14 research outputs found
The effect of Tokopedia marketplace promotion on consumer shopping behavior in the COVID-19 pandemic: Case study on Tokopedia marketplace users at Pesona Depok Estate Housing RT 002 RW 022
The government's COVID-19 policy limits the actions of all Indonesians. People's buying habits have shifted to internet shopping via increasing marketplaces. This is why product-producing enterprises and MSMEs use the Marketplace. Markets use promotional methods to attract internet customers. The author wants to know how Tokopedia's marketplace promotion affected consumer shopping behavior during the COVID-19 epidemic. This research employs the Consumer Behavior Theory to investigate a consumer's decision-making process. This quantitative research employs linear regression analysis. Pesona Depok Estate dwelling RT 002 RW 022, Depok, was the study site and sample. Purposeful sampling. A predefined number of samples received a Google Form questionnaire. The coefficient of determination (R2) is 0.610, which suggests Tokopedia's marketing affected 61% of customer purchase behavior during the COVID-19 epidemic. Shopee's advertising affected customer purchase behavior during the COVID-19 epidemic
Pengaruh Terpaan Tayangan Religi di Televisi Terhadap Sikap dan Perilaku Taqwa Pemirsa
This research entitled Influence of Impression on Religious Display on Television (Trans TV) on attitude and behavior of Taqwa Pemirsa (Student of Attahiriah Islamic University), which aims to 1) to know about exposure of religious event in Trans TV to viewers, ie Attahiriah University students, 2) Attitudes and behaviors of the audience, and 3) knowing whether the religious event's exposure to the audiences affected their attitude and piety behaviors. Research using correlation method using sampling technique, purposive sampling. Which with the formula Yamane obtained 98 respondents from the University Attahiriyah. Data collection tools used are questionnaires, observation and literature study. This research uses S-O-R model (stimulus-organism-response) and AIDDA (Attention, Interest, Desire, Decision, Action). Model S-O-R to see the effect of stimuli in the form of religious impressions to the audience (organisms) in which the response is the attitude and behavior of taqwa. While the AIDDA Model to see whether the attention of the attention will generate interest, then desire, then influence the decision and action as conveyed in the message in the show, such as attitude and Taqwa behavior. Research shows the relationship between variables X and variable Y is a high correlation between religious impressions Impression on attitudes and behavior of university students attitudes taqwa. After calculation using correlation Pearson's correlation formula (product moment) obtained correlation coefficient value of 0.79. So that the influence of exposure to religious impressions is important to create or foster attitudes and behavior of the audience, especially students attahiriyah
Pengaruh Terpaan Tayangan Religi di Televisi Terhadap Sikap dan Perilaku Taqwa Pemirsa
