19 research outputs found
Structures, Mechanisms and Biogenesis of Biliproteins
No full textBilins are biological pigments that play important roles in many light-dependent processes in both photosynthetic and non-photosynthetic organisms. Despite the extensive studies in recent decades, many important questions regarding the light signaling mechanisms of bilin-based photoreceptors and biogenesis of light harvesting phycobiliproteins remain unanswered.
Reversible protein phosphorylation is the most widespread regulatory mechanism in signal transduction. In response to light, bacteriophytochromes undergo autophosphorylation at the histidine kinase (HK) domain, as the first step of the predominant two-component signal-transduction mechanism in bacteria. However, the molecular basis and directionality of the autophosphorylation process (cis; intra-subunit or trans; inter-subunit) within the dimeric HK remain unknown. In this dissertation we present a complementary rescue strategy based on loss-of-function mutants of conserved His and catalytic Asn residues in the HK domain of two tandem bacteriophytochromes from the photosynthetic bacterium Rhodopseudomonas palustris RpBphP2 and RpBphP3 to distinguish the mode of phosphorylation between cis and trans by HK assay using phos-tag gels. Our results unambiguously show that these bacteriophytochromes undergo trans phosphorylation individually and as two tandem heterodimers. The crystal structure of the isolated HK domain of the full-length RpBphP2 and the modelled full-length structure allowed to understand the catalytic mechanism of the HK trans-autophosphorylation in bacteriophytochromes.
Extensive bilin pigment diversity of the light harvesting complexes in marine Synechococcus significantly contributes to their widespread abundance in the ocean. Such diversity depends on bilin lyases that attach chemically distinct chromophores to the phycobiliproteins. A distinct group of the E/F type bilin lyases acquired additional abilities to isomerize bilins, which further expanded the pigment repertoire for light absorption. Despite recent structural studies on a representative CpcE/F, the overall architecture, active site geometry and reaction mechanism remain unresolved for the E/F family of bilin lyases. In this dissertation we report the crystal structure at 2.5 Å resolution of MpeQ, a newly identified bilin lyase-isomerase that plays important roles in type IV chromatic acclimation. The single-chain -solenoid structure of MpeQ reveals a large active-site chamber in a “question-mark”-shaped protein architecture. Using site-directed mutagenesis, we identified key residues responsible for the lyase and isomerase activities of MpeQ. Our structural analyses have revealed a nucleophilic tyrosine within a catalytic triad in several bilin lyases, suggesting a shared reaction mechanism among bilin lyases of distinct protein scaffolds. We also propose that the isomerase activity of MpeQ is resulted from steric incompatibility between the active site geometry and the A-ring conformation of the bilin substrate
The organization and operation of Pre- Hospital care emergency ambulance Service (Suwaseriya1990) in Sri Lanka
No full textAbstract: In 2016 Suwaseriya1990 ambulance service was launched and in 2018, Suwaseriya1990 Foundation was established by parliamentary act No.18 of 2018 to deliver high quality pre-hospital emergency health care to people of Sri Lanka. At present, Suwaseriya1990 provide island-wide, pre- hospital emergency care to all Sri Lankan free of charge at point of delivery. Objective of this study is to study the organization and operation of Pre-Hospital Care emergency ambulance service (Suwaseriya1990) in Sri Lanka
A desk review was conducted to studying the organization and operation of Suwaseriya 1990 and thematic areas were based on World Health Organization’s Health system frame work. Narrative data synthesis was done for reporting.
Thematic areas were recorded positives in leadership and governance, services delivery, information technology and work force. Gaps and opportunities for development can be seen in leadership and governance, work force and service delivery. Average response time of ambulance is less than 14.32 minute in non-Colombo area and 8.38 minute in Colombo area. Some policies like equity, quality and risk reduction and mechanism for evidence base practice is lacking. Opportunities are existed for service expansion and staff development
Improvement of Capacity of Emergency Medical Technician and strengthening of evidence base practices are recommended for further improvement of services. Further policy development of equity, quality and risk reduction is recommended
Keywords: Emergency Medical Care, Suwaseriya1990, Pre-Hospital Care, Emergency ambulance service, Emergency Medical Technician, Call center.
