167,770 research outputs found
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
MS
No full textthesisHuman tRNA(Lys,3) is the sole primer of HIV reverse transcription. The interaction between the tRNA and HIV RNA was studied by using E. coli tRNA(Lys) as a model. A reversed phase C4 method was developed for fractionation of tRNAs. This method was used to isolate (15)N labeled tRNA(Lys) from E. coli grown in minimal medium. The modification status of the isolated tRNA was verified by RNase Tl digestion followed by LC/MS. The isolated tRNA(Lys) was characterized by NMR. Data from (1)H and (1)H-(15)N spectroscopy were used to assign residues in the tRNA. An RNA construct based on the HIV Mai sequence was designed with sequence modifications to favor binding to E.coli tRNA(Lys). The construct was designated M3EC. The construct contained highly conserved elements of the viral RNA that are known to interact with the tRNA primer during reverse transcription. M3EC was transcribed in vitro from a DNA template by using rNTPs and T7 RNA polymerase. The tRNA-M3EC complex was formed by heat annealing. Specific binding of MSEC to E. coli tRNA(Lys) and complex formation was analyzed by an electrophoretic mobility shift assay. The tRNA(Lys)-M3EC complex was characterized by NMR. Residues in the complex were assigned by using data from (1)H and (1)H-(15)N HSQC experiments. Evidence for extensive interaction between the two RNA molecules was seen. Some tertiary interactions in the free tRNA were disrupted. Extensive disruption of base pairs in the acceptor and T?C stems was also observed. These bases formed new base pairs with the M3bC residues, as evidenced by appearance of new peaks m the HSQC spectrum. There was also proof of interaction between the A-loop and the tRNA anticodon. The NH11 proton of t(6)^A37 in the anticodon gave two signals in the complex, resulting from bound and free states. The change in chemical shift of the t(6)A proton indicates that modified nucleotides in the anticodon play a significant role in the A-loop/anticodon interaction. It also supports the kissing hairpin model proposed for the interaction
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
Molecular Characterization Of Hemoglobins Kurosaki [α7 Lys→glu], G-pest [α74 Asp→asn], Stanleyville-ii [α78 Asn→lys] And J-rovigo [α53 Ala→asp]
No full text[No abstract available]1024203205Bunn, H.F., Forget, B.G., Hemoglobin: Molecular, Genetic and Clinical Aspects, p. 1986. , Philadelphia, SaundersCotton, R.G.H., (1997) Mutation Detection, , Oxford, Oxford University PressDaeie, J.V., Lewis, S.M., (1995) Practical Haematology, Ed 8, , London, Churchill LivingstoneAlter, B.P., Goff, S.C., Efremov, G.D., Gravely, M.E., Huisman, T.H.J., Globin chain electrophoresis: A new approach to determination of γ G/γ A ratio of globin synthesis (1980) Br J Haematol, 44, pp. 527-534Dodé, C., Rochette, J., Krishnamoorthy, R., Locus assignment of human α-mutations by selective amplification and direct sequencing (1990) Br J Haematol, 76, pp. 275-281Thein, S.L., Hinton, J., A single and rapid method of direct sequencing using dynabeads (1991) Br J Haematol, 79, pp. 113-115Baysal, E., Huisman, T.H.J., Detection of common deletion α-thalassemia-2 determinants by PCR (1994) Am J Haematol, 46, pp. 208-213Dodé, C., Krishnamoorthy, R., Lamb, J., Rochette, J., Rapid analysis of -α 3,7 thalassaemia and ααα anti 3,7 triplication by enzymatic amplification analysis (1993) Br J Haematol, 83, pp. 105-111Harano, T., Harano, K., Imai, K., Murakami, T., Matsuhara, H., Hb Kurosaki [α7(A5) Lys → Glu]: A new α chain variant found in a Japanese woman (1995) Hemoglobin, 19, pp. 197-201Brimhall, B., Duerst, M., Hollan, S.R., Stenzel, P., Szelényl, J., Jones, R.T., Structural characterization of hemoglobin J-Buda [α61 (E10) Lys → Asn] and G-Pest [α74(EF3) Asp → Asn] (1974) Biochim Biophys Acta, 336, pp. 344-360Costa, F.F., Sonati, M.F., Zago, M.A., Hb stanleyville-II (α 278 Asn → Lys) is associated with a -α 3,7kb α-globin gene deletion (1991) Hum Genet, 86, pp. 319-320Alberti, R., Mariuzzi, G.M., Artibani, L., Bruni, E., Tentori, L., A new haemoglobin variant: J-Rovigo alpha 53 (E-2) alanine → aspartic acid (1974) Biochim Biophys Acta, 342, pp. 1-
Growth Hormone (GH)-Releasing Peptide Stimulation of GH Release from Human Somatotroph Adenoma Cells: Interaction with GH-Releasing Hormone, Thyrotropin- Releasing Hormone, and Octreotide.