This research entitled Influence of Impression on Religious Display on Television (Trans TV) on attitude and behavior of Taqwa Pemirsa (Student of Attahiriah Islamic University), which aims to 1) to know about exposure of religious event in Trans TV to viewers, ie Attahiriah University students, 2) Attitudes and behaviors of the audience, and 3) knowing whether the religious event's exposure to the audiences affected their attitude and piety behaviors. Research using correlation method using sampling technique, purposive sampling. Which with the formula Yamane obtained 98 respondents from the University Attahiriyah. Data collection tools used are questionnaires, observation and literature study. This research uses S-O-R model (stimulus-organism-response) and AIDDA (Attention, Interest, Desire, Decision, Action). Model S-O-R to see the effect of stimuli in the form of religious impressions to the audience (organisms) in which the response is the attitude and behavior of taqwa. While the AIDDA Model to see whether the attention of the attention will generate interest, then desire, then influence the decision and action as conveyed in the message in the show, such as attitude and Taqwa behavior. Research shows the relationship between variables X and variable Y is a high correlation between religious impressions Impression on attitudes and behavior of university students attitudes taqwa. After calculation using correlation Pearson's correlation formula (product moment) obtained correlation coefficient value of 0.79. So that the influence of exposure to religious impressions is important to create or foster attitudes and behavior of the audience, especially students attahiriyah
Pengaruh Terpaan Tayangan Religi di Televisi Terhadap Sikap dan Perilaku Taqwa Pemirsa
This research entitled Influence of Impression on Religious Display on Television (Trans TV) on attitude and behavior of Taqwa Pemirsa (Student of Attahiriah Islamic University), which aims to 1) to know about exposure of religious event in Trans TV to viewers, ie Attahiriah University students, 2) Attitudes and behaviors of the audience, and 3) knowing whether the religious event's exposure to the audiences affected their attitude and piety behaviors. Research using correlation method using sampling technique, purposive sampling. Which with the formula Yamane obtained 98 respondents from the University Attahiriyah. Data collection tools used are questionnaires, observation and literature study. This research uses S-O-R model (stimulus-organism-response) and AIDDA (Attention, Interest, Desire, Decision, Action). Model S-O-R to see the effect of stimuli in the form of religious impressions to the audience (organisms) in which the response is the attitude and behavior of taqwa. While the AIDDA Model to see whether the attention of the attention will generate interest, then desire, then influence the decision and action as conveyed in the message in the show, such as attitude and Taqwa behavior. Research shows the relationship between variables X and variable Y is a high correlation between religious impressions Impression on attitudes and behavior of university students attitudes taqwa. After calculation using correlation Pearson's correlation formula (product moment) obtained correlation coefficient value of 0.79. So that the influence of exposure to religious impressions is important to create or foster attitudes and behavior of the audience, especially students attahiriyah.</jats:p
TRAINING ON THE USE OF INSTAGRAM AS A MARKETING COMMUNICATION TOOL FOR MSMES IN TEBET DISTRICT
