392,160 research outputs found
An Analysis of <i>Judge Lin</i>
No full textBiography of Lin Wen Zhong Gong has another way to call, that is Judge Lin. The leading character is Lin Ze-Xu. This book is based on functionary experience of Lin Ze-Xu, with the captivating plots of court case, helping by highly skilled military attach\uc3\ua9s and chivalrous knights, and the history facts of Opium War. It makes Lin Ze-Xu\ue2s Confucian temperament and tragic mood more, also contrasts with author\ue2s sorrow and furiousness for the politics at the time. History, court case, martial arts\ue2\ua6\ue2\ua6etc. are essence of this book and it broadens the way of this writing style.
The topic of the thesis is \ue2An Analysis of Judge Lin\ue2. The following thesis will be divided into six different chapters. The introduction is Chapter one of the thesis, which is including researching motive and purpose, literature review of predecessors, researching version by existing information, raising questions, choosing research methods and arranging chapters. In chapter Two, I discuss the study of characters of Lin Ze-Xu, also makes a deep analysis of author\u27s purpose of writing him. In chapter Three, I analyze supporting actors and actress. Meanwhile, I illustrate author\u27s purpose of writing supporting actress because the author had different manner to describe supporting actress. Moving to the Chapter Four, I mainly focus on the plots of Judge Lin, and organize cases of Lin Ze-Xu and his subordinates to understand features of cases. In Chapter Five, I represent the causes of Opium War. China and England had difference of opinions of opium. Therefore, it is easier to comprehend what the author\u27s purpose is. In the last chapter I summarize the main points of the preceding chapters and confirm particularity of Judge Lin
<i>lin-28</i> Mutants Exhibit Reduced Fertility.
No full text<p>(<b>A</b>) Schematic drawing of the germarium with its two stem cell clusters, i.e. germline stem cells (GSCs) and follicular stem cells (FSCs), and of an ovariole composed of a string of egg chambers of increasing maturation stages. The last egg chamber is composed of a large oocyte with its karyosome (kary.) at the posterior pole, and the 15 nurse cells at the anterior pole. (<b>B</b>) While <i>w<sup>1118</sup></i> female flies laid 18.8 eggs per day, <i>lin-28<sup>dF30/+</sup></i>25.1 eggs per day and <i>lin-28<sup>dF101/+</sup></i>24.8 eggs per day, <i>lin-28<sup>dF3</sup></i><sup>0</sup> and <i>lin-28<sup>dF101</sup></i> mutant flies laid respectively 5.5 and 5.2 eggs per day (n = 10; p<0.05). (<b>C</b>) Comparison of whole ovaries revealed increased auto-fluorescence in homozygous ovaries. The auto-fluorescence is localized at the oocyte portion of the eggs (asterisk), therefore indicating an increased number of eggs between stages 7 to 10, compared to controls. In addition, the visualization of nuclei in single ovarioles revealed an accumulation of supernumerary nurse cells in <i>lin-28<sup>dF30</sup></i> and <i>lin-28<sup>dF101</sup></i> stage 8 egg chambers. (<b>D</b>) 75.4% and 78.1% of homozygous <i>lin-28<sup>dF30</sup></i> and <i>lin-28<sup>dF101</sup></i> egg chambers displayed abnormal egg chambers, compared to 16.9% of the <i>w<sup>1118</sup></i> and 13.8% and 12.7% of the heterozygous <i>lin-28<sup>dF3</sup></i><sup>0/+ and </sup><i>lin-28<sup>dF101/+</sup></i> ovarioles (n = 65). Scale bars represent 100 µm.</p
Morpholino-mediated knockdown of <i>lin-28a</i> and <i>lin-28b</i> affects early development.
