68,205 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Epanerchodus gangwonus Mikhaljova & Lim 2002
No full textEpanerchodus gangwonus Mikhaljova & Lim, 2002 Epanerchodus gangwonus Mikhaljova & Lim, 2002: 19 –21, 20: figs 1–8. Remarks. Originally described from Gangwon-do, South Korea (Mikhaljova & Lim, 2001), this species has since never been rediscovered. Distribution. South Korea.Published as part of M, E L E N A V., Va, I K H A L J O & Lim, Kil-Young, 2006, The millipede genus Epanerchodus Attems, 1901 in the Korean Peninsula, with a description of a new species (Diplopoda, Polydesmida, Polydesmidae), pp. 45-53 in Zootaxa 1350 on page 48, DOI: 10.5281/zenodo.17451
Four and a half LIM protein 1C (FHL1C)
No full textFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
Goniozus koreanus Lim, sp. nov.
No full textGoniozus koreanus Lim, sp. nov. (Figs 17–24) Type material. Holotype, Ƥ. KOREA: CN: Mangilsa, Daesan, Daesan, Seosan, N 36 ° 56 ' 29.8 " E 126 ° 26 ' 85.1 ", Alt. 184 m, 20.v. 2006, S.W. Park leg. (SNU). Paratypes. KOREA: Seoul: Ƥ, Cheongyangri, Dongdaemun, MT, 25.vii– 1.viii. 2005, D.P. Lyu leg. (KFRI); Ƥ, ditto, 15–22.viii. 2005, D.P. Lyu leg. (KFRI); Ƥ, Mt. Surak, Sanggye, Nowon, MT, 18.vii– 24.viii. 2007, J. O. Lim leg. (SNU); Ƥ, Seoul National University campus, Daehak, Gwanak, 4.viii. 2008, J. O. Lim leg. (SNU); Ƥ, Mt. Bulam, Gongreung, Nowon, MT, 11–25.v. 2008, S.W. Park leg. (SNU). GG: Ƥ, Yongin, 21.v. 1989, S.B. Han leg. (SNU); Ƥ, Mt. Yeogi, Seodun, Gwonseon, Suwon, 16.iv. 1994, J. Y. Choi leg. (SNU); Ƥ, Mt. Cheonggae, Gwacheon, 22.ix. 2000, H. G. Kang leg. (SNU); Ƥ, Yeongjusa, Annyeong, Taean, Hwaseong, MT, 22–29.viii. 2005, Y.D. Kwon leg. (KFRI); 2 Ƥ, ditto, 5 – 2.ix. 2005, Y.D. Kwon leg. (KFRI); 2 Ƥ, ditto, 12–20.ix. 2005, Y.D. Kwon leg. (KFRI); Ƥ, Sihwado, Namyangju, MT, N 37 ° 40 ' 6 " E 127 ° 18 ' 39 ", Alt. 238 m, 27.v. 2007, S.W. Park leg. (SNU); Ƥ, Gwanak arboretum, Anyang, Manan, Anyang, MT, 26.vi– 4.vii. 2007, J. O. Lim leg. (SNU); Ƥ, ditto, MT, N 37 ° 25 ' 15.6 " E 126 ° 56 ' 44.3 ", Alt. 126 m, 18.iv– 2.v. 2008, J. O. Lim leg. (SNU); Ƥ, Suwon arboretum, Seodun, Gwonseon, Suwon, 1.vi. 2009, J. O. Lim leg. (SNU); Ƥ, Mt. Ungil, Songchon, Choam, Namyangju, MT, N 37 ° 34 ' 43.3 " E 127 ° 18 ' 37.5 ", Alt. 134 m, 18–31.iv. 2009, J. O. Lim leg. (SNU); Ƥ, ditto, MT, 1–26.v. 2009, J. O. Lim leg. (SNU); Ƥ, ditto, MT, 27.v– 10.vi. 2009, J. O. Lim leg. (SNU); Ƥ, Mt. Homyeong, Goseong, Cheongpyeong, Gapyeong, MT, N 37 ° 43 '15.0" E 127 ° 29 ' 18.9 ", Alt. 168 m, 18–31.iv. 2009, J. O. Lim leg. (SNU); 2 Ƥ, ditto, MT, 1–6.v. 2009, J. O. Lim leg. (SNU). GW: Ƥ, Jinae, Dong, Chuncheon, MT, 16–22.viii. 2005, S.J. Jang leg. (KFRI); Ƥ, ditto, MT, 31.vii– 7.viii. 2008, S.J. Jang leg. (KFRI); Ƥ, Jukheon, Gangreung, N 37 ° 46 ' 55 " E 128 ° 51 ' 35 ", Alt. 57 m, 29.v. 2009, S.W. Park leg. (SNU); Ƥ, Chundang, Cheongil, Hoengseong, N 37 ° 36 ' 36 " E 128 ° 8 ' 36 ", Alt. 249 m, 7.vi. 2009, S.W. Park leg. (SNU). CB: Ƥ, Mt. Wolak, Susan, Jecheon, MT, N 36 ° 52 ' 4 " E 128 ° 8 ' 57 ", 1.ix. 2006, J. C. Jeong leg. (SNU); Ƥ, Namdaemun, Hoenam, Boeun, N 