15 research outputs found
A Tradução como um ‘Acordo Dialógico’: Uma Perspectiva Bakhtiniana
No full textTRADUÇÃO PARA LIBRAS: José Ednilson Gomes de Souza Júnior (Supervisão Geral), Daniela Bieleski e Lais dos Santos di Benedetto Frasca (Orientação e Revisão Final), Raphael Freire Marques (Coordenador do Projeto), Crystonberg da Silva Souza, Julian Ramalho Henry, Marcos Antônio da Silva Filho, Maria Zenaide Pereira Rodrigues, Samantha Alves da Silva (Tradutores), Felipe Lima Souza e Nicolle Campos (Revisores
Ecocritical study of Kiran Desai’s Hullabaloo in the Guava Orchard
No full textAbstract
Bhartrhari and Bakhtin not only belong to two different philosophical traditions of language but they are also divided by a huge time gap of more than 1300 years. While Bakhtin’s philosophy has been termed as dialogism as it puts forth the significance of “both/and” in meaning construction and identity formation, Bhartrhari is seen as a monist and a universalist advocating sabda-tattva as the underlying principle for meaning construction. Bakhtin foregrounds dialogue as the basis of language and human existence and demonstrates how dialogic meaning is unfinalizable as it moves into the realm of the outer and the unknown. Whereas for Bhartrhari, meaning is obtained due to the interplay between vacaka and vacya, i.e., ‘whatever can be expressed in words and the words which express them’ (Iyer 1969: 147). Bakhtin stays away from metaphysics and explains the phenomenal world by identifying the various speech genres that are used in communication. On the contrary, Bhartrhari emphasizes the inextricable link between the phenomenal and the transcendental. Metaphysics and mysticism are very integral to Bhartrahri’s theoretical formulations
‘Vakyasphota’ and ‘Dialogic Dispersion’: Interlinking Bhartrhari and Bakhtin
No full textAbstract
Bhartrhari and Bakhtin not only belong to two different philosophical traditions of language but they are also divided by a huge time gap of more than 1300 years. While Bakhtin’s philosophy has been termed as dialogism as it puts forth the significance of “both/and” in meaning construction and identity formation, Bhartrhari is seen as a monist and a universalist advocating sabda-tattva as the underlying principle for meaning construction. Bakhtin foregrounds dialogue as the basis of language and human existence and demonstrates how dialogic meaning is unfinalizable as it moves into the realm of the outer and the unknown. Whereas for Bhartrhari, meaning is obtained due to the interplay between vacaka and vacya, i.e., ‘whatever can be expressed in words and the words which express them’ (Iyer 1969: 147). Bakhtin stays away from metaphysics and explains the phenomenal world by identifying the various speech genres that are used in communication. On the contrary, Bhartrhari emphasizes the inextricable link between the phenomenal and the transcendental. Metaphysics and mysticism are very integral to Bhartrahri’s theoretical formulations
Real-Time Hashtag based Event Detection Model with Sentiment Analysis for Recommending user Tweets
No full textMRR and TWR evaluation on electrical discharge machining of Ti-6Al-4V using tungsten : copper composite electrode
No full textIdentification of Rays through DNA Barcoding: An Application for Ecologists
No full textDNA barcoding potentially offers scientists who are not expert taxonomists a powerful tool to support the accuracy of field studies involving taxa that are diverse and difficult to identify. The taxonomy of rays has received reasonable attention in Australia, although the fauna in remote locations such as Ningaloo Reef, Western Australia is poorly studied and the identification of some species in the field is problematic. Here, we report an application of DNA-barcoding to the identification of 16 species (from 10 genera) of tropical rays as part of an ecological study. Analysis of the dataset combined across all samples grouped sequences into clearly defined operational taxonomic units, with two conspicuous exceptions: the Neotrygon kuhlii species complex and the Aetobatus species complex. In the field, the group that presented the most difficulties for identification was the spotted whiptail rays, referred to as the 'uarnak' complex. Two sets of problems limited the successful application of DNA barcoding: (1) the presence of cryptic species, species complexes with unresolved taxonomic status and intra-specific geographical variation, and (2) insufficient numbers of entries in online databases that have been verified taxonomically, and the presence of lodged sequences in databases with inconsistent names. Nevertheless, we demonstrate the potential of the DNA barcoding approach to confirm field identifications and to highlight species complexes where taxonomic uncertainty might confound ecological data
Estudo da atividade antitumoral do extrato bruto e frações de Casearia sylvestris