Micro, Small, and Medium Enterprises (MSMEs) play a vital role in supporting local economies in Indonesia. However, many MSMEs still face significant challenges in utilizing digital platforms effectively, particularly Instagram, as a strategic marketing communication tool. This community service program was designed to enhance the digital marketing capacity of MSMEs in Tebet District through four structured stages: socialization, training, mentoring, and evaluation.The program began with socialization to identify participants’ needs, analyze their current practices, and establish a baseline of digital literacy. The training stage provided knowledge of digital marketing communication strategies, content planning, and the use of Instagram features such as Stories, Reels, and Insights. Mentoring and assistance offered continuous guidance and personalized feedback, enabling participants to apply new knowledge in practice. Finally, evaluation measured improvements in skills, confidence, and content engagement while gathering feedback for future development.Results showed that MSMEs demonstrated significant progress in creating structured content schedules, designing consumer-oriented messages, and managing two-way communication with their customers. Businesses also reported increased engagement, stronger customer interactions, and more effective use of limited resources. These outcomes highlight that Instagram, when strategically managed, can serve as an affordable yet powerful tool for enhancing brand visibility and customer loyalty.In conclusion, the program successfully achieved its objectives by equipping MSMEs with practical digital marketing competencies. The initiative emphasizes the importance of community-based interventions in fostering digital literacy, entrepreneuria
Genomic Epidemiology of Multidrug-resistant Organisms from Humans and Food Animals
The emergence of carbapenemase-producing Enterobacteriaceae (CPE) has thwarted clinical treatment of infections caused by multidrug-resistant Gram-negative bacteria. The spread of CPE in hospitals, communities and food animals poses great challenges to public health. Comprehensive analysis with molecular typing is vital to understand the epidemiological trends and transmission patterns of antimicrobial resistance to guide infection control and prevention policies. The application of whole genome sequencing (WGS) has vastly improved the ability to elucidate the antimicrobial resistance genes involved, their transmission patterns and phylogenetic relationship among CPE isolated in various populations.The objectives of this study were (1) to describe the changing pattern and characterize CPE isolates in Hong Kong hospitals using WGS; (2) to characterize acquired virulence factors and antimicrobial resistance genes in carbapenemase-producing Klebsiella pneumoniae in a Sri Lankan hospital; (3) to screen and characterize multidrug-resistant organisms (MDROs) colonized in healthy subjects in Hong Kong; and (4) comparative genomic analysis of NDM-producing E. coli (NDM-E) from different sources (hospitalized patients, healthy subjects in community and food animals).The molecular epidemiology of 567 CPE isolates from patients of three of the seven public hospital clusters in Hong Kong in a seven-year period (2011-2017) was elucidated. The incidence of CPE isolation increased from 0.05 to 9.6 /100,000 inpatient-days. The most prevalent carbapenemase genes were blaNDM (55.1%), blaIMP (30.0%), blaKPC (8.7%), blaOXA-48-like (6.9%), and blaIMI (1.9%). E. coli (46.4%) and Klebsiella spp. (38.3%) were the dominant species. WGS