6,567 research outputs found
Radyoterapiyi takiben beyaz kan hücrelerindeki morfolojik etkiler ve mikronukleus s kl
Get PDFBu çal mada, akci er tümörleri nedeniyle radyoterapiye (RT) maruz kalan kanser hastalar n n periferal kan örneklerindeki morfolojik hasarlar ve mikronukleus (MN) s kl n belirlemeye çal t k. Bu amaçla, in vivo kümülatif doz-etki ili kisini kulland k ve bu verileri istatistiksel parametreler ile ili kilendirdik. Sitolojik analizler, ? radyasyonuna maruz kalan hastalar n beyaz kan hücrelerinde (WBC) gerçekle tirildi. MN testi, radyasyon hasar n n bir belirteci olarak kullan ld . Ayr ca, sigara al kanl ve ya gibi farkl faktörlerin etkileride MN testi kullan larak ara t r ld . MN say m , tek nükleuslu lenfositlerde gerçekle tirildi. WBC'nin say s ve morfolojik özelliklerini belirlemek amac yla periferal kan yayma preparatlar haz rland . Sonuçlar, ? radyasyonuna maruz kalan kanser hastalar n n WBC'deki MN s kl ve morfolojik hasarlar n, kontrol grubundakilerden oldukça yüksek oldu unu gösterdi ve fark istatistiksel olarak önemliydi (P0.01). WBC'de voküolizasyon, membran kusurlar ve sitoplazmik granülasyonda art gibi morfolojik de i meler belirlendi. Ayr ca, radyasyon dozuna ba l olarak MN s kl nda bir art ve WBC'nin say s nda ise bir azalma gözlendi. Sonuç olarak, morfolojik hasarlar ve MN s kl n n, RT 'nin etkilerini ara t rmak için çok hassas ve kullan l biyolojik belirteçler oldu u gösterildi.In this study, we tried to define the frequency of micronuclei (MN) and morphological damages in peripheral blood samples of cancer patients exposed to radiotherapy (RT) for lung tumors. For this purpose, we used in vivo cumulative dose-effect relationship, and correlated these data with statistical parameters. Cytological analyses were performed on white blood cells (WBC) of patients exposed to ? radiation. MN assay was used as a biomarker of radiation damage. Besides, the effect of different factors like smoking status and age was investigated by MN assay. Scoring of MN was performed on mononucleated lymphocytes. Peripheral blood smears were prepared for morphological characterization and the number of WBC. The results indicated that, morphological damages and MN frequency in WBC were significantly higher in cancer patients exposed to ? radiation than in the controls, and difference was statistically significant (P<0.01). The morphological changes such as vacuolization, membrane defects and increase in cytoplasmic granulation were detected in WBC. Moreover, an increase in the frequency of MN and a decrease in the numbers of WBC were observed depending on radiation dose. In conclusion, it was showed that morphological damages and MN frequency are very sensitive and useful biomarkers for the investigation of the effects of RT
Study on the molecular typing of shigellosis outbreak at a primary school in Kuanhsi, Hsinchu County
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The synthesis and characterization of a porphyrazine bearing aza-15-crown-5 moieties in the peripheral positions and its cobalt(II) complex
No full textBiyiklioglu, Zekeriya/0000-0001-5138-214XWOS: 000253205200007The synthesis of novel metal-free (H-2-Pz) and metalloporphyrazines (MgPz and CoPz), peripherally substituted with eight aza-15-crown-5 moieties, is described. The novel compounds were characterized by elemental analysis, i.r, H-1 and C-13-n.m.r, UV-vis and m.s. spectral data
The synthesis and characterization of a new (E,E)-dioxime containing 18-membered dithiadiazadioxamacrocyclic moieties and its mononuclear complexes
No full textBiyiklioglu, Zekeriya/0000-0001-5138-214XWOS: 000253205200003A new (E,E)-dioxime, (21Z,22Z)-6,7,9,10,12,13-hexahydro-16H- benzo[h][1,4,7,16,10,13][1, 4,7,16,10,13]dioxadithiadiazacyclooctadecine-21,22(20H,23H)-dione dioxime (H (2) L) has been synthesized by reacting cyanogen-di-N-oxide (2) with (2-{[2-(2-{2-[(7-aminocyclohepta-1,4,6-trien-1-yl)thio]ethoxy}ethoxy)ethyl]thio}phenyl)amine. Mononuclear complexes of this ligand have been synthesized by reacting the vic-dioxime (H2L) with CuCl2, NiCl2.6H(2)O and CoCl2.6H(2)O respectively. The BF2+ capped Ni(II) and Co(III) complexes of the dioxime have been synthesized from. The new compounds were characterized by a combination of elemental analysis, H-1- and C-13-n.m.r, I.R. and m.s. spectral data
Changes of serum biochemistry profiles in <i>Dmp1<sup>−/−</sup></i>, <i>kl/kl</i> and the <i>Dmp1<sup>−/−</sup> kl/kl</i> mice.