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica, Florianópolis, 2010O desenvolvimento de novas alternativas terapêuticas, como as terapias que utilizam as plantas medicinais, tem despertado grande interesse no tratamento do câncer, pois as terapias disponíveis não são capazes de regredir totalmente a evolução da patologia e/ou apresentam elevada toxicidade. A C. sylvestris, planta medicinal que possui vários usos populares no Brasil e outros paises americanos, tem sido indicada para o tratamento de tumores. Nesse contexto, o presente trabalho propôs avaliar as atividades citotóxica, antiproliferativa, pró-apoptótica, antiangiogênica e antitumoral do extrato bruto (EB-ETOH), e das frações metanólica (f-MeOH), acetato de etila (f-AcOEt) e clorofórmica (f-CHCl3) de Casearia sylvestris utilizando modelos experimentais in vitro e in vivo. Nos ensaios do MTT e incorporação de timidina triciada realizados respectivamente, para investigar a citotoxicidade e influência dos extratos sobre a proliferação celular, os resultados demonstraram que EB-ETOH, f-CHCl3 e f-AcOEt reduziram de maneira significativa a viabilidade e proliferação de células do carcinoma de Ehrlich, sendo a citotoxicidade promovida pelo tratamento com esses extratos, tempo e concentração dependente. Diante do efeito citotóxico promovido por EB-ETOH, f-CHCl3 e f-AcOEt sobre células do carcinoma de Ehrlich, avaliou-se o tipo de morte celular promovida pelos tratamentos. A coloração com brometo de etídeo/laranja de acridina (BE/LA) revelou que o provável tipo de morte celular induzida pelos tratamentos trata-se de apoptose, uma vez que a grande maioria das células, após terem entrado em contato com a referida solução corante, adquiriram uma coloração laranja-avermelhada, característica da ocorrência do fenômeno apoptótico. Entretanto, em relação à avaliação da atividade nucleásica, verificada através da realização do teste de dano ao DNA plasmidial, observou-se que os efeitos antiproliferativo e citotóxico induzidos por EB-ETOH, f-CHCl3 e f-AcOEt, não são mediados por uma interação e dano direto ao DNA. Além disso, a realização desse ensaio permitiu observar que os extratos têm a capacidade de proteger o DNA do ataque induzido por EROs, excluindo-se portanto, a possibilidade de estarem exercendo seus efeitos citotóxico e antiproliferativo através da indução de estresse oxidativo no núcleo. As avaliações morfológicas e bioquímicas realizadas nas amostras obtidas de camundongos portadores do tumor ascítico de Ehrlich mostraram que os animais tratados com EB-ETOH e f-CHCl3 apresentaram menor ganho de peso corporal (26 e 31%, respectivamente) e de circunferência abdominal (52 e 58%, respectivamente), menor volume de líquido ascítico (46 e 43%, respectivamente), aumento do percentual de longevidade (48 e 40%, respectivamente) e da proporção de células inviáveis/viáveis (70 e 78%, respectivamente), quando comparados ao grupo controle negativo. Com a realização do modelo in vivo do TAE, observou-se ainda que o efeito antitumoral promovido por esses extratos é dose-dependente, uma vez que se apresentou-maior, quanto mais elevada a concentração de extrato administrada ao animal. A realização do ensaio do CAM, permitiu verificar que tanto o EB-ETOH, como f-CHCl3 de C. sylvetris, exercem um significativo efeito antiangiogênico, de maneira dose-dependente, onde a administração de 3,25; 7,5 e 15 µg/disco de EB-ETOH e f-CHCl3 foram capazes de diminuir o percentual de vasos, em torno de 54,5; 61,4 e 71% e 42,1; 59,1 e 70,9%, respectivamente. Por fim, cabe ressaltar que a realização da biometria dos embriões possibilitou observar que o tratamento dos mesmos com extrato bruto e fração clorofórmica não se apresentou tóxico, uma vez que não alterou o desenvolvimento embrionário nem a morfogênese externa, avaliados pelo comprimento total do embrião, razão comprimento cefálico/comprimento truncal e razão comprimento cefálico/comprimento total. Dessa forma, conclui-se que EB-ETOH e f-CHCl3 de C. sylvestris apresentaram efeito citotóxico, antiproliferativo, pró apoptótico e antitumoral in vitro e in vivo, sobre as células do carcinoma de Ehrlich. Tal efeito antitumoral mediado por esses extratos, não envolvem sua intercalação ao DNA, e sugere-se que se deva, pelo menos em parte, à inibição da formação de vasos e a conseqüente supressão nutricional das células tumorais
From user behaviours to collective semantics
No full textThe World Wide Web has developed into an important platform for social interactions with the rise of social networking applications of different kinds. Collaborative tagging systems, as prominent examples of these applications, allow users to share their resources and to interact with each other. By assigning tags to resources on the Web in a collaborative manner, users contribute to the emergence of complex networks now commonly known as folksonomies, in which users, documents and tags are interconnected with each other. To reveal the implicit semantics of entities involved in a folksonomy, one requires an understanding of the characteristics of the collective behaviours that create these interconnections. This thesis studies how user behaviours in collaborative tagging systems can be analysed to acquire a better understanding of the collective semantics of entities in folksonomies. We approach this problem from three different but closely related perspectives. Firstly, we study how tags are used by users and how their different intended meanings can be