36 ° 26 ' 27 " E 127 ° 34 ' 25 ", Alt. 104 m, 24.ix. 2009, S.W. Park leg. (SNU). CN: Ƥ, Donam, Banpo, Gongju, MT, 23–30.viii. 2005, J.H. Han leg. (KFRI); 2 Ƥ, Gahak, Songak, Dangjin, N 36 ° 55 ' 17.5 " E 126 ° 42 ' 33 ", Alt. 34 m, 19.v. 2006, S.W. Park leg. (SNU); Ƥ, Baekja, Susin, Cheonan, 6.vi. 2008, S.W. Park leg. (SNU); 2 Ƥ, Annyeong, Tancheon, Gongju, 24.v. 2009, S.W. Park leg. (SNU); Ƥ, Hwaam, Cheongra, Boryeong, 14.vi. 2009, S.W. Park leg. (SNU); Ƥ, Hanseo Univ., Daegok, Haemi, Seosan, MT, N 36 ° 41 ' 30 " E 126 ° 34 ' 50 ", 11.vi– 8.vii. 2009, J.W. Lee leg. (YNU); Ƥ, Masan, Seocheon, 12.vi. 2010, S.W. Park leg. (SNU). Daejeon: 3 Ƥ, Wolpyeong, Seo, MT, 20.vi– 10.vii. 2008, J.W. Lee leg. (YNU). JB: Ƥ, Majeong, Bug, Jeongeub, MT, 19–26.vii. 2005, J.W. Park leg. (KFRI); Ƥ, ditto, 2–9.viii. 2005, J.W. Park leg. (KFRI); Ƥ, ditto, 30.viii– 6.ix. 2005, J.W. Park leg. (KFRI); Ƥ, Majeong, Bug, Jeongeub, MT, 19.iv– 8.v. 2007, J.W. Park leg. (KFRI); [JN] Ƥ, Pungsan, Dado, Naju, MT, 25.vii– 8.viii. 2005, S.B. Yu leg. (KFRI); Ƥ, ditto, 9–30.ix. 2005, S.B. Yu leg. (KFRI); 2 Ƥ, Pungsan, Dado, Naju, MT, 27.iv– 17.v. 2007, S.B. Yu leg. (KFRI); 2 Ƥ, ditto, 17.v– 7.vi. 2007, S.B. Yu leg. (KFRI); Ƥ, Mt. Naejang, Ssangung, Bukha, Jangseong, MT, N 35 ° 25 ' 31.6 " E 126 ° 51 ' 46.9 ", 13.v. 2007, J.W. Lee leg. (YNU); 2 Ƥ, Pungsan, Dado, Naju, MT, 26.v– 2.vi. 2008, S.B. Yu leg. (KFRI); Ƥ, Mt. Naejang, Sinseong, Bukha, Jangseong, N 35 ° 27 ' 17.9 " E 126 ° 50 ' 38.8 ", Alt. 161 m, 3.vii. 2009, J. O. Lim leg. (SNU). GB: Ƥ, Yeungnam Univ., Dae, Gyeongsan, MT, 30.iv– 7.v. 2007, J.W. Lee leg. (YNU); Ƥ, Namsa, Hyeongok, Kyeongju, MT, 30.vi– 14.vii. 2005, J.T. Mun leg. (KFRI); 2 Ƥ, Namsan, Gakbuk, Cheongdo, MT, N 35 ° 41 ' E 128 ° 35 ', 9–19.viii. 2007, J.W. Lee leg. (YNU); Ƥ, ditto, 15.x– 4.xi. 2007, J.W. Lee leg. (YNU); Ƥ, Yeongnam Univ., Dae, Gyeongsan, MT, 30.iv– 7.v. 2007, J.W. Lee leg. (YNU); Ƥ, ditto, MT, N 35 ° 58 ' E 128 ° 47 ', 12–21.vii. 2007, J.W. Lee leg. (YNU); Ƥ, Namsan, Gakbuk, Cheongdo, N 35 ° 41 ' E 128 ° 35 ' 23 ", 5.x– 2.xi. 2008, J. O. Lim leg. (SNU); Ƥ, Mt. Unmun, Cheongdo, MT, N 35 ° 38 ' 45 " E 128 ° 57 ' 33 ", 23.v. 2008, J.W. Lee leg. (YNU); Ƥ, ditto, MT, N 35 ° 38 ' 19 " E 128 ° 57 ' 40 ", 30.v– 16.vi. 2009, C. J. Kim leg. (YNU); Ƥ, Sangju campus, Gyeongbuk Univ., Gajang, Sangju, MT, 28.v– 4.vi. 2009, S.W. Park leg. (SNU). GN: Ƥ, Dapcheon, Ibanseong, Jinju, MT, 1–9.viii. 2005, B.G. Ahn leg. (KFRI). Busan: Ƥ, Daemadeung, Nakdonghagu, Myeongji, Gangseo, 22.viii. 2006, T. H. Kim leg. (SNU). JJ: Ƥ, Donggye, Jeju, MT, 27.vi– 18.vii. 2007, C. H. Shin leg. (KFRI). Diagnosis. This species is mostly similar to Goniozus japonicus Ashmead, 1904 by having mandible yellow; by fore wing without areolet; by flagellomere 3–5 longer than wide respectively; by propodeal disc with complete transverse carina; by ratio of head and propodeal disc. However, this species can be distinguished from G. japonicus by short antennal segments, by pedicel to flagellomere 2 less than 1.5 × as long as wide, by flagellomere 11 1.5 × as long as wide (long