5,512 research outputs found
Cd-regulated gene expression in Datura innoxia
No full textThe effects of Cd on the expression of specific proteins and transcripts have been examined in Cd-resistant (Cd300) and Cd-sensitive cultures of Datura innoxia. 2D PAGE analysis of the soluble fraction of D. innoxia protein proved problematic. Proteins precipitated on the surface of lEF gels. This was overcome by loading the samples in the gel mixture prior to polymerisation. Polymerisation of gels containing protein extracted from Cd-exposed cells only occurred when PCs, Cd and other components were resolved from the proteins eluting in the void volume following fractionation by gel filtration chromatography (Sephadex G50). Two peptides, designated Cd-1 and Cd-2, were detected in the Cd300 cells only after exposure to Cd. Cd-1 and Cd-2 were also both detected following exposure of the Cd300 cells to 125 piM Cu or Zn, or HS (42 ˚C 4 h). Neither Cd-1 nor Cd-2 were observed in protein extracts from WDI cells exposed to 125 μM Cd for 8 h. Both Cd-1 and Cd-2 proved refractory to Edman degradation while the N-terminal 30 amino acids of a third, constitutively expressed protein, designated Protein-3, were determined using equivalent procedures. This protein showed sequence similarity to PR proteins. Although cleavage of Cd-1 and Cd-2 generated polypeptides which were not terminally blocked, no sequence information was obtained from these polypeptides, even following purification using standard techniques. Oligonucleotide primers designed from the amino acid sequence of Protein-3 were successfully used to amplify, from cDNA, a fragment which was cloned, sequenced and shown to encode the characterised protein. A longer fragment was also amplified from cDNA by RACE PGR. However, this product was not cloned. In order to identify cDNA sequences encoding Cd-1 and Cd-2 an expression cDNA library was prepared and antibodies raised against the two peptides. However, no antigenicity could be detected when antisera raised against Cd-1 or Cd-2, or the purified IgG fractions, were used to probe western blots. The XZAP cDNA (Cd-exposed) library was "differentially screened" in order to isolate clones corresponding to Cd-induced genes. This led to the isolation of two Cd- induced clones designated Cd-6 (949 bp) and Cd-8 (659 bp). Both clones hybridised to transcripts of approximately 900 bp. Transcripts were also detected in RNA samples extracted from D. innoxia exposed to HS (42 ˚C 4 h), however no transcripts were detected in WDI cells exposed to Cd. Southern blots revealed hybridisation to multiple bands, possibly indicating the presence of a gene family. A motif, C-C-X-C-C, found in the a-domain of metallothioneins, was identified in Cd-6. This may represent a putative metal binding site in Cd-6
STD890726 Supplemental Material - Supplemental material for HIV self-tests for men who have sex with men, accessed via a digital vending machine: a qualitative study of acceptability
No full textSupplemental material, STD890726 Supplemental Material for HIV self-tests for men who have sex with men, accessed via a digital vending machine: a qualitative study of acceptability by S Raffe, A Pollard, JH Vera, S Soni, C Peralta, L Rodriguez, G Dean and CD Llewellyn in International Journal of STD & AIDS</p
Author Co-Citation Analysis (ACA): a powerful tool for representing implicit knowledge of scholar knowledge workers
No full textIn the last decade, knowledge has emerged as one of the most important and valuable organizational assets. Gradually this importance caused to emergence of new discipline entitled ―knowledge management‖. However one of the major challenges of knowledge management is conversion implicit or tacit knowledge to explicit knowledge. Thus Making knowledge visible so that it can be better accessed, discussed, valued or generally managed is a long-standing objective in knowledge management. Accordingly in this paper author co- citation analysis (ACA) will be proposed as an efficient technique of knowledge visualization in academia (Scholar knowledge workers)
Portfolio of recorded performances and exegesis: Messiaen’s musical language for the jazz pianist - an exploration through performance.
No full textMoving beyond Gunther Schuller’s Third Stream amalgamation of classical and jazz, this study explores whether the musical language of Olivier Messiaen can make a valid contribution to jazz piano performance. Initially, my project sought to answer such questions as: What elements of the musical language of Messiaen already exist in the jazz vocabulary? Am I able to extend this further? What are the timbral structures and pianistic effects within Messiaen’s musical language? What will be the most effective application of Messiaen’s musical language to jazz piano performance? Endeavouring to answer the final question led me to consider such aspects as whether the project should be limited to quoting Messiaen motifs, arranging Messiaen melodies, replacing jazz harmonic structures on standards with examples from Messiaen’s musical language or whether it would be better to approach the research conceptually. The work of Hubert Nuss provided encouraging reassurance that this was not an impossible task. In order to articulate this conception, the initial challenge was to decide how the classical and jazz worlds might meet in a ‘Messiaen’ technique. The approach adopted was similar to that used for undergraduate jazz study, namely, immersion in the piano scores and recordings of Messiaen’s music as well as by live performances. This was followed by the development and assessment of a contrived approach when specific techniques, such as tonal colourings or harmonic structures, were developed through prepared exercises and consciously included in my performance. It was then compared with an intuitive approach when no such precise parameters were established. This submission consists of CD recordings of two public recitals and an exegesis. It documents the development of this Messiaen technique and discusses its application in my performances. It also demonstrates the ways that Messiaen’s musical language can be used within jazz piano performance to provide a colour that distinguishes jazz piano performance in a competitive field.Thesis (M.Mus.) -- University of Adelaide, Elder Conservatorium of Music, 201
Spinal anaesthesia for brachytherapy for carcinoma of the cervix a comparison of two dose regimes of hypebaric bupivacaine
No full textIncludes abstract.Includes bibliographical references.The main purpose of the study was to help establish the best dose regimen of hyperbaric bupivacaine, when combined with intrathecal fentanyl, for spinal anaesthesia for brachytherapy for carcinoma of the cervix. This procedure is performed as a day case at Groote Schuur Hospital
What is it about STI research that is unappealing? An experience of conducting sexual health research in general practice in England
No full textDeveloping sexual health services in primary care is recommended as a way of improving patient choice and increasing the capacity of testing services.1 ,2 Before implementing such service provisions it is essential to understand the requirements of patients. We have recently completed a survey study of sexually transmitted infection (STI) testing service users preferences for STI testing services (Miners AH, Llewellyn CD, Pollard A, et al, unpublished data, 2012). In order to have greater generalisability, we recruited patients from general practice, contraception and sexual health services and genito-urinary medicine clinics
Free books to download Cambridge IGCSE Mathematics Core and Extended Coursebook with CD-ROM (Cambridge International IGCSE)
No full textRead Or Download Cambridge IGCSE Mathematics Core and Extended Coursebook with CD-ROM (Cambridge International IGCSE) Full Books By by Karen Morrison (Author), Nick Hamshaw (Author)
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