995 research outputs found
Overexpression of the protein kinase CK2 increases the survival and resistance to chemotherapy of acute myeloid leukemia cells
No full textBackground: The critical role of protein kinase CK2 in the regulation of cellular apoptosis suggests its may be involvement in tumor cell resistance to both conventional and unconventional therapies.
Aim of the study: To study the role of CK2 in acute myeloid leukemia (AML) cell survival and response to chemotherapeutic agents.
Methods: Apoptotic pathways were evaluated by AnnexinV/Propidium Iodide staining, Western Blot analysis of Caspase3 and Parp cleavage and Real Time PCR of antiapoptotic gene expression.
Results: CK2 α catalytic subunit expression level and activity were increased in AML cells as compared to normal CD34+ hematopoietic cells. CK2 inactivation with the synthetic chemical inhibitors K27, CX-4945 or RNA interference induced AML cell apoptosis of p53 wild-type but not of p53-null cells, suggesting that the apoptosis triggered by CK2 inhibition needs the presence of functional p53. Inhibition of CK2 activity with K27 or CX-4945 was associated to an increased sensitivity of the cytotoxic effects of doxorubicin and daunorubicin. Cells were also nucleofected with siRNA oligos directed against CK2 α catalytic, β regulatory or both subunits. Interestingly RNA interference of CK2 β reduced cell viability and enhanced apoptosis induced by daunorubicin, indicating a prominent role of this subunit in the resistance to chemotherapy.
Based on our in-vitro results we have then examined the expression level of CK2 α and β in 32 newly diagnosed AML patients (excluding acute promyelocitic leukemia) from 2008 to 2011. Preliminary data shows a higher expression of the CK2 α subunit in the two AML subgroups with the highest risk based on cytogenetic characteristics. These laters nowadays represent the strongest prognostic factor predicting chemoresistance. Interestingly we did not find a correlation with expression of the regulatory subunit CK2 β.
Conclusions: These data highlight the relevance of CK2 in AML cell survival for the influence of this kinase on the activation of anti-apoptotic pathways implicated in AML cell resistance to chemotherapy.
A study to establish wether the CK2 expression at diagnosis represents an independent prognostic factor in the progression free survival and overall survival for these patients is needed
«Qualche cosa che non si stanca mai di lasciarsi definire». Il paesaggio tra arte e natura in Andrea Zanzotto
No full textCalidad de carne desde la mirada de los consumidores
No full textLas preferencias y las preocupaciones de los consumidores, asociadas a la calidad de la carne, su impacto en el medioambiente, su salud y las características de ética de la producción, definen el consumo de carne bovina. Conocer las expectativas de los consumidores nos permite establecer estrategias para su promoción.Instituto de Tecnología de AlimentosFil: Grigioni, Gabriela María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.Fil: Grigioni, Gabriela María. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.Fil: Testa, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Testa, María Laura. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Soteras, Trinidad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.Fil: Soteras, Trinidad. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.Fil: Pavan, Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Pavan, Enrique. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Pavan, Enrique. Massey University Campus. Te Ohu Rangahau Kai. AgResearch. Limited; Nueva Zeland
Chi si fida del vaccino anti-Covid? Infodemia, percezione di «information overload» sui social media e polarizzazione ideologica
No full textThis paper provides empirical evidence about varying levels of trust in anti-Covid vaccines amongst Italian citizens. We problematize the issue of trust at the crossroads between three strands of literature. First, extant reflections on «infodemics» – i.e., the massive circulation of often dubious or fake information about health matters. Second, literature on information overload on social media, and users’ willingness to process all the information they are exposed to on these platforms. Third, studies on ideological polarization and its implications for the political management of the pandemic but also for citizens’ behaviors and attitudes towards it. More specifically, we hypothesize a positive association between higher levels of trust in Covid-19 vaccine and knowledge about the pandemic based on the information shared by institutional sources. We also hypothesize a negative association between trust in the vaccine and a perception of over-exposition to information on social media. Finally, we hypothesize the existence of an ideological trust gap for right-wing citizens and, to a lesser degree, for those who refuse to locate themselves on the ideological axis, who all express lower trust levels than left-wingers. Our analysis leans on unique data from a sample of adult internet users surveyed in December 2020. Results confirm that lower knowledge levels and the perception of being over-exposed to information on social media reduced trust in vaccines. Moreover, they show the existence of a polarized scenario in which left-wing citizens were more trustful in vaccines than other
