110 research outputs found
A comprehensive analysis of Trehalose-6-phosphate synthase (TPS) gene for salinity tolerance in chickpea (Cicer arietinum L.)
No full textSoil salinity affects various crop cultivation but legumes are the most sensitive to salinity. Osmotic stress is the first stage of salinity stress caused by excess salts in the soil on plants which adversely affects the growth instantly. The Trehalose-6-phosphate synthase (TPS) genes play a key role in the regulation of abiotic stresses resistance from the high expression of different isoform. Selected genotypes were evaluated to estimate for salt tolerance as well as genetic variability at morphological and molecular level. Allelic variations were identified in some of the selected genotypes for the TPS gene. A comprehensive analysis of the TPS gene from selected genotypes was conducted. Presence of significant genetic variability among the genotypes was found for salinity tolerance. This is the first report of allelic variation of TPS gene from chickpea and results indicates that the SNPs present in these conserved regions may contribute largely to functional distinction. The nucleotide sequence analysis suggests that the TPS gene sequences were found to be conserved among the genotypes. Some selected genotypes were evaluated to estimate for salt tolerance as well as for comparative analysis of physiological, molecular and allelic variability for salt responsive gene Trehalose-6-Phosphate Synthase through sequence similarity. Allelic variations were identified in some selected genotypes for the TPS gene. It is found that Pusa362, Pusa1103, and IG5856 are the most salt-tolerant lines and the results indicates that the identified genotypes can be used as a reliable donor for the chickpea improvement programs for salinity tolerance
Evaluation and Comparison of the Surface Roughness and Porosity of Different Provisional Restorative Materials: An in vitro Study
No full textABSTRACT
Aim
The purpose of this study was to evaluate and compare the surface roughness and porosity of different provisional restorative materials.
Materials and methods
Provisional restorative materials were divided into following three groups: Dental products of india (DPI), Protemp, Tempofit. For each group, wax block with 20 × 10 × 3 mm was made for making vinyl polysiloxane impression material to give 12 samples in each group of three different provisional restorative materials. The acrylic resin was inserted into the silicon impression mold. A total of 12 specimens of one material were obtained. The specimens were finished with the help of lathe using a sequence of grit sand paper. The surface roughness was verified with the help of a micron dial indicator. To facilitate the porosity readings, the specimens were immersed in dye for 2 hours. The number of pores in each area was determined with a stereomicroscope with magnification 1× 50× to check the porosity of three different provisional restorative materials. Values were subjected to statistical analysis.
Results
Analysis of variance (ANOVA) test was used to compare between Tempofit, Protemp, and DPI. The results obtained indicated that surface roughness of Protemp was least compared with Tempofit and DPI. The ANOVA test was used to check surface area of porosities in each provisional material, followed by Kruskal–Wallis test and Mann–Whitney test (highly significant) (p < 0.001). The results obtained indicate that Protemp material showed the least number of porosities and minimal surface roughness followed by Tempofit and DPI. Henceforth, it can be concluded that among the three tested materials, Protemp was the best material which can be used for provisional restorations.
Conclusion
Surface roughness and porosity were compared among Protemp, Tempofit, and DPI material; the best results were obtained with the use of Protemp material which had shown the least number of porosities and minimal surface roughness.
How to cite this article
Kumar GV, Devi R, Anto N. Evaluation and Comparison of the Surface Roughness and Porosity of Different Provisional Restorative Materials: An in vitro Study. CODS J Dent 2016;8(1):39-45.
