1,721,304 research outputs found
Mitochondria and metabolism: the role of SAGA complex in budding yeast respiration
Full text linkMitochondrion is the energy center of the cell in which different metabolic pathways converge. Cell respiration takes place in this organelle with high production of ATP. Nucleus-mitochondrion communication represents an essential level of cell homeostasis regulation. This crosstalk is strictly related to epigenetic mechanisms, in particular to histone and non-histone proteins acetylation. Budding yeast is a suitable model system to study the interplay between epigenetic and metabolism; in this organism it is possible to analyse fermentative and respiratory pathways varying the carbon source in growth medium. SAGA complex is a transcriptional co-activator with two catalytic domains: the deubiquitination module with DUB Ubp8 and the acetylation domain with HAT Gcn5. In yeast, Gcn5 has a major role in protein acetylation at global and specific lysine; moreover, it is implicated in mitochondrion-nucleus communication. In this work, we found that SAGA complex and its catalytic activities are necessary for cell respiration. Results showed that KAT Gcn5 and DUB Ubp8 are upregulated in respiration at both transcriptional and protein levels. We identify two interactors, the E3 ubiquitin ligase Psh1 and the HDAC Hda1, whose deletion is able to rescue defective respiratory phenotype of strains lacking respectively Ubp8 and Gcn5. We demonstrated that histone residue H3K18 is the preferential target of Hda1 and Gcn5 and its acetylation is needed in respiration. Surprising, we found that Gcn5, Ubp8 and Psh1 localize in both nucleus and mitochondria highlighting a dual role as chromatin modifiers and mitochondrial factors. Moreover, our data showed that Ubp8 expression is regulated by Gcn5, while its cell localization is influenced by Psh1. Finally, results suggest that acetylation is an important mark in respiratory condition
La rintracciabilità del latte e derivati. Giornata di studio “Rintracciabilità di filiera nelle produzioni animali per la sicurezza alimentare”
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Comparison between impedometry and bioluminescence in the investigation of microorganisms growth
No full textPectus excavatum: le alterazioni cardiorespiratorie come indicatori per l’intervento correttivo e come elementi di valutazione dei risultati.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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