No full text<p>A. B. Schematic representation shows the genomic organization of the <i>lin-28a</i> and <i>lin-28b</i> genes in zebrafish. Regions targeted by splice-blocking morpholinos are shown. Arrows depict the location of the two primer sets to amplify exon and intron sequences, which were utilized in the RT-PCR analysis. C. D. The efficacy of MO was validated by RT-PCR using primers as indicated in A, B. Total RNA was isolated from <i>lin-28a</i>, <i>lin-28b</i> or control MO-injected embryos at 5 hpf and analyzed by using specific primers for <i>lin-28a</i> and <i>lin-28b</i>. Upper panel shows a reduced expression of <i>lin-28a</i> and <i>lin-28b mRNA</i>, and an increase of an aberrantly spliced <i>lin-28b</i> transcripts corresponding to the presence of intron 1. Lower panel shows RT-PCR products with <i>gapdh</i> primers used as internal control. E. Representative images of severe phenotype of <i>lin-28a</i> and <i>lin-28b</i> morphants at 5 hpf. F. Vegetal view of control, <i>lin-28a</i> and <i>lin-28b</i> morphants at 8 hpf. Arrow shows the similar extent of prechordal plate extension in control, <i>lin-28a</i> and <i>lin-28b</i> morphant embryos; bracket shows a reduced extent in epiboly in <i>lin-28a</i> and <i>lin-28b</i> morphant embryos compared with controls. G. Representatives of mild and severe phenotypes in <i>lin-28a</i> and <i>lin-28b</i> morphants at 30 hpf. Both <i>lin-28a</i> and <i>lin-28b</i> morphants develop morphological phenotypes displaying shorten body axis, small anterior structures and aberrant tail morphology ranging from mild (Right) to severe (Left) compare to control MO injected embryos (Top). Arrowheads indicate the smaller head. H. Quantification of the efficiency of rescue from gastrulation defects following co-injection of <i>lin-28a</i> MO, <i>lin-28b</i> MO and mRNAs. More than half of the <i>lin-28a</i> MO or <i>lin-28b</i> MO injected embryos had died by 30 hpf. The frequencies of the phenotypes were similar between <i>lin-28a</i> and <i>lin-28b</i> morphants. The frequency of the dead, mild and severe phenotypes decreased with a rescue experiment in which 300 pg of <i>lin-28a</i> and <i>lin-28b</i> mRNAs were co-injected with the MO. (p<0.0001, <i>lin-28a</i> MO vs. <i>lin-28a</i> MO+<i>lin-28a</i> mRNA, <i>lin-28b</i> MO vs. <i>lin-28b</i> MO+<i>lin-28b</i> mRNA, Fisher’s exact test). The total number of embryos is noted above each bar.</p
Espai i identitat en l'obra de Jordi Pere Cerdà. Una geografia literària cerdaniana
Full text linkL'obra de l'autor nord-català Jordi Pere Cerdà (1920-2011) teixeix una cartografia literària que abasta tota dimensió espacial -real, imaginada i ficcional. Les prospeccions que assagen els seus texts es fonen en el medi natural i rural, canten a l'amor, als veïns i als éssers fantàstics del folklore català, es comprometen amb els refugiats encarant-se a tota frontera i, també, a tot abisme interior i exterior que oprimesca l'ésser. El mapatge cognitiu i literari que crea Cerdà sobrepassa qualsevol obstacle per construir espais oberts i possibles, en comunió amb l'altre. Partint d'una aproximació teòrica geocrítica, aquest treball d'investigació aprofundeix en diverses nocions sobre l'espacialitat lligades a un context convuls, ple de transformacions a nivell socioeconòmic, polític, cultural i lingüístic, el qual determinarà la vida d'un autor i d'un territori transfronterer com el de la Cerdanya i la Catalunya del Nord. En definitiva, la rica experiència vital de Jordi Pere Cerdà ens permet reflexionar sobre les relacions que vulguem establir entre els individus i amb el nostre hàbitat natural i cultural, a fi d'esdevenir membres actius que participen de la transformació dels espais que configuren les nostres identitats.The work of the North Catalan author Jordi Pere Cerdà (1920-2011) weaves a literary cartography which reaches all spatial dimensions -real, imagined and fictional. The prospections proved by their texts merge with the natural and rural environment, sing to love, neighbours and the fantastic beings of the Catalan folklore. Such prospections also commit themselves with the refugees facing every frontier and, also, facing all interior and exterior abyss that oppresses the being. The cognitive and literary mapping created by Cerdà overcomes any obstacle to construct opened and possible spaces, in communion with the other. Based on the theoretical approach called geocriticism, this research study delves into various notions about spatiality linked to a convulsive context, full of transformations at a socioeconomic, political, cultural and linguistic level; these transformations will determine the life of an author and a cross-border territory such as Cerdagne and Northern Catalonia. In short, the rich experience of Jordi Pere Cerdà allows us to reflect on the relationships we want to establish between individuals, as well as between human groups and our natural and cultural habitat, in order to become active members that participate in the transformation of the spaces that make up our identities.Programa de Doctorat en Llengües Aplicades, Literatura i Traducci
Characterization of the <i>lin-28<sup>dF30</sup></i> Allele.