Assessment of chromosomal rearrangements helps to differentiate multiple lung primary cancers from metastases
No full textAnimal performance and meat quality characteristics from feedlot-finished steers fed increasing levels of wet distillers grain
No full textOne hundred forty-four steers were group-housed in 24 pens that were randomly assigned to one of four dietary treatments defined by the proportion of wet distiller grain plus solubles (WDGS; 0, 15, 30, or 45%) and fed for 84 d pre-slaughter. Animal performance was evaluated using the pen as the experimental unit. Whereas for carcass and meat quality characteristics, meat oxidative stability, and the consumer sensory quality of longissimus thoracis muscle one animal from each pen was randomly selected and used as the experimental unit. No differences (P > 0.05) were observed for subcutaneous fat thickness, rib eye area, marbling score or pH, color parameters, proximate composition, sarcomere length, Warner Bratzler shear force, and cooking loss. Feeding WDGS linearly increased total PUFA (P = 0.05), C18:2 n-6 (P = 0.004) proportions, and n-6/n-3 ratio (P < 0.01) but reduced C16:1 to C18:0 ratio (P < 0.01). Lipid oxidation was greater in beef from steers fed 30% and 45% WDGS (P = 0.05). Dietary WDGS linearly improved (P < 0.05) flavor and overall linking score in the consumer sensory panel.EEA BalcarceFiL: Pouzo Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.FiL: Pouzo Laura Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Ceconi, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria General Villegas; Argentina.Fil: Davies, Patricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria General Villegas; Argentina.Fil: Méndez, Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria General Villegas; Argentina.Fil: Ortiz Miranda, S.G. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Testa, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Pavan, Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Pavan, Enrique. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Pavan, Enrique. Clemson University. Department of Animal and Veterinary Science; Estados Unidos
Living in an extremely polluted environment: Clues from the genome of melanin-producing Aeromonas salmonicida subsp. pectinolytica 34melT
No full textAeromonas salmonicida subsp. pectinolytica 34mel(T) can be considered an extremophile due to the characteristics of the heavily polluted river from which it was isolated. While four subspecies of A. salmonicida are known fish pathogens, 34mel(T) belongs to the only subspecies isolated solely from the environment. Genome analysis revealed a high metabolic versatility, the capability to cope with diverse stress agents, and the lack of several virulence factors found in pathogenic Aeromonas. The most relevant phenotypic characteristics of 34mel(T) are pectin degradation, a distinctive trait of A. salmonicida subsp. pectinolytica, and melanin production. Genes coding for three pectate lyases were detected in a cluster, unique to this microorganism, that contains all genes needed for pectin degradation. Melanin synthesis in 34mel(T) is hypothesized to occur through the homogentisate pathway, as no tyrosinases or laccases were detected and the homogentisate 1,2-dioxygenase gene is inactivated by a transposon insertion, leading to the accumulation of the melanin precursor homogentisate. Comparative genome analysis of other melanogenic Aeromonas strains revealed that this gene was inactivated by transposon insertions or point mutations, indicating that melanin biosynthesis in Aeromonas occurs through the homogentisate pathway. Horizontal gene transfer could have contributed to the adaptation of 34mel(T) to a highly polluted environment, as 13 genomic islands were identified in its genome, some of them containing genes coding for fitness-related traits. Heavy metal resistance genes were also found, along with others associated with oxidative and nitrosative stresses. These characteristics, together with melanin production and the ability to use different substrates, may explain the ability of this microorganism to live in an extremely polluted environment
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