No full textThe synthetic hexapeptide GH-releasing peptide (GHRP; His-D-Trp-Ala-Trp-D-Phe-Lys-NH2) specifically stimulates GH secretion in humans in vivo and in animals in vitro and in vivo via a still unknown receptor and mechanism. To determine the effect of GHRP on human somatotroph cells in vitro, we stimulated cell cultures derived from 12 different human somatotroph adenomas with GHRP alone and in combination with GH-releasing hormone (GHRH), TRH, and the somatostatin analog octreotide. GH secretion of all 12 adenoma cultures could be stimulated with GHRP, whereas GHRH was active only in 6 adenoma cultures. In GHRH-responsive cell cultures, simultaneous application of GHRH and GHRP had an additive effect on GH secretion. TRH stimulated GH release in 4 of 7 adenoma cultures; in TRH-responsive cell cultures there was also an additive effect of GHRP and TRH on GH secretion. In 5 of 9 adenoma cultures investigated, octreotide inhibited basal GH secretion. In these cell cultures, GHRP-induced GH release was suppressed by octreotide. In 5 of 5 cases, the protein kinase-C inhibitor phloretin partly inhibited GHRP-stimulated GH release, but not basal GH secretion. In summary, GH secretion was stimulated by GHRP in all somatotroph adenomas investigated, indicating that its unknown receptor and signaling pathway are expressed more consistently in somatotroph adenoma cells than those for GHRH, TRH, and somatostatin. Our data give further evidence that GHRP-stimulated GH secretion is mediated by a receptor different from that for GHRH or TRH, respectively, and that protein kinase-C is involved in the signal transduction pathway. Because human somatotroph adenoma cell cultures respond differently to various neuropeptides (GHRH, TRH, somatostatin, and others), they provide a model for further investigation of the mechanism of action of GHRP-induced GH secretion
Radio singer Gogo De Lys.
No full textRadio singer Gogo De Lys. From the back: "'Blues-Singing Gabrielle' Gabrielle De Lys, the warm-voiced singer who's heard on the Armour Program with Phil Baker every Friday over an NBC-WJZ network at 9:30 p.m., EST. She's French-Canadian, got her start in radio on the West coast and came East recently for the Amour engagement."To order a reproduction, inquire about permissions, or for information about prices see:
http://www.lib.washington.edu/specialcollections/services/reproduction/reproduction
Please cite the Order NumberScanned at 600ppi with an Epson 20000 flatbed scanner. Image then rotated, cropped, level-adjusted, and sharpened using Photoshop CS3. Converted to a JPEG2000 image upon ingest into CONTENTdm
Balzac, Le lys dans la vallée, Édition de M. Le Yaouanc
No full textM. J. Balzac, Le lys dans la vallée, Édition de M. Le Yaouanc. In: Bulletin de l'Association Guillaume Budé, n°3, octobre 1966. p. 394
Extracellular aspartic protease SAP2 of Candida albicans yeast cleaves human kininogens and releases proinflammatory peptides, Met-Lys-bradykinin and des-Arg(9)-Met-Lys-bradykinin
No full textBradykinin-related peptides, universal mediators of inflammation collectively referred to as the kinins, are often produced in excessive amounts during microbial infections. We have recently shown that the yeast Candida albicans, the major fungal pathogen to humans, can exploit two mechanisms to enhance kinin levels at the sites of candidial infection, one depending on adsorption and activation of the endogenous kinin-generating system of the host on the fungal cell wall and the other relying on cleavage of kinin precursors, the kininogens, by pathogen-secreted proteases. This work aimed at assigning this kininogenase activity to the major secreted aspartic protease of C. albicans (SAP2). The purified SAP2 was shown to cleave human kininogens, preferably the low molecular mass form (LK) and optimally in an acidic environment (pH 3.5-4.0), and to produce two kinins, Met-Lys-bradykinin and its derivative, {[}Hydroxyproline(3)]-Met-Lys-bradykinin, both of which are capable of interacting with cellular bradykinin receptors of the B2 subtype. Additionally, albeit with a lower yield, des-Arg(9)-Met-Lys-bradykinin, an effective agonist of B1-subtype receptors, was released. The pathophysiological potential of these kinins and des-Arg-kinin was also proven by presenting their ability to stimulate human promonocytic cells U937 to release proinflammatory interleukin 1 beta (IL-1 beta) and IL-6
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