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Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat
No full textGenomics and genome editing promise enormous opportunities for crop improvement and elementary research. Precise modification in the specific targeted location of a genome has profited over the unplanned insertional events which are generally accomplished employing unadventurous means of genetic modifications. The advent of new genome editing procedures viz; zinc finger nucleases (ZFNs), homing endonucleases, transcription activator like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enable molecular scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, all these techniques are exorbitant and tedious since their prerequisites are difficult processes that necessitate protein engineering. Contrary to first generation genome modifying methods, CRISPR/ Cas9 is simple to construct, and clones can hypothetically target several locations in the genome with different guide RNAs. Following the model of the application in crop with the help of the CRISPR/Cas9 module, various customized Cas9 cassettes have been cast off to advance mark discrimination and diminish random cuts. The present study discusses the progression in genome editing apparatuses, and their applications in chickpea crop development, scientific limitations, and future perspectives for biofortifying cytokinin dehydrogenase, nitrate reductase, superoxide dismutase to induce drought resistance, heat tolerance and higher yield in chickpea to encounter global climate change, hunger and nutritional threats
भारत में मत्स्य सुरक्षा के लिए मांग और आपूर्ति के मानदंड
No full textभारत में मत्स्य सुरक्षा के लिए मांग और आपूर्ति के मानदं
A CONCEPTUAL APPRAISAL OF VIRECHANA KARMA
No full textPanchakarma is getting global attention nowadays and Virechana is one among its procedures which is actively used by the Ayurvedic fraternity. Four different types of Virechana namely Anulomana, Sramsana, Bhedana and Rechana are mentioned in our classics but they are not apprised properly for their clinical utility. The Brihattrayis give ample illustrations of their use without highlighting the underlying rationale. Sarangdhara Samhita defines each of this therapeutics without indicating the disease conditions. This paper tries to understand the specific clinical utility of the four types of Rechana by tracing back their indications in scriptures. Anulomana is a mild form of Virechana where the Paka of Malas occur. Sramsana is a mild form of Virechana which does not cause Paka of Malas. Bhedana does not cause Paka of Malas but it is a strong type of Virechana. Rechana also does not cause Paka of mala but the strength of the procedure can be modulated by the use of different types of drugs. The selection of the type of Rechana depends on the pharmaco-therapeutic action necessary to harmonise the Dosa, Dhatu and Mala without causing any further complication or Upadrava
Smokestack leak on indocyanine green angiography in acute central serous chorioretinopathy
No full text"Chitraka (Plumbago Zeylanica Linn.): Exploring The Realms Of Rasayana (Rejuvenation) Potential Of Ayurveda
No full textThe present review explores the possible Rasayana properties of Chitraka, the dried mature root of Plumbago zeylanica Linn., within the framework of Ayurveda. Rasayana chikitsa, an integral component of Ayurvedic medicine, focuses on rejuvenation to achieve optimal physical, mental, and spiritual health. Chitraka, identified as both Kamya Rasayana and Naimittika Rasayana in classical Ayurveda, has been traditionally lauded for its potential in prolonging life and treating specific ailments.
The classical appraisal of Chitraka\u27s Rasayana effect, as per Ashtanga Hridaya, aligns with its historical association with enhancing life span and managing haemorrhoids (Arsas), neurodegenerative diseases (vata vyadhi) and skin diseases (Kushta and switra). Recent research delves into the diverse therapeutic potentials of Chitraka, revealing its anti-hemorrhoidal, anti-inflammatory, anti-arthritic, anti-microbial, wound-healing, anti-androgenic alopecia, antioxidant, hepatoprotective, nephroprotective, and anti-tumor effects.
This exploration of Chitraka\u27s multifaceted therapeutic potential bridges ancient wisdom with modern scientific scrutiny, showcasing its relevance in addressing a spectrum of health challenges. The review emphasizes the need for continued research to unlock Chitraka\u27s full potential, contributing to the evolving landscape of holistic health practices and fostering the integration of Ayurvedic principles into mainstream healthcare
Management of bipolar depression with Lamotrigine: an antiepileptic mood stabilizer
No full textThe efficacy of lamotrigine in the treatment of focal epilepsies have already been reported in several case reports and open studies, which is thought to act by inhibiting glutamate release through voltage-sensitive sodium channels blockade and neuronal membrane stabilization. However, recent findings have also illustrated the importance of lamotrigine in alleviating the depressive symptoms of bipolar disorder, without causing mood destabilization or precipitating mania. Currently, no mood stabilizers are available having equal efficacy in the treatment of both mania and depression, two of which forms the extreme sides of the bipolar disorder. Lamotrigine, a well established anticonvulsant has received regulatory approval for the treatment and prevention of bipolar depression in more than 30 countries worldwide. Lamotrigine, acts through several molecular targets and overcomes the major limitation of other conventional antidepressants by stabilizing mood from ‘below baseline’ thereby preventing switches to mania or episode acceleration, thus being effective for bipolar I disorder. Recent studies have also suggested that these observations could also be extended to patients with bipolar II disorder. Thus, lamotrigine may supposedly fulfill the unmet requirement for an effective depression mood stabilizer
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