73 research outputs found
Self-quenching of carboxyfluorescein fluorescence: Uses in studying liposome stability and liposome-cell interaction
No full textJohn N. Weinstein, Evelyn Ralston, Lee D. Leserman, Richard D. Klausner, Paul Dragsten, Pierre Henkart, and Robert Blumenthal IV. Applications of Fluorescence Self-Quenching(FSQ)……………………….195 A. Release from Liposomes In Vitro…………………………………195.SCOPUS: ch.bSCOPUS: ch.binfo:eu-repo/semantics/publishe
Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes
No full textEncapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides. This approach circumvents problems of extracellular degradation by nucleases and poor membrane permeability which free phosphodiester oligonucleotides are subject to, but leaves unresolved the inefficiency of encapsulation of oligonucleotides in liposomes. We have coupled oligonucleotides to cholesterol via a reversible disulfide bond. This modification of oligonucleotides improved their association with immunoliposomes by a factor of about 10 in comparison to unmodified oligonucleotides. The presence of cholesteryl-modified oligonucleotides incorporated in the bilayer of liposomes did not interfere with the coupling of the targeting protein to the liposome surface. Free or cholesterol coupled oligonucleotides associated with liposomes and directed against the tat gene of HIV-1 were tested for inhibition of HIV-1 proliferation in acutely infected cells. We demonstrate that the cholesteryl-modified as well as unmodified oligonucleotides acquire the target specificity of the antibody on the liposome. Their antiviral activity when delivered into cells is sequence-specific. The activity of these modified or unmodified oligonucleotides to inhibit the replication of HIV was the same on an equimolar basis (EC50 around 0.1 μM). Cholesterol coupled oligonucleotides thus offer increased liposome association without loss of antiviral activity
EMBO workshop on immunology and metabolism.
No full textInternational audienceRecent findings have identified roles for the immune system in what were previously considered to be prototypic metabolic diseases, and metabolic control has emerged as an important determinant of immune function. Giovanna Chimini, Lee Leserman, Diane Mathis and Philippe Naquet organized the EMBO Workshop on Immunology & Metabolism, which took place in January 2011 at the Centre d'Immunologie de Marseille-Luminy (CIML) in Marseille, France. The meeting brought together approximately 100 scientists to discuss interactions between metabolism and inflammation
Endocytosis of Cell Surface Molecules and Intracellular Delivery of Materials Encapsulated in Targeted Liposomes
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