No full text<p>(<b>A</b>) The <i>lin-28<sup>dF30</sup></i> allele was created by imprecise P-element excision that resulted in a deletion of 1007 bases, upstream of the P-element insertion. (<b>B</b>) The predicted <i>lin-28<sup>dF30</sup></i> mRNA lacked the second, the third and partially the fourth exon. (<b>C</b>) The predicted Lin-28<sup>dF30</sup> protein lacked the CSD, the linker and at least one of the CCHCs. (<b>D</b>) qRT-PCR analysis of <i>Drosophila</i> ovaries, demonstrated that the control of <i>pre-let-7</i> processing is greatly affected by the deletion of <i>lin-28</i> compared to controls, resulting in a dramatic increase in the levels of mature <i>let-7</i> (from 0.17 in <i>w<sup>1118</sup></i>, 0.18 in <i>lin-28<sup>[EP915]</sup></i>, 0.21 in <i>lin-28<sup>dF30/+</sup></i> and 0.23 in <i>lin-28<sup>dF101/+</sup></i> to 0.92 in <i>lin-28<sup>dF30</sup></i> and 0.95 in <i>lin-28<sup>dF101</sup></i> mutant flies). Student t-test: p<0.05.</p
Els llibres d'il·lustració infantil i juvenil en l'Educació Artística. Un cas concret a partir de l’autora Olga de Dios
Full text linkTreball Final de Grau en Mestre o Mestra d'Educació Primària (Pla de 2018). Codi: MP1840. Curs acadèmic: 2021/2022El treball de fi de grau que aneu a veure a continuació, és un projecte que consisteix en com es poden
treballar a les aules de primària els llibres de l’autora i il·lustradora Olga de Dios. Tractant de treballar
el llibre com a objecte artístic a nivell elemental. A més, de presentar a l’autora i il·lustradora, donarla a conèixer i veure els valors que transmet, també llegiríem quatre de les seues obres a l’aula com
són: “Leotolda”, “En familia”, “Rana de Tres Ojos”, i “Pájaro Amarillo”. Tot açò sumat a la
realització d’un quadern de classe on es colorejaria alguns dels seus personatges de les obres seguint
pautes a nivell curricular. Realitzaríem també una eixida a la biblioteca municipal, on a banda de
rebre una educació no formal, ens facilitaria una mica el fet d’endinsar-nos al món de la il·lustració
infantil, on allí dins, el professional encarregat ens guiarà pels llibres que hi haurà a la biblioteca. I,
per a concloure, elaboraríem un llibre creat per l’alumnat on seguint unes pautes establertes per Olga
de Dios al seu llibre “Leotolda” buscaríem fer una retroalimentació intentant contactar amb ella.
D’aquesta manera tancaríem el cicle que vam començar donant-la a conèixer com a escriptora i
il·lustradora, i exploraríem on està el límit de la creativitat del propi alumnat.The final degree project you are going to see below is a project that looks at how books by author and
illustrator Olga de Dios can be worked on in primary school classrooms. Considering the book as an
artistic object at an elementary level. Besides presenting the author and illustrator, introducing her,
and seeing the values she transmits, we would also read four of her works in the classroom, such as:
"Leotolda”, "In the family", "Three-Eyed Frog", and "Yellow Bird.". All this, in addition to the
creation of a class notebook in which some of his characters from her work would be colored
following guidelines at the curricular level. We would also go out to the local library, where in
addition to receiving a non-formal education, it would make it a little easier for us to enter the world
of children's illustration, where inside, the professional in charge will guide us through the books
which will be in the library. And, to conclude, we would make a book created by the students, where
following some guidelines established by Olga de Dios in her book "Leotolda" we would look for
feedback by trying to contact her. In this way, we would close the cycle that we started by presenting
her as an author and illustrator, and we would explore where the limit of creativity of the students
themselves is
Les biblioteques universitàries i el suport a la recerca
Full text linkThe European Higher Education Area and the European Research Area are currently two key objectives for university learning, teaching and research in all European universities. University libraries must meet the challenges by offering the best library services to the academic community. In drafting the second strategic plan, Rebiun (Network of University Libraries) gathered comments and suggestions from professionals in Spanish university libraries. This article presents the reflections and suggestions contributed by the author regarding the support services that university libraries could offer for university research.L'espai europeu d'educació superior i l'espai europeu d'investigació són actualment dos objectius clau per a l'aprenentatge, la docència i la recerca de totes les universitats europees. Les biblioteques universitàries han de contribuir a aquests reptes, oferint els millors serveis bibliotecaris a la comunitat acadèmica. En la fase d'elaboració del seu segon Pla estratègic, la REBIUN (Red de Bibliotecas Universtarias) va recollir reflexions i suggeriments de diferents professionals de les biblioteques universitàries de l'Estat espanyol. En l¿article següent es presenten les reflexions i els suggeriments aportats per l'autora sobre el suport que les biblioteques universitàries poden oferir a la recerca universitària
Urbs Upon a Hill. Lectura intertextual de l'Eneida de Virgili i Hamilton de Lin-Manuel Miranda
Full text linkAquest treball proposa una anàlisi intertextual de l'Eneida, el poema èpic de Virgili, i Hamilton, l'obra de teatre musical de Lin-Manuel Miranda. A través de la comparació d'elements temàtics, argumentals i formals, es planteja la possibilitat d'una influència mútua en les lectures de totes dues obres. D'aquesta manera, el present treball busca equiparar un dels textos cabdals de la literatura occidental amb una peça sovint considerada de caràcter popular.This dissertation poses an intertextual analysis of the Aeneid, Virgil's epic poem, and Hamilton, Lin-Manuel Miranda's musical play. Through the comparison of thematic, plot and formal elements, the possibility of a mutual influence on the reading of both works is raised. Thus, the present dissertation seeks to equate one of the main texts in Western literature with a piece often considered of a popular nature
Expression of miRNAs and downstream heterochronic genes in <i>lin-28a</i> and <i>lin-28b</i> morphant embryos.