No full text<p>(<b>A</b>) Serum Phosphate levels were significantly lower in <i>Dmp1<sup>−/−</sup></i> mice (P<0.001), while there was a significant increase in <i>kl/kl</i> and the <i>Dmp1<sup>−/−</sup>kl/kl</i> groups (P<0.001) compared to WT (n = 7 in each group). (<b>B</b>) Serum calcium levels were statistically unchanged in <i>Dmp1<sup>−/−</sup></i> and <i>kl/kl</i> mice. However, the calcium level was significantly higher in the <i>Dmp1<sup>−/−</sup>kl/kl</i> mice than that of the WT (P = 0.04). (<b>C</b>) Serum PTH level was over 10 fold increased in <i>Dmp1<sup>−/−</sup></i> but significantly lower in both <i>kl/kl</i> and the <i>Dmp1<sup>−/−</sup> kl/kl</i> mice compared to WT (P = 0.025, and P = 0.002 respectively). (<b>D</b>) Serum 1,25(OH)<sub>2</sub>D<sub>3</sub> levels were overall higher in all these deficient mice, although only <i>kl/kl</i> mice showed significant difference compared to the WT (P = 0.043). <b>(E)</b> Serum FGF23 levels were high in all three deficient mice with <i>kl/kl</i> the highest compared to the WT control. *P<0.05 compared to WT, § P<0.05 compared to <i>Dmp1<sup>−/−</sup></i>, ¥ P<0.05 compared to <i>kl/kl</i> mice.</p
Usefulness of quantifying serum KL-6 levels in the follow-up of uveitic patients with sarcoidosis
Get PDFBackground: KL-6 is a human glycoprotein secreted by type II alveolar cells in the lung, and its serum levels increase in pneumonia of various causes. We previously reported that serum KL-6 levels in uveitis patients with sarcoidosis were significantly higher than those of other uveitis patients and healthy controls. Additionally, the combined measurement of serum KL-6 and angiotensin converting enzyme (ACE) was useful for screening uveitic patients to diagnose sarcoidosis. The purpose of the present study was to investigate the clinical usefulness of quantifying serum KL-6 levels for following-up the patients with sarcoidosis. Methods: Sera were obtained from 36 uveitic patients diagnosed with sarcoidosis and the same number of healthy volunteers. To examine the influence of systemic medication, we also collected blood samples from four more sarcoidosis patients, who were systemically treated with corticosteroid or angiotensin converting enzyme (ACE) inhibitor, an anti-hypertensive drug. The serum concentration of KL-6 was measured by a human KL-6 electrochemiluminescence immunoassay (ECLIA). Results: The mean KL-6 concentrations of sarcoidosis patients and healthy controls were 449.3 +/- 66.3 (mean +/- SE) and 192.1 +/- 11.3, respectively. The average levels of serum KL-6 were significantly elevated in sarcoidosis patients compared with healthy control subjects (P < 0.001), and there were significant correlations between serum KL-6 and ACE levels in the patients with sarcoidosis (r=0.70 and P < 0.0001). Moreover, serum KL-6 concentrations were less affected by systemic corticosteroid, and unaffected by ACE inhibitory drugs in contrast to ACE levels. Conclusions: Measurement of serum KL-6 in the uveitic patients may be useful to follow-up the diagnosed sarcoidosis, as well as for diagnosing sarcoidosis, because the serum KL-6 level was well correlated with the ACE level, and less affected by systemic medication than ACE levels
Macroscopic phenotype of <i>Dmp1/Klotho</i> compound deficient mice (<i>Dmp1<sup>−/−</sup>kl/kl</i>).
