2,799 research outputs found
Cadherin-9 is a novel cell surface marker for the heterogeneous pool of renal fibroblasts.
No full textBackgroundInterstitial fibroblasts are a minor, but nevertheless very important, component of the kidney. They secrete and remodel extracellular matrix and they produce active compounds such as erythropoietin. However, studying human renal fibroblasts has been hampered by the lack of appropriate surface markers.Methods and findingsThe expression of cadherin-9 in various human renal cell lines and tissues was studied on the mRNA level by RT-PCR and on the protein level with the help of newly generated cadherin-9 antibodies. The classical type II cadherin-9, so far only described in the neural system, was identified as a reliable surface marker for renal fibroblasts. Compared to FSP1, a widely-used cytosolic renal fibroblast marker, cadherin-9 showed a more restricted expression pattern in human kidney. Under pathological conditions, cadherin-9 was expressed in the stroma of renal cell carcinoma, but not in the tumor cells themselves, and in renal fibrosis the percentage of cadherin-9-positive cells was clearly elevated 3 to 5 times compared to healthy kidney tissue. Induction of epithelial mesenchymal transition in renal epithelial cells with cyclosporin-A, which causes renal fibrosis as a side effect, induced cadherin-9 expression. Functional studies following siRNA-mediated knockdown of cadherin-9 revealed that it acts in the kidney like a typical classical cadherin. It was found to be associated with catenins and to mediate homophilic but not heterophilic cell interactions.ConclusionsCadherin-9 represents a novel and reliable cell surface marker for fibroblasts in healthy and diseased kidneys. Together with the established marker molecules FSP1, CD45 and alpha smooth muscle actin, cadherin-9 can now be used to differentiate the heterogenic pool of renal fibroblasts into resident and activated fibroblasts, immigrated bone marrow derived fibroblast precursors and cells in different stages of epithelial mesenchymal transition
Urocortin and corticotropin-releasing factor receptor 2 in human renal cell carcinoma : disruption of an endogenous inhibitor of angiogenesis and proliferation
No full textUrocortin (Ucn) exerts its actions through activation of two corticotropin-releasing factor receptors (CRFRs), CRFR1 and CRFR2. Involvement of Ucn/CRFR2 system in pathophysiological conditions such as the regulation of angiogenesis and inhibition of proliferation has been already reported. Suppression of neovascularization through reduction of vascular endothelial growth factor and inhibition of tumor cell cycling is modulated mainly through activation of CRFR2. To find out a possible involvement of Ucn/CRFR2 in kidney tumor, we examined the expression of Ucn and CRFR2 in normal and tumoral kidney specimens
Differential expression of urocortin in human testicular germ cells in course of spermatogenesis: role for urocortin in male fertility ?
No full textTo provide the first insights into the potential role of urocortin in mammalian spermatogenesis, we studied the expression of urocortin and corticotropin-releasing factor receptors 1 and 2 in the human testis. Urocortin is a bioactive peptide with antiapoptotic and antiproliferative properties. The proper regulation of apoptosis and proliferation is of high physiologic relevance in the control of spermatogenesis in adulthood and of the noncycling stage of gonocytes in fetal life
Evidence based leadership
No full textAuthor Markus PodduikinMasterarbeit Johannes Kepler Universität Linz 2024Arbeit gesperr
Improving the search for monitoring tools using recommender technology
No full textAuthor Markus Eisl BSc.Masterarbeit Universität Linz 2023Arbeit gesperr
Improving the search for monitoring tools using recommender technology
No full textAuthor Markus Eisl BSc.Masterarbeit Universität Linz 2023Arbeit gesperr
metal-organic framework 4H, 4H-octafluoroazobenezene perfluoroazobenzene
No full textauthor: Markus RödlKumulative Masterarbeit aus einem ArtikelMasterarbeit University of Innsbruck 2022Arbeit gesperr
metal-organic framework 4H, 4H-octafluoroazobenezene perfluoroazobenzene
No full textauthor: Markus RödlKumulative Masterarbeit aus einem ArtikelMasterarbeit University of Innsbruck 2022Arbeit gesperr
Strategi Pelayanan Lintas Budaya Berdasarkan Markus 4:1-34
No full textHarming & Katarina, Cross-cultural Service Strategy based on Mark 4: 1-34. The author conducted a study of the text of Mark 4: 1-34 using qualitative research literature study using the biblical hermeneutic principle. Based on the analysis of the text of Mark 4: 1-34 the authors find there are four cross-cultural service strategies namely by understanding local culture, using media. Harming & Katarina, Strategi Pelayanan Lintas budaya Berdasarkan Markus 4:1-34. Penulis melakukan penelitian terhadap teks Markus 4:1-34 dengan menggunakan penelitian kualitatif studi pustaka dengan menggunakan prinsip hermeneutik Alkitab. Berdasarkan analisis terhadap teks Markus 4:1-34 penulis menemukan ada empat strategi pelayanan lintas budaya yaitu dengan memahami budaya lokal, menggunakan media yang ada dalam budaya, memberdayakan potensi yang ada, dan menciptakan terobosan
- …