Title: The organization and operation of Pre- Hospital care emergency ambulance Service (Suwaseriya1990) in Sri Lanka
Author: Indika Wanninayake, Hemantha Ranasinghe
International Journal of Recent Research in Social Sciences and Humanities (IJRRSSH)
ISSN 2349-7831
Vol. 9, Issue 2, April 2022 - June 2022
Page No: 297-302
Paper Publications
Website: www.paperpublications.org
Published Date: 28-June-2022
DOI: https://doi.org/10.5281/zenodo.6773038
Paper download Link (Source)
https://www.paperpublications.org/upload/book/The%20organization%20and%20operation-28062022-6.pdfInternational Journal of Recent Research in Social Sciences and Humanities (IJRRSSH), ISSN 2349-7831, Paper Publications, Website: www.paperpublications.or
Reasons for including multiage settings in teacher preparation
No full textTeaching is a complex and cognitively demanding exercise where teachers need to draw on their expertise in a contextually-sensitive manner. Multiage settings that comprise students with widely different abilities, ages, and cognitive and linguistics capabilities, require teachers to develop and upgrade specific pedagogic and administrative skills. Teacher education programmes offered at Australian universities however are yet to fully recognise this need. In this paper, the author develops an argument for inclusion of specific training in multiage
education in Australian teacher education programmes
Dimer asymmetry and light activation mechanism in brucella blue-light sensor histidine kinase
No full textThe ability to sense and respond to environmental cues is essential for adaptation and survival in living organisms. In bacteria, this process is accomplished by multidomain sensor histidine kinases that undergo autophosphorylation in response to specific stimuli, thereby triggering downstream signaling cascades. However, the molecular mechanism of allosteric activation is not fully understood in these important sensor proteins. Here, we report the full-length crystal structure of a blue light photoreceptor LOV histidine kinase (LOV-HK) involved in light-dependent virulence modulation in the pathogenic bacterium Brucella abortus. Joint analyses of dark and light structures determined in different signaling states have shown that LOV-HK transitions from a symmetric dark structure to a highly asymmetric light state. The initial local and subtle structural signal originated in the chromophore-binding LOV domain alters the dimer asymmetry via a coiled-coil rotary switch and helical bending in the helical spine. These amplified structural changes result in enhanced conformational flexibility and large-scale rearrangements that facilitate the phosphoryl transfer reaction in the HK domain. IMPORTANCE Bacteria employ two-component systems (TCSs) to sense and respond to changes in their surroundings. At the core of the TCS signaling pathway is the multidomain sensor histidine kinase, where the enzymatic activity of its output domain is allosterically controlled by the input signal perceived by the sensor domain. Here, we examine the structures and dynamics of a naturally occurring light-sensitive histidine kinase from the pathogen Brucella abortus in both its full-length and its truncated constructs. Direct comparisons between the structures captured in different signaling states have revealed concerted protein motions in an asymmetric dimer framework in response to light. Findings of this work provide mechanistic insights into modular sensory proteins that share a similar modular architecture.Fil: Rinaldi, Jimena Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Fernandez, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Shin, Heewhan. University of Illinois; Estados UnidosFil: Sycz, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Gunawardana, Semini. University of Illinois; Estados UnidosFil: Kumarapperuma, Indika. University of Illinois; Estados UnidosFil: Aragón Paz, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Otero, Lisandro Horacio. Plataforma Argentina de Biología Estructural y Metabolómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Cerutti, Maria Laura. Plataforma Argentina de Biología Estructural y Metabolómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Zorreguieta, Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ren, Zhong. University of Illinois; Estados UnidosFil: Klinke, Sebastian. Plataforma Argentina de Biología Estructural y Metabolómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Yang, Xiaojing. University of Illinois; Estados UnidosFil: Goldbaum, Fernando Alberto. Plataforma Argentina de Biología Estructural y Metabolómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin
ANALISIS PERTIMBANGAN HUKUM HAKIM DALAM MENJATUHKAN PIDANA MATI BAGI TERDAKWA KEKERASAN SEKSUAL TERHADAP ANAK (Studi Kasus Putusan Nomor : 86/Pid.SUS/2022/PT BDG)
No full textThis study aims to find out and analyse the judge's legal considerations in imposing the death penalty for defendants of sexual violence against children Decision Number: 86 / Pid.SUS / 2022 / PT BDG regarding the imposition of
criminal verdicts is related to the fulfilment of the principle of restorative justice for victims of sexual violence, where in the decision of the Bandung District Court Judges according to the author has not fulfilled restorative justice for victims so that the Public Prosecutor submits to the level of Appeal with the aim of asking for legal justice against a verdict that is considered inappropriate in imposing criminal sanctions on the victim who is a teacher who committed sexual violence against the victim who is a student of his own number of 12 people who are on average underage
ANALISIS PEMILIHAN KEPALA DESA DI DESA TALONTAM BENAI KECAMATAN BENAI KABUPATEN KUANTAN SINGINGI TAHUN 2019-2025
