347,666 research outputs found
Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis
No full textStudies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies
Lea, R S, QX16410
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/398710Surname: LEA. Given Name(s) or Initials: R S. Military Service Number or Last Known Location: QX16410. Missing, Wounded and Prisoner of War Enquiry Card Index Number: 32752.215979
Item: [2016.0049.31003] "Lea, R S, QX16410
The River Lea 1571-1767: a river navigation prior to canalisation
No full textPhDIn pre-industrial England river navigations were subject
to improvement by canalisation, the introduction of
artificial navigation cuts and pound locks. Along the Lea
this did not happen until 1767. Before that the navigation,
except for one short period, relied upon a less efficient
technology, the provision of flashes from fishing weirs,
turnpikes and mills. Yet the river was still an important
transport route, particularly for the supply of grain, meal
and malt to London. It had been this during the mediaeval
period, but not by the middle of the sixteenth century. Then
in 1571 the City of London sponsored legislation to construct
a canal from the Lea to London. Parliamentary opposition
thwarted the original ambitious scheme, so two cheaper,
shorter canals were considered, but never built. Instead
an ambitious and unique river improvement scheme was
successfully implemented. This experimental navigation
(reducing reliance on flashes to a minimum) survived 20
years, before persistent and violent opposition from land
carriers closed it. A Star Chamber case upheld the rights
of the bargemen, but the experimental navigation was not
restored. Instead the traditional flash-lock navigation
re-appeared, and was to last, with only minor improvementg
until 1767. In the intervening years the navigation
continued to expand and prosper., This despite the admitted
problems of relying on flashes and tides, and despite a
series of major disputes with the New River Companyq the
millers, fishermen and riparian land-owners. Conflict there
certainly was, but also compromise. Ultimately all parties
were prepared to accept the conflicting rights of other
users, provided they could defend their own. commissions
of Sewers provided an effective administrative forum to
effect and authorise such compromise, even after the
appointment of a body of Trustees in 1739. That the Lea
was an adequate navigation before canalisation, despite
a 'second-best' technology and an unpaid part-time administrative
structure means' that a valid comparison with the
concept of Appropiate Technology, discussed in modern-day
development theory, is possible
Utvärdering av verktyget Lokalekonomisk analys, LEA
No full textFör att utveckla landsbygden behövs fungerande verktyg att arbeta utifrån. Lokalekonomisk analys, LEA, är ett sådant verktyg som funnits i snart femton års tid. En Lokalekonomisk analys ger statistik över exempelvis in- och utflyttning, befolkning och disponibel inkomst. Verktyget beställs och köps av Statistiska centralbyrån. Som med de flesta verksamheter behöver LEA uppdateras för att kunna bemöta de behov som finns och anpassas till den teknik som är tillgänglig i dagsläget. Uppsatsen är en förklarande och till viss del värderande utvärdering. Utifrån orsak – verkan samband undersöker jag hur väl LEA fungerar som verktyg för landsbygdsutveckling.
Utvärderingen är baserad på intervjuer med personer som på olika sätt har varit i kontakt med, och arbetat med LEA. Den teoretiska utgångspunkten är utvärderingsmetodik samt en jämförelse av empirin med teorier om socialt kapital enligt Putnam och Bourdieu.
Studien visar att det finns ett visst behov av uppdatering av LEA. Den behöver moderniseras och bli mer lättillgänglig. Flertalet av de intervjuade önskar se ett nästa steg i hur statistiken sedan kan sättas i verket och bli till en konkret handlingsplan. Informanterna anser att kommunen kan bli mer involverad och eventuellt vara med och medfinansiera beställda Lokalekonomiska analyser inom kommunen.
Det sociala kapitalet har påverkan på utfallet av en lokal utvecklingsgrupps resultat. Studien stärker därmed Putnams och Bourdieus teorier kring socialt kapital.In order to work for rural development different functional tools are required. LEA, Local Economic Analysis is such a tool that has existed for nearly fifteen years. LEA provides statistics over a certain area, such as population, disposable income and of people moving in and out. The tool can be purchased from the Swedish Statistics Bureau. As with most tools, LEA needs to be updated to respond to what is requested and from the available technology. This essay is an evaluation of LEA and explains the cause – effect connection of the results. It explains to what extent the results turned out like the objectives of the LEA as a tool for rural development. The evaluation is based on empirical data from interviews with people that in different ways have been in contact with, and worked with LEA. The theoretical basis is evaluating methodology and a comparison of empirical data with theories of social capital according to Putnam and Bourdieu.
The study shows that there is need for updating LEA. There is a need to modernize and LEA could become more accessible. Most of those interviewed wanted to see a next step in how the specified statistics then can be put into use and become an action plan. The municipality´s role could according to the informants be more visible and perhaps co-finance the cost of a LEA.
