108 research outputs found
Inae Sharon Lee, Violin; Junsoo Park, Violin
No full textSonata for Violin and Piano in D minor, Op. 75 / Camille Saint Saens; Por Una Cabeza / Carlos Garde
FIGURE 4. Anurida decipiens Yosii, 1966 in Cave species of the genus Anurida (Collembola: Neanuridae) from Korea, with the description of new species
No full textFIGURE 4. Anurida decipiens Yosii, 1966: A, Labium; B, Labrum; C, Maxillar lamellae; D, Mandible; E, Claw; F, Sensilla with Ant III & IV.Published as part of Lee, Inae & Park, Kyung-Hwa, 2016, Cave species of the genus Anurida (Collembola: Neanuridae) from Korea, with the description of new species, pp. 589-599 in Zootaxa 4184 (3) on page 595, DOI: 10.11646/zootaxa.4184.3.12, http://zenodo.org/record/16510
Anurida
No full textKey to the species of Korean Anurida 1 Ocelli absent........................................................................................ 2 - Ocelli present...................................................................... A. tullbergi Schött, 1891 2 Head with dorsal macrochaetae.......................................................................... 3 - Head without dorsal macrochaetae.................................................... A. decipiens Yosii, 1966 3 Abd IV p1 as macrochaetae...................................................... A. plurichaetotica Yosii, 1966 - Abd IV p1 as microchaetae........................................................ Anurida troglodyta sp. nov.Published as part of Lee, Inae & Park, Kyung-Hwa, 2016, Cave species of the genus Anurida (Collembola: Neanuridae) from Korea, with the description of new species, pp. 589-599 in Zootaxa 4184 (3) on page 590, DOI: 10.11646/zootaxa.4184.3.12, http://zenodo.org/record/16510
Complete mitochondrial genome of the Korean endemic springtail Homidia koreana Lee & Lee, 1981 (Collembola: Entomobryidae)
No full textThe Korean endemic springtail Homidia koreana Lee & Lee, is popular in the leaf litter of various forests of South Korea, probably widely distributed throughout the Korean Peninsula. The complete mitochondrial genome of H. koreana was sequenced, assembled, and annotated. The mitochondrial genome of H. koreana consists of a circular DNA molecule of 14,846 bp, with 68.4% AT content. It comprises 13 protein-coding genes (PCGs), 22 transfer (tRNA) genes, and 2 ribosomal RNA (rRNA) genes. The molecular phylogenetic relationships estimated using MrBayes 3.2 revealed that H. koreana was closely related to Homidia socia Denis, 1929, both of which belong to the genus Homidia
Anurida troglodyta Lee & Park, 2016, sp. nov.
No full textAnurida troglodyta sp. nov. Figs 1–2 Diagnosis. Colour in alcohol creamy white. Body shape slender, elongate. Ocelli absent. Ant IV with 16–17 blunt sensilla and trilobed apical bulb. PAO morel-like with more than 100 vesicles. Mandibles with 5 teeth: 3 apical ones in oblique transverse row, large thin basal one and intermediary tooth. Maxilla with strong, toothed capitulum and 3 serrated lamellae. Chaetom plurichaetotic and clearly differentiated: head with 2 mesochaetae and 4 macrochaetae, Th II–Abd IV without dorsal but with well-developed lateral macrochaetae. Abd IV, ‘sensory chaetae’ s = p4. Abd VI truncated. Type locality. Hoeokgul (cave) (N37°16´25.8˝ E128°05´39.8˝, 492 m above sea level, 50m long showing vertical morphology), located in Chiaksan (mountain), Seongnam-ri, Sillim-myeon, Wonju-si, Gangwon-do Province, Korea. Type material. Holotype (male) on slide, 4-vi-2005; 6 paratypes (3 females & 3 males) on slides, same data as holotype; 2 paratypes on SEM stub and 6 paratypes in ethyl alcohol, same locality as holotype; 27.vii.2000, 4.vi.2005, 3.viii.2005; hand collected in dark zone (30m from entrance) of limestone cave by Choi HG leg. Etymology. The name “ troglodyta ” reflects the species occurrence in deep inside cave. Korean name of the species: Dong-gul-minteok-munui-tok-to-gi. Description. Body length 3.0– 3.8 mm. Colour in alcohol creamy-white. Body shape slender, elongate (Figs 1 A, 2A, 2D). Paratergites on thorax and abdomen lobed laterally, Abd VI truncated. Body integument with uniform, coarse granulation. Ocelli absent. Ant IV as in Fig. 1 B, with 16–17 curved sensilla; external ms and subapical organite present; trilobed apical papilla present subterminally. Ant III and IV fused dorsally. Sensory organ of Ant III consisting of