5,603 research outputs found
OPTIMUM MATERIAL COMPOSITION FOR CONTACTING INTERFACE IN TESTING OF LEAD-FREE DEVICE
No full textMaster'sMASTER OF SCIENCE IN ADVANCED MATERIALS FOR MICRO- & NANO- SYSTEMSDissertation Advisor: 1. Assoc. Prof. Wong Chee Cheong, SMA Fellow, NTU STATS ChipPAC Ltd Project Supervisors: 1. Mel Goodson, Test R&D Senior Manager 2. Lim Kok Hwa, Test R&D Senior Engineer 3. Sim Yeow Teck, Test R&D Senor Enginee
Analysis of Lim1 LIM domains in mouse development
No full textTranscriptional regulation is fundamental for the precise development of all organisms. Through tight regulation, necessary genes are activated at proper spatial and temporal patterns, while unnecessary genes are repressed. A large family of regulator proteins that have been demonstrated to be involved in various developmental processes by activation and repression of target genes is the homeodomain family of proteins. To date, the function of many of these homeoproteins has been elucidated in diverse species. However, the molecular mechanism underlying the function of these proteins has not been fully understood. In this study, the molecular mechanism of the function of a LIM-homeoprotein, Lim1, was examined. In addition to the homeodomain, Lim1 contains two LIM domains that are highly conserved among species. This high conservation along with data from in vitro studies on Xenopus Lim1 suggests that the LIM domains might be important for the function of Lim1 as a transcriptional regulator. Here, the functional importance of the LIM domains of Lim1 was determined by using a novel gene-targeting strategy in mouse embryonic stem (ES) cells. A cre-loxP system was used in conjunction with the unique genomic organization of Lim1 to obtain four types of mutant ES cell lines that would allow for the in vivo analysis of the function of both the LIM domains of Lim1 together and also singularly. These four mutant Lim1 alleles either contained base-pair changes at the LIM encoding exons that alters zinc-binding amino acids of the LIM domains or contained only exogenous loxP sequences in the first intron of Lim1, which serves as the control allele. These mutations in the LIM domains would presumably abolish the zinc-finger tertiary structure of the domain and thus render the domain non-functional. Mice carrying mutations at both the LIM domains of Lim1, L1L2, die around E10 without anterior head structures anterior to rhombomere 3, identical in phenotype to the Lim1 null mutants in spite of the presence of mutant Lim1 RNA. This result demonstrates that the integrity of both the LIM domains are essential for the function of Lim1. This is further supported by the phenotype of mice carrying mutation at only the second LIM domain of Lim1, L2. The L2 mice although still carrying one intact Lim1 LIM domain, also die in utero. The L2 mice die at varying times, from around E8 to E10 with anterior defects in addition to other axial defects which have yet to be fully characterized. The results of this study so far demonstrates that the integrity of both LIM domains are required for the function of Lim1
Request strategies in email communication in a private institution / Marchie Lim Pin Sim
No full textThis paper investigates the ways in which staff members in the workplace of a private
institution in Malaysia, realise requests in their email communications with special
reference to politeness strategies as described by Brown and Levinson (1987)
complemented by Spencer-Oatey’s (2008) rapport management framework. It provides
a pragmatic analysis of the strategies of requests speech act and politeness phenomenon
in the production of request speech acts in the emails by staff members. It aims to
identify the politeness strategies employed with regards to face and rapport management
by staff members of different professional status when making requests in their email
communication with staff members of different positions of power. It explores the
lexical choices used to indicate politeness in the request emails and investigates the
politeness markers used in greetings, closings and the requests phrases. It seeks to find
out if there is a difference in the way they construct their sentences or whether the same
structures are used when communicating with people of different positions in an attempt
to analyse the correlation between social distance and the politeness strategies employed
and the institutional social norms for rapport management. It also seeks to establish if
recipients accommodate to politeness elements in their replies. The paper is based on
the analysis of fifty request emails written by four staff members to their subordinates,
peers and superior and the responses to the requests. The study is descriptive in nature
and frequencies and qualitative analysis are used. The findings of the study revealed
that staff members use similar forms of structural and verbal politeness forms in
opening, closing and requests phrases of their emails. In their requests, they tend to use
negative politeness strategies, especially with superiors or peers and direct strategies
