1,720,971 research outputs found
Osteopontin Concentration in Prostates Fractions: A Novel Marker of Sperm Quality in Dogs
Full text linkThis study aimed to investigate the relationship between the sperm quality and the osteopontin (OPN) concentration in the prostates of Malakli shepherd dogs. Ejaculates were collected once by digital manipulation from 39 male dogs aged between 2 and 4 years and older. The first and third fractions of the ejaculate were centrifuged at 5000× g for 30 min, and supernatants were stored at −80 °C for further analysis of OPN using a double-antibody sandwich method (SEA899CA, Cloude-Clone Corp, Houston, TX, USA). Meanwhile, the second fractions were evaluated for sperm motility, concentration, viability, and rate of abnormal spermatozoa (head, acrosome, midpiece and tail abnormalities). The average concentration of OPN was 8.7 ± 5.2 ng/mL, and it differed significantly between the 1st 10.4 ± 5.3 ng/mL and 3rd 7.4 ± 5 ng/mL fractions. According to ROC (receiver operating characteristic curve) analysis, the OPN concentration had a better diagnostic ability for sperm motility (p p < 0.05). Additionally, the OPN concentration was negatively correlated with poor sperm morphology and motility. In conclusion, the OPN concentration in prostate-derived secretions may be a possible marker of sperm quality in dogs. Further research could explore the involvement of OPN in sperm motility during cryopreservation and in vivo fertility
The effects of silymarin in sodium fluoride-induced hepatotoxicity and oxidative stress in blood and tissue parameters in rats
No full textRatlarda Sodyum Florid İle Oluşturulan Hepatotoksite Ve Oksidatif Streste Silimarin'in Kan Ve Doku Parametreleri Üzerine Etkileri Flor oldukça reaktif bir element ve halojen ailesinin bir üyesidir. Bu çalışmada ratlarda florid kaynaklı hepatotoksisite ve oksidatif hasara karşı silimarin'in koruyucu etkisinin araştırılması amaçlandı. Ratlara bu amaçla, içme suyu ile 300 ppm NaF ad libitum verildi. Tedavi grubuna silimarin 200 ppm/gün gavaj ile verildi. Toplam 45 rat ile planlanan çalışma herbiri 9 rattan oluşan 5 grup (K, F, SF, S ve T) üzerinde gerçekleştirildi. Flor uygulanan grupta (F) karaciğer enzimlerinden AST aktivitesinin kontrol grubuna (K) göre istatistik önemde yüksek (p<0.001), ALT aktivitesinin ise istatistik önemde olmamakla beraber belirgin yüksek olması flor toksikasyonuna bağlı olarak doku yıkımını gösterdiği kanısına varıldı. Enzim aktivitelerinin flor ile birlikte silimarin uygulanan grupta (SF) ve 30 gün flor uygulanması sonrası 10 gün silimarin uygulanan grupta (T) flor uygulanan gruba (F) göre bariz düşük oluşu flor toksikasyonuna karşı silimarinin karaciğer koruyucu (hepatoprotektif) etkisi olarak değerlendirildi. Bu bulgu histopatolojik verilerle de açıkça desteklendiği görüldü. Flor uygulanan grupda (F) karaciğer dokusunda SOD ve GSH-Px değerlerindeki düşüş ile MDA düzeyindeki artış karaciğer dokusunda oksidatif hasarı gösterirken, silimarin ile birlikte flor uygulanan grupda (SF) ve 30 gün flor uygulamasını takiben 10 gün silimarin uygulanan tedavi grubunda (T) SOD ve GSH-Px düzeylerinin yüksek, MDA düzeylerinin düşük bulunması çalışmada kullanılan silimarinin antioksidan özelliği sebebiyle karaciğeri flor toksikasyonu sonucu gelişen oksidatif doku hasarına karşı korumuş olmasının bir göstergesi olarak yorumlandı. Karaciğer dokusunda ALT, AST, SOD ve GSH-Px gibi enzim aktivitelerinde ve lipid peroksidasyon belirteci oloan MDA gibi ürünlerin düzeylerinde saptanan değişiklikler ile western blot sonucu Bax, Kaspaz-3, Nox4, p38 MAPK, Hsp60 ve Hsp27 protein hatlarında gözlenen değişiklikler hem florun hepatotoksik ve oksidatif etkileri hem de silimarinin bu olumsuz etkilere karşı profilaktik ve terapötik etkileri olarak değerlendirildi. Flor uygulanan grupda (F) saptanan Nox 4, p 38 MAPK, Hsp 60 düzeylerindeki artış ve Hsp 27 düzeyindeki düşüş; oksidatif hasarı, apoptozisi ve mitokondrial stres nedenli olabilecek hücre hasarını gösterdiği kanısına varıldı. Silimarin'in özellikle hepatoprotektif etkisi göz önüne alınarak, ileri düzeyde planlanacak benzer florozis model çalışmalarında silimarinin sistemik etkisinin ve hücresel düzeyde sinyal iletim yollarının aydınlatılmasında yararlı olacağı düşünüldü.The Effects Of Silymarın In Sodium Fluorıde-Induced Hepatotoxıcıty And Oxıdatıve Stress In Rats Fluorine is a highly reactive element and a member of the halogen family. In this study, it was aimed to investigate the protective effect of silymarin against oxidative damage and oxidative damage in rats. For this purpose, 300 ppm NaF ad libitum was given to the rats with drinking water. The treatment group was given 200 mg / day gavage. A total of 45 rats were planned to be performed on 5 groups (K, F, SF, S and T) each consisting of 9 rats It was concluded that AST activity of liver enzymes in F