two sensory pegs, two long, widely separated outer sensilla, and a small ventral ms. Ant II with 12 chaetae. Ant I with 7 chaetae. PAO morel-like with more than 100 vesicles in a bunch (Fig. 2 C); each vesicle with lots of perforations on SEM photo. Mandibles with 5 teeth: 3 apical ones in oblique transverse row, large basal one and strong intermediary tooth (Fig. 1 C). Maxillary capitulum with 4 teeth and three serrated lamellae: lam.2 longer than capitulum (Figs 1 D, 2B). Labral formula interpreted as 4/2334. Labium with 10 chaetae on each side: apical part with 4 chaetae and 2 small, peg-like sensilla (as in Fig. 4 A). Female genital plate with 18–26 microchaetae; male genital plate with about 34–36 microchaetae. Unguis with inner tooth. Furcal area on Abd IV with a pair of small tubercles with 1 chaeta near the basis. Chaetotaxy. Dorsal chaetotaxy moderately plurichaetotic (Fig. 1 A), especially on head, thorax and laterally on pleura: the position and numbers of some chaetae varied; macro- and microchaetae well differentiated, head dorsally with 2 mesochaetae and 4 macrochaetae, without dorsal macrochaetae on Th II–Abd IV. Macro- and microchaetae slightly serrated, sensilla smooth. Lateral macrochaetae strongly developed: head with 4; Th I–Th III with 1,1–1,1–1; Abd I–VI with 2,2,2,4,5,6 on each half, respectively. Thoracic tergum I dorsally with 7–9 +7–9 microchaetae and 1 + 1 mesochaetae; thoracic terga II–III with two chaetal clusters each (dorso-anterior and dorsoposterior): thoracic tergum II dorsally with 9–10+9–10 chaetae in DA and 5–6+ 5–6 in DP, ‘sensory chaetae’ s = p3 and m6; thoracic tergum III dorsally with 8+8 chaetae in DA and 5–6+ 5–6 in DP, ‘sensory chaetae’ s = p3 and m6; abdominal terga I–III with 7+7 chaetae between sensory chaetae s (p4); abdominal tergum IV dorsally with 10+10 chaetae, ‘sensory chaetae’ s = p4. Abdominal tergum V, ‘sensory chaetae’ s = p3. Formula of ‘sensory chaetae’ s per half tergum: 022/11111. Lateral microsensilla (ms) present on thoracic tergum II, absent on thoracic tergum III. Tibiotarsi I, II and III with 19, 19 and 18 chaetae, respectively. Ventral tube with 8–9+8–9 chaetae. Biology. It inhabits dark zone of vertical limestone cave. The cave atmosphere has a relatively constant temperature throughout the year: air temperature ranges from 2 to 7.5? and humidity ranges from 81 to 90 %. Discussion. A. troglodyta sp. nov. has a morel-like PAO, but has no occelli. Eleven previously described congenera share these characteristics (Jordana et al. 2012). The new species has about 16–17 sensilla on Ant IV, which is more than typical of most species of Anurida, and that can be considered as a troglobiontic character together with big size, multiplication of PAO lobes and specific chaetotaxy with long lateral setae. A. troglodyta sp. nov. is rather similar to A. plurichaetotica Yosii, 1966 described from a cave of Korea, and A. okamotoi Yosii, 1970 and A. irieti Yosii, 1970 described from caves of Japan, in the presence of differentiated macrochaetae on dorsal area of a head. However this new species clearly differs by size of p1 chaeta on abdominal terga IV or V: small on Abd IV and V in A. troglodyta sp. nov., large on Abd IV and (or) V in three other species. Apart from this, A. troglodyta sp. nov. can easily be identified by characteristic PAO. Other differences between A. troglodyta sp. nov. and similar species are summarized in Table 1. Distribution. Korea.Published as part of Lee, Inae & Park, Kyung-Hwa, 2016, Cave species of the genus Anurida (Collembola: Neanuridae) from Korea, with the description of new species, pp. 589-599 in Zootaxa 4184 (3) on pages 590-593, DOI: 10.11646/zootaxa.4184.3.12, http://zenodo.org/record/16510
sj-pdf-1-ijq-10.1177_16094069211046429 – Supplemental Material for Lived Experience of Participants of a Korean Medicine-Based Postpartum Program: A Protocol for a Qualitative Research Study
No full textSupplemental Material, sj-pdf-1-ijq-10.1177_16094069211046429 for Lived Experience of Participants of a Korean Medicine-Based Postpartum Program: A Protocol for a Qualitative Research Study by Inae Youn, Han-Song Park, Do-Eun Lee, Hyo-Weon Suh and Joohee Seo in International Journal of Qualitative Methods</p
EHMC의 생애주기 노출에 따른 일본산 송사리의 생식 및 내분비계 교란 영향