No full text<p>(A) Gross appearance of Wild Type (WT), Dmp1 null <i>(Dmp1<sup>−/−</sup>)</i>, Klotho deficient <i>(kl/kl)</i>, and compound deficient <i>(Dmp1<sup>−/−</sup>kl/kl)</i> mice at 6 weeks of age. (<b>B</b>) Body weights of 6 weeks old mice, showing a decrease in <i>Dmp1<sup>−/−</sup></i> (13%), <i>kl/kl</i> (57%) and <i>Dmp1<sup>−/−</sup>kl/kl</i> (36%) mice compared to the age matched WT (P = 0.02; P<0.001, and P<0.001 respectively; n = 10 in each group). Note that the <i>Dmp1<sup>−/−</sup>kl/kl</i> body weight was ∼ 30% higher than that of the single <i>kl/kl</i> mice (P = 0.03). (<b>C</b>) Survival for WT, <i>Dmp1<sup>−/−</sup>, kl/kl</i>, and <i>Dmp1<sup>−/−</sup>kl/kl</i> mice (n = 10 in each group). Both <i>kl/kl</i> and <i>Dmp1<sup>−/−</sup>kl/kl</i> mice showed similar low survival rate compared to WT and <i>Dmp1<sup>−/−</sup></i> groups. About 40% of <i>Dmp1<sup>−/−</sup>kl/kl</i> animals survived 3 weeks more than single <i>kl/kl</i>. Neither of the kl/kl nor <i>Dmp1<sup>−/−</sup>kl/kl</i> groups survived more than 15 weeks. None of WT and <i>Dmp1<sup>−/−</sup></i> animals died in 30 weeks of observation period. These mice were of similar genetic backgrounds but not necessarily littermates. *P<0.05 compared to WT; § P<0.05 compared to <i>kl/kl</i>.</p
Sharply increased ectopic calcification and apoptosis in the compound deficient (<i>Dmp1<sup>−/−</sup> kl/kl</i>) mice.
No full text<p>(<b>A</b>) Representative X-ray images of kidney and aorta in four groups show moderate ectopic calcifications in the <i>kl/kl</i> kidney and aorta, and massive ectopic calcification in the <i>Dmp1<sup>−/−</sup>kl/kl</i> kidney and aorta. (<b>B</b>) von-Kossa stain (left panel) and TUNEL assay (right panel) of the <i>Dmp1<sup>−/−</sup>kl/kl</i> kidney showing a close correlation between the calcified- and apoptotic cells. (<b>C</b>) A similar linkage between the ectopic calcification (von-Kossa stain; right panel) and the apoptosis (TUNEL assay; right panel) was observed in the adjacent <i>Dmp1<sup>−/−</sup>kl/kl</i> aorta. (<b>D</b>) The quantitative mineral content is sharply increased in both the <i>kl/kl</i> and the <i>Dmp1<sup>−/−</sup>kl/kl</i> kidney by µCT analysis with significantly higher in the <i>Dmp1<sup>−/−</sup>kl/kl</i> kidney than that of the single kl/kl kidney (P = 0.041). Values are means ± SEM of 3–5 kidneys. Note that there was no calcification in the WT and the <i>Dmp1<sup>−/−</sup></i> mice. (<b>E</b>) Representative DMP1 Western blots with WT at left panel and <i>kl/kl</i> at right panel shows higher density of DMP1 in <i>kl/kl.</i> Osteocalcin (OCN) is the matrix protein which does not show difference between these two groups. (<b>F</b>) Quantitative kidney RT-PCR data showing over two fold-increases of <i>Dmp1</i> mRANA in <i>kl/kl</i> compared to the WT control. n = 3, *P<0.05.</p
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