with subordinates in their requests, mitigated with politeness markers. There is a
tendency to use hedges in the negative politeness strategies. It was also found that
recipients accommodate politeness in their reply emails. These negative politeness strategies and mitigators are also found to function as
linguistic devices to build rapport among the institution’s staff members
SIM-Pic: Orizzonti
No full textCon questo articolo si dà il via alla rubrica SIM-Pic: in ogni numero della rivista Scuola Italiana Moderna viene inserita una raccolta di foto tematiche, la maggior parte scattate ad hoc, e rese disponibili anche on line per permetterne la fruizione da ogni tipologia di device (proiettore, LIM, tablet) che sia a disposizione in
classe o, se si desidera, anche per
la stampa. Negli articoli di SIM-Pic di tutta l'annata 2014/15 verrà fornito qualche piccolo spunto per utilizzare tali immagini a supporto
e integrazione di attività didattiche
Fig. 1 in Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura
No full textFig. 1. Structures of compounds 1–11, 14, and 20–21.Published as part of Tang, Sin-Yee, Tan, Chun-Hoe, Sim, Kae-Shin, Yong, Kien-Thai, Lim, Kuan-Hon, Low, Yun-Yee & Lim, Siew-Huah, 2023, Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura, pp. 113587 in Phytochemistry (113587) 208 on page 2, DOI: 10.1016/j.phytochem.2023.113587, http://zenodo.org/record/816067
Fig. 5 in Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura
No full textFig. 5. Proposed biosynthetic pathway to 1.Published as part of Tang, Sin-Yee, Tan, Chun-Hoe, Sim, Kae-Shin, Yong, Kien-Thai, Lim, Kuan-Hon, Low, Yun-Yee & Lim, Siew-Huah, 2023, Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura, pp. 113587 in Phytochemistry (113587) 208 on page 8, DOI: 10.1016/j.phytochem.2023.113587, http://zenodo.org/record/816067
Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura
No full textTang, Sin-Yee, Tan, Chun-Hoe, Sim, Kae-Shin, Yong, Kien-Thai, Lim, Kuan-Hon, Low, Yun-Yee, Lim, Siew-Huah (2023): Polyneurines A H, iboga alkaloids from Tabernaemontana polyneura. Phytochemistry (113587) 208: 113587, DOI: 10.1016/j.phytochem.2023.113587, URL: http://dx.doi.org/10.1016/j.phytochem.2023.11358
An Alcohol Dehydrogenase Gene from Synechocystis sp Confers Salt Tolerance in Transgenic Tobacco
No full textSynechocystis salt-responsive gene 1 (sysr1) was engineered for expression in higher plants, and gene construction was stably incorporated into tobacco plants. We investigated the role of Sysr1 [a member of the alcohol dehydrogenase (ADH) superfamily] by examining the salt tolerance of sysr1-overexpressing (sysr1-OX) tobacco plants using quantitative real-time polymerase chain reactions, gas chromatography-mass spectrometry, and bioassays. The sysr1-OX plants exhibited considerably increased ADH activity and tolerance to salt stress conditions. Additionally, the expression levels of several stress-responsive genes were upregulated. Moreover, airborne signals from salt-stressed sysr1-OX plants triggered salinity tolerance in neighboring wild-type (WT) plants. Therefore, Sysr1 enhanced the interconversion of aldehydes to alcohols, and this occurrence might affect the quality of green leaf volatiles (GLVs) in sysr1-OX plants. Actually, the Z-3-hexenol level was approximately twofold higher in sysr1-OX plants than in WT plants within 1-2 h of wounding. Furthermore, analyses of WT plants treated with vaporized GLVs indicated that Z-3-hexenol was a stronger inducer of stress-related gene expression and salt tolerance than E-2-hexenal. The results of the study suggested that increased C-6 alcohol (Z-3-hexenol) induced the expression of resistance genes, thereby enhancing salt tolerance of transgenic plants. Our results revealed a role for ADH in salinity stress responses, and the results provided a genetic engineering strategy that could improve the salt tolerance of crops.
© 2017 Yi, Ku, Sim, Kim, Park, Lyu, So, Choi, Kim, Ahn, Kim, Park,
Jeong, Lim, Min and Liu. This is an open-access article distributed under the terms
of the Creative Commons Attribution License (CC BY). The use, distribution or
reproduction in other forums is permitted, provided the original author(s) or licensor
are credited and that the original publication in this journal is cited, in accordance
with accepted academic practice. No use, distribution or reproduction is permitted
which does not comply with these terms
sj-xlsx-2-tam-10.1177_17588359231206259 – Supplemental material for Classification of HER2-negative breast cancers by ERBB2 copy number alteration status reveals molecular differences associated with chromosome 17 gene aberrations
No full textSupplemental material, sj-xlsx-2-tam-10.1177_17588359231206259 for Classification of HER2-negative breast cancers by ERBB2 copy number alteration status reveals molecular differences associated with chromosome 17 gene aberrations by Jui Wan Loh, Abner Herbert Lim, Jason Yongsheng Chan and Yoon-Sim Yap in Therapeutic Advances in Medical Oncology</p
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