antennal segments, pedicel to flagellomere 2 longer than 2.0 × as long as wide, flagellomere 11 2.0 × as long as wide in G. japonicus); by median and submedian cell of fore wing with relatively denser hairs (very sparse hairs in G. japonicus). Description. FEMALE (holotype). Body length 4.1 mm. LFW 2.5 mm. Color. Head: mandible yellow, antenna yellow, from flagellomeres 6–11 pale castaenous. Mesosoma black; fore wing subhyaline, veins pale castaenous; legs yellow except coxa and femora dark castaenous, tarsal claw dark castaenous. Metasoma black except distal margin of terga 4–7 pale castaenous. Head (Figs 18–20): 1.0 × as long as wide, coriaceous; lateral margin convex, posterior margin straight, postero-lateral corner forming round angle in dorsal view; lateral surface smooth and polished. Mandible with four acute teeth. Clypeus well-developed, frontal angle right; fronto-clypeal median longitudinal carina developed, exceeding antennal socket. First antennal segment in ratio of 2.3: 1.0: 1.0: 1.1: 1.2 in length; from scape to flagellomere 3 and 11 2.0, 1.3, 1.2, 1.2, 1.3 and 1.6 × as long as wide, respectively. Frons and vertex coriaceous with sub-erect hairs and sparse moderate punctures, aparted from each other 2.0–3.0 × as wide as their maximum diameter. WF 1.1 × LE, WF 0.6 × WH. Compound eye 0.37 mm long without hairs. LE 1.8 × OOL, WF 1.7 × WOT. Frontal angle of ocellar triangle obtuse, POL 2.1 × AOL, OOL 0.8 × WOT. Vertex coriaceous without conspicuous long hairs. Mesosoma (Figs 21–23): Pronotum coriaceous, 0.4 × as long as wide with sparse hairs, antero-lateral corner obtuse. Mesoscutum coriaceous; notauli absent; parapsidal furrows thin and anteriorly divergent. Scutellum polish and coriaceous with sparse small punctures; scutellar pit elliptical, oblique and connected by 3.9 × as wide as their maximum diameter. Propodeal disc 0.6 × as long as wide, lateral and transverse carina complete; medial basal triangle smooth and polished, extending mid-length of disc, connected to transverse carina with thin longitudinal carina in areolate surface. Disc areolate-rugose; declivity coriaceous with complete marginal carina; lateral surface coriaceous. Fore wing without closed areolate; median and submedian cell with two rows of hairs; radial vein curved outward at apex with obtuse angle; pterostigma 0.29 mm long; metacarpo absent. Metasoma (Fig. 24): Tergite 1 smooth and polished without fine puncture and microreticulation. Terga 2–4 smooth and micoreticulation on anterior half with some hairs on dorso-lateral surface. Terga 5–7 microreticulate with sparse hairs on distal surface. MALE. Unknown. Distribution. Korea (Busan, CB, CN, Daejeon, GB, GG, GN, GW, JB, JJ, JN, Seoul).Published as part of Lim, Jongok & Lee, Seunghwan, 2012, Review of Goniozus Förster, 1856 (Hymenoptera: Bethylidae) of Korea, with descriptions of two new species, pp. 43-57 in Zootaxa 3414 on pages 49-51, DOI: 10.5281/zenodo.21079
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Do insectivorous bird communities decline on land-bridge forest islands in Peninsular Malaysia?
