167 research outputs found
Chromosomal assignment of six genes (EIF4G3, HSP90, RBBP6, IL8, TERT, and TERC) in four species of the genus Equus
No full textWe mapped six genes (EIF4G3, HSP90, RBBP6, IL8, TERT, and TERC) on the chromosomes of Equus caballus, Equus asinus, Equus grevyi, and Equus burchelli by fluorescence in situ hybridization. Our results add six type I markers to the cytogenetic map of these species and provide new information on the comparative genomics of the genus Equus.Pamela Vidale, Francesca M. Piras, Solomon G. Nergadze, Livia Bertoni, Andrea Verini-Supplizi, David Adelson, Gérard Guérin and Elena Giulott
Assignment of the Equus caballus interleukin 8 gene (IL8) to chromosome 3q14.2-> q14.3 by in situ hybridization
No full text© 2006 S. Karger AG, BaselNergadze, S.G. ; Magnani, E. ; Attolini, C. ; Bertoni, L. ; Adelson, D.L. ; Cappelli, K. ; Verini Supplizi, A. ; Giulotto, E
Selection of N-(phosphonacetyl)-L-aspartate resistant Chinese hamster mutants in the presence of the uridine uptake inhibitor dipyridamole.
No full textn mammalian cells selected in culture for resistance to PALA the CAD gene is amplified and these cells are a widely used model system to study gene amplification. Selection of resistant mutants is routinely performed in medium supplemented with dialyzed serum, because the cytotoxic effect of PALA is reversed by uridine, which is contained in serum. We have shown that in Chinese hamster cells dipyridamole reduced uridine uptake to less than 5% with limited effect on cell survival. Moreover, in medium supplemented with complete serum and 10 microM dipyridamole the toxicity of PALA was similar to that obtained in medium containing dialyzed serum. We then used 10 microM dipyridamole to inhibit uridine uptake during selection of PALA resistant colonies and found that both the frequency and the type of mutants were as those obtained in the presence of dialyzed serum. In particular, in the five mutants tested, the mechanism of resistance to PALA was amplification of the CAD gene
Fluorescence in situ hybridization with a synthetic (T2AG3)n polynucleotide detects several intrachromosomal telomere-like repeats on human chromosomes.
No full text(T2AG3) repeats comprise the telomeres of human chromosomes and also are present at interstitial locations. Using a long synthetic (T2AG3)n probe, we have localized telomere-like repeats at several internal sites on human chromosomes
Late onset of CAD gene amplification in unamplified PALA resistant Chinese hamster mutants.
No full textAmplification of the pericentromeric region of chromosome 1 in a newly established colon carcinoma cell line
No full textThe LRWZ cell line was established from an ascitic effusion of a colon adenocarcinoma. We studied the karyotype of LRWZ cells using G-banding and chromosome painting. The cell line is near triploid and is characterized by several chromosome rearrangements and pronounced intermetaphase variation. Chromosome painting probes revealed numerous labeled regions on different chromosomes, indicating that several translocations occurred during the evolution of the cell population. The 10 recurrent marker chromosomes identified (M1-M10) were derived from complex rearrangements involving up to three different chromosomes. M2 is a particularly interesting marker that originated from the amplification of the pericentromeric region of chromosome 1 and has a peculiar organization comprising five copies of the region included between 1p21 and 1q21 and is surprisingly stable: it is present in all the metaphases analyzed, has telomeric DNA at both termini, and contains one active and four inactivated centromeres. To provide insights into the molecular mechanisms that generated M2, we performed fluorescence in situ hybridization experiments using a panel of probes mapping near the centromere of chromosome 1 and three probes for different satellite sequences; the formation of chromosome M2 required the intervention of several rearrangements including unequal exchange, chromatid breakage followed by fusion of the sister chromatids, and loss of centromeric heterochromatin
Experimenting the use of value models as boundary objects in conceptual PSS design
No full textThe paper presents the results of experimental activities aiming at investigating the role of ‘value models’ as ‘boundary objects’ that facilitate cross-functional discussions in the PSS conceptual design phase. The experiment featured 6 separate sessions involving a total of 22 students in the final year of three different Master Programmes. In the sessions participants were asked to redesign an asphalt compactor and to use different types of design decision support for this task, namely value models vs. requirements checklists. The recordings from the experiment were analysed using protocol analysis to compare the behaviour of teams. The results confirm the hypothesis that, compared to traditional requirement checklists, value models emphasizes activities related to the clarification (1) of the problem domain and needs and (2) of the lifecycle aspects of a solution concept. Both results suggest that value models have the ability to facilitate cross-boundary discussion in the early phases of the PSS design process, and to act as boundary objects that provide a common platform for knowledge sharing within the cross-functional team.open access</p
"Par un ennemi qu'on sait digne de soi": la lunga traccia delle coppie antinomiche
No full textThe contribution deals with antinomic couples, actual rivalries, symbolic confrontations; it is part of a discussion among scholars about the subject, in a column edited by the author and Niccolò Scaffa
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