1,721,003 research outputs found
An induced pluripotent stem cell (iPS)-based approach for the repopulation and phenotypic correction of cystic fibrosis airway epithelium
No full textBackground. In cystic fibrosis, the repopulation of diseased airway epithelium with cells expressing functional CFTR represents an option that may be available in the future to reduce morbidity and mortality of this disease.
The recently developed induced pluripotent stem cells (iPS) allowed the generation of patient-specific stem cells that may be useful for cell therapy approaches.
For instance, in cystic fibrosis, diseased lung epithelium could potentially be replaced by functional epithelial cells derived from iPS. iPS could be generated from the patient’s somatic cells, and the gene defect could be corrected in vitro. Then, iPS could be induced to differentiate in vitro into airway epithelial cells and these cells could be infused to the same patient, thus avoiding immune rejection, to repopulate the diseased airway epithelium with normal cells.
This is a very ambitious goal, and many issues need to be solved before this approach becomes applicable to human treatment in the future.
The induction of differentiation of iPS into bronchial epithelial cells still represents a difficult task, since our knowledge of the mechanisms that guide this differentiation process are still limited.
Recent studies suggest that iPS cells derived from airway epithelial cells may possess a stronger differentiation potential towards airway epithelial lineages than iPS cells derived from other cell types.
Objectives. Therefore we proposed to derive iPS cells from airway epithelial cells, either normal or from patients affected by cystic fibrosis
Project status. Within the first year of the project we induced the reprogramming of human airway epithelial cells, normal and CF, that should determine the development of iPS cells.
We are now at the end of the first year of the project and we are involved in the analysis of the cell cultures for the presence of iPS colonies, that will be isolated and characterized.
During the second year we will induce the differentiation of iPS cells into airway epithelial cells.
Spin-off for research & clinical purposes. This study should clarify whether iPS cells derived form airway epithelial cells have a higher differentiation potential towards lung epithelium.
This knowledge is relevant for a potential future application of iPS cells in the repopulation of CF lung epithelium with functional cells
Per un'edizione dell'epistolario di Luigi Pirandello
No full textThe epistolary corpus of Luigi Pirandello, initially thought to be slim, following the research, studies and discoveries of the last thirty years has instead turned out to be very considerable.This research work consists in the collection, census, reorganisation and systemisation of all the letters published by Pirandello over fifty years (1886-1836) through the comparison of the various printed editions. A further aim is to contribute to the recomposition of Pirandello's epistolary corpus by integrating all the extant material available to date. This material has numerous diverse sources, from monographs to articles in specialist magazines and journals and so has been organized according to thematic criteria or relative to limited periods of time.The result is a voluminous preparatory work for the realization of a timely updated philological edition of the epistolary production
Derivation of normal and cystic fibrosis human induced pluripotent stem cells (iPSCs) from airway epithelium.
No full textChronic treatment with SCH 23390 increases the production rate of dopamine D1 receptors in the nigro-striatal system of the rat
No full textLung injury by silica inhalation does not enhance recruitment of bone marrow-derived cells to lung epithelium.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
CFTR mRNA is expressed in lungs of CFTR-KO mice following administration of adult marrow cells from wild-type donor mice.
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