1,582 research outputs found
Constitutional Jurisprudence, EU Law, and Italian Constitution
No full textThis entry deals with the case law of the Italian Constitutional Court on EU law and its relationship with the Italian Municipal law, including the Constitution. The gradual acceptance by the Consitutional Court of the principle of primacy of EU law, the confinement of such principle with the (mostly virtual) "counterlimits" doctrine, the openig of a direct dialogue with the Court of justice through the preliminary ruling mechanism have all changed dramaticaly the initial Court's approach to EU law. Still, one cannot say that the Constitutional Court case law is now completely settled. In some areas, the Court shows a "defensive" attitude against the Court of Justice. This is particularly the case of the recent rulings on the Charter of the Fundamental Rights of the European Union
Cysteine mediated multimerization of a recombinant dengue E fragment fused to the P64k protein following immobilized metal ion affinity chromatography
No full textA fragment of the Envelope protein of Dengue 2 virus encoding the amino acid (aa) 286-426 was fused to the P64k protein from Neisseria meningitidis. The hybrid gene (PD5) was expressed in Escherichia coli. The influence of using immobilized metal ion affinity chromatography (IMAC) in the purification process of PD5 was examined. After the elution, PD5 protein formed aggregates of high molecular weight depending on disulfide-bonds. The study of different conditions affecting the redox potential in the system revealed the influence of the copper ions on the multimerization of the protein, whereas metals with minor redox potential-for instance, zinc or nickel ions - did not cause this effect. It was also demonstrated that cysteines involved in this process belonged to the P64k protein. Finally, a PD5 purification process was established reaching a 85% of purity using the Zn2+ as metal ion in the IMAC. This is a very useful finding due to the wide use of P64k as a carrier protein and the advantages of the IMAC as a chromatographic process. © 2003 Elsevier Inc. All rights reserved
La muerte de un rojo : el asesinato de León Trotsky : la cobertura de El Universal / L. Hermida Román.
No full textTesis de Lic. en Periodismo, Escuela de Periodismo Carlos Septién GarcíaEn esta investigación periodística se presenta un análisis de lo publicado por el periódico El Universal acerca del asesinato de Trotsky (1940). Se analizan diez días consecutivos de cobertura periodística, desde el atentado hasta la muerte del ruso. Se revisan las omisiones y errores que cometió el diario en su labor informativa, y que no fueron aclarados o precisados. Y también se analiza el contexto político en el que se dan estas omisiones periodísticas
Comparative immunogenicity and protective capacity of two Dengue-4 vaccine candidates based on P64k-envelope domain III
No full textIn this work we compared, in the same immunization schedule in mice, two vaccine candidates against Dengue-4 based on the envelope protein domain III and the carrier P64k. Their molecular and antigenic features, immunogenicity and protective capacity in the viral encephalitis murine model are assessed. Our study endorses the strategy of using both sites of inclusion within the same P64k molecule, in order to enhance the immunogenicity of protein fragments
Fibrational induction rules for initial algebras
No full textThis paper provides an induction rule that can be used to prove properties of data structures whose types are inductive, i.e., are carriers of initial algebras of functors. Our results are semantic in nature and are inspired by Hermida and Jacobs’ elegant algebraic formulation of induction for polynomial data types. Our contribution is to derive, under slightly different assumptions, an induction rule that is generic over all inductive types, polynomial or not. Our induction rule is generic over the kinds of properties to be proved as well: like Hermida and Jacobs, we work in a general fibrational setting and so can accommodate very general notions of properties on inductive types rather than just those of particular syntactic forms. We establish the correctness of our generic induction rule by reducing induction to iteration. We show how our rule can be instantiated to give induction rules for the data types of rose trees, finite hereditary sets, and hyperfunctions. The former lies outside the scope of Hermida and Jacobs’ work because it is not polynomial; as far as we are aware, no induction rules have been known to exist for the latter two in a general fibrational framework. Our instantiation for hyperfunctions underscores the value of working in the general fibrational setting since this data type cannot be interpreted as a set
Planificación, seguimiento y enfoque de procesos, en la mejora de la UNC
