561 research outputs found
Collaboration Spotting for oral medicine.
No full textAIM:
The goal of the Collaboration Spotting project is to create an automatic system to collect information about publications and patents related to a given technology, to identify the key players involved, and to highlight collaborations and related technologies. The collected information can be visualized in a web browser as interactive graphical maps showing in an intuitive way the players and their collaborations (Sociogram) and the relations among the technologies (Technogram). We propose to use the system to study technologies related to oral medicine.
METHODS:
In order to create a sociogram, we create a logical filter based on a set of keywords related to the technology under study. This filter is used to extract a list of publications from the Web of ScienceTM database. The list is validated by an expert in the technology and sent to CERN where it is inserted in the Collaboration Spotting database. Here, an automatic software system uses the data to generate the final maps.
RESULTS:
We studied a set of recent technologies related to bone regeneration procedures of oro-maxillo-facial critical size defects, namely the use of porous hydroxyapatite (HA) as a bone substitute alone (bone graft) or as a tridimensional support (scaffold) for insemination and differentiation ex vivo of mesenchymal stem cells. We produced the sociograms for these technologies and the resulting maps are now accessible on-line.
CONCLUSION:
The Collaboration Spotting system allows the automatic creation of interactive maps to show the current and historical state of research on a specific technology. These maps are an ideal tool both for researchers who want to assess the state-of-the-art in a given technology, and for research organizations who want to evaluate their contribution to the technological development in a given field. We demonstrated that the system can be used in oral medicine as is produced the maps for an initial set of technologies in this field. We now plan to enlarge the set of mapped technologies in order to make the Collaboration Spotting system a useful reference tool for oral medicine research
Le management d'une unité d'élite -le RAID- entre 2013 et 2017 : Le rôle de la vision stratégique du chef et de sa prise en compte des émotions.: Le rôle de la vision stratégique du chef et de sa prise en compte des émotions.
No full textInternational audienceBecause for JM. Fauvergue (head of the RAID between 2013 and 2017) "the RAID is more comparable to the GIGN than it is to the BRI" this chapter explores the management of this elite unit of the national police during this period marked by a wave of terrorist attacks on French territory, unprecedented: Charlie Hebdo, Cash Casher Porte de Vincennes (Paris), the Promenade des Anglais (Nice), the Bataclan (Paris), Magnanville, Saint-Étienne du Rouvray... Based on a detailed monograph of the professional career of the head of the RAID during the period studied, the reflection highlights, doubly, the role of exercising transformational leadership, based on the notion of vision and the importance of taking emotions into account in managerial practice.Parce que pour JM. Fauvergue (chef du RAID entre 2013 et 2017) « le RAID est plus comparable au GIGN qu’il ne l’est à la BRI » ce chapitre explore le management de cette unité d’élite de la police nationale sur cette période marquée par une vague d’attentats terroristes sur le territoire français, sans précédent : Charlie Hebdo, Cash Casher porte de Vincennes (Paris), la promenade des anglais (Nice), le Bataclan (Paris), Magnanville, Saint-Étienne du Rouvray…Basée sur une monographie détaillée du parcours professionnel du patron du RAID sur la période étudiée, la réflexion met en avant, doublement, le rôle de l’exercice d’un leadership transformationnel, basé sur la notion de vision et l’importance de la prise en compte des émotions dans la pratique managériale
Exploring the effect of a second closely-timed PRP infiltration for tendinopathy
No full textpeer reviewedINTRODUCTION: Although PRP is very popular in sport, especially since it was removed from the doping list, it remains controversed in literature (3). Up to now, there exists no general agreement on the preparation and the use of PRP. Some clinical series have previously evaluated the effect of PRP in the treatment of proximal patellar tendinopathies. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of two or three successive infiltrations. The multiplication of infiltrations is arguably likely to increase the risks of complications, and this treatment can be expensive.
PURPOSE: We aimed to evaluate whether two infiltrations of PRP prove more effective than a single
treatment.
METHODS: Our study is a single blinded, randomized controlled clinical trial on leisure sportsmen with chronic proximal patellar tendinopathies, rebel to classical management, including physiotherapy, shock wave therapy… Twenty patients suffering from proximal patellar tendinopathies for over than 3 months were enrolled into the study. PRP was obtained using an aphaeresis machine (1). The subjects were split into two randomized groups and beneficed of 1 or 2 infiltrations of pure PRP, respectively. The one-year followup evaluation consisted of VAS, IKDC and VISAP scores, while algometer, isokinetic and ultrasounds evaluations were carried out up to 3 months.
RESULTS: The concentration of the PRP used for each infiltration was similar in both groups (913.20 ± 65.60 × 103/ L for group 1 and 917.90 ± 63.08for group 2, with virtually no red (<0.001 × 106/ L) nor white cells (<0.001 × 103/ L) in either group). The VAS significantly decreased with time over the 3 month followup period (p = 0002), with no difference observed between the two groups (p = 0.2). Values obtained with the pressure algometer increased with time across both groups over the 3 month followup period (p < 0.0001), and values were significantly higher for Group 1 (p = 0.001). The IKDC score increased with time in both groups over the followup period (p = 0.034), with values again significantly higher for Group 1 (p = 0.0026). The VISAP score increased with time in both groups over the followup period (p = 0.0023), with no difference observed between the groups (p = 0.41). No improvements in isokinetic physical performance were observed in either group. However, pain during E30 significantly decreased over the 3 month followup period (p = 0.027) for patients in both groups. No improvement in either jumping performances or in pain was observed in either group during optojump evaluation. No improvements in US findings were observed. However, an increase of the sagittal hypoechoic area was observed in Group1 (p = 0.0038). After one year, 90% patients of group 1 did not report anymore pain during daily activities, in comparison with only 20% in group 2. In group 1, 20% of subjects still described pain during work activities and 40% during practicing sports versus 40% and 70%, respectively, in group 2. One patient in each group did not return to sport; both subjects still experienced pain through daily and occupational work activities. Six subjects among the group 1 (67%) and 7 among the group 2 (78%) returned to their former sport, and 55% of both groups to the former level than before the tendinopathy. However, 44% of the group 1 and 78% of the group 2 still experienced pain during sports activities. The practiced sports were football, handball, cycling, running, fitness.On the other hand, patients with only few months of symptoms did not evolved more favorably than those with symptoms for longer.