No full text<p>A. miRNA expression profiling in <i>lin-28a</i> and <i>lin-28b</i> morphants embryos at 5 hpf. Hierarchically clustered heat-map representing differences in miRNA expression between control MO-injected embryos and <i>lin-28a</i> MO- or <i>lin-28b</i> MO-injected embryos. Relative levels of expression are colored from green (low) to red (high). B. Real-time PCR analysis of <i>let-7a, let-7b, miR-430a</i> and <i>miR-430b</i> expression in control MO-, <i>lin-28a</i> MO- and <i>lin-28b</i> MO-injected embryos. Expression levels of <i>let-7a, let-7b, miR-430a</i> and <i>miR-430b</i> were analyzed at 5 hpf using TaqMan miRNA assay. The expression level of <i>miR-430a</i> and <i>miR-430b</i> was downregulated in both <i>lin-28a</i> MO- and <i>lin-28b</i> MO-injected embryos compared with control MO-injected embryos (***p<0.001, control MO vs. <i>lin-28a</i> MO, control MO vs. <i>lin-28b</i> MO, two-tailed test, n = 3, mean ± SEM). C. Real-time PCR analysis of <i>let-7a</i> and <i>let-7b</i> expression in control MO-, lin-28a MO- and lin-28b MO-injected embryos. Expression levels of <i>let-7a</i> and <i>let-7b</i> were analyzed using TaqMan miRNA assay at 28 hpf. The expression level of <i>let-7a</i> and <i>let-7b</i> was increased in both <i>lin-28a</i> MO- and <i>lin-28b</i> MO-injected embryos embryos compared with control MO-injected embryos (*p<0.05, **p<0.01, control MO vs. <i>lin-28a</i> MO, control MO vs. <i>lin-28b</i> MO, two-tailed test, n = 4, mean ± SEM). D. Semiquantitative RT-PCR analysis of <i>lin-41</i> gene expression in control MO-, lin-28a MO- and lin-28b MO-injected embryos. Total RNA was isolated from <i>lin-28a</i> MO-, <i>lin-28b</i> MO- or control MO-injected embryos at 24 hpf and analyzed by using specific primers for <i>lin-41.</i> The expression of <i>lin-41</i> was decreased in both <i>lin-28a</i> MO- and <i>lin-28b</i> MO-injected embryos compared with control MO-injected embryos. E. Control, <i>lin-28a</i> and <i>lin-28b</i> MO-injected embryos labeled by <i>in situ</i> hybridization with <i>lin-41</i> probe. Expression of <i>lin-41</i> was repressed in CNS of both <i>lin-28a</i> and <i>lin-28b</i> morphant embryos at 24 hpf.</p
Temporal analyses of <i>lin-14</i> mRNA, protein, and <i>lin-4</i> miRNA levels in wild-type and <i>lin-14(n355n679)</i> mutant animals.
No full text<p>(A, C) Quantification of <i>lin-14</i> mRNA, LIN-14 protein and <i>lin-4</i> miRNA from early L1 (0 hours post-feeding) to early L2 (24 hours post-feeding) in wild-type (A) and <i>lin-14(n355n679)</i> mutant animals (C). All results are shown relative to the level at the 0 hour time point. Error bars represent SEM for two independent experiments. (B, D) Representative immunoblots showing the abundance of LIN-14 protein in wild-type (B) and <i>lin-14(n355n679)</i> mutant animals (D) over 24 hours of development. Actin serves as a control for the normalization of LIN-14.</p
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