group (F) was significantly higher than control group (K) (p <0.001) and ALT activity was statistically significant but not statistically significant, indicating tissue destruction due to fluorine toxicity. Enzymatic activity of the group with fluoride in the group with silymarin (SF) and 30 days after administration of fluoride 10 days in the group (s) applied to the group of fluoride (F) compared to the fluorine group (F) was considered to be the liver protective (hepatoprotective) effect of silimar against fluorine toxicity. This finding was clearly supported by histopathological data. In the F group (F), the decrease in SOD and GSH-Px levels in liver tissue and the increase in MDA levels showed oxidative damage in liver tissue, while in the fluorine treated group (SF) with silymarin (SF) and in the treatment group (T) in the treatment group (T) for 10 days following fluorine administration. and the high levels of GSH-Px and low levels of MDA were interpreted as an indicator of the fact that the silimarine used in the study protected the liver from oxidative tissue damage caused by fluoride toxicity due to its antioxidant properties. Changes in enzyme activity in liver tissue such as ALT, AST, SOD and GSH-Px and metabolite levels such as MDA, and changes in Bax, Caspase-3, Nox4, p38 MAPK, Hsp60 and Hsp27 protein lines as a result of western blot result in both hepatotoxic and oxidative effects of fluoride. de silimarine were evaluated as prophylactic and therapeutic effects against these adverse effects. Nox 4, p 38 MAPK, Hsp 60 levels and decrease in Hsp 27 level detected in F group applied in fluorine group (F); It was concluded that it shows cell damage caused by oxidative damage, apoptosis and mitochondrial stress. Considering the hepatoprotective effect of silymarin, it was thought that the systemic effect of silimarine and similar signal transduction pathways at the cellular level could be helpful in the study of similar fluorosis model
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Ankara bölgesinden toplanan bal arısı kovanı ürünlerinin antioksidan özelliklerinin ve toplam fenolik ve flavonoid içeriklerinin değerlendirilmesi
Full text linkIn this study, the total phenolic (TP) and total flavonoid (TF) profiles of multifloral honey, bee bread, bee pollen, and drone larvae (apilarnil), which are among the products of bee hives, were determined. In addition, the antioxidant activities of the aforementioned products were determined by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP) assay, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay methods. Honey, bee bread, bee pollen, and apilarnil samples collected from 10 honey bee hives in the Ankara region were the material of the study. As a result of the analysis, TP content levels were found to be in the order of bee pollen > bee bread > honey > apilarnil, while TF contents were in the order of bee pollen > bee bread > apilarnil > honey. Considering the results of the DPPH, FRAP, and ABTS assays, the levels of activity were determined in the order of honey > bee pollen > bee bread > apilarnil. The highest antioxidant activity level determined in honey was concluded to be the result of synergistic antioxidant effects of other bioactive complex substances contained in honey. Therefore, we believe that bioactive complex substances that increase the antioxidant activity level of honey should be evaluated in future studies.Bu çalışmada, arı ürünleri olan multifloral bal, arı ekmeği, arı poleni ve erkek arı larvası (apilarnil)'in toplam fenolik (TP) ve toplam flavonoid (TF) profilleri ortaya konuldu. Bunun yanı sıra söz konusu örneklerin antioksidan aktiviteleri, 2,2′-azinobis (3-ethylbenzothiazolin)-6-sulfphonate (ABTS) testi ve ferric reducing antioxidant power (FRAP) testi ve 1,1-diphenyl-2-picrylhydrazy (DPPH) testi metotları kullanılarak belirlendi. Çalışmada materyalini Ankara yöresindeki 10 bal arısı kovanından toplanan bal, arı ekmeği, arı poleni ve apilarnil örnekleri oluşturdu. Yapılan analizlerin sonucunda bal arısı ürünlerinin toplam fenolik içerik düzeyine göre sıralaması arı poleni > arı ekmeği > bal > apilarnil olarak bulunurken, bal arısı ürünlerinin toplam flavonoid içeriği düzeyine göre sıralaması ise şu şekildedir: arı poleni > arı ekmeği > apilarnil > bal. Bal arısı kovan ürünlerinin DPPH, FRAP ve ABTS analizine göre sıralaması bal > arı poleni > arı ekmeği > apilarnil olarak belirlendi. Sonuç olarak balın en yüksek antioksidan aktivite düzeyi balın içerdiği diğer biyoaktif kompleks maddelerin sinerjistik antioksidan etkileri olarak değerlendirilmiştir ve bu nedenle balın antioksidan aktivite düzeyini artıran biyoaktif kompleks maddelerin ileriki çalışmalarda değerlendirilmesi gerektiği kanaatindeyiz
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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