats fed with fructose. Main methods: 6-8 weeks old, 40 male Sprague-Dawley rats were included. Group 1 (control) was fed with standard chow food and 100 1/kg/day/rat dimethyl sulfoxide (DMSO) administered subcutaneously; group 2 (genistein) with standard chow food and 025 mg/kg/day/rat genistein; group 3 (fructose) with standard chow food and drinking water 20% fructose, group 4 (fructose + genistein) with standard chow food, drinking water with 20% fructose and 025 mg/kg/day/rat genistein. TNF-alpha, IL-6, visfatin as inflammatory markers and 8-isoprostane as a oxidative stress marker were measured by ELISA, glucose, triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol by enzymatic calorimetric method, AST and ALT by kinetic UV method. Key findings: Significantly high 8-isoprostane levels in serum (p < 0.001) and liver (p < 0.05) in group 3 compared to control group indicate that presence of oxidative stress. Significantly high TNF-alpha and IL-6 levels in serum (p < 0.05) and liver (p < 0.01) and visfatin levels in serum (p < 0.001) of group 3 indicate inflammation accompanying insulin resistance and oxidative stress. Genistein administration to fructose group causes a significant decrease in HOMA-IR (p < 0.001) and LDLC (p < 0.05) level. Significantly lower serum 8-isoprostane (p < 0.01) level indicates the antioxidant effect of genistein and significantly lower liver TNF-alpha (p < 0.01), serum, liver IL-6(p < 0.01) and serum visfatin (p < 0.01) levels reflect the antiinflammatory effects of genistein. Significance: Genistein administration to rats fed with fructose causes an ameliorating effect on HOMA-IR values and lipid status markers in addition to its antioxidant and antiinflammatory effects. (C) 2016 Elsevier Inc. All rights reserved
Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis
No full textThe aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60, and 2.85 ± 0.47 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively, and mean glutathione peroxidase activity was 0.72 ± 0.25, 0.33 ± 0.09, 0.37 ± 0.04, 0.34 ± 0.07 and 0.42 ± 0.1 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Cerulein-induced liver damage was accompanied by a significant increase in tissue malondialdehyde levels (P < 0.05) and a significant decrease in catalase (P < 0.05) and GPx activities (P < 0.05). L-arginine and pentoxifylline, but not L-NAME, protected against this damage. Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancreatitis-induced hepatic damage
Ultrastructural clues for the protective effect of melatonin against oxidative damage in cerulein-induced pancreatitis
No full textThe role of oxidative stress has been evaluated in experimental models of acute pancreatitis (AP). The aim of this study is to investigate the effect of melatonin on the ultrastructural changes in cerulein-induced AP in rats. Acute pancreatitis was induced by two i.p. injections of cerulein at 2-hr intervals (50 mu g/kg BW). One group received additionally melatonin (20 mg/kg BW) i.p. before each injection of cerulein. The rats were sacrificed 12 hr after the last injection. Pancreatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides and changes in the antioxidant enzyme levels, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total glutathione (GSH) levels. Ultrastructural examination was performed using a transmission electron microscope. Formation of numerous, large autophagosomes, mitochondrial damage, dilatation of rough endoplasmic reticulum (RER) and Golgi apparatus, margination and clumping of nuclear chromatin were the major ultrastructural alterations observed in the AP group. Melatonin administration prevented mitochondrial and nuclear changes and dilatation of RER and Golgi apparatus. Rare, small autophagosomes were present within the cytoplasm of some of the acinar cells. Pancreatic damage was accompanied by a significant increase in tissue MDA levels (P < 0.05) and a significant decrease in CAT, SOD, GPx activities and GSH levels (P < 0.005). Melatonin administration significantly reduced MDA levels but increased CAT, SOD, GPx activities and GSH levels (P < 0.005). Melatonin also reduced serum amylase and lipase activities, which were significantly elevated in AP (P < 0.05 and P < 0.005 respectively). These results suggest that oxidative injury is important in the pathogenesis of AP. Melatonin is potentially capable of limiting pancreatic damage produced during AP by protecting the fine structure of acinar cells and tissue antioxidant enzyme activities
Serum interleukin-2, interleukin-6, tumour necrosis factor-alpha and nitric oxide levels in patients with Behçet's disease
No full textIntroduction: Behçet's disease (BD) is a chronic systemic disorder characterised by oral and genital ulcerative lesions, ocular and cutaneous manifestations. Cytokines are the major mediators of immunologic and inflammatory reactions. Nitric oxide is reactive nitrogen intermediate which plays a key role in pathogenesis of many inflammatory and autoimmune skin diseases. The study was conducted to determine serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumour necrosis factor (TNF)-alpha and nitric oxide levels in relation to the pathogenesis of Behçet's disease. Materials and Methods: Serum IL-2, IL-6, and TNF-alpha levels were measured with test kits by enzyme-linked immunosorbent assay (ELISA) method, while serum nitric oxide levels were determined with a test kit by colorimetric method. Serum IL-2, IL-6, TNF-alpha and nitric oxide concentrations in 27 patients with Behçet's disease and in 16 healthy controls were determined by extrapolation from their standard curves. The significance of the mean differences between the 2 groups was assessed by the Mann-Whitney U test. Results: The serum levels of IL-2, IL-6, TNF-alpha, and nitric oxide concentrations in patients with BD were significantly higher than those of the controls (P <0.001). Conclusion: Our results suggest that elevated levels of IL-2, IL-6, TNF-alpha, and nitric oxide in Behçet's disease appear to be related to the disease