No full textEl presente artículo analiza algunas aplicaciones de conceptos y recursos propios de la gestión de calidad, implementadas en distintas dependencias de la UNC. Entre los recursos utilizados en la experiencia, se cuentan la adopción de un enfoque al usuario, empleo sistemático de herramientas de planificación, énfasis en la capacitación e involucramiento, identificación y análisis de procesos, aplicación de AMFE (análisis de modos y efectos de falla) para el diseño de procesos operativos, elaboración y adopción de procedimientos y registros, seguimiento con indicadores y tratamiento con cartas de control estadístico, implementación de auditorias internas, tratamiento de no conformidades y aplicación de acciones correctivas y preventivas. La iniciativa fue impulsada por el área Recursos Humanos de la UNC, como parte de sus programas de capacitación. Este sistema de trabajo se aplicó de manera parcial en diversas entidades de la Universidad y de manera completa en la Biblioteca Mayor y en la Secretaría de Asuntos Académicos. Los sistemas desarrollados en las mismas fueron oportunamente certificados bajo Normas ISO 9001:2000. El artículo discute algunos de los recursos metodológicos utilizados, refleja diversas dificultades que debieron superarse y enumera los principales beneficios obtenidos
Endocrine therapy synergizes with smac mimetics to potentiate antigen presentation and tumor regression in hormone receptor-positive breast cancer
No full textHermida-Prado F., Xie Y., Sherman S., Nagy Z., Russo D., Akhshi T., Chu Z., Feit A., Campisi M., Chen M., Nardone A., Guarducci C., Lim K., Font-Tello A., Lee I., García-Pedrero J., Cañadas I., Agudo J., Huang Y., Sella T., Jin Q., Tayob N., Mittendorf E.A., Tolaney S.M., Qiu X., Long H., Symmans W.F., Jia-Ren L., Santagata S., Bedrosian I., Yardley D.A., Mayer I.A., Richardson E.T., Oliveira G., Wu C.J., Schuster E.F., Dowsett M., Welm A.L., Barbie D., Metzger O., Jeselsohn R
YES1 drives lung cancer growth and progression and predicts sensitivity to dasatinib
No full textGarmendia, I; Pajares, MJ; Hermida-Prado, F; Ajona, D; Bertolo, C; Sainz, C; Lavin, A; Remirez, AB; Valencia, K; Moreno, H; Ferrer, I; Behrens, C; Cuadrado, M; Paz-Ares, L; Bustelo, XR; Gil-Bazo, I; Alameda, D; Lecanda, F; Calvo, A; Felip, E; Sanchez-Cespedes, M; Wistuba, II; Granda-Diaz, R; Rodrigo, JP; Garcia-Pedrero, JM; Pio, R; Montuenga, LM; Agorreta, J
Influence of Culture Conditions on Poly-3-Hydroxybutyrate Production by a Newly Isolated Bacillus cereus Y23
No full textPolyhydroxyalkanoates (PHAs), environmental friendly polyesters, can be produced by a wide range of microorganisms in nutrient-limiting conditions. In the present study a new bacterial strain, able to produce high quantities of poly-3-hydroxybutyrate (PHB), is isolated from contaminated soil. The isolate is characterized and identified as Bacillus cereus Y23, based on morphological, physiological characteristics, and 16S ribosomal RNA gene sequences. PHB production is evaluated using different carbon and nitrogen sources and nutritional and culture conditions are optimized. The highest PHB production of 5.12 g L−1 was obtained in growth medium containing fructose and ammonium sulfate at a C/N ratio of 30, 0.3% yeast extract, 4.5 g L−1 phosphate solution, pH 7, inoculum size of 0.3%, and agitation rate of 150 rpm after 48 h of incubation at 32 °C. The PHB production decrement can be attributed to the intracellular utilization of the polymer during the stationary phase of the cellular growth and to the sporulation process. The presence of the main functional groups of PHB polymer is verified by Fourier-transform infrared analysis. At optimum conditions, B. cereus Y23 turned out to be a good candidate for industrial production of PHB since it accumulates up to 67.9% of its dry weight.Fil: Yashchuk, Oxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Hermida, Elida Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentin
A recombinant capsid protein from Dengue-2 induces protection in mice against homologous virus
No full textIn the present work, we study the immunogenicity and protective capacity of a recombinant capsid protein from Dengue-2 virus. The capsid gene was cloned under the T5 phage promoter and expressed in Escherichia coli. The recombinant protein was obtained mainly associated to the soluble fraction upon cellular disruption and exhibited a pattern of high aggregation, determined by gel filtration chromatography. The semipurified preparation was inoculated in mice and after three doses, no antiviral antibodies were induced. On the other hand, mice intracranially challenged with homologous lethal virus, exhibited statistically significant protection with respect to the control group. These results describe, for the first time, the protective capacity of the capsid protein of Dengue virus indicating the existence of a protector mechanism, which is totally independent of the antibodies. This lack of induction of antiviral antibodies makes the capsid protein an attractive vaccine candidate against dengue since eliminates the potential risk of the induction of antibody dependent enhancement associated to the current vaccines under study. © 2006 Elsevier Ltd. All rights reserved
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