No full text학위논문 (석사)-- 서울대학교 보건대학원 : 보건대학원 환경보건학과 환경보건 전공, 2016. 2. 최경호.2-Ethylhexyl 4-methoxycinnamate (EHMC) is one of the most frequently used UV-filters in personal care products (PCPs). Due to its widespread use, EHMC has been detected in aquatic biota as well as in surface waters. Despite its presence in aquatic environment, limited information on its toxicity in aquatic biota is available, especially in fish. In the present study, reproductive toxicity of EHMC and its underlying mechanisms were evaluated using Japanese medaka (Oryzias latipes), especially focusing on sex and thyroid hormone disruption. To fully understand the toxicity, a life-cycle test was conducted. Eggs within were exposed to 0, 0.05, 0.158, 0.5, 1.58, or 5 mg/L of EHMC. At juvenile stages of F0 generation, transcription of genes related to sex and thyroid hormone disruption was quantified. At adult stages, the fish were mated and the eggs were counted every day. At 5 months post-hatch (mph), genes related to sex and thyroid hormone disruption were analyzed in the liver and gonad of the F0 fish. Spawned eggs (F1 fish) were collected and used for early-life stage (ELS) toxicity test. In adult stages, plasma 17β-estradiol (E2) levels were not altered in both adult male and female. Decreasing patterns of transcript levels for steroidogenesis-related genes in ovary were observed. At juvenile stages of F1 generation, thyroid hormones and transcription of genes related to sex and thyroid hormone disruption was quantified. Significant decreased reproductive performances were observed observed in all treatment groups at as low as 0.05 mg/L, i.e., environmentally relevant concentration. Significant down-regulations of dio2 transcripts were observed at juvenile stages (31 day post-hatch (dph)) in both F0 and F1 generations. Whole-body triiodothyronine (T3) concentrations were significantly decreased and decreasing patterns of whole-body thyroxine (T4) concentrations, not between-group differences, were observed at juvenile stages of F1 generation. Our observations indicate that long-term exposure to EHMC can disrupt overall reproductive health outcomes and thyroid homeostasis during juvenile stages.1. Introduction 1
2. Materials and Methods 4
2.1 Chemicals 4
2.2 Maintenance of fish and experimental design 4
2.3 RNA isolation and quantitative real-time polymerase chains reaction (qRT-PCR) 7
2.4 Hormone measurement 8
2.5 Statistical analysis 9
3. Results 10
3.1 Growth effects 10
3.2 Thyroid hormones and transcriptional alterations in genes related to thyroid hormone 14
3.3 Sex hormone and transcriptional alterations in genes related to sex hormone and steroidogenesis 18
3.4 Effects of EHMC on fecundity 23
4. Discussion 25
References 30
국문초록 36Maste
Photocathode Integrated with NiO Hole‐Selective Layer for Improved Photoelectrochemical Water Splitting
No full textCuBi2O4(CBO) has received considerable attention owing to its ideal optical bandgap and positive photocurrent onset potential. However, CBO photocathodes exhibit poor charge carrier separation and transfer across the conducting substrate interface. Herein, a systematic incorporation of Ag+-cations into nanosized porous CBO network (ACBO) using a simple pulsed-electrodeposition method. In ACBO photocathode, the Ag+-ions replace the Bi3+-ions, thereby building an increased hole concentration, which further signifies the photogenerated electron-hole separation. Additionally, introducing a low-cost NiO hole-selective layer between ACBO and the conducting substrate enables a back-interface-aided hole-extraction and electron blocking, resulting in an improved charge transfer across the back interface. Compared with an unmodified CBO, the NiO/ACBO photocathode exhibits a three-fold enhanced photocurrent performance. This enhances the photocurrent originating from the incorporation of a substantial amount of Ag+-ions into the CBO structure, leading to an increased acceptor density as well as the formation of an appropriate hole-selective layer across the back-contact. The absorption percentage, time-resolved photoluminescence, and photoelectrochemical impedance spectroscopy measurements unveil the potential light harvesting, charge separation, and transfer characteristics of the NACBO photoelectrode, respectively. Through this systematic study, an efficient and simple strategy is determined for developing ternary metal oxide-based photocathode/photoanode systems for sustainable energy applications.
