1,721,049 research outputs found
DNA-Encoded Combinatorial Library of Macrocyclic Peptoids
No full textWe report the design and synthesis of a DNA-encoded one-bead one-compound library of cyclic peptoids composed of more than 11 million molecules. We show that affinity-based screening of this large library can identify cyclic peptoid ligands for a target protein. In this work, we developed a simple method for amplifying the PCR product from DNA tags on a single bead, thereby enabling determination of the structures of hit cyclic peptoids with no need for high-throughput sequencing and complicated data analysis. We also developed a sublibrary screening strategy to minimize false positives caused by the interference of coding DNA tags before starting laborious and impractical hit confirmation. Given the simplicity and robustness of the synthesis and screening, along with the desirable features of macrocyclic peptoids including improved conformational rigidity, our method will be highly useful for discovering biologically active molecules modulating challenging targets such as protein-protein interactions that are not easily targeted by typical peptidomimetics and small-molecules.11Nsciescopu
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Air quality measurement using remote sensing and digital images processing techniques / Lim, H. S. … [et al.]
Get PDFA conventional digital camera Kodak DC 290 was used as a sensor to capture digital images of our reference targets. The digital images were separated into three bands namely red, green and blue bands for multispectral algorithm calibration. In-situ measurements of corresponding air pollution parameters were carried out at the ASMA air pollution station in Universiti Sains Malaysia campus, Penang. The selected parameter was particulate matter less than 10 micron (PMlO). We put up four different colour paper (Red, Green, Blue and Black) on the wall of a building as reference surfaces. Digital images were captured at close and far distance from the colour papers/wall of the building. These in-situ measurements were then used as the dependent variables in deriving the air quality information using the digital camera data. In fact, air quality parameters can be measured using ground instrument such as air sampler. But these instruments are quite expensive, and a limited number of the air pollutant stations are available in each area. Most countries have established a network for measuring the air quality levels. Due to the high cost and limited number of the air pollutant stations in the each area, they cannot provide a good spatial distribution of the air pollutant reading over a city. The objective of this study was to test the potential of using remote sensing and digital image processing techniques for the air quality measurements. The digital numbers of three bands were converted into irradiance. and then reflectance. The relationship between the reflectance and the corresponding air quality data was determined using regression analysis. A new algorithm was developed for detecting air pollution from the digital camera images chosen based on the highest correlation coefficient, R and lowest root mean square error, RMS for PM10. The algorithm used was based on the apparent reflectance values detected at near and far distances from the refence surface, and these in turn can be related to the concentration of the air pollutants. The coefficients of the calibrated algorithm were determined and used in estimating the air pollution level. The newly developed algorithm produced a high degree of accuracy with the correlation coefficient (R) of 0.9001 and the root-mean-square error (RMS) of 5.1008 µg/m³ for PM10. Comparison was made between the air pollution parameter and the results using different colour paper or wall of a building as a reference surface. We found that the red colour paper produced the best result when it was used as a reference surface in this study. The pollutant's concentration can be estimated accurately at a relatively much cheaper cost compared with other techniques
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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