1,721,005 research outputs found
LIN, YU-LING
No full text茭白筍可食用的筍莖是黑穗菌於菰菌中寄生所形成的菌攲瘦，此種黑穗菌為一種有益
的食用真菌，茭白筍為本省重要之夏季蔬菜。1981年林及張由解剖學進行茭白筍與非
感染株莖組織之比較觀察，發現茭白組織由於菰黑穗菌菌絲之侵入，將菌絲週圍之細
胞擠壓形成菌腔，由於細胞數目及大小的變化，及菌腔週圍之組織被誘導產生許多異
常形成層，因而認為有某種植物生長素，在此膨大過程直接或間接地參與作用；此外
1982年呂及張利用掃描電子顯微鏡觀察，此菌在茭白筍組織中增生情形，情形亦同。
基於解剖學的觀點，本論文乃著重於探討可以刺激細胞分裂的細胞分裂素，利用高效
液用色層分析儀及生物分析方法，檢定茭白筍中細胞分裂素的量及生物活性。結果發
現游離態的細胞分裂素量甚少或不存在，而鍵結的細胞分裂素其量感染植株較非感染
株高，並且這檢定出的細胞分裂素是具有生物活性的，同時在黑穗菌上亦有發現鍵結
態細胞分裂素
Identification and molecular characterization of dPTPMEG-interacting proteins
No full text蛋白質酪氨酸磷酸酶 (Protein tyrosine kinases；PTKs)與蛋白質酪氨酸去磷酸酶 (protein tyrosine phosphatases；PTPs)相互調節酪氨酸磷酸化與去磷酸化作用，並且在細胞週期、細胞分化與細胞之移動中扮演重要的角色。而典型蛋白質酪氨酸去磷酸酶中又分為受器型 (receptor-like PTPs；RPTPs) 與非受器型 (non-transmembrane PTPs；NTPTPs) 兩種，雖然在果蠅研究中已確定許多受器型酪氨酸去磷酸酶在神經發育之功能，但多數的非受器型酪氨酸去磷酸酶之功能仍不清楚。在本論文中我將探討一個非受器型的酪氨酸去磷酸酶－dPTPMEG。根據先前研究結果已知dPTPMEG對果蠅腦中蕈狀體(mushroombody)之軸突伸展(axon projection)的維持有關。我主要利用酵母菌雙雜交系統 (yeast two-hybrid)之方法篩選出在果蠅胚胎基因庫(Drosophila embryonic cDNA library)中與dPTPMEG有交互作用的基因。經過進一步實驗確認後從中挑選出兩個可能與dPTPMEG交互作用之基因做後續研究。我利用GST 融合蛋白沉澱法(GST pull-down assays)與共同免疫沉澱法 (co-immunoprecitipation) 更進一步證實dPTPMEG與此兩蛋白間之交互作用，此外也利用果蠅的翅膀觀察其遺傳性狀交互作用。雖然在本篇論文中對dPTPMEG及其交互作用蛋白之關係有了初步的研究成果，這些蛋白之間的交互作用在果蠅發育上扮演的角色則需要更進一步的實驗研究。Protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) reciprocally regulate protein tyrosine phosphorylation, and is involved in various cellular processes, including cell cycle, proliferation, differentiation and cell movement. Classical PTPs can be classified into receptor-like PTPs (RPTPs) and non-transmembrane PTPs (NTPTPs). Although many Drosophila RPTPs have been found to act during neurogenesis, the roles of NTPTPs in development remain unclear. In this study, we focused on the dPTPMEG, one of the Drosophila classical non-transmembrane PTPs (NTPTPs), which has been reported to play a role in the maintenance of axon projection of Drosophila mushroom body. To further explore the function of dPTPMEG, the yeast two-hybrid screen was performed to isolate dPTPMEG-interacting proteins from Drosophila embryonic cDNA library. Two proteins have been identified that can potentially interact with dPTPMEG. The interaction between dPTPMEG and these two proteins was further confirmed by in vitro GST pull-down assays and in vivo co-immunoprecipitation experiments. We further investigated their genetic interactions in Drosophila developing wings. The developmental roles of these interactions will be further investigated
The Optimal Pricing Strategy of Manufacturer by Considering Distributor Providing Repair Service
No full text綠色供應鏈議題近年來受到關注，隨著科技發展日新月異，產品技術快速變遷下帶來生活型態的改變，其便利與科技化隨之而來的是對環境造成的衝擊與破壞，也因為工業發展的環境汙染問題，綠色供應鏈受到廣泛的討論。本研究以綠色供應鏈運作模式為背景，納入製造商之下游通路商與供應鏈最終端的顧客市場，探討綠色供應鏈的製造商定價策略。
某些特定產業中，從市場上將產品回收之後，通路商可以將回收品翻修後直接銷售至市場，因此將上述之產品與通路商在供應鏈角色納入探討，站在製造商的角度，考量通路商同時經銷與生產一評價不同之競爭商品下，製造商面對下游具回收與生產兩個功能的通路商，以兩階段賽局模型架構下求解出最佳定價與生產決策，本研究將提供決策建議予製造商，製造商利潤最大之下為目標，以模型解釋應如何訂定正、逆向物流之產品價格最佳決策。
研究結果顯示，製造商考量通路商決策下的最佳定價策略，受到評價、回收率、市場大小、新品和再製成本比值以及維修成本影響，因此製造商應考慮這些條件下，調整或回應與之相對的決策；其中，製造商面對維修品在市場上的評價越高則利潤越低，製造商以達到利潤最大的目標下應將回收品買回價提高；回收率越低或產品市場過小，對製造商利潤下降，最後本研究依據研究結果針對製造商於逆物流模式中之定價策略提出實務建議與管理意涵。As green supply chains (GSCs) have setting significant attention and concern, this study investigates the optimal pricing policy of a manufacturer in the green supply chain. From the perspective of a manufacturer, a distributor collect used products from customers, and it can repair the recycled products and sell to the market or sell them to the manufacturer for remanufacturing. In addition, the distributor sells the usable products purchased from the manufacturer and repaired products to the customers at the same time. Hence, this study establishes a two-stage game model, considering the interaction between a manufacturer and a distributor within the context of Bertrand duopoly and price competition to find the optimal pricing policy of the manufacturer. Next, a sensitivity analysis is conducted to discuss how different valuations for a repaired product, collection rate, market size, ratio of new product cost to remanufactured cost and repairing cost influence the manufacturer profit and distributor profit. The results show that (1) When the valuation for a repair product is higher, the manufacturer should increase the purchase price of collected products (2) The manufacturer should collaborate with the distributor to increase the collection rate to increase its profit (3) The manufacturer should choose product under the condition of low valuation for a repair product, low new product cost, and high repairing cost to increase its profit
sj-pdf-1-jhc-10.1369_00221554221116936 – Supplemental material for Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy
No full textSupplemental material, sj-pdf-1-jhc-10.1369_00221554221116936 for Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy by Yu-Ching Peng, Yu-Chieh Lin, Yu-Ling Hung, Chien-Chung Fu, Margaret Dah-Tsyr Chang, Yen-Yin Lin and Teh-Ying Chou in Journal of Histochemistry & Cytochemistry</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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