No full textTo assess the impact of habitat fragmentation on tropical avian communities, we sampled lowland forest birds on six land-bridge islands and two mainland forest sites in Lake Kenyir, Peninsular Malaysia using timed point counts, hypothesizing that insectivorous birds are the worst affected guild. We used an information-theoretic approach to evaluate the effects of area, isolation, primary dietary guild (omnivore, frugivore and insectivore) and their interactions in predicting species richness, abundance and diversity. Our analysis showed that a model that considered the effects of area, dietary guild and their interaction best explained observed patterns of species richness. But amodel considering both area and dietary guild best explained the variation in abundance. Notably, insectivorous birds were singled out as the dietary guild most sensitive to fragmentation, followed by frugivorous and omnivorous birds and hence provide support for our hypothesis. Assemblages of insectivorous birds were clearly depauperate on anthropogenic forest islands in Lake Kenyir and are consistent with forest fragmentation studies in the Neotropics. Given their specialized foraging ecology and diversity, conservation of intact communities of insectivorous bird guilds in Malaysia will be critical for maintaining predator–prey interactions in lowland tropical forests.Ding Li Yong, Lan Qie, Navjot S. Sodhi, Lian Pin Koh, Kelvin S.-H. Peh, Tien Ming Lee, Haw Chuan Lim and Susan L.-H. Li
Reconstruction of small interface changes of an inclusion from modal measurements II: The elastic case
No full textIn order to reconstruct small changes in the interface of an elastic inclusion from modal measurements, we rigorously derive an asymptotic formula which is in some sense dual to the leading-order term in the asymptotic expansion of the perturbations in the eigenvalues due to interface changes of the inclusion. Based on this (dual) formula we propose an algorithm to reconstruct the interface perturbation. We also consider an optimal way of representing the interface change and the reconstruction problem using incomplete data. A discussion on resolution is included. Proposed algorithms are implemented numerically to show their viability. (C) 2010 Elsevier Masson SAS. All rights reserved
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Physical activity among hospitalised older people:: insights from upper and lower limb accelerometry
No full textBackground Few studies have explored the activity levels of hospitalised older people and the intra-daily activity patterns in this group have not been described.Aims To describe the quantity and daily pattern of physical activity among hospitalised older people using two accelerometers: the ankle-worn StepWatch Activity Monitor (SAM), and the wrist-worn GENEActiv.Methods This cross-sectional observational study was conducted on the acute medical wards for older people in one UK hospital. Inclusion criteria: participants aged ≥ 70 years, and able to mobilise prior to admission. Participants wore bothdevices for up to seven consecutive days, or until hospital discharge, whichever was sooner. Intra-daily activity levels were analysed hourly over each 24 h period.Results 38 participants (mean age 87.8 years, SD 4.8) had their activity levels measured using both devices. The SAM median daily step count was 600 (IQR 240–1427). Intra-daily activity analysis showed two peak periods of ambulatory activity between 9 am–11 am and 6 pm–7 pm. With physical activity defined as ≥ 12 milli-g (GENEActiv), the median time spent above this cut-off point was 4.2 h. 62% of this activity time was only sustained for 1–5 min. Acceptability of both devices was high overall, but the wrist-worn device (96%) was more acceptable to patients than the ankle-worn device (83%).Conclusion Activity levels of these hospitalised older people were very low. Most physical activity was sustained over short periods. The intra-daily pattern of activity is an interesting finding which can help clinicians implement time-specific interventions to address the important issue of sedentary behaviour
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