No full textThis research was carried out in Talontam Benai Village, Benai District, Kuantan Singingi Regency. The aim of this research is to find out how the village head is elected in Talontam Benai Village, Benai District, Kuantan Singingi Regency in 2019-2025. The formulation of the problem in the research is How to Selection of Village Heads in Talontam Benai Village, Benai District, Kuantan Singingi Regency in 2019-2025. The sampling technique uses the Simple Random Sampling method, namely taking sample members from the population randomly without paying attention to the strata in the population, where the number of samples for this research was 42 people. The data analysis used is quantitative descriptive, namely analyzing data obtained from the results of distributing questionnaires, then processing it, and then the author draws conclusions from the results of processing the data. The results of the questionnaire distribution show that the Election of Village Heads in Talontam Benai Village, Benai District, Kuantan Singingi Regency in 2019-2025 produced a total average score of 125, meaning it was in the quite good interval, as well as observations in the field which showed that the Election of Village Heads in Talontam Benai Village was quite good. Benai District, Kuantan Singingi Regency, 2019-2025, with all the indicators tested, namely the Preparation Stage for Village Head Election, Village Head Nomination Stage, Voting Stage, Overall Determination is Good Enough
Crystal-on-crystal chips for in situ serial diffraction at room temperature
No full textRecent developments in serial crystallography at X-ray free electron lasers (XFELs) and synchrotrons have been driven by two scientific goals in structural biology - first, static structure determination from nano or microcrystals of membrane proteins and large complexes that are difficult for conventional cryocrystallography, and second, direct observations of transient structural species in biochemical reactions at near atomic resolution. Since room-temperature diffraction experiments naturally demand a large quantity of purified protein, sample economy is critically important for all steps of serial crystallography from crystallization, crystal delivery to data collection. Here we report the development and applications of "crystal-on-crystal" devices to facilitate large-scale in situ serial diffraction experiments on protein crystals of all sizes - large, small or microscopic. We show that the monocrystalline quartz as a substrate material prevents vapor loss during crystallization and significantly reduces background X-ray scattering. These devices can be readily adopted at XFEL and synchrotron beamlines, which enable efficient delivery of hundreds to millions of crystals to the X-ray beam, with an overall protein consumption per dataset comparable to that of cryocrystallography
Penerapan pelaporan sosial pada perusahaan sektor energi yang sudah terdaftar di Bursa Efek Indonesia
No full textThis study aims to analyze the company\u27s Corporate Social Responsibility disclosures presented in the Sustainability Report based on the latest reporting standards issued by the Global Reporting Initiative, namely the GRI Standards, and to find out the implementation of the Corporate Social Responsibility (CSR) Report conducted by PT Elnusa Tbk, PT Medco Energy Internasional. Tbk, PT Perusahaan Gas Negara Tbk, PT Adaro Energy Tbk. PT AKR Corporindo Tbk, PT Indika Energy Tbk, PT Energi Mega Persada Tbk, PT Bumi Resources Tbk, PT Super Energy Tbk, and PT Ratu Prabu Energy Tbk. This research uses descriptive qualitative method. Descriptive qualitative is used in this study where the author describes the object of research according to the actual situation by collecting what is needed and related to interpret and compare the results of his analysis of energy sector companies for conclusions. The conclusion is that by using the GRI standards for CSR reporting, the report not only informs the process of implementing CSR, but also informs the wider community about the company\u27s profile and strategy, as well as the management approach taken and other economic benefits
Impact of the costumes on the theatrical style, in a holistic sensorial platform
No full textThe purpose of this study is to find out how and in what ways do costumes impact on the theatrical style in the play, in a holistic sensorial platform. The research is done based on the Comprehensive Design project done by the author, which was ‘Designing Costumes for an Experimental Play’. The play “The Irresistible Rise of Mr. Signno” is an experimental theatre practice done by the theatre practitioner Indika Ferdinando. Following practice based research methodology, this research will discuss the impact of costumes on the theatrical style by designing costumes for the above mentioned experimental theatre practice. In the play the director’s aim is to bring back the concept of holism in ritual theater, into the contemporary theatre. Upon completion of the research, it was found out that costumes are a key player, and it has and strong impact on the theater style in a holistic platform. The research was made possible by a Senate Research Committee Grant, University of Moratuwa, Sri Lanka under the grant number SRC/ST/2017/16
Crystal structure and molecular mechanism of an E/F type bilin lyase-isomerase
No full textInternational audienceChromophore attachment of the light-harvesting apparatus represents one of the most important post-translational modifications in photosynthetic cyanobacteria. Extensive pigment diversity of cyanobacteria critically depends on bilin lyases that covalently attach chemically distinct chromophores to phycobiliproteins. However, how bilin lyases catalyze bilin ligation reactions and how some lyases acquire additional isomerase abilities remain elusive at the molecular level. Here, we report the crystal structure of a representative bilin lyase-isomerase MpeQ. This structure has revealed a “question-mark” protein architecture that unambiguously establishes the active site conserved among the E/F-type bilin lyases. Based on structural, mutational, and modeling data, we demonstrate that stereoselectivity of the active site plays a critical role in conferring the isomerase activity of MpeQ. We further advance a tyrosine-mediated reaction scheme unifying different types of bilin lyases. These results suggest that lyases and isomerase actions of bilin lyases arise from two coupled molecular events of distinct origi