Social capital has a major impact on the outcome of a development group´s results. The study therefore reinforces Putnam´s and Bourdieu´s theories on social capital
[Correspondence from N. Lea to Margaret Houston] - April 2, 1862
No full textThese materials, which were collected by the Honorable Temple Houston Morrow, grandson of Samuel and Margaret Lea Houston, reveal the life of the Sam Houston Family through letters, which were composed principally by Mrs. Houston; and through assorted documents and receipts, which recorded some of the business transactions of the family during that era. Sam Houston's correspondence is from the period when he served Texas as a United States Senator and as Governor of the state. It also includes some letters written by Sam Houston, Jr., when he served with a confederate artillery battery in Louisiana
Microrhopaea flavipennis Lea N. Territory 1920
No full textMicrorhopaea flavipennis Lea, 1920 Microrhopaea flavipennis Lea, 1920: 737. Lectotype (here designated) male (Figs. 51–53): mounted on card | flavipennis (handwritten) Lea, TYPE (typeset) Darwin [12.46°S, 130.84°E] (handwritten) | 19663 Microrhopaea flavipennis Lea N. Territory (handwritten) TYPE (handwritten in red, at right angle) | SAMA Database No. 25-035326 | my lectotype label; in SAM. Paralectotypes: 5 males mounted on two cards on the same pin | 7/10 | Port Darwin N. Territory typeset) (two identical labels) | Microrhopaea flavipennis Lea E.B. Britton det 1955 (handwritten and author name typeset) | my paralectotype label; in SAM. Lea (1920) did not define the type series, seeing an unknown number of males (vide Articles 73.1.2, 72.1.1) and did not designate a register number for the type or indicate where the specimen(s) were housed. Britton (1978) referred to a “ holotype ” male and a “ paratype ” male from Darwin in SAM, and Houston & Weir (1992) considered this incorrect reference to a holotype as being a lectotype designation. As Britton (1978) and Houston & Weir (1992) provided no discussion and did not label any specimen in SAM, neither mention of a holotype constitutes a valid lectotype designation (vide Article 74.5). To stabilise nomenclature, I designate the specimen in SAM with the ‘TYPE’ label the lectotype. The other five specimens in SAM are paralectotypes.Published as part of Allsopp, Peter G., 2020, Clarification of the status of the types of Australian Melolonthini (Coleoptera Scarabaeidae: Melolonthinae) described before 1950, pp. 451-486 in Zootaxa 4885 (4) on page 473, DOI: 10.11646/zootaxa.4885.4.1, http://zenodo.org/record/429684
Cloning and characterisation of LEA1-EM genes in the resurrection plant, Xerophyta humilis
No full textIncludes abstract.Includes bibliographical references (leaves 75-86).The presence and expressIon patterns of orthologues of LEA group 1 genes has been characterised in the resurrection plant, Xerophyta humilis. The group I LEAs (Em I and Em6) were first identified as proteins that were abundantly and specifically expressed during the desiccation and germination phase of angiosperm seed development. The group I LEA genes are characterised by the presence of one or more tandemly repeated 20-amino acid motifs that are particularly rich in Gly residues
Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis)
No full textLate embryogenesis abundant (LEA) proteins have been identified in a wide range of organisms and are believed to play a role in the adaptation of plants to stress conditions. In this study, we performed genome-wide identification of LEA proteins and their coding genes in Moso bamboo (Phyllostachys edulis) of Poaceae. A total of 23 genes encoding LEA proteins (PeLEAs) were found in P. edulis that could be classified to six groups based on Pfam protein family and homologous analysis. Further in silico analyses of the structures, gene amount, and biochemical characteristics were conducted and compared with those of O. sativa (OsLEAs), B. distachyon (BdLEAs), Z. mays (ZmLEAs), S. bicolor (SbLEAs), Arabidopsis, and Populus trichocarpa. The less number of PeLEAs was found. Evolutionary analysis revealed orthologous relationship and colinearity between P. edulis, O. sativa, B. distachyon, Z. mays, and S. bicolor. Analyses of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that the duplication of PeLEAs may have occurred around 18.8 million years ago (MYA), and divergence time of LEA family among the P. edulis-O. sativa and P. edulis-B. distachyon, P. edulis-S. bicolor, and P. edulis-Z. mays was approximately 30 MYA, 36 MYA, 48 MYA, and 53 MYA, respectively. Almost all PeLEAs contain ABA-and (or) stress-responsive regulatory elements. Further RNA-seq analysis revealed approximately 78% of PeLEAs could be up-regulated by dehydration and cold stresses. The present study makes insights into the LEA family in P. edulis and provides inventory of stress-responsive genes for further functional validation and transgenic research aiming to plant genetic improvement of abiotic stress tolerance
ER5, a tomato cDNA encoding an ethylene-responsive LEA-like protein: characterization and expression in response to drought, ABA and wounding
No full textWe report the isolation by differential display of a novel tomato ethylene-responsive cDNA, designated ER5. RT-PCR analysis of ER5 expression revealed an early (15 min) and transient induction by ethylene in tomato fruit, leaves and roots. ER5 mRNA accumulated during 2 h of ethylene treatment and thereafter underwent a dramatic decline leading to undetectable expression after 5 h of treatment. The full-length cDNA clone of 748 bp was obtained and DNA sequence analysis showed strong homologies to members of the atypical hydrophobic group of the LEA protein family. The predicted amino acid sequence shows 67%, 64%, 64%, and 61%sequence identity with the tomato Lemmi9, soybean D95-4, cotton Lea14-A, and resurrection plant pcC27-45 gene products, respectively. As with the other members of this group, ER5 encodes a predominantly hydrophobic protein. Prolonged drought stress stimulates ER5 expression in leaves and roots, while ABA induction of this ethylene-responsive clone is confined to the leaves. The use of 1-MCP, an inhibitor of ethylene action, indicates that the drought induction of ER5 is ethylene-mediated in tomato roots. Finally, wounding stimulates ER5 mRNA accumulation in leaves and roots. Among the Lea gene family this novel clone is the first to display an ethylene-regulated expression
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