Rapid and Sensitive Detection of Foodborne Pathogens Using Bio-Nanocomposites Functionalized Electrochemical Immunosensor with Dielectrophoretic Attraction
No full textThe rapid detection and identification of potentially harmful microorganisms in food are essential to prevent foodborne outbreaks and ensure our safety. The faster response time and relatively high sensitivity and selectivity of biosensor-based detection methods, as compared to conventional methods, have increased the attention towards alternative approaches for the early inspection of foodborne pathogens in a variety of food products. The recent advances in micro- and nanotechnologies are attributed to the improvement of the sensor’s performance. The methods of using single-walled carbon nanotubes (SWCNTs) to enhance the sensing signal response, as well as dielectrophoresis (DEP) and fluidics techniques to improve bioaffinity reactions, create unique bio-nano sensing devices for fast and reliable microbial analysis. The goal of this study was to develop SWCNT functionalized electrochemical immunosensors for the rapid and sensitive detection of foodborne pathogens assisted with dielectrophoretic and fluidic technologies. The biosensor design, which involved electrode configuration, electrode surface modification, and detection mode, were gradually transformed to achieve the sensitive, selective, specific, or simultaneous detection of bacteria and viruses. The functionalized microwire-based electrochemical immunosensor (MEI sensor) was designed and fabricated for the selective detection of target bacteria from non-target bacteria. The Escherichia coli specific MEI sensor was prepared to test the functionalization process and the Staphylococcus aureus specific MEI sensor was used to validate the proposed sensor concept for other bacteria. The combination of double-layered SWCNTs and 5% bovine serum albumin coating contributed to signal enhancement and cell binding specificity. The selective capture of E. coli or S. aureus cells was achieved when the electric field was generated at a frequency of 3 MHz and 20Vpp. A linear trend in the change of electron transfer resistance (ΔRet) was observed as E. coli concentrations increased from 5.32 × 102 to 1.30 × 108 CFU/mL (R2 = 0.976) and S. aureus concentrations from 8.90 × 102 to 3.45 × 107 CFU/mL (R2 = 0.983). Both MEI sensors could detect target bacteria cells without interfering with the other bacteria in the mixed suspensions. The detection time was 10 min including cell concentration and signal measurement. The developed MEI sensor was evaluated for its detection of target bacteria from non-target materials in food. The E. coli specific sensor and the Salmonella specific sensor were fabricated to individually detect E. coli K12 and S. Typhimurium contaminated baby spinach. The estimated concentrations of E. coli in the spinach extracts corresponded well with the concentrations determined by the plate counting method, with an R2 value of 0.972 and a detection range from 8.33 × 102 to 7.97 × 105 CFU/g for the surface contamination method. A linear relationship was observed between ΔRet and S. Typhimurium concentrations from 1.43 × 103 to 1.67 × 107 CFU/g with an R2 value of 0.942. Both E. coli and Salmonella MEI sensors are specific towards the target bacteria in the sample despite the interference of spinach debris and non-target bacteria. The continuous flow multi-junction biosensor was fabricated and characterized for the simultaneous detection of E. coli and S. aureus. The developed continuous flow junction sensor showed an increase of sensing sensitivity by a factor of 10 in the detection of E. coli K12, as compared to the stationary sensor. A linear regression was observed for both the E. coli and S. aureus functionalized multi-junction array sensors with a detection range of 102 to 105 CFU/mL. Multiplexed detection of bacteria at sensing levels as low as 102 CFU/mL for E. coli K12 and S. aureus were accomplished within 2 min. Lastly, the flow-based dielectrophoretic biosensor was designed and tested for the detection of bacteriophage MS2 as a norovirus surrogate. The cyclic voltammogram showed that the current for the PEI-SWCNTs electrode was higher than the current of the PEI film surface, and was followed by a decrease in the current after antibody immobilization and MS2 attachment. Antibody immobilization on the detector with electric field applied to the fluidic channel at 10 Vpp and 1 MHz showed higher current changes by antibody-MS2 complexes than the assay without antibody immobilization and DEP. The changes in current signal displayed a dependence on the concentration of MS2 in the sample solution. The total assay was completed within 15 min. The developed MEI sensor with DEP-assisted cell trapping has the potential for fast, simple, and selective detection of low levels of target bacteria in the presence of mixed bacteria communities and food matrices. The CNTs functionalization and continuous flow assay could offer advances in sensitivity and detection time. The proposed sensing technology and the device can have a beneficial influence on the food industry by offering the rapid detection of multiple pathogens in food. It will also result in the development of new approaches to monitor and control biological hazards, which can be incorporated into food production and processing facilities to improve the safety of our food products.Ph.D. Thesis. University of Hawaiʻi at Mānoa 2017
Taxonomic review of the family Onychiuriae (Poduromorpha: Collembola) from the Korean Peninsula
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