identified. Secondly, we develop a method for assessing the expertise of users and quality of documents in folksonomies by introducing the notion of implicit endorsement. Finally, we study the relations between documents induced from collaborative tagging and compare them with existing hyperlinks between Web documents. We show that, in each of these scenarios, it is crucial to consider the collective behaviours of the users and the social contexts in order to understand the characteristics of the entities. This project can be considered as a case study of the Social Web, the research outcomes of which can be easily generalised to many other social networking applications. It also fits into the larger framework for understanding the Web set out by the emerging interdisciplinary field of Web Science, as the work involves analyses of the interactions and behaviour of Web users in order to understand how we can improve existing systems and facilitate information sharing and retrieval on the Web
The effect of in vitro culture on the stability, expansion and neuronal differentiation of human pluripotent cell lines
No full textPluripotent cells are defined by their ability to both self-renew and to differentiation into any cell type within the human body. As such, pluripotent cell lines are of great interest as starting material for drug screening and cell therapies for regenerative treatment of diseased tissues. Pluripotent cell lines were originally derived from germ cell tumors (embryonal carcinoma cells; EC), but have since been isolated and expanded from the inner cell mass of an early embryo (human embryonic stem cells; hESCs).
This project set out to investigate the relative ability of the pluripotent NTERA2 (EC) cell line and hESC lines: Shef3, HUES7 and RH5, to differentiate into neurons, using mechanical and enzymatic culture methods. Focus was placed on monitoring differentiation efficiency and function between the different lines.
The tumour origin, in addition to the poor reproducibility, low yield and reduced functionality of NTERA2 derived neurons, compared to primary neurons, makes their incorporation into regenerative therapies unlikely. As such, an enhanced neuronal differentiation protocol was developed for use in hESCs. Cell populations were monitored for relative changes in gene and protein expression at selected time points throughout differentiation using standard RT-PCR, Q-PCR and immuno fluorescence analysis. End stage neurons were screened for functionality using patch clamping and calcium imaging techniques. Monitoring of cellular behavior through differentiation was aided by the concurrent development of a portable microscope incubator stage in collaboration with Linkam scientific Ltd.
These data demonstrate a variation in the ability to generate neurons from pluripotent cell lines, and suggests a predetermined, preferential cell fate within each line, even at the level of pluripotency. This study also characterises in detail neuronal differentiation from pluripotent cells, adding to the understanding which is essential for translation into therapies for neurodegenerative diseases such as Parkinson’s, Alzheimer’s, and Huntingdon’s disease
Positive communication for decreasing burnout in intensive-care-unit staff: a cluster-randomized trial.
No full textPurpose: Occupational burnout is common among intensive-care-unit (ICU) staff and adversely affects staff well-being and patient care. We hypothesized that a multicomponent intervention based on organizational support and workplace climate improvement would reduce burnout. Methods: The 1:1 cluster-randomized Hello trial involved 370 ICUs from sixty countries allocated to either the intervention or usual care. The four-week intervention designed to promote a positive workplace culture and within-team support used posters, email nudges, greetings during morning meetings, role modeling, and positive messages in boxes and on noticeboards. The primary endpoint was burnout prevalence, measured using the Maslach Burnout Inventory. Secondary outcomes included MBI subscale scores, well-being, job satisfaction, ethical climate, intention to leave, work safety, and professional conflicts. Results: Before the intervention, burnout prevalence was 59.4% (95% CI, 58.6-60.5), with no difference between arms. After the intervention, 4966 intervention-arm and 4602 control-arm healthcare professionals completed the MBI. Burnout prevalence was significantly lower in the intervention arm relative to controls (52.2% vs. 63.3%; adjusted odds ratio, 0.56; 95%CI 0.46-0.68; P < 0.001). Among MBI sub-scales scores, emotional exhaustion and depersonalization were lower, and personal accomplishment was higher in the intervention arm. Staff in the intervention arm reported better job satisfaction, workplace safety, ethical climate, and patient- and family-centered care; they were less often considering a job change. Conclusions: The Hello intervention reduced burnout and improved workplace culture among ICU staff. Given the pragmatic design, the intervention tested may have broad applicability. Trial registration: The trial was registered on ClinicalTrials.gov on June 18, 2024 (NCT06453616)