CONCLUSIONS: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups after a followup period of 3 months. A second closely timed infiltration of PRP to treat proximal patellar tendinopathies is not necessary to improve the efficacy of this treatment in the short term (2). However, there remains a need to evaluate the longer term results.
REFERENCES:
Kaux JF, Croisier JL, Bruyere O, Rodriguez De La Cruz C, Forthomme B, Brabant G, Lapraille S, Lonneux V, Noel D, Le Goff C, Gothot A, Collette J, Crielaard JM. One injection of plateletrich
plasma associated to a submaximal eccentric protocol to treat chronic jumper's knee. J Sports Med Phys Fitness. 2015 Sep;55(9):95361
Kaux JF, Croisier JL, Forthomme B, Le Goff C, Buhler F, Savanier B, Delcour S, Gothot A, Crielaard JM. Using plateletrich plasma to treat jumper's knees: Exploring the effect of a second closelytimed
infiltration. J Sci Med Sport. 2016 Mar;19(3):2004.
Kaux JF, Drion P, Croisier JL, Crielaard JM. Tendinopathies and plateletrich plasma (PRP): from preclinical experiments to therapeutic use. J Stem Cells Regen Med. 2015 May 30;11(1):717. A1
Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein
No full textThe regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function
Identification and stage-specific association with the translational apparatus of TbZFP3, a CCCH protein that promotes trypanosome life-cycle development
No full textThe post-transcriptional control of gene expression is becoming increasingly important in the understanding of regulated events in eukaryotic cells. The parasitic kinetoplastids have a unique reliance on such processes, because their genome is organized into polycistronic transcription units in which adjacent genes are not coordinately regulated. Indeed, the number of RNA-binding proteins predicted to be encoded in the genome of kinetoplastids is unusually large, invoking the presence of unique RNA regulators dedicated to gene expression in these evolutionarily ancient organisms. Here, we report that a small CCCH zinc finger protein, TbZFP3, enhances development between life-cycle stages in Trypanosoma brucei. Moreover, we demonstrate that this protein interacts both with the translational machinery and with other small CCCH proteins previously implicated in trypanosome developmental control. Antibodies to this protein also co-immunoprecipitate EP procyclin mRNA and encode the major surface antigen of insect forms of T. brucei. Strikingly, although TbZFP3 is constitutively expressed, it exhibits developmentally regulated association with polyribosomes, and mutational analysis demonstrates that this association is essential for the expression of phenotype. TbZFP3 is therefore a novel regulator of developmental events in kinetoplastids that acts at the level of the post-transcriptional control of gene expression
Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea.
No full textBACKGROUND: A study was carried out in a village of the mainland region of Equatorial Guinea in order to ascertain a) which members of Anopheles gambiae complex could be involved in malaria transmission and b) the rate of infectivity for Anopheles melas comparing two different methods, a PCR able to detect sporozoite-DNA and an immunochromatographic assay MPR (Malaria Rapid Dipstick Panel Assay). METHODS: Mosquitoes were sampled at night by indoor captures in two houses of a coastal village in Equatorial Guinea (Ayantang). Collected mosquitoes were identified as An. gambiae s.l. These were individually dried into silica-gel. The head-thorax of the An. gambiae s.l. mosquitoes were analysed by PCR to verify that the species was of the gambiae complex. Individual head-thorax and pools (5 pools) of homogenized mosquitoes employed in Malaria Rapid Panel assay (MRP assay) were lysed and DNA was extracted. PCR was designed from the 753 base pair insert of pBRKl-14 and DNA was amplified. The relationship between dipstick and PCR to detect Plasmodium falciparum sporozoites was measured in terms of sensitivity, specificity and test association (Cohen's kappa value). RESULTS: Two hundred and sixty-four An. gambiae s.l. females were studied (214 individually and five pools with 10 mosquitoes in each). PCR analysis showed that 207 mosquitoes were An. melas, 3 An. gambiae s.s. and 4 could not be identified. By using PCR as the gold standard method when dipstick assay was compared, matching results were obtained for 6 mosquitoes and, in one case MRP was positive while PCR was not reactive. MRP assay showed a low sensitivity (3.3%) when compared with falciparum-DNA detection (17,7% and 14,3%, series A and B respectively). Agreement between the two test formats was low (kappa = 0,224). CONCLUSION: It was determined that An. melas is the main anopheline vector involved in malaria transmission in Ayantang, a coastal village in mainland Equatorial Guinea. A comparison of PCR and Vec-Test Assay, concluded that the PCR method proved to be a more sensitive and useful tool than the dipstick assay to determine the malarial infection rate in mosquitoes in an area of stable and high malaria transmission like Equatorial Guinea
A dimensão temporal do direito: um estudo a partir da teoria da sociedade moderna de Niklas Luhmann
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Florianópolis, 1997
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