was performed on 169 representative isolates. Two distinct lineages of blaKPC-2-positive Klebsiella pneumoniae (ST11 & ST258) were identified with ST11 carrying yersiniabactin gene ybt-9 on ICEKp3. ST131 E.coli producing IMP-4 was a locally transmitted clone throughout the study period. The blaNDM and blaIMP genes were mainly carried in IncX3 and IncN-ST7 plasmids respectively. BlaOXA-48-like gene was carried in IncX3 plasmid in E. coli but ColKP3 plasmid in K. pneumoniae. A lineage of K. pneumoniae with blaNDM-1 plus blaOXA-232/OXA-181 in a distinct blaNDM-embedding plasmid (IncF1B/IncHI1B) was identified and associated with patients who had prior overseas hospitalization.The prevalence of CPE in the intensive care units of a teaching hospital in Sri Lanka was studied. In a six-month period of 2015, CPE was identified in 2.6% of 379 respiratory specimens. All ten strains were Klebsiella pneumoniae-producing blaOXA-181, in which two strains of ST437 and eight isolates were ST147. Carbapenemase genes were located on three variants of ColE-type plasmids, with the longest being 7,606 bp. Genes encoding for extended spectrum β-lactamases (ESBL) (blaCTX-M-15, blaSHV-11), plasmid-mediated quinolone resistance (PMQR) determinants (qnr, aac(6')-Ib-cr and oqxAB) were present in all strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICEkp.A prevalence study on the carriage of MDROs of healthy subjects in Hong Kong was performed. The study involved longitudinal screening on the colonization of methicillin-resistant Staphylococcus aureus (MRSA), ESBL producing Enterobacteriaceae (ESBL-E) and CPE from 1076 healthy subjects over a 3-year period (2017-2019). The prevalence of MRSA was 1.2%-2.5% among the 3 years with six carriers persistently positive after one year of screening. Among 31 MRSA isolates, ST22/SCCmecIV(2B), also known as EMRSA-15 was the major lineage (29%). MRSA from the same prolonged carriers were closely related. Only one MRSA strain carried Panton-Valentine Leucocidin (PVL) genes (lukF & lukS) while another leukocidin gene, lukED, was found in 41.9% strains. ESBL-E was detected in 43.4%-54.8% subjects during the 3-year period. PCR of blaCTX-M genes revealed blaCTX-M-G9 was the most prevalent type in the 1st year and 2nd year studies while blaCTX-M-G1 dominated in the 3rd year study. CPE carriage rate ranged from 0.5% to 2% and was predominated by blaNDM-positive E. coli with the gene located in IncX3 plasmid. CPE with other carbapenemase types (OXA-181, IMI-6) were also found in healthy people.A comparative genomic analysis of 83 strains of NDM-producing E. coli (NDM-E) isolated from community dwellers, clinical patients and food animal products (pig organs from wet markets) in Hong Kong were analyzed. In addition, whole genomes of 961 NDM-E from across the globe were included in the analysis to compare the phylogeny and molecular characteristics of Hong Kong strains against the global strains of different sources and regions. IncX3 plasmid carrying blaNDM-5 prevailed in NDM-E from diverse sources in Hong Kong, but distinct clones and AMR genes were observed in NDM-E from human and food animals. The presence of phenicol-resistant genes (floR, cmlA) was significantly higher in swine-source isolates while fewer ESBL genes were observed. Mobile genetic elements (MGEs) carrying blaNDM varied across regions while blaNDM-positive IncX3 plasmid is a global menace. IncB/O/K/Z plasmids were exclusively found in Chinese farms and clinical patients. IncA/C2 plasmids were frequently carried by European strains but were not found in China. We also found that ST48 lineage was restricted to China and comprised of NDM-E from different sources in Hong Kong as well as poultry, feed and sewage in Chinese farms.In conclusion, comprehensive molecular epidemiology of CPE was described in Hong Kong. WGS-based in-depth analysis was conducted to reveal the phylogenetic relationship, MGEs, and accessory gene profiles. We established the emergence of EMRSA-15 and a high percentage of ESBL-E carriage within the healthy population of Hong Kong, while CPE was also detectable. NDM-producing Enterobacteriaceae was the most prevalent CPE from different sources. IncX3 plasmid-mediated transmission of NDM-producing Enterobacteriaceae was the major source of CPE in Hong Kong and worldwide.產碳青霉烯酶腸道桿菌(CPE)的出現阻礙了多重耐藥細菌感染的治療。CPE在醫院,社區以及動物中的傳播對於公共衛生而言是嚴重的威脅。全面的分子生物學分型對理解流行趨勢和傳播方式以及為感染防控政策的制定起著至關重要的作用。運用全基因組測序(WGS)能極大的提高對耐藥基因檢測,傳播途徑分析,以及不同集群的種系發育研究的能力。此次研究的目的如下:(1)利用WGS分析描述香港不同醫院中CPE的變化模式以及特徵;(2)分析斯里蘭卡醫院中產碳青霉烯酶肺炎克雷伯菌獲得性毒力因子和耐藥基因;(3)篩查並分析香港健康人群中定植的多重耐藥菌;(4)對不同來源的產NDM酶的大腸桿菌進行比較基因組學研究。我們對七年間來自香港三個公立醫院群(總計七個)的病人樣本中分離到的567株CPE進行分子流行病學分析。CPE的分離率從0.05增長到9.6每10000住院病人日次。最常見的碳青霉烯酶基因包括blaNDM (55.1%),blaIMP (30.0%),blaKPC (8.7%),blaOXA-48-like (6.9%) 和 blaIMI (1.9%)。大腸桿菌(46.4%) 和克雷伯氏菌 (38.3%) 是最常見的種屬。我們對169株代表性的菌株進行了全基因組測序。我們發現攜帶blaKPC-2的肺炎克雷伯氏菌分布於兩個不同世系(ST11和ST258),其中ST11的菌株攜帶位於ICEKp3耶尔西菌素基因ybt-9。我們發現屬於ST131 的攜帶IMP-4的大腸桿菌為本地流行克隆型。blaNDM 和 blaIMP 基因主要分別由IncX3和IncN-ST7型質粒攜帶,分布於不同分子分型的大腸桿菌,肺炎克雷伯氏菌和不同種屬間。blaOXA-48-like基因在大腸桿菌中位於IncX3型質粒上,而在肺炎克雷伯氏菌中位於ColKP3型質粒上。同時產blaNDM-1和blaOXA-48-like 基因的肺炎克雷伯氏菌中,blaNDM-1的質粒屬於另一類型質粒(IncF1B/IncHI1B),並且該世系與病人過往海外入院歷史有關。我們分析了斯里蘭卡一間醫院的重症監護室中CPE的流行情況。在2015年的六個月中,從379份呼吸道樣本中篩查出2.6%的CPE。所有的十株肺炎克雷伯氏菌(兩個屬於ST437,八個屬於ST147)均產blaOXA-181基因。碳青霉烯酶基因分佈於三種ColE型變種質粒上,最長的是7606bp。廣效性乙內醯胺酶(ESBL)基因(包括blaCTX-M-15, blaSHV-11)以及質粒介導的奎諾酮類耐藥基因(qnr, aac(6')-Ib-cr和 oqxAB)存在於所有的菌株中。我們觀察到在染色體的奎諾酮類耐藥決定區域存在gyrA (Ser83Ile) 和 parC (Ser80Ile) 兩個基因的點突變。所有的菌株都攜帶位於ICEKp上的耶尔西菌素基因ybt。在香港健康人群中,我們研究了MDRO的流行情況以及分析了分離到的菌株。該長期研究對三年間總計1076位健康人群所攜帶的抗藥性金黃葡萄球菌(MRSA),產ESBL的腸桿菌(ESBL-E)和CPE的情況進行了調查。MRSA攜帶率介於1.2%-2.5%之間並且有六位長期攜帶者,一年後的篩查結果仍為陽性。在分離到的31株MRSA中, ST22/SCCmecIV(2B),也被稱為EMRSA-15是最主要的世系(29%)。從同一個長期攜帶著分離到的MRSA表現出高度相關。只有一株MRSA攜帶Panton-Valentine Leucocidin (PVL) 基因(lukF&lukS),但是有41.9%的菌株攜帶另一種殺白血球素 lukED。ESBL-E在43.4%-54.8%調查對象中被發現。對於blaCTX-M基因,分析顯示在預試驗以及第一輪篩查中,blaCTX-M-G9是最常見的分型,但是在第二輪篩查中blaCTX-M-G1佔主要分佈。在所有的172位長期ESBL攜帶者中,僅19.1%的人持續攜帶同一類型的blaCTX-M。CPE的攜帶率介於0.5%與2%之間且主要為攜帶IncX3型blaNDM陽性質粒的大腸桿菌。產其他的碳青霉烯酶的CPE(OXA-181, IMI-6)也在健康人群中有發現。利用比較基因組學對83株來自香港社區居民,臨床病人以及食用動物產品(豬內臟)的產NDM大腸桿菌進行分析。除此之外,還收集了961株來自世界各地的NDM-E用於和香港菌株進行系統發育關係比較以及分析描述不同來源地區來源的NDM-E所攜帶的可動遺傳因子。IncX3 質粒在香港不同來源的NDM-E中最為普遍存在,但是人類與食用動物中的NDM-E存在於不同的的克隆型中以及攜帶不同的耐藥基因譜。氯霉素耐藥基因(floR, cmlA)在來自豬的菌株中明顯高於其他菌株但是ESBL基因攜帶率低。可動遺傳因子在不同地區分布不同,但是IncX3質粒是全球性威脅。IncB/O/K/Z質粒為中國農場和臨床病人中獨佔的一個類型。IncA/C2頻繁地在歐洲的菌株中存在但是在中國的菌株中沒有發現。ST48分支侷限於中國,包含從香港三種來源得到的以及在中國農場的肉禽,飼料和污水中分離到的NDM-E。總而言之,我們描述了香港不同來源CPE的分子流行病學特徵。並且基於WGS對CPE的系統發育關係,可動遺傳因子以及輔助基因譜進行了深入的研究。我們確定了在健康香港人群中EMRSA-15的出現和ESBL-E的高攜帶率,同時也發現CPE。產NDM的腸桿菌是香港各來源CPE中最常見的類型。IncX3介導的產NDM腸桿菌的傳播是香港以及全球最主要的CPE來源。ZHU, Chendi.Ph.D. Chinese University of Hong Kong 2021.Includes bibliographical references (leaves )Abstracts in English and Chinese.Title